ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV6208 good E KOG1368 Amino acid transport and metabolism Threonine aldolase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV6208 2134462 2133284 -393
(393 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YEL046c [E] KOG1368 Threonine aldolase 312 5e-85
SPAC23H3.09c [E] KOG1368 Threonine aldolase 194 1e-49
CE23914 [E] KOG1368 Threonine aldolase 125 1e-28
CE23913 [E] KOG1368 Threonine aldolase 125 1e-28
At3g04520 [E] KOG1368 Threonine aldolase 122 1e-27
At1g08630 [E] KOG1368 Threonine aldolase 114 2e-25
7301016 [E] KOG1368 Threonine aldolase 106 7e-23
>YEL046c [E] KOG1368 Threonine aldolase
Length = 387
Score = 312 bits (800), Expect = 5e-85
Identities = 166/382 (43%), Positives = 238/382 (61%), Gaps = 32/382 (8%)
Query: 3 IPPLYTSSAGDFRSDTFTIPTKDFWSQVTDASLGDTINLEDTDTCSLEARLSDEILGGRV 62
+PP Y ++A D RSDTFT PT + +AS+GD + ED DT LE ++
Sbjct: 6 LPPKYITAANDLRSDTFTTPTAEMMEAALEASIGDAVYGEDVDTVRLEQTVA-------- 57
Query: 63 HSGEKSEGLFCMSTTLANQIAVRSHLNFAPPYSVLCDKRAHIFVDECAGIAVLSQALVMP 122
K GLFC+S TL+NQIA+R+HL PPYS+LCD RAH++ E AG+A+LSQA+V+P
Sbjct: 58 RMAGKEAGLFCVSGTLSNQIAIRTHL-MQPPYSILCDYRAHVYTHEAAGLAILSQAMVVP 116
Query: 123 TMASNGCYLTLEDIKENYVPDIGDIHLAPTRLICLENTLHGMLFPLTELQRISDWAHSQD 182
+ SNG YLTLEDIK +YVPD GDIH APTRLI LENTLHG+++PL EL RI W
Sbjct: 117 VVPSNGDYLTLEDIKSHYVPDDGDIHGAPTRLISLENTLHGIVYPLEELVRIKAWCMENG 176
Query: 183 IKIHLDGARLWXXXXXXXXXXXXXXEFETEIEYMQKVSKLVDSVSICLSKSIGAPIGSVL 242
+K+H DGAR+W +++ ++ DS+SICLSKS+GAPIGSVL
Sbjct: 177 LKLHCDGARIWNAAAQSGVP-------------LKQYGEIFDSISICLSKSMGAPIGSVL 223
Query: 243 VGSSQFIKKARHIQKQQGGGIRQAGFITKIANYCIDLNFPNRIVEVNKATSRFWTDLFHT 302
VG+ +F+KKA H +KQQGGGIRQ+G + ++A I+ ++ ++++ + L H
Sbjct: 224 VGNLKFVKKATHFRKQQGGGIRQSGMMARMALVNINNDWKSQLL--------YSHSLAHE 275
Query: 303 LKDQYQFE-LVLQHPVETNFIFVDXXXXXXXXXXXXEFAEKNNVRLWDGRLAFHYQNIDQ 361
L + + + + L+ P +TNF+F++ + K NV+L GR++FHYQ + +
Sbjct: 276 LAEYCEAKGIPLESPADTNFVFINLKAARMDPDVLVKKGLKYNVKLMGGRVSFHYQ-VTR 334
Query: 362 EGLDRLKQTFIDIAKYYKEHPY 383
+ L+++K + Y KEHP+
Sbjct: 335 DTLEKVKLAISEAFDYAKEHPF 356
>SPAC23H3.09c [E] KOG1368 Threonine aldolase
Length = 376
Score = 194 bits (494), Expect = 1e-49
Identities = 124/356 (34%), Positives = 183/356 (50%), Gaps = 36/356 (10%)
Query: 13 DFRSDTFTIPTKDFWSQVTDASLGDTINLEDTDTCSLEARLSDEILGGRVHSGEKSEGLF 72
DFRSDT T+PT + + +AS GD + ED DT LE ++ ++ G K LF
Sbjct: 30 DFRSDTLTVPTDEMRRIMYEASDGDCVYEEDEDTRKLEVYVA-KLTG-------KEAALF 81
Query: 73 CMSTTLANQIAVRSHLNFAPPYSVLCDKRAHIFVDECAGIAVLSQALVMPTMASNGCYLT 132
S T NQ+ +RSHL+ PP+S++CD RAHI+ E I + +QA+V P N Y+T
Sbjct: 82 VTSGTQGNQLCIRSHLH-QPPHSIICDDRAHIYNWEAGAIGLFTQAIVRPISPKNNVYIT 140
Query: 133 LEDIKENYVPDIGDIHLAPTRLICLENTLHGMLFPLTELQRISDWAHSQDIKIHLDGARL 192
E+I+ + DIH +PT LICLENT+ G + PL E+ RIS A + I +H DGARL
Sbjct: 141 AEEIENKLILG-NDIHFSPTGLICLENTIKGAVVPLDEVARISGLAKAHKIPLHCDGARL 199
Query: 193 WXXXXXXXXXXXXXXEFETEIEYMQKVSKLVDSVSICLSKSIGAPIGSVLVGSSQFIKKA 252
W +++ DSVS+CLSK + AP+GS++VG FI KA
Sbjct: 200 WDAAVASNVS-------------IKEYCSYFDSVSLCLSKGLAAPVGSIIVGPRDFIAKA 246
Query: 253 RHIQKQQGGGIRQAGFITKIANYCIDLNFPNRIVEVNKATSRFWTDLFHTLKDQYQFELV 312
+ +K GGG+RQ+G + Y I NFP + +V+K + +
Sbjct: 247 KWFRKAYGGGLRQSGMLAAAGLYSIQHNFP-LLKQVHKYA-------IEVAEYAESLGIE 298
Query: 313 LQHPVETNFIFVDXXXXXXXXXXXXEFAEKNNVRLWDGRLAFHYQNIDQEGLDRLK 368
L+ P ++N + + A+K+ + L R+ FH Q I + ++ LK
Sbjct: 299 LEVPTQSNMV----TLANINVAILCDEAKKSGIILMGPRIVFHIQ-ITPDAVEILK 349
>CE23914 [E] KOG1368 Threonine aldolase
Length = 433
Score = 125 bits (313), Expect = 1e-28
Identities = 84/292 (28%), Positives = 142/292 (47%), Gaps = 44/292 (15%)
Query: 8 TSSAGDFRSDTFTIPTKDFWSQVTDASLGDTINLEDTDTCSLEARLSDEILGGRVHSGEK 67
T ++ D RSDT T+P+ + + +A +GD + EDT T LE R + E+ G K
Sbjct: 63 THTSIDLRSDTVTVPSVEMRRAMAEAIVGDDVYGEDTTTNRLEQRCA-ELFG-------K 114
Query: 68 SEGLFCMSTTLANQIAVRSHLNFAPPYSVLCDKRAHIF------VDECAGIAVLSQAL-- 119
GLF S T+ N +A+ +H ++ + HI + AGI+ + +
Sbjct: 115 EAGLFVTSGTMGNLLAIMAHCQRGE--EIIVGRYNHIHRWEQGNYAQFAGISATTLEVKP 172
Query: 120 --------VMPTMASNGCYLTLED--IKENYVPDIGDIHLAPTRLICLENTLH---GMLF 166
+ + G Y+ ++D + V I D H+ ++LIC+ENT + G
Sbjct: 173 DGTMDLNDIEQAIRVKGGYVQIKDESCDDLEVTSIPDCHMPASKLICIENTHNYTGGKAL 232
Query: 167 PLTELQRISDWAHSQDIKIHLDGARLWXXXXXXXXXXXXXXEFETEIEYMQKVSKLVDSV 226
P+ ++ + A +D+K+H+DGAR++ + K++ D+V
Sbjct: 233 PIEWMRSVKQLAERRDLKVHMDGARIYNAAVASNCS-------------VSKIASFADTV 279
Query: 227 SICLSKSIGAPIGSVLVGSSQFIKKARHIQKQQGGGIRQAGFITKIANYCID 278
+C SK +GAP+GS++VG FI +ARH +K GGG RQ+G + A+ +D
Sbjct: 280 QMCFSKGLGAPVGSIVVGPKDFIDRARHSRKALGGGWRQSGILAAAAHIALD 331
>CE23913 [E] KOG1368 Threonine aldolase
Length = 428
Score = 125 bits (313), Expect = 1e-28
Identities = 84/292 (28%), Positives = 142/292 (47%), Gaps = 44/292 (15%)
Query: 8 TSSAGDFRSDTFTIPTKDFWSQVTDASLGDTINLEDTDTCSLEARLSDEILGGRVHSGEK 67
T ++ D RSDT T+P+ + + +A +GD + EDT T LE R + E+ G K
Sbjct: 58 THTSIDLRSDTVTVPSVEMRRAMAEAIVGDDVYGEDTTTNRLEQRCA-ELFG-------K 109
Query: 68 SEGLFCMSTTLANQIAVRSHLNFAPPYSVLCDKRAHIF------VDECAGIAVLSQAL-- 119
GLF S T+ N +A+ +H ++ + HI + AGI+ + +
Sbjct: 110 EAGLFVTSGTMGNLLAIMAHCQRGE--EIIVGRYNHIHRWEQGNYAQFAGISATTLEVKP 167
Query: 120 --------VMPTMASNGCYLTLED--IKENYVPDIGDIHLAPTRLICLENTLH---GMLF 166
+ + G Y+ ++D + V I D H+ ++LIC+ENT + G
Sbjct: 168 DGTMDLNDIEQAIRVKGGYVQIKDESCDDLEVTSIPDCHMPASKLICIENTHNYTGGKAL 227
Query: 167 PLTELQRISDWAHSQDIKIHLDGARLWXXXXXXXXXXXXXXEFETEIEYMQKVSKLVDSV 226
P+ ++ + A +D+K+H+DGAR++ + K++ D+V
Sbjct: 228 PIEWMRSVKQLAERRDLKVHMDGARIYNAAVASNCS-------------VSKIASFADTV 274
Query: 227 SICLSKSIGAPIGSVLVGSSQFIKKARHIQKQQGGGIRQAGFITKIANYCID 278
+C SK +GAP+GS++VG FI +ARH +K GGG RQ+G + A+ +D
Sbjct: 275 QMCFSKGLGAPVGSIVVGPKDFIDRARHSRKALGGGWRQSGILAAAAHIALD 326
>At3g04520 [E] KOG1368 Threonine aldolase
Length = 370
Score = 122 bits (305), Expect = 1e-27
Identities = 90/266 (33%), Positives = 132/266 (48%), Gaps = 29/266 (10%)
Query: 13 DFRSDTFTIPTKDFWSQVTDASLGDTINLEDTDTCSLEARLSDEILGGRVHSGEKSEGLF 72
D RSDT T PT+ S + +A + D + D LE ++ EI G K +F
Sbjct: 11 DLRSDTVTKPTESMRSAMANAEVDDDVLGNDPTALRLEKEVA-EIAG-------KEAAMF 62
Query: 73 CMSTTLANQIAVRSHLNFAPPYSVLCDKRAHIFVDECAGIAVLSQALVMPTMASNGCYLT 132
S T+ N I+V H + +L D +HI + E G++ L V P N T
Sbjct: 63 VPSGTMGNLISVLVHCDERGSEVILGDD-SHIHIYENGGVSSLGG--VHPRTVKNEEDGT 119
Query: 133 LE--DIKENYVPDIGDIHLAPTRLICLENT---LHGMLFPLTELQRISDWAHSQDIKIHL 187
+E I+ GD+H T+LICLENT G P+ + ++ + A +K+H+
Sbjct: 120 MEIGAIEAAVRSPKGDLHHPVTKLICLENTQANCGGRCLPIEYIDKVGELAKKHGLKLHI 179
Query: 188 DGARLWXXXXXXXXXXXXXXEFETEIEYMQKVSKLVDSVSICLSKSIGAPIGSVLVGSSQ 247
DGAR++ ++++ + DSVSICLSK IGAP+GSV+VGS +
Sbjct: 180 DGARIFNASVALGVP-------------VKRIVQAADSVSICLSKGIGAPVGSVIVGSKK 226
Query: 248 FIKKARHIQKQQGGGIRQAGFITKIA 273
FI KAR ++K GGG+RQ G + A
Sbjct: 227 FITKARWLRKTLGGGMRQIGVLCAAA 252
>At1g08630 [E] KOG1368 Threonine aldolase
Length = 484
Score = 114 bits (286), Expect = 2e-25
Identities = 85/273 (31%), Positives = 124/273 (45%), Gaps = 25/273 (9%)
Query: 13 DFRSDTFTIPTKDFWSQVTDASLGDTINLEDTDTCSLEARLSDEILGGRVHSGEKSEGLF 72
D RSDT T PT + +A + D + D LE ++ +++G K LF
Sbjct: 91 DLRSDTVTRPTDAMREAMCNAEVDDDVLGYDPTARRLEEEMA-KMMG-------KEAALF 142
Query: 73 CMSTTLANQIAVRSHLNFAPPYSVLCDKRAHIFVDECAGIAVLSQALVMPTMASNGCYLT 132
S T+ N I+V H + +L D HI V E GI+ + +
Sbjct: 143 VPSGTMGNLISVMVHCDVRGSEVILGDN-CHIHVYENGGISTIGGVHPKTVKNEEDGTMD 201
Query: 133 LEDIKENYVPDIGDIHLAPTRLICLENT---LHGMLFPLTELQRISDWAHSQDIKIHLDG 189
LE I+ G TRLICLENT G + +++ + A +K+H+DG
Sbjct: 202 LEAIEAAIRDPKGSTFYPSTRLICLENTHANSGGRCLSVEYTEKVGEIAKRHGVKLHIDG 261
Query: 190 ARLWXXXXXXXXXXXXXXEFETEIEYMQKVSKLVDSVSICLSKSIGAPIGSVLVGSSQFI 249
ARL+ + K+ K DSV +CLSK +GAP+GSV+VGS FI
Sbjct: 262 ARLFNASIALGVP-------------VHKLVKAADSVQVCLSKGLGAPVGSVIVGSQSFI 308
Query: 250 KKARHIQKQQGGGIRQAGFITKIANYCIDLNFP 282
+KA+ ++K GGG+RQ G + A + N P
Sbjct: 309 EKAKTVRKTLGGGMRQIGVLCAAALVALQENLP 341
>7301016 [E] KOG1368 Threonine aldolase
Length = 383
Score = 106 bits (264), Expect = 7e-23
Identities = 92/318 (28%), Positives = 140/318 (43%), Gaps = 56/318 (17%)
Query: 8 TSSAGDFRSDTFTIPTKDFWSQVTDASLGDTINLEDTDTCSLEARLSDEILGGRVHSGEK 67
T S D RSDT + PT +++ A +GD + ED LEAR + I G K
Sbjct: 3 THSVVDLRSDTVSQPTDKMRARMATAVVGDDVYGEDPTVNELEARTA-AIFG-------K 54
Query: 68 SEGLFCMSTTLANQIAVRSHLNFAPPYSVLCDKRAHIFVDECAGIAVLSQALVMPTMASN 127
GLF S T+ N +A+ H + +++ D +HIF+ E G + L+ +
Sbjct: 55 EAGLFVPSGTMGNLLAIMVHCHRRGTEALVGDL-SHIFLYEQGGASHLAGVQLATLKNEQ 113
Query: 128 GCYLTLEDIKENYVPDIGDIHLAPTRLICLENTLH---GMLFPLTELQRISDWAHSQD-- 182
+L+D++ + D H T L+ +E T + G + PL L ++
Sbjct: 114 DGTFSLQDLRRRIRHE--DCHEPITSLVVVEQTHNICGGKVVPLAFLDELAALVRQPGVG 171
Query: 183 -----IKIHLDGARLWXXXXXXXXXXXXXXEFETEIEYMQKVSKLVDSVSICLSKSIGAP 237
I +H+DGAR++ ++++ + DSVSICLSK + AP
Sbjct: 172 TGNARIALHMDGARVFNAATALGVG-------------VERICRDFDSVSICLSKGLSAP 218
Query: 238 IGSVLVGSSQFIKKARHIQKQQGGGIRQAGFI----------------------TKIANY 275
+GSVLVGS FI +A ++K GGG+RQ G + KIAN
Sbjct: 219 VGSVLVGSKAFISEAHRLRKALGGGMRQVGVLAAAGLVALDEVVPLLSKDHERTNKIANA 278
Query: 276 CIDLNFPNRIVEVNKATS 293
+L PN V V+ S
Sbjct: 279 IYELKSPNITVHVSSVQS 296
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.321 0.137 0.413
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,392,161
Number of Sequences: 60738
Number of extensions: 827491
Number of successful extensions: 1795
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1766
Number of HSP's gapped (non-prelim): 7
length of query: 393
length of database: 30,389,216
effective HSP length: 108
effective length of query: 285
effective length of database: 23,829,512
effective search space: 6791410920
effective search space used: 6791410920
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)