ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV6316 good BK KOG3949 Chromatin structure and dynamics RNA polymerase II elongator complex, subunit ELP4
r_klactV6316 good BK KOG3949 Transcription RNA polymerase II elongator complex, subunit ELP4
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV6316 2166614 2165181 -478
(478 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPL101w [BK] KOG3949 RNA polymerase II elongator complex subunit... 427 e-119
SPCC11E10.06c [BK] KOG3949 RNA polymerase II elongator complex s... 73 8e-13
HsM9506685 [BK] KOG3949 RNA polymerase II elongator complex subu... 62 2e-09
Hs21361629_1 [BK] KOG3949 RNA polymerase II elongator complex su... 60 7e-09
>YPL101w [BK] KOG3949 RNA polymerase II elongator complex subunit ELP4
Length = 456
Score = 427 bits (1099), Expect = e-119
Identities = 228/447 (51%), Positives = 304/447 (68%), Gaps = 31/447 (6%)
Query: 1 MSFRKRGEVLSD-GNAARRNIVPGGMIPRDMPSRNNAQRPSGAISRGPMGGAAPGRVVPN 59
MSFRKRGE+L+D G+ R ++ G P R +R APG V
Sbjct: 1 MSFRKRGEILNDRGSGLRGPLLRGPPRTSSTPLRTGNRR-------------APGNVP-- 45
Query: 60 IGNVHRDLKGLHISGRTAQAIPQAEASSGFSAVHPGIRPSPAXXXXXXXXGCTDLDKLMG 119
+ + LK L+I+ +++ G + H G+RPSPA G DLD ++G
Sbjct: 46 LSDTTARLKKLNIAD-------ESKTKMGLDSSHVGVRPSPATSQPTTSTGSADLDSILG 98
Query: 120 HSGFPLGCSLLIEEESTTDFNSILTKVYASQGIVHNRIEGSHGFK--DGNTHLIVLSLNH 177
H G PLG S+L+EE+STT+F+SIL K++A+QGIVHNRI S K +G+TH+IVLSLN
Sbjct: 99 HMGLPLGNSVLVEEQSTTEFHSILGKLFAAQGIVHNRISDSSADKTRNGDTHVIVLSLNQ 158
Query: 178 HLTKELPGVYKGSRKDIKKSKIAQEQSKVTVSNLLESQSKSNTSAQRNKDLKIAWRYGMN 237
KELPG+YKGSRK +KK+ I++E+SKVTV NL E+Q ++ R KDLKIAW+Y +
Sbjct: 159 MFAKELPGIYKGSRKQMKKNLISEEESKVTVQNLNETQ---RSTPSRYKDLKIAWKYKLA 215
Query: 238 DDSAKSESSRE-VDQSETYPDYNHQFDITSTLIPAPTSNEISFISPLLPAASVLSQMESI 296
D+ R+ + Q+ Y DYNHQFDIT+ L+PAP ++E++FI+P P +++LSQ+E
Sbjct: 216 DEKRLGSPDRDDIQQNSEYKDYNHQFDITTRLMPAPIASELTFIAPTQPVSTILSQIEQT 275
Query: 297 IKRHNSKLIRILLPNLLHPALYPPKMTQLHELIPLLHGLRSLLKKYKERCVMLSTISTSL 356
IKR++ KLIRI++P+LLHPA+YPPKM + E+I L+HG+RSL+KKY ER V+ ++IS +
Sbjct: 276 IKRNDKKLIRIVIPSLLHPAMYPPKMFESSEIIGLMHGVRSLVKKYYERVVLFASISIDI 335
Query: 357 YHNQPLFTAQIEQLFDGVIVLEPFGQEMLQFLERAYKSQPNKIQHGLVHIKKLPVFSERG 416
P + +FD VI LEPF QEM +FLER YKSQP KIQHGLVHI KLPVF++RG
Sbjct: 336 I--TPPLLVLLRNMFDSVINLEPFNQEMTEFLERVYKSQPGKIQHGLVHILKLPVFTDRG 393
Query: 417 EMHVSKSEWAFKNGRKRFEIEEWGIPV 443
EM V KSEWAFKNGRK+FEIE+WGIPV
Sbjct: 394 EMRVLKSEWAFKNGRKKFEIEQWGIPV 420
>SPCC11E10.06c [BK] KOG3949 RNA polymerase II elongator complex subunit ELP4
Length = 361
Score = 73.2 bits (178), Expect = 8e-13
Identities = 88/359 (24%), Positives = 145/359 (39%), Gaps = 53/359 (14%)
Query: 95 GIRPSPAXXXXXXXXGCTDLDKLMGHSGFPLGCSLLIEEESTTDFNSILTKVYASQGIVH 154
G+R S G + D + G P+ SLL+ EE + D+ S+L K +A++G+
Sbjct: 19 GVRLSSKDARWITSSGSSSFDYYLS-GGIPMK-SLLVIEEDSMDYASVLLKFFAAEGLKQ 76
Query: 155 NRIEGSHGFKDGNTHLIVL--SLNHHLTKELPGVYKGSRKDIKKSKIAQEQSKVTVSNLL 212
+ H+I L S+ ++LPG S + K A E +
Sbjct: 77 D-------------HVIWLGPSIGEMWFRQLPG---DSDRPNKNENSAGEDNH------- 113
Query: 213 ESQSKSNTSAQRNKDLKIAWRYGMNDDSAKSESSREVDQSETYPDYNHQFDITSTLIPAP 272
S N +R +KIAWRY + + +D P Y H FD++ LI
Sbjct: 114 SSPPSKNPQQER---MKIAWRY----EQVSKTKAPTLDMIP--PGYTHSFDLSKNLIVKS 164
Query: 273 TSNEISFISPLLPAASVLSQMESIIKRHNSKLI-----RILLPNLLHPALYPPKMTQLHE 327
PL ++ + + + R S L R++LP++L PA Y + T
Sbjct: 165 DMKYAVSPFPLETGSNPYAPVIESLTRFLSTLTPGTVCRLVLPSILSPAFYSIRATHPQH 224
Query: 328 LIPLLHGLRSLLK-KYKERCVMLSTISTSLYHNQPLFTAQIEQLFDGVIVLEPFGQEMLQ 386
I +H L SL+K + + ++ ++L+ +E L V L PF ++
Sbjct: 225 FIRFIHTLSSLIKCTTSVHLICMCSVPSTLFSRDCEQIFWLENLASAVFSLHPF---PVK 281
Query: 387 FLERAYKSQPNKIQHGLVHIKKLPV---FSERGEMHVSKSEWAFKNGRKRFEIEEWGIP 442
+QP GL I KLP+ F+ + + +F ++RF IE W +P
Sbjct: 282 ETVNGLVTQP----LGLFRIHKLPLALPFTNHANSN-EAGDLSFTVSKRRFTIEPWVLP 335
>HsM9506685 [BK] KOG3949 RNA polymerase II elongator complex subunit ELP4
Length = 424
Score = 62.0 bits (149), Expect = 2e-09
Identities = 89/375 (23%), Positives = 151/375 (39%), Gaps = 66/375 (17%)
Query: 95 GIRPSPAXXXXXXXXGCTDLDKLMGHSGFPLGCSLLIEEESTTDFNSILTKVYASQGIVH 154
G RPS G LD+L+G G +G LLIEE+ ++ +L K + ++GIV+
Sbjct: 51 GTRPSVRNGQLLVSTGLPALDQLLG-GGLAVGTVLLIEEDKYNIYSPLLFKYFLAEGIVN 109
Query: 155 NRIEGSHGFKDGNTHLIVLSLNH--HLTKELPGVYKGSRKDIKKSKIAQEQSKVTVSNLL 212
G+T L+ + ++ +ELP + K +E
Sbjct: 110 -----------GHTLLVASAKEDPANILQELPA-------PLLDDKCKKE---------F 142
Query: 213 ESQSKSNTSAQRNKDLKIAWRYGMNDD---------------SAKSESSREVDQSETYPD 257
+ ++ + + N +KIAWRY + A +E+ ++ +
Sbjct: 143 DEDVYNHKTPESNIKMKIAWRYQLLPKMEIGPVSSSRFGHYYDASKRMPQELIEASNWHG 202
Query: 258 YNHQFDITSTLIPAPTSNEISFISPLLPAASVLSQME---SIIKRHNSKLIRILLPNLLH 314
+ I+STL P S + L +++ + S ++ ++RI + NL
Sbjct: 203 FFLPEKISSTLKVEPCSLTPGYTKLLQFIQNIIYEEGFDGSNPQKKQRNILRIGIQNLGS 262
Query: 315 PA-----LYPPKMTQLHELIPLLHGLRSLLKKYKERCVMLSTISTSLYHNQPLFTAQIEQ 369
P H L L+ LR LL+ C++ T+ T L N+ + A++
Sbjct: 263 PLWGDDICCAENGGNSHSLTKFLYVLRGLLRTSLSACII--TMPTHLIQNKAII-ARVTT 319
Query: 370 LFDGVIVLEPF-GQEMLQFLERAYKSQP-NKIQHGLVHIKKLPVFSERGEMHVSKSEWAF 427
L D V+ LE F G E ++ P K HGL+HI+++P + + AF
Sbjct: 320 LSDVVVGLESFIGSER--------ETNPLYKDYHGLIHIRQIPRLNNLICDESDVKDLAF 371
Query: 428 KNGRKRFEIEEWGIP 442
K RK F IE +P
Sbjct: 372 KLKRKLFTIERLHLP 386
>Hs21361629_1 [BK] KOG3949 RNA polymerase II elongator complex subunit ELP4
Length = 381
Score = 60.1 bits (144), Expect = 7e-09
Identities = 88/370 (23%), Positives = 149/370 (39%), Gaps = 66/370 (17%)
Query: 95 GIRPSPAXXXXXXXXGCTDLDKLMGHSGFPLGCSLLIEEESTTDFNSILTKVYASQGIVH 154
G RPS G LD+L+G G +G LLIEE+ ++ +L K + ++GIV+
Sbjct: 51 GTRPSVRNGQLLVSTGLPALDQLLG-GGLAVGTVLLIEEDKYNIYSPLLFKYFLAEGIVN 109
Query: 155 NRIEGSHGFKDGNTHLIVLSLNH--HLTKELPGVYKGSRKDIKKSKIAQEQSKVTVSNLL 212
G+T L+ + ++ +ELP + K +E
Sbjct: 110 -----------GHTLLVASAKEDPANILQELPA-------PLLDDKCKKE---------F 142
Query: 213 ESQSKSNTSAQRNKDLKIAWRYGMNDD---------------SAKSESSREVDQSETYPD 257
+ ++ + + N +KIAWRY + A +E+ ++ +
Sbjct: 143 DEDVYNHKTPESNIKMKIAWRYQLLPKMEIGPVSSSRFGHYYDASKRMPQELIEASNWHG 202
Query: 258 YNHQFDITSTLIPAPTSNEISFISPLLPAASVLSQME---SIIKRHNSKLIRILLPNLLH 314
+ I+STL P S + L +++ + S ++ ++RI + NL
Sbjct: 203 FFLPEKISSTLKVEPCSLTPGYTKLLQFIQNIIYEEGFDGSNPQKKQRNILRIGIQNLGS 262
Query: 315 PA-----LYPPKMTQLHELIPLLHGLRSLLKKYKERCVMLSTISTSLYHNQPLFTAQIEQ 369
P H L L+ LR LL+ C++ T+ T L N+ + A++
Sbjct: 263 PLWGDDICCAENGGNSHSLTKFLYVLRGLLRTSLSACII--TMPTHLIQNKAII-ARVTT 319
Query: 370 LFDGVIVLEPF-GQEMLQFLERAYKSQP-NKIQHGLVHIKKLPVFSERGEMHVSKSEWAF 427
L D V+ LE F G E ++ P K HGL+HI+++P + + AF
Sbjct: 320 LSDVVVGLESFIGSER--------ETNPLYKDYHGLIHIRQIPRLNNLICDESDVKDLAF 371
Query: 428 KNGRKRFEIE 437
K RK F IE
Sbjct: 372 KLKRKLFTIE 381
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.133 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,194,185
Number of Sequences: 60738
Number of extensions: 1187010
Number of successful extensions: 3417
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 3406
Number of HSP's gapped (non-prelim): 6
length of query: 478
length of database: 30,389,216
effective HSP length: 110
effective length of query: 368
effective length of database: 23,708,036
effective search space: 8724557248
effective search space used: 8724557248
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)