ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV6335 good R KOG2382 General function prediction only Predicted alpha/beta hydrolase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV6335 2171139 2172242 368
(368 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGR031w [R] KOG2382 Predicted alpha/beta hydrolase 127 3e-29
SPAC22H12.03 [R] KOG2382 Predicted alpha/beta hydrolase 119 7e-27
7290805 [R] KOG2382 Predicted alpha/beta hydrolase 105 1e-22
YGR015c [R] KOG2382 Predicted alpha/beta hydrolase 104 2e-22
CE18906 [R] KOG2382 Predicted alpha/beta hydrolase 96 1e-19
7299497 [R] KOG2382 Predicted alpha/beta hydrolase 65 1e-10
CE17746 [R] KOG2382 Predicted alpha/beta hydrolase 52 2e-06
>YGR031w [R] KOG2382 Predicted alpha/beta hydrolase
Length = 342
Score = 127 bits (318), Expect = 3e-29
Identities = 95/309 (30%), Positives = 156/309 (49%), Gaps = 31/309 (10%)
Query: 37 LPHKEV-IDMAFDLHLPERSVIGKLPYHSPEPIIFFHGLLGSKRNYKHDCKKLATALQTP 95
L K+V +D+++D+ + G P PII HGL G+K N + + L L
Sbjct: 46 LQQKQVPLDLSYDIIKRDAVKTGDEGKPRP-PIIILHGLFGNKLNNRSIGRNLNKKLGRD 104
Query: 96 VYTVDVRNHGSSEHALPFNYGTLVNDLVHFIHQHKL---GKVNIIGYSLGAKVGMLACLK 152
VY +D+RNHGSS H+ NY + D+ HFI +H+L G IIG+S+G KV M+ LK
Sbjct: 105 VYLLDLRNHGSSPHSSVHNYEVMSEDVKHFITKHELNTNGGPIIIGHSMGGKVAMMLVLK 164
Query: 153 HPELFSAACIIDNAP---EPQPHIKAFLSTLIKSCVKLLDQGKV-----RVDDKL-WRHK 203
+P+L S I+NAP P ++ L++ + D+GK + D+ L R
Sbjct: 165 NPQLCSMLVCIENAPVSLRPNAEFVEYIKALMEI---VNDKGKTIRTLKQADEHLAERIG 221
Query: 204 ASESLKRYLPNAGVRNYVLNNIVHNPRVVEYRSPVINYDDGLIHFKNPVRHMLDCGVKDV 263
+E ++R+L A + + N+ + E R P+ D ++ ++
Sbjct: 222 GNELVRRFLLTALKKVKMDNSSSVSSYTFEERIPLATLKDAIVK-------------GEI 268
Query: 264 ADWPTEEVKDKKFLGPVNFIRATKSAFINPDSLKAINSFFPFNNIHEINATHFVLNERPQ 323
A WP + ++ ++ P FIRAT+S ++ + L I +FFP +I+A H+V E+P
Sbjct: 269 AAWPLDPARE-RWTRPALFIRATQSHYVVDEYLPIIGAFFPRFETRDIDAGHWVNAEKPG 327
Query: 324 EYLRAVIDF 332
E +++DF
Sbjct: 328 ECAESIVDF 336
>SPAC22H12.03 [R] KOG2382 Predicted alpha/beta hydrolase
Length = 270
Score = 119 bits (298), Expect = 7e-27
Identities = 72/269 (26%), Positives = 130/269 (47%), Gaps = 26/269 (9%)
Query: 67 PIIFFHGLLGSKRNYKHDCKKLATALQTPVYTVDVRNHGSSEHALPFNYGTLVNDLVHFI 126
P++ FHGLLGSKRN++ KK + L +Y +D R HG S P +Y + D F+
Sbjct: 22 PVLIFHGLLGSKRNWRSLAKKFSCKLDRDIYAIDQRCHGDSPCVAPLSYSAMALDAFQFM 81
Query: 127 HQHKLGKVNIIGYSLGAKVGMLACLKHPELFSAACIIDNAPEPQPHIKAFLSTLIKSCVK 186
HKL K +IIG+S+GAK M+ LK P+ ++DN+P Q + + + ++
Sbjct: 82 KDHKLDKASIIGHSMGAKTAMVTALKWPDKVEKLVVVDNSPWYQDLPRDY-GAYFRKMIQ 140
Query: 187 LLDQGKVRVDDKLWRHKASESLKRYLPNAGVRNYVLNNI---VHNPRVVEYRSPVINYDD 243
+ + + + A + + + VR+++L+N+ +N ++R P+
Sbjct: 141 IDEANITKYSE------ADKMMSTVEKDILVRSFLLSNLKKDSNNSNTFKFRVPI----- 189
Query: 244 GLIHFKNPVRHMLDCGVKDVADWPTEEVKDKKFLGPVNFIRATKSAFINPDSLKAINSFF 303
++ +K + +P + D + P IRA K+ FI +L FF
Sbjct: 190 ----------ELISKSLKTIEGFPA-SLNDLVYDSPTLVIRALKAPFIPDSALPVFKKFF 238
Query: 304 PFNNIHEINATHFVLNERPQEYLRAVIDF 332
P + ++ H+V E+P+E+ ++I+F
Sbjct: 239 PKYELVSLDCGHWVHFEKPKEFSESIINF 267
>7290805 [R] KOG2382 Predicted alpha/beta hydrolase
Length = 308
Score = 105 bits (262), Expect = 1e-22
Identities = 78/301 (25%), Positives = 135/301 (43%), Gaps = 37/301 (12%)
Query: 43 IDMAFDLHLPERSVIGKLPYHSPEPIIFFHGLLGSKRNYKHDCKKLATALQTPVYTVDVR 102
++++FD S G+ P SP P++ +HGL GSK+N++ K L + VY +DVR
Sbjct: 37 VELSFD------SYTGENPETSP-PLLTYHGLFGSKQNWRGISKALVRKVSRKVYAIDVR 89
Query: 103 NHGSSEHALPFNYGTLVNDLVHFIHQHKLGKVNIIGYSLGAKVGMLACLKHPELFSAACI 162
NHG S H+ N + DL F+ Q +G+S+G + M K+PEL +
Sbjct: 90 NHGESPHSSVHNSKAMSEDLRLFMEQRSHPNAACMGHSMGGRSMMYFARKYPELVERLIV 149
Query: 163 IDNAPEPQPHIKAFLSTLIKSCVKL-------LDQGKVRVDDKLWRHKASESLKRYLPNA 215
+D +P P ++ + + V L + +G+ +KL + E++
Sbjct: 150 VDISPISVPRSTGEMTEIFDAMVSLDLSPSMSMSEGRKIAREKLLKATEDETV------- 202
Query: 216 GVRNYVLNNIVHNPRVVEYRSPVINYDDGLIHFKNPVRHMLDCGVKDVADWPTEEVKDKK 275
++++ N+ NP D G + H+L + + + +
Sbjct: 203 ---DFIMLNLRKNP------------DTGAFSWACNA-HVLREFLTRFDKYQSNLEELPP 246
Query: 276 FLGPVNFIRATKSAFINPDSLKAINSFFPFNNIHEINATHFVLNERPQEYLRAVIDFFKV 335
+ GP FI T+S ++ + I FP + IH ++A H V E+PQE+L V +F
Sbjct: 247 YTGPTTFICGTRSPYMRREQWPQIQKMFPNSEIHWLDAGHLVHFEKPQEFLTIVSEFLNR 306
Query: 336 T 336
T
Sbjct: 307 T 307
>YGR015c [R] KOG2382 Predicted alpha/beta hydrolase
Length = 328
Score = 104 bits (260), Expect = 2e-22
Identities = 93/323 (28%), Positives = 147/323 (44%), Gaps = 38/323 (11%)
Query: 37 LPHKEVIDMAFDLHLPERSVIGKLPYHSPEPIIFFHGLLGSKRNYKHDCKKLATALQTPV 96
LP+K ++D++F V + + II HGLLGS + K L+ L +
Sbjct: 10 LPYKIIVDLSFHRTRLPSDVSSLIKFEQRPAIINIHGLLGSHVMFHSLNKLLSRKLDADI 69
Query: 97 YTVDVRNHGSSEHALPFNYGTLVNDLVHFIHQHKLG---KVNIIGYSLGAKVGMLACLKH 153
++VDVRNHG S A+P++Y TL NDL++FI H +G + ++G+S+G K+ +L L +
Sbjct: 70 FSVDVRNHGISPKAIPYDYTTLTNDLIYFIETH-IGLERPIYLLGFSMGGKIALLTTL-Y 127
Query: 154 PELFSAACI-IDNAPEPQPHIKAFLSTLIKSCVKLLDQG-KVRVDDKLWRHKASESLKRY 211
+ CI ID P P + + ++++ + K+ W+ K E K
Sbjct: 128 KNINIRKCISIDLPPYETPELDPMILQNYDLIMRIIRRDVKILRGSPSWQKKVLELFKSL 187
Query: 212 LPN-----AGVRNYVLN-------NIVHNPRV---VEYRSPVINYDDGLIHFKNPVRHML 256
N V Y N N VH ++ + P INY L N +L
Sbjct: 188 ECNKRKCGGAVALYFANGFLSVKSNNVHQAQLHYEQQQHDPYINYSMPLSSMPN----LL 243
Query: 257 DCGVKDVADWPTEEVKDKKF-----LGPVNFIRATKSAFINPD-SLKAINSFFPFNNIHE 310
D +V WP + F V F++ +S FIN D SL N FP ++ E
Sbjct: 244 D----EVKKWPDLSNQRDFFQKGTASRKVLFMKGLQSNFINNDYSLLRYN--FPCADVRE 297
Query: 311 INATHFVLNERPQEYLRAVIDFF 333
N H +L E P++ + +++FF
Sbjct: 298 FNTGHNLLLENPEDSFKCILNFF 320
>CE18906 [R] KOG2382 Predicted alpha/beta hydrolase
Length = 299
Score = 95.5 bits (236), Expect = 1e-19
Identities = 77/285 (27%), Positives = 129/285 (45%), Gaps = 35/285 (12%)
Query: 62 YHSPE-----PIIFFHGLLGSKRNYKHDCKKLATALQTPVYTVDVRNHGSSEHALPFNYG 116
Y SPE P++ HGL G K+N+ K L L+ PVY VDVRNHGSS H +Y
Sbjct: 37 YSSPELDRNSPLVIVHGLFGQKQNWNSVGKALHKKLEAPVYAVDVRNHGSSPHTETMSYT 96
Query: 117 TLVNDLVHFI----HQHKLGKVNIIGYSLGAKVGMLACL--KHPELFSAACIIDNAPEPQ 170
+ DLV FI + K +VN++G+S+G K+ M + K + + D +P+
Sbjct: 97 EMAEDLVLFIDKVKEETKKTRVNLLGHSMGGKIVMRLAIDSKWSDRIEKLIVEDVSPKGY 156
Query: 171 PHIKAFLSTLIKSCVKLLDQGKVRVDDKLWRHKASESLKRYLPNAGVRNYVLNNIVHNPR 230
LIK+ VD R + + L+ +P+ +R ++L N+ +
Sbjct: 157 SRRHLEFRELIKT--------MRNVDLCRTRKEILKDLESAIPDLAMRQFILTNLQPS-- 206
Query: 231 VVEYRSPVINYDDGLIHFKNPVRHMLDCGVKDVADWPTEEVKDKKFLGPVNFIRATKSAF 290
+ ++G + +K + + +D V ++ + + F GP F+ S +
Sbjct: 207 ---------SENEGQMEWKINI-NTIDSHVDEILGY---TLPVGSFRGPTLFLHGANSGY 253
Query: 291 INPDSLKAINSFFPFNNIHEI-NATHFVLNERPQEYLRAVIDFFK 334
+ D I FP I ++ H+V E+PQ ++ +V F K
Sbjct: 254 VPDDHKPDIKCLFPQVQFDAIPDSGHWVHAEKPQLFINSVYKFLK 298
>7299497 [R] KOG2382 Predicted alpha/beta hydrolase
Length = 306
Score = 65.5 bits (158), Expect = 1e-10
Identities = 57/269 (21%), Positives = 112/269 (41%), Gaps = 19/269 (7%)
Query: 67 PIIFFHGLLGSKRNYKHDCKKLATALQTPVYTVDVRNHGSSEHALPFNYGTLVNDLVHFI 126
PI+ H L S +++ L+ V TVD RNHG S + + L D+ +
Sbjct: 47 PIVVMHDLNLSLESWRQVAVNLSQVGLRQVITVDARNHGLSPYITGHSPMHLAADVEALM 106
Query: 127 HQHKLGKVNIIGYSLGAKVGMLACLKHPELFSAACIIDNAPEPQPHIKAFLSTLIKSCVK 186
+L K+ +G+ +G + M L P+L ++D P P P L + +
Sbjct: 107 SHQRLNKIVALGHGMGGRAMMTLALTQPQLVERVILVDITPAPVPS----NFYLTRQVFE 162
Query: 187 LLDQGKVRVDDKLWRHKASESLKRYLPNAGVRNYVLNNIVHNPRVVEYRSPVIN---YDD 243
++ Q + L SE LP + ++VH+ E R + N D
Sbjct: 163 MMLQVAPSIPSNL---SLSEGRTFILP-------LFQDVVHD--ASELRRIIYNLRKMQD 210
Query: 244 GLIHFKNPVRHMLDCGVKDVADWPTEEVKDKKFLGPVNFIRATKSAFINPDSLKAINSFF 303
+ + +L + + ++ + ++G V I ++S F+ S+ + +F
Sbjct: 211 NTFGWAVNPQAVLSSWGEMMINYEATLGGLRPYMGEVLLIAGSQSEFVTTTSIAVMQRYF 270
Query: 304 PFNNIHEINATHFVLNERPQEYLRAVIDF 332
P + ++A H V ++P++++ V++F
Sbjct: 271 PNTVVQILDAGHCVYEDQPEQFVELVVEF 299
>CE17746 [R] KOG2382 Predicted alpha/beta hydrolase
Length = 297
Score = 51.6 bits (122), Expect = 2e-06
Identities = 65/276 (23%), Positives = 109/276 (38%), Gaps = 34/276 (12%)
Query: 67 PIIFFHGLLGSKRNYKHDCKKLATALQTPVYTVDVRNHGSSEHALPFNYGTLVNDLVHFI 126
P+I GL G+K N+ K L+ L V+ V+ RNHGS A Y + +DLV FI
Sbjct: 37 PLILVPGLFGTKENWIQVGKDLSQRLGCMVFAVENRNHGSFSKAASMTYEEMADDLVGFI 96
Query: 127 HQHKL----GKVNIIGYSLGAK-VGMLACL-KHPELFSAACIIDNAPEPQPHIKAFLSTL 180
+ KVN+ G+S+G K V LA ++ + + D +P P +A
Sbjct: 97 DWVRKITGEDKVNLHGHSMGGKAVTQLATTPEYSSRIKSLIVEDMSPLGYPLKRAEYLEC 156
Query: 181 IKSCVKLLDQGKVRVDDKLWRHKASESLKRYLPNAGVRNYVLNNIVHNPRVVEYRSPVIN 240
IK + D R + L + + +V N+ + + +N
Sbjct: 157 IKQ--------MIATDMNKSRSEVMAELGEKVSKVLLYQFVRGNLGEDVNGKAHWICNLN 208
Query: 241 -YDDGLIHFKNPVRHMLDCGVKDVADWPTEEVKDKKFLGPVNFIRATKSAFINPDSLKAI 299
D+ I+ + H + GV D GP F RA S F+ +
Sbjct: 209 VIDETYIYL---LSHDIRFGVFD---------------GPTLFQRAPGSGFLPAAHKNRV 250
Query: 300 NSFFPFNNIHEIN-ATHFVLNERPQEYLRAVIDFFK 334
FP E + H++ + P+ ++ ++ +F +
Sbjct: 251 EKMFPMVQFAETAWSNHWIHADDPKFFVDSICEFLE 286
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.321 0.139 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,608,881
Number of Sequences: 60738
Number of extensions: 1029958
Number of successful extensions: 2205
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2188
Number of HSP's gapped (non-prelim): 10
length of query: 368
length of database: 30,389,216
effective HSP length: 108
effective length of query: 260
effective length of database: 23,829,512
effective search space: 6195673120
effective search space used: 6195673120
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)