ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactV6362    suspect: LH   U     KOG4699  Intracellular trafficking, secretion, and vesicular transport  Preprotein translocase subunit Sec66

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactV6362 2181446 2180841 -202 
         (202 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YBR171w [U] KOG4699 Preprotein translocase subunit Sec66              244   6e-65
SPBC409.21 [U] KOG4699 Preprotein translocase subunit Sec66            77   2e-14

>YBR171w [U] KOG4699 Preprotein translocase subunit Sec66
          Length = 206

 Score =  244 bits (623), Expect = 6e-65
 Identities = 118/201 (58%), Positives = 161/201 (79%), Gaps = 3/201 (1%)

Query: 4   NGTHF--NETFFEGQEK-PEIKVVSVYTPLVYVSILVISLMIFASKYRRSQVKKLAELPS 60
           N T F  N TFFE +E   E K +SVYTPL+YV ILV+SL++FAS YR+ Q KK++E PS
Sbjct: 5   NETKFSNNGTFFETEEPIVETKSISVYTPLIYVFILVVSLVMFASSYRKKQAKKISEQPS 64

Query: 61  IFDEHEARDLYFAIAELAETEQLHPKVVKAALLNRGAEAVRRTIKLREVAVQIEILYKNG 120
           IFDE++A DLYF I E++E E++H KV+KAALLNRGAE+VRR++KL+E+A QI +LYKNG
Sbjct: 65  IFDENDAHDLYFQIKEMSENEKIHEKVLKAALLNRGAESVRRSLKLKELAPQINLLYKNG 124

Query: 121 SVDDKYWQRYQNEMKLVDLELKSCIEEAERLQPDWPQAFVNLCREICFNQALQRRYDSIL 180
           S+ + YW+R++ E+KL++LE K  ++EAERLQP W Q FV +C+EICFNQAL RRY SIL
Sbjct: 125 SIGEDYWKRFETEVKLIELEFKDTLQEAERLQPGWVQLFVMVCKEICFNQALSRRYQSIL 184

Query: 181 DRKNIFQKLYQLKLDDNGKLI 201
            RK +  K ++LK++++G+L+
Sbjct: 185 KRKEVCIKEWELKINNDGRLV 205


>SPBC409.21 [U] KOG4699 Preprotein translocase subunit Sec66
          Length = 192

 Score = 76.6 bits (187), Expect = 2e-14
 Identities = 48/160 (30%), Positives = 84/160 (52%), Gaps = 7/160 (4%)

Query: 23  VVSVYTPLVYVSILVISLM---IFASKYRRSQVKKLAELPSIFDEHEARDLYFAIAELAE 79
           +VS+Y PL+Y++IL+ S+     F  K +  + K ++E    F E+ +R+++F++  L +
Sbjct: 1   MVSIYVPLIYITILMGSMYGVSRFVRKSKNQESKPVSE--EWFGENYSRNIFFSL--LQQ 56

Query: 80  TEQLHPKVVKAALLNRGAEAVRRTIKLREVAVQIEILYKNGSVDDKYWQRYQNEMKLVDL 139
                  ++KAAL+ R  E +RR +KL+   + +  L   G V D+  +++    K  +L
Sbjct: 57  NPPAEDTLLKAALVLRATEGLRRLMKLKVSRMALNNLLNRGGVGDELIRKFGRLEKETEL 116

Query: 140 ELKSCIEEAERLQPDWPQAFVNLCREICFNQALQRRYDSI 179
           EL    + A  LQP W Q     C EI  N+ +    D+I
Sbjct: 117 ELMDIAKTANSLQPGWNQFIFQTCNEIIENEKIHSIIDNI 156


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.321    0.137    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,491,840
Number of Sequences: 60738
Number of extensions: 436779
Number of successful extensions: 1203
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1200
Number of HSP's gapped (non-prelim): 2
length of query: 202
length of database: 30,389,216
effective HSP length: 101
effective length of query: 101
effective length of database: 24,254,678
effective search space: 2449722478
effective search space used: 2449722478
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)