ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV6282 good O KOG0448 Posttranslational modification, protein turnover, chaperones Mitofusin 1 GTPase, involved in mitochondrila biogenesis
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV6282 2154452 2156926 825
(825 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YBR179c [O] KOG0448 Mitofusin 1 GTPase involved in mitochondrila... 717 0.0
SPBC1706.03 [O] KOG0448 Mitofusin 1 GTPase involved in mitochond... 351 2e-96
CE02898 [O] KOG0448 Mitofusin 1 GTPase involved in mitochondrila... 52 3e-06
>YBR179c [O] KOG0448 Mitofusin 1 GTPase involved in mitochondrila biogenesis
Length = 855
Score = 717 bits (1850), Expect = 0.0
Identities = 383/771 (49%), Positives = 511/771 (65%), Gaps = 12/771 (1%)
Query: 67 SHLTQQSYSLNRNSLLDSITVVRPLINDLITENNQRAIYIP--EHSESLDVLELKVKLDG 124
S L+Q +Y+ NR L SI + ++ L ENN R I+I + E L VL+L +KLDG
Sbjct: 85 SQLSQWNYNNNRVLLKRSILKTQAFMDQLQEENNIRPIFIAANDEREKLHVLQLNIKLDG 144
Query: 125 RENMQ------LDKSALAQLFKTQALSAIDHLINLQTRVQDTSSKVFITGDLNSGKSTLC 178
+ N + ++K AL++LF +Q +S +HL L+ RV D SSKVFITGD+N+GKS LC
Sbjct: 145 QYNTKEKNGFNIEKKALSKLFHSQIVSVTNHLNALKKRVDDVSSKVFITGDVNTGKSALC 204
Query: 179 NAFLRKKVLPEDQLPCTNVFCEILEARENGNMERVHAIPKTVATNVKDASVLYDMRDRST 238
N+ L++++LPEDQLPCTNVF EILEAREN +E VHAIP +A +K+A +Y +++ T
Sbjct: 205 NSLLKQRLLPEDQLPCTNVFSEILEARENDGIEEVHAIPLNIAPTLKEAIDMYSIQNPKT 264
Query: 239 YEDYTLDKLDQLVYDNDHYILLKIYIKDDKRPVDSSLLRNGTADISLIDSPGLNMDSVKT 298
YE +TL +L LV N Y LLKIYIKDDKRP +SLLRNGT DISLIDSPGLNMDS++T
Sbjct: 265 YEIHTLKELPDLVPQNGKYALLKIYIKDDKRPASTSLLRNGTVDISLIDSPGLNMDSLQT 324
Query: 299 TEVMSRQEEIDLVVFVVNAENQLTLSAREFITMASREKKLMFFVINKFDHIKDKDRCKKL 358
EVMSRQEEIDLV+FVVNAENQLTLSA+EFI++ASREKKLMFFV+ KFD I+DK RCK+L
Sbjct: 325 AEVMSRQEEIDLVIFVVNAENQLTLSAKEFISLASREKKLMFFVVKKFDKIRDKQRCKEL 384
Query: 359 ILDQIKEISPETYKQNSEFVHFVTSHG-KIPH---EXXXXXXXXXXXXXXXXXXXKEDPD 414
IL QI+++SPETYK+ ++FVHFV+ +G ++PH E DPD
Sbjct: 385 ILKQIRDLSPETYKRAADFVHFVSKNGDELPHYHNENDNEDHGDRKPDDDPYSSSDPDPD 444
Query: 415 FDKLEDNLRNFVFKKRSLSKLLPAKTYLIKLLKDLERISQCNLEKYKEEDYHLNALLIDL 474
FD LED+LRNFV KKRSLSKLLPAKTYL KLL D+ IS+ N++ Y EE+ +N L L
Sbjct: 445 FDSLEDSLRNFVLKKRSLSKLLPAKTYLSKLLSDIIMISKSNMKMYSEEEIKINEQLETL 504
Query: 475 QPELHDVTNRCSELIESIDKICEFTTKEVYGYTKMQINKALILKPAEFPPYSGLSGIYDY 534
+PE+ +C++L S+D++ E T Y TK + AL + E+P Y GL IYD+
Sbjct: 505 RPEILSARAKCNDLTTSVDQMAEQTITMTYNNTKEALLNALDVPLHEYPKYQGLGQIYDF 564
Query: 535 VQRVRTFIVDQILASVQVSEAYAKNSAEQSVSQINALAKEQLGDEFMAGKVFKSGLMFTK 594
+ FI +QI S+ SE +AK + V +I + K +LGD+FM +VF+S LMF K
Sbjct: 565 IFSTEAFIANQIDESIGSSELFAKQKTDLLVKKIYEIGKNELGDDFMCERVFRSELMFRK 624
Query: 595 KKHNLSKKLNIPLELHNFLDPSVEGFISYLSWGLVKPTPKITNEENTLTTKSSWSQSLAL 654
+KH + K+L + L + + P+ +GF+SYLSW P E T + + L L
Sbjct: 625 RKHLIGKRLKVSLSITDLFAPTWKGFLSYLSWQKPVTAPLPDIEGQTNEGQIGLMKYLGL 684
Query: 655 TSYSVSQYWTNPSLIFTSKIPTLLVYSFGGSKMITNIVLHGSRFFXXXXXXXXXXXXXXX 714
+Y ++QYW+ PSL+FTSKIPTL +Y G +K++ NI+L+G +
Sbjct: 685 KNYPLTQYWSRPSLLFTSKIPTLTLYFLGSTKVVGNIILNGIKLSSWSSLKKLSVPVIVV 744
Query: 715 XXXXXXAYLIHDLPRALPMKLSLKYKLRLQEIDYAHNNADRISKEVSQVFKYPTREVVKA 774
YLIHDLPRALPM LS+KYK +LQE+DY H NA R S EV V + PTRE++++
Sbjct: 745 GSLLGLTYLIHDLPRALPMNLSIKYKRKLQELDYIHLNAQRTSNEVRDVLRVPTREILRS 804
Query: 775 CELIVDKKTSQKKEVECKVRDNAMSIEFFQALLSKSQKQRGTVESINLEVD 825
CE+I+DKK KKE+E K N +SI+FFQ+L + Q+ VE INL++D
Sbjct: 805 CEIIMDKKQITKKELENKKESNLLSIKFFQSLYEGTVAQKLMVEEINLDID 855
>SPBC1706.03 [O] KOG0448 Mitofusin 1 GTPase involved in mitochondrila biogenesis
Length = 758
Score = 351 bits (901), Expect = 2e-96
Identities = 251/783 (32%), Positives = 387/783 (49%), Gaps = 100/783 (12%)
Query: 71 QQSYSLNRNSLLDSITVVRPLINDLIT------------------ENNQRAIYIPEHSES 112
Q Y+ NRN LL SI +++ L+N+L ++ Y P
Sbjct: 40 QFEYNQNRNQLLRSIHIIQNLLNELDNYVDRSDCLFHSVWRTDKEKSKFSGNYYPFSPSK 99
Query: 113 LDVLELKVKL------DGRENMQLDKSALAQLFKTQALSAIDHLINLQTRVQDTSSKVFI 166
++V+ + + L D + QL + A L K A HL +L +RV+DTSSK+ I
Sbjct: 100 MNVITIDLSLRSSSTADEKLISQLGEEAHESLLKVHIEKANKHLFSLFSRVEDTSSKILI 159
Query: 167 TGDLNSGKSTLCNAFLRKKVLPEDQLPCTNVFCEILEARENGNMERVHAIPKTVATNVKD 226
TGDLN+GKSTLCNA + K +LPEDQ PCT VFCE+ +A N + VHAIP
Sbjct: 160 TGDLNAGKSTLCNALVHKDILPEDQQPCTEVFCEVHDAELNDGKDCVHAIPH-------- 211
Query: 227 ASVLYDMRDRSTYEDYTLDKLDQLVYDNDHYILLKIYIKDDKRPVDSSLLRNGTADISLI 286
+ Y D STY+ + ++ L +LVY+ +++ +L +Y+ D RP SLL NG DI+LI
Sbjct: 212 -GLTYSHTDSSTYKVFPIEDLKRLVYETENWSMLIVYVNDG-RPAHESLLHNGITDIALI 269
Query: 287 DSPGLNMDSVKTTEVMSRQEEIDLVVFVVNAENQLTLSAREFITMASREKKLMFFVINKF 346
D+PGLN DS+KTT V + QEEID+VVFVVNAEN TLSA +F+ AS EK +F ++NKF
Sbjct: 270 DAPGLNTDSMKTTSVFACQEEIDVVVFVVNAENHFTLSATDFLRNASTEKSHIFIIVNKF 329
Query: 347 DHIKDKDRCKKLILDQIKEISPETYKQNSEFVHFVTSH-GKIPHEXXXXXXXXXXXXXXX 405
D+I+DK+RCK+LIL+QI +SP T+ + VHFV+ + P+
Sbjct: 330 DNIRDKERCKRLILEQIHTLSPGTFADAKDLVHFVSCRVARDPNN--------------- 374
Query: 406 XXXXKED---PDFDKLEDNLRNFVFKKRSLSKLLPAKTYLIKLLKDLERISQCNLEKYKE 462
+ED F ++E++LR+F+ + RS SKL P + YL L+ D+ I + N++
Sbjct: 375 ----REDALYSSFFQMENSLRSFILENRSKSKLAPVRRYLSGLVGDILNICEYNIKLIDF 430
Query: 463 EDYHLNALLIDLQPELHDVTNRCSELIESIDKICEFTTKEVYGYTKMQINKAL-ILKPAE 521
+ HL L DL P+ V + + + + E T + + +T ++ A+ L
Sbjct: 431 DINHLQQRLTDLSPKFRKVKHEQQFTYQKNESLVEATVQSISQHTHSELEDAIDSLGSFA 490
Query: 522 FPPYSGLSGIYDYVQRVRTFIVDQILASVQVSEAYAKNSAEQSVSQINALAKEQLGDEFM 581
YSG Y Y VR + + + SE YA+ E++V I K+ +
Sbjct: 491 SVKYSGFFFAYQYAISVRDAMQQYLEEKLLESEDYARKRTEEAVLCIQKDVKDNFDSAVL 550
Query: 582 AGKVFKSGLMFTKK-KHNLSKKLNIPLELHNFLDPSVEGFISYLSWGLVKPTPKITNEEN 640
VF + MF KK + L K L L +F+D + + S L +
Sbjct: 551 --PVFHANQMFIKKHRLQLQKHFRFELGLLDFIDLDLTERLGTWSASLSTILLVLGKTTP 608
Query: 641 TLTTKSSWSQSLALTSYSVSQYWTNPSLIFTSKIPTLLVYSFGGSKMITNIVLHGSRFFX 700
+ TT +++ +L Y + +Y+ N SL LLV G + +I + G
Sbjct: 609 SFTTLGAFTGNL---GYPIFKYFQNNSL------QHLLVPVLG----LASICVFG----- 650
Query: 701 XXXXXXXXXXXXXXXXXXXXAYLIHDLPRALPMKLSLKYKLRLQEIDYAHNNADRISKEV 760
Y+I+D+PRALP+K++ K K L+E D+ HN + I E
Sbjct: 651 ---------------------YVIYDIPRALPLKVAEKIKKSLRETDFCHNASIWIGTES 689
Query: 761 SQVFKYPTREVVKACELIVDKKTSQKKEVECKVRDNAMSIEFFQALLSKSQKQRGTVESI 820
+V P ++ + DK+ E +R + +FF + S++++ + + +
Sbjct: 690 RKVLNIPLNDLRRMFHQQWDKQRETISVAENDLRICQKARKFFGEIESRTREAKKKIMMV 749
Query: 821 NLE 823
LE
Sbjct: 750 QLE 752
>CE02898 [O] KOG0448 Mitofusin 1 GTPase involved in mitochondrila biogenesis
Length = 774
Score = 52.4 bits (124), Expect = 3e-06
Identities = 49/202 (24%), Positives = 93/202 (45%), Gaps = 17/202 (8%)
Query: 148 DHLINLQTRVQDTSSKVFITGDLNSGKSTLCNAFLRKKVLPEDQLPCTNVFCEILEAREN 207
D + + Q + KV G ++GKST NA L +KVLP+ T F ++ +
Sbjct: 87 DSIKTIMDTFQRDNMKVVFFGRTSNGKSTTINAMLHEKVLPQGMGHTTCCFLQVEGSEGE 146
Query: 208 GNMERVHAIPKTVATNVKDASVLYDMRDRSTYEDYTLDKLDQLVYDNDHYILLKIYIKDD 267
++ P+ + D +L + + E+ L + Q D+ LLK++
Sbjct: 147 VGHLQLDDNPQKI-----DMKMLGKIGHALSDENSDLPAMGQ---DS----LLKVFHPKK 194
Query: 268 KRPVDSSLLRNGTADISLIDSPGLNMDSVKTTEVMSRQEEIDLVVFVVNAENQLTLSARE 327
+ LL+N D+ ++DSPG+++ + + + D+ V V NAE+ LT + +
Sbjct: 195 SESGECRLLQN---DVVILDSPGVDLSPEFDSWIDKHCLDADVFVLVSNAESTLTQAEKN 251
Query: 328 FITMASRE--KKLMFFVINKFD 347
F +++ K +F + N++D
Sbjct: 252 FFLRVAKKLSKPNVFILNNRWD 273
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.132 0.364
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,452,764
Number of Sequences: 60738
Number of extensions: 1748684
Number of successful extensions: 5649
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 5636
Number of HSP's gapped (non-prelim): 4
length of query: 825
length of database: 30,389,216
effective HSP length: 115
effective length of query: 710
effective length of database: 23,404,346
effective search space: 16617085660
effective search space used: 16617085660
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)