ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV6294 good O KOG0909 Posttranslational modification, protein turnover, chaperones Peptide:N-glycanase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV6294 2159885 2158827 -353
(353 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPL096w [O] KOG0909 Peptide:N-glycanase 395 e-110
SPBC1709.14 [O] KOG0909 Peptide:N-glycanase 222 6e-58
At5g49570 [O] KOG0909 Peptide:N-glycanase 189 4e-48
Hs21314690 [O] KOG0909 Peptide:N-glycanase 177 2e-44
CE23786_2 [O] KOG0909 Peptide:N-glycanase 159 4e-39
7302197 [O] KOG0909 Peptide:N-glycanase 128 1e-29
>YPL096w [O] KOG0909 Peptide:N-glycanase
Length = 363
Score = 395 bits (1015), Expect = e-110
Identities = 189/351 (53%), Positives = 238/351 (66%), Gaps = 10/351 (2%)
Query: 10 SVSKRFLELYRSKILSQWRGTNGSSEEEKRFNSLIQQNGFARQIFGLYKSLCFRYENDAW 69
S++K L Y+ ILS+++ + E RF +L+ N FA+ + G + LC Y+N +W
Sbjct: 14 SIAKMLLIKYKDFILSKFK--KAAPVENIRFQNLVHTNQFAQGVLGQSQHLCTVYDNPSW 71
Query: 70 YSVVXXXXXXXXXYSNVDKAVQDNNASDIE--YQDYLVKELLRYFKRDFFSWCNKPNCSK 127
+S+V Y NVDK + ++ E Y DYLVKELLRYFK+DFF WCNKP+C+
Sbjct: 72 HSIVLETLDLDLIYKNVDKEFAKDGHAEGENIYTDYLVKELLRYFKQDFFKWCNKPDCNH 131
Query: 128 CG--TDEHLEWVGTDRANSEEAKYQCGNVEVYRCTLTGDITRFPRYNDPIKLLQTRTGRC 185
CG T E++ +G+ N EE+K+ CG VE+Y+C G+ITRFPRYNDPIKLL+TR GRC
Sbjct: 132 CGQNTSENMTPLGSQGPNGEESKFNCGTVEIYKCNRCGNITRFPRYNDPIKLLETRKGRC 191
Query: 186 GEWCNVFTLILKSFGLNARYIWNKEDHVWCEYYSPNLKRWVHLDCCEQSFDEPHIYSKNW 245
GEWCN+FTLILKSFGL+ RY+WN+EDHVWCEY+S L RWVH+D CEQSFD+P+IYS NW
Sbjct: 192 GEWCNLFTLILKSFGLDVRYVWNREDHVWCEYFSNFLNRWVHVDSCEQSFDQPYIYSINW 251
Query: 246 NKKMSYVLAFSNDIVADVSGRYILQNNLPRNAISENELNFMCTYLTKTLRKDFNDDQIYA 305
NKKMSY +AF D V DVS RYILQN LPR+ I E +L F+C ++TK LR NDD+IY
Sbjct: 252 NKKMSYCIAFGKDGVVDVSKRYILQNELPRDQIKEEDLKFLCQFITKRLRYSLNDDEIYQ 311
Query: 306 LACRDEQERLSL----EXXXXXXXXXXXXXXXXXXRESGSTAWKQQRGEDG 352
LACRDEQE++ L RESGS WK QRGEDG
Sbjct: 312 LACRDEQEQIELIRGKTQETKSESVSAASKSSNRGRESGSADWKAQRGEDG 362
>SPBC1709.14 [O] KOG0909 Peptide:N-glycanase
Length = 333
Score = 222 bits (566), Expect = 6e-58
Identities = 118/277 (42%), Positives = 156/277 (55%), Gaps = 14/277 (5%)
Query: 86 VDKAVQDNNASDIE--------YQDYLVKELLRYFKRDFFSWCNKPNCSKCGTDEHLEWV 137
+DK QD AS++E YQDY+++ LL++FKR+FF W N+P C KCG + H+
Sbjct: 60 LDKLFQD--ASELEKEGDGSWGYQDYVIQALLKWFKREFFVWVNQPPCEKCGGETHM--T 115
Query: 138 GTDRANSEEAKYQCGNVEVYRCTLTGDITRFPRYNDPIKLLQTRTGRCGEWCNVFTLILK 197
G N EE NVE+Y+C + G RFPRYN LL +R GRCGEW N FT + +
Sbjct: 116 GNGPPNEEEKWNGVRNVELYQCNVCGHNQRFPRYNRIRALLDSRKGRCGEWANCFTFLCR 175
Query: 198 SFGLNARYIWNKEDHVWCEYYSPNLKRWVHLDCCEQSFDEPHIYSKNWNKKMSYVLAFSN 257
+ G AR+IWN EDHVW E YS +RWVH+D E+SFDEP IY + W KKMSY L F
Sbjct: 176 ALGSRARWIWNAEDHVWTEVYSNKQQRWVHVDSGEESFDEPLIYEQGWGKKMSYCLGFGI 235
Query: 258 DIVADVSGRYIL--QNNLPRNAISENELNFMCTYLTKTLRKDFNDDQIYALACRDEQERL 315
D V DVS RYI +N LPR+ E+ L + R D + AL D++E+
Sbjct: 236 DSVRDVSHRYIRHPENGLPRDRCPESVLQQALHEINIEFRSRLTDSERKALEEEDKREKD 295
Query: 316 SLEXXXXXXXXXXXXXXXXXXRESGSTAWKQQRGEDG 352
L+ R++G+ WK++RGE G
Sbjct: 296 ELDGYMRPVSQATPTNTDLPARQTGNVEWKEKRGEAG 332
>At5g49570 [O] KOG0909 Peptide:N-glycanase
Length = 721
Score = 189 bits (481), Expect = 4e-48
Identities = 107/274 (39%), Positives = 148/274 (53%), Gaps = 14/274 (5%)
Query: 86 VDKAVQDNNASDIEYQDY-LVKELLRYFKRDFFSWCNKPNCSKCGTDEHLEWVGTDRAN- 143
V A + N E +DY + +LL +FK+ F W N+P C CG + +G N
Sbjct: 154 VSLAKEGNFEPSKEERDYAFLLQLLFWFKKSF-RWVNEPPCDFCGN----KTIGQGMGNP 208
Query: 144 -SEEAKYQCGNVEVYRCTLTGDITRFPRYNDPIKLLQTRTGRCGEWCNVFTLILKSFGLN 202
+ E Y VE+YRCT+ TRFPRYNDP+KL++T+ GRCGEW N FTL ++FG +
Sbjct: 209 LTSELAYGANRVEIYRCTMCPTTTRFPRYNDPLKLVETKKGRCGEWANCFTLYCRTFGYD 268
Query: 203 ARYIWNKEDHVWCEYYSPNLKRWVHLDCCEQSFDEPHIYSKNWNKKMSYVLAFSNDIVAD 262
+R I + DHVW E YS +LKRW+HLD CE +D+P +Y K WNKK++YV+A S D V D
Sbjct: 269 SRLIMDFTDHVWTECYSHSLKRWIHLDPCEGVYDKPMLYEKGWNKKLNYVIAISKDGVCD 328
Query: 263 VSGRYILQNN---LPRNAISENELNFMCTYLTKTLRKDFNDDQIYALACRDEQERLSLEX 319
V+ RY + + R +E+ L LT+ R+ + + L RD E+ LE
Sbjct: 329 VTKRYTKKWHEVLSRRTLTTESSLQDGLRTLTRERRRSLMFESLSKLELRDRNEQEELE- 387
Query: 320 XXXXXXXXXXXXXXXXXRESGSTAWKQQRGEDGS 353
R+SG W+ R E GS
Sbjct: 388 --RNLHSADNASVSLPGRQSGDREWRIMRSEFGS 419
>Hs21314690 [O] KOG0909 Peptide:N-glycanase
Length = 654
Score = 177 bits (449), Expect = 2e-44
Identities = 100/262 (38%), Positives = 136/262 (51%), Gaps = 16/262 (6%)
Query: 97 DIEYQDYLVKELLRYFKRDFFSWCNKPNCSKCGTDEHLEWVGTDRA---NSEEAKYQCGN 153
+I +D+L+ ELL +FK +FF W N CSKCG DR+ + +E K+
Sbjct: 222 NISDEDFLLLELLHWFKEEFFHWVNNVLCSKCGGQTR----SRDRSLLPSDDELKWGAKE 277
Query: 154 VEVYRCTLTGDITRFPRYNDPIKLLQTRTGRCGEWCNVFTLILKSFGLNARYIWNKEDHV 213
VE + C RFPRYN+P KLL+TR GRCGEW N FTL ++ G ARY+W+ DHV
Sbjct: 278 VEDHYCDACQFSNRFPRYNNPEKLLETRCGRCGEWANCFTLCCRAVGFEARYVWDYTDHV 337
Query: 214 WCEYYSPNLKRWVHLDCCEQSFDEPHIYSKNWNKKMSYVLAFSNDIVADVSGRYILQNN- 272
W E YSP+ +RW+H D CE D+P +Y W KK+SYV+AFS D V DV+ RY ++
Sbjct: 338 WTEVYSPSQQRWLHCDACEDVCDKPLLYEIGWGKKLSYVIAFSKDEVVDVTWRYSCKHEE 397
Query: 273 --LPRNAISENELNFMCTYLTKTLRKDFNDDQIYALACRDEQERLSLEXXXXXXXXXXXX 330
R + E L L K + ++++ R E + +
Sbjct: 398 VIARRTKVKEALLRDTINGLNKQRQLFLSENR------RKELLQRIIVELVEFISPKTPK 451
Query: 331 XXXXXXRESGSTAWKQQRGEDG 352
R SGS AW+ RGE G
Sbjct: 452 PGELGGRISGSVAWRVARGEMG 473
>CE23786_2 [O] KOG0909 Peptide:N-glycanase
Length = 500
Score = 159 bits (403), Expect = 4e-39
Identities = 86/256 (33%), Positives = 128/256 (49%), Gaps = 9/256 (3%)
Query: 104 LVKELLRYFKRDFFSWCNKPNCSKCGTDEHLEWVGTDRANSEEAKYQCGNVEVYRCTLTG 163
++K+LL +FK FF+W ++P C KC + + E+ + VEVY C
Sbjct: 64 ILKDLLHWFKTQFFTWFDRPTCPKCTLKCSTDGLQGTPTREEQKEGGASRVEVYICDGCN 123
Query: 164 DITRFPRYNDPIKLLQTRTGRCGEWCNVFTLILKSFGLNARYIWNKEDHVWCEYYSPNLK 223
RFPRYN+P KLLQTRTGRCGEW N F L+L + L +R+I++ DHVW E Y +
Sbjct: 124 TEMRFPRYNNPAKLLQTRTGRCGEWANCFGLLLAALNLESRFIYDTTDHVWNEVYLLAEQ 183
Query: 224 RWVHLDCCEQSFDEPHIYSKNWNKKMSYVLAFSNDIVADVSGRYI-------LQNNLPRN 276
RW H+D CE + D P +Y++ W K + Y + + +D V DV+ RYI Q N R
Sbjct: 184 RWCHVDPCENTMDRPLLYTRGWGKTLGYCIGYGSDHVVDVTWRYIWDSKKLVTQRNEVRQ 243
Query: 277 AISENELNFMCTYLTKTLRKDFNDDQIYALACRDEQERLSLEXXXXXXXXXXXXXXXXXX 336
+ EN L+ + + + + + C E + +
Sbjct: 244 PVFENFLSKLNSRQAEGQTEPRKRELAVRRVC--ELMEMMAQEAKNHKIGWEKIGDDLGG 301
Query: 337 RESGSTAWKQQRGEDG 352
R +GS W+++RGE G
Sbjct: 302 RITGSEEWRRERGELG 317
>7302197 [O] KOG0909 Peptide:N-glycanase
Length = 591
Score = 128 bits (322), Expect = 1e-29
Identities = 78/253 (30%), Positives = 119/253 (46%), Gaps = 19/253 (7%)
Query: 101 QDYLVKELLRYFKRDFFSWCNKPNCSKCGTDEHLEWVGTDRANSEEAKYQCGNVEVYRCT 160
+D L+ EL+ +F FF W N C CG++E R E + G++ V
Sbjct: 222 RDLLLVELVNWFNTQFFQWVNNIPCRVCGSEE-------SRLRRTERE---GDIRVEVTV 271
Query: 161 LTGDITRFPRYNDPIKLLQTRTGRCGEWCNVFTLILKSFGLNARYIWNKEDHVWCEYYSP 220
G ++F RYND +LL +R GRCGE+ N FT + ++ +AR + + DHVW E YS
Sbjct: 272 CCGQESKFYRYNDISQLLVSRKGRCGEYANCFTFLCRALDYDARIVHSHFDHVWTEVYSE 331
Query: 221 NLKRWVHLDCCEQSFDEPHIYSKNWNKKMSYVLAFSNDIVADVSGRYILQNNL---PRNA 277
RW+H+D E D P +Y W + + Y+LA+S D + DV+ RY + R
Sbjct: 332 AQMRWLHVDPSENVIDSPLMYQHGWKRHIDYILAYSRDDIQDVTWRYTNDHQKILHLRKL 391
Query: 278 ISENELNFMCTYLTKTLRKDFNDDQIYALACRDEQERLSLEXXXXXXXXXXXXXXXXXXR 337
E E+ + R++ D+ L+ R+ E + L R
Sbjct: 392 CGEKEMVQTLDAIRAKRRQNCTADRKLFLSQRNMYEVIGL------TLERKPTENELKGR 445
Query: 338 ESGSTAWKQQRGE 350
SGS +W+Q +GE
Sbjct: 446 SSGSLSWRQSQGE 458
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.135 0.428
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,231,352
Number of Sequences: 60738
Number of extensions: 899957
Number of successful extensions: 1881
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1862
Number of HSP's gapped (non-prelim): 8
length of query: 353
length of database: 30,389,216
effective HSP length: 107
effective length of query: 246
effective length of database: 23,890,250
effective search space: 5877001500
effective search space used: 5877001500
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)