ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV6212 good I KOG2329 Lipid transport and metabolism Alkaline ceramidase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV6212 2135856 2134894 -321
(321 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPL087w [I] KOG2329 Alkaline ceramidase 299 4e-81
YBR183w [I] KOG2329 Alkaline ceramidase 297 1e-80
Hs19923527 [I] KOG2329 Alkaline ceramidase 125 8e-29
Hs19424128 [I] KOG2329 Alkaline ceramidase 64 3e-10
At4g22330_2 [I] KOG2329 Alkaline ceramidase 62 9e-10
7298654 [I] KOG2329 Alkaline ceramidase 61 2e-09
>YPL087w [I] KOG2329 Alkaline ceramidase
Length = 317
Score = 299 bits (765), Expect = 4e-81
Identities = 147/299 (49%), Positives = 191/299 (63%), Gaps = 3/299 (1%)
Query: 12 YPTPPESGFWGTPTSTIDWCEENYVISPYIAEWSNTLTNSGFILLALYLLYSSWKNKLET 71
YP P G+WG PTS IDWCEENYV+SPYIAEWSNT+TNS F++ A Y YS+W+NKLET
Sbjct: 7 YPEAPIEGYWGKPTSLIDWCEENYVVSPYIAEWSNTITNSIFLMTAFYSTYSAWRNKLET 66
Query: 72 RFKLVCAGFGLVGIGSWLFHMTLQYKYQLLDELPMVYATCIPAWSIFCE--EIDVATSRI 129
R+ L+ GF LVGIGSWLFHMTLQY+YQLLDELPM+YAT IP+WSIF E EI + +
Sbjct: 67 RYILIGMGFSLVGIGSWLFHMTLQYRYQLLDELPMLYATIIPSWSIFAETQEILIKDEKK 126
Query: 130 RSPTR-RKQWTVGLTIFMGANLLTAIYLIFKNPTIHQAGYALINAIVIWFAFKLTTQFVN 188
R + R Q + + +LT IY++ + P I Q Y ++ +V+ + LT V+
Sbjct: 127 RKESSFRIQMVISFIMCGIVTILTWIYVVVQKPAIFQVLYGILTLLVVVLSGWLTYYHVH 186
Query: 189 DPVAKRNLQNAMLLGITIFLAGYFVWQLDVHFCQFWITIRRSYLRLPXXXXXXXXXXXXX 248
D AK+NL M++G+ F+ G+ WQLD+H C FWI IRR+YL LP
Sbjct: 187 DSFAKKNLFITMVMGMIPFVIGFICWQLDIHLCSFWIYIRRTYLALPLGVLLELHAWWHL 246
Query: 249 XXXXXXYFYIVYLEYLRIYIHGKRDEYEMIWRWKVLPEVVIKTSHISTPFSHEFLGSCV 307
Y ++VYL+YLRI HG +++ IWRW PE+V K I T +S E+LG V
Sbjct: 247 LTGTGVYIFVVYLQYLRILTHGNPNDFLFIWRWGFFPELVRKGLPIGTSYSLEYLGPIV 305
>YBR183w [I] KOG2329 Alkaline ceramidase
Length = 316
Score = 297 bits (761), Expect = 1e-80
Identities = 150/304 (49%), Positives = 194/304 (63%), Gaps = 3/304 (0%)
Query: 7 GIFR-DYPTPPESGFWGTPTSTIDWCEENYVISPYIAEWSNTLTNSGFILLALYLLYSSW 65
GIFR +YP G WG TSTIDWCEENYV+SPYIAEWSNTLTNS FIL A+Y YS++
Sbjct: 2 GIFRWNYPESSVPGVWGETTSTIDWCEENYVVSPYIAEWSNTLTNSVFILSAIYTTYSAY 61
Query: 66 KNKLETRFKLVCAGFGLVGIGSWLFHMTLQYKYQLLDELPMVYATCIPAWSIFCE--EID 123
KNKLE RF L+ G+GLVG+GSWLFHMTL+Y++QLLDELPM+YA CIP WS+ CE E
Sbjct: 62 KNKLEKRFLLIGFGYGLVGVGSWLFHMTLKYRFQLLDELPMIYAMCIPTWSLVCEAKEAL 121
Query: 124 VATSRIRSPTRRKQWTVGLTIFMGANLLTAIYLIFKNPTIHQAGYALINAIVIWFAFKLT 183
+ + +Q +G+ I + + +Y+I+KN IHQ + + +V A LT
Sbjct: 122 LNGDNHKKVPLFEQIFIGVIIGLAVTTASILYVIYKNVDIHQILFGVQIVVVAATAGSLT 181
Query: 184 TQFVNDPVAKRNLQNAMLLGITIFLAGYFVWQLDVHFCQFWITIRRSYLRLPXXXXXXXX 243
++V+DP+AKRNL+ +M LG +FL+GY W LD+H+C FW+ +RRS L LP
Sbjct: 182 YRYVHDPLAKRNLKASMALGAILFLSGYISWLLDIHYCSFWVHVRRSILALPLGVLLEPH 241
Query: 244 XXXXXXXXXXXYFYIVYLEYLRIYIHGKRDEYEMIWRWKVLPEVVIKTSHISTPFSHEFL 303
YFYIV LE+LR+ Y+ IWRWKV PE++ K ST +S E
Sbjct: 242 GWWHILTGMGIYFYIVSLEHLRVITLNVSCNYQFIWRWKVFPELIWKGRKPSTRYSLELF 301
Query: 304 GSCV 307
G V
Sbjct: 302 GPYV 305
>Hs19923527 [I] KOG2329 Alkaline ceramidase
Length = 267
Score = 125 bits (314), Expect = 8e-29
Identities = 70/211 (33%), Positives = 113/211 (53%), Gaps = 17/211 (8%)
Query: 13 PTPPESGFWGTPTSTIDWCEENYVISPYIAEWSNTLTNSGFILLALYLLYSSWKNKLETR 72
P G+WG TST+DWCEENY ++ YIAE+ NT++N I+ ++ S ++ LE R
Sbjct: 3 PAADREGYWGPTTSTLDWCEENYSVTWYIAEFWNTVSNLIMIIPPMFGAIQSVRDGLEKR 62
Query: 73 FKLVCAGFGLVGIGSWLFHMTLQYKYQLLDELPMVYATCIPAWSIF-CEEIDVATSRIRS 131
+ +VG+GSW FHMTL+Y+ QLLDELPM+Y+ CI + +F C +I + +
Sbjct: 63 YIASYLALTVVGMGSWCFHMTLKYEMQLLDELPMIYSCCIFVYCMFECFKIKNSVN---- 118
Query: 132 PTRRKQWTVGLTIFMGANLLTAIYLIFKNPTIHQAGYALINAIVIWFAFKLTTQFVNDPV 191
+ + T+ + + ++T +YL K P HQ Y ++ ++ + + T
Sbjct: 119 ------YHLLFTLVLFSLIVTTVYLKVKEPIFHQVMYGMLVFTLVLRSIYIVTW------ 166
Query: 192 AKRNLQNAMLLGITIFLAGYFVWQLDVHFCQ 222
L+ + IFL G+ W +D FC+
Sbjct: 167 VYPWLRGLGYTSLGIFLLGFLFWNIDNIFCE 197
>Hs19424128 [I] KOG2329 Alkaline ceramidase
Length = 264
Score = 63.9 bits (154), Expect = 3e-10
Identities = 54/221 (24%), Positives = 94/221 (42%), Gaps = 37/221 (16%)
Query: 25 TSTIDWCEENYVISPYIAEWSNTLTNSGFILLA--LYLLYSSWKNKLETRFKLVCAGFGL 82
+S +DWCE N+ S +AE+ NT +N F + + LL + K +V F +
Sbjct: 9 SSEVDWCESNFQYSELVAEFYNTFSNIPFFIFGPLMMLLMHPYAQKRSRYIYVVWVLFMI 68
Query: 83 VGIGSWLFHMTLQYKYQLLDELPMVYATCIPAWSIFCEEIDVATSRIRSPTRRKQWTVGL 142
+G+ S FHMTL + QLLDE+ +++ + + R P+
Sbjct: 69 IGLFSMYFHMTLSFLGQLLDEIAILW--------LLGSGYSIWMPRCYFPS--------- 111
Query: 143 TIFMGANLLTAIYLI-----------FKNPTIHQAGYALINAIVIWFAFKLTTQFVNDPV 191
F+G N I L+ F PT++ YAL N+I + + + ++
Sbjct: 112 --FLGGNRSQFIRLVFITTVVSTLLSFLRPTVN--AYAL-NSIALHILYIVCQEY--RKT 164
Query: 192 AKRNLQNAMLLGITIFLAGYFVWQLDVHFCQFWITIRRSYL 232
+ + L++ + + + ++ W D C FW I YL
Sbjct: 165 SNKELRHLIEVSVVLWAVALTSWISDRLLCSFWQRIHFFYL 205
>At4g22330_2 [I] KOG2329 Alkaline ceramidase
Length = 234
Score = 62.4 bits (150), Expect = 9e-10
Identities = 50/189 (26%), Positives = 83/189 (43%), Gaps = 17/189 (8%)
Query: 34 NYVISPYIAEWSNTLTNSGFILLALYLLYSSWKNKLETRFKLVCAGFGLVGIGSWLFHMT 93
NY S YIAE+ NT++N ILLAL L ++ + + E RF ++ ++ IGS L+H T
Sbjct: 1 NYAYSSYIAEFYNTISNVPGILLALIGLVNALRQRFEKRFSILHISNMILAIGSMLYHAT 60
Query: 94 LQYKYQLLDELPMVYATCIPAWSIFCEEIDVATSRIRSPTRRKQWTVGLTIFMGANLLTA 153
LQ+ Q DE PMV+ + + ++ SP + T+ +F+
Sbjct: 61 LQHVQQQSDETPMVWEILLYMYILY------------SPDWHYRSTMPTFLFLYGAAFAI 108
Query: 154 IYLIFKNPTIHQAGYALINAIVIWFAFKLTTQFVNDPVAKRNLQNAMLLGITIFLAGYFV 213
++ + + Y ++ + I +K D AKR + + L G
Sbjct: 109 VHAYLRFGIGFKVHYVILCLLCIPRMYKYYIH-TEDTAAKRIAK----WYVATILVGSIC 163
Query: 214 WQLDVHFCQ 222
W D FC+
Sbjct: 164 WFCDRVFCK 172
>7298654 [I] KOG2329 Alkaline ceramidase
Length = 178
Score = 61.2 bits (147), Expect = 2e-09
Identities = 37/96 (38%), Positives = 56/96 (57%), Gaps = 3/96 (3%)
Query: 25 TSTIDWCEENYVISPYIAEWSNTLTNSGFILL--ALYLLYSSWKNKLETRFKLVCAGFGL 82
+S +DWCE NY+IS IAE+ NT +N FILL L +L+ + + ++ +
Sbjct: 24 SSPVDWCEGNYLISSNIAEFVNTFSNFLFILLPPVLIMLFKEYGRFVTPGIHVIWVLLIV 83
Query: 83 VGIGSWLFHMTLQYKYQLLDELPMVYATCIPAWSIF 118
VG+ S FH TL QLLDEL +++ + A+S+F
Sbjct: 84 VGLSSMYFHATLSLIGQLLDELAILW-VFMAAFSLF 118
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.327 0.141 0.469
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,616,155
Number of Sequences: 60738
Number of extensions: 781442
Number of successful extensions: 2014
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 2001
Number of HSP's gapped (non-prelim): 7
length of query: 321
length of database: 30,389,216
effective HSP length: 106
effective length of query: 215
effective length of database: 23,950,988
effective search space: 5149462420
effective search space used: 5149462420
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)