ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV6370.1 suspect: Pn G KOG2431 Carbohydrate transport and metabolism 1, 2-alpha-mannosidase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV6370.1 2184451 2184020 -144
(144 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
Hs7706437 [G] KOG2431 1 2-alpha-mannosidase 32 0.28
Hs6005808 [G] KOG2431 1 2-alpha-mannosidase 32 0.28
CE09122 [G] KOG0626 Beta-glucosidase lactase phlorizinhydrolase ... 30 1.1
Hs6005766 [O] KOG3736 Polypeptide N-acetylgalactosaminyltransferase 29 1.8
CE04248 [G] KOG0626 Beta-glucosidase lactase phlorizinhydrolase ... 29 1.8
CE21095 [R] KOG0725 Reductases with broad range of substrate spe... 29 2.4
At1g07710 [R] KOG0504 FOG: Ankyrin repeat 29 2.4
HsM20127513 [JD] KOG2874 rRNA processing protein 28 3.1
Hs21359980 [JD] KOG2874 rRNA processing protein 28 3.1
At5g05400 [T] KOG4658 Apoptotic ATPase 28 3.1
At1g05640 [R] KOG0504 FOG: Ankyrin repeat 28 3.1
SPAC20H4.10 [O] KOG2042 Ubiquitin fusion degradation protein-2 28 4.1
YDR097c [L] KOG0217 Mismatch repair ATPase MSH6 (MutS family) 28 5.3
CE12990 [R] KOG0725 Reductases with broad range of substrate spe... 28 5.3
At5g60070 [R] KOG0504 FOG: Ankyrin repeat 28 5.3
At2g31820 [R] KOG0504 FOG: Ankyrin repeat 28 5.3
At1g74190 [R] KOG0619 FOG: Leucine rich repeat 27 9.1
>Hs7706437 [G] KOG2431 1 2-alpha-mannosidase
Length = 699
Score = 32.0 bits (71), Expect = 0.28
Identities = 22/88 (25%), Positives = 34/88 (38%), Gaps = 17/88 (19%)
Query: 6 PGWNSDSNIGDVTLGLFN-------------NGGLDLVNEHCHHLSGRID----LLFTTK 48
P W SDS + +VT ++ V +H H LSG+ D + T
Sbjct: 387 PRWTSDSTVAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSGKKDGLVPMFINTH 446
Query: 49 SDLGTNLTIIQLNQLCGKVLAFFLEVWV 76
S L T+L + L + L+ W+
Sbjct: 447 SGLFTHLGVFTLGARADSYYEYLLKQWI 474
>Hs6005808 [G] KOG2431 1 2-alpha-mannosidase
Length = 663
Score = 32.0 bits (71), Expect = 0.28
Identities = 22/88 (25%), Positives = 34/88 (38%), Gaps = 17/88 (19%)
Query: 6 PGWNSDSNIGDVTLGLFN-------------NGGLDLVNEHCHHLSGRID----LLFTTK 48
P W SDS + +VT ++ V +H H LSG+ D + T
Sbjct: 351 PRWTSDSTVAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSGKKDGLVPMFINTH 410
Query: 49 SDLGTNLTIIQLNQLCGKVLAFFLEVWV 76
S L T+L + L + L+ W+
Sbjct: 411 SGLFTHLGVFTLGARADSYYEYLLKQWI 438
>CE09122 [G] KOG0626 Beta-glucosidase lactase phlorizinhydrolase and related
proteins
Length = 475
Score = 30.0 bits (66), Expect = 1.1
Identities = 20/63 (31%), Positives = 31/63 (48%), Gaps = 2/63 (3%)
Query: 16 DVTLGLFNNGGLDLVNEHCHHLSGRIDLLFTTKSDL-GTNLTIIQLN-QLCGKVLAFFLE 73
D+ L +++NG L E+C H DL F DL T +T ++N Q G ++ E
Sbjct: 125 DMPLAIYDNGTAWLNRENCEHFEKFADLCFQKFGDLVKTWITYNEINCQAWGSIVKVEGE 184
Query: 74 VWV 76
W+
Sbjct: 185 FWL 187
>Hs6005766 [O] KOG3736 Polypeptide N-acetylgalactosaminyltransferase
Length = 622
Score = 29.3 bits (64), Expect = 1.8
Identities = 17/46 (36%), Positives = 22/46 (46%), Gaps = 4/46 (8%)
Query: 19 LGLFNNGGLDLVNEHCHHLSGRIDLLFTTKSDLGTNLTIIQLNQLC 64
+G N GG L+ CH L G +TT+ DL N+ QLC
Sbjct: 512 VGENNRGGKPLIMYSCHGLGGNQYFEYTTQRDLRHNIA----KQLC 553
>CE04248 [G] KOG0626 Beta-glucosidase lactase phlorizinhydrolase and related
proteins
Length = 479
Score = 29.3 bits (64), Expect = 1.8
Identities = 20/63 (31%), Positives = 31/63 (48%), Gaps = 2/63 (3%)
Query: 16 DVTLGLFNNGGLDLVNEHCHHLSGRIDLLFTTKSDL-GTNLTIIQLN-QLCGKVLAFFLE 73
D+ L +++NG L E+C H DL F DL T +T ++N Q V+ E
Sbjct: 125 DMPLSIYDNGTSWLNKENCEHFEKFADLCFQKFGDLVKTWITFNEINMQAWSSVVKIEGE 184
Query: 74 VWV 76
+W+
Sbjct: 185 LWL 187
>CE21095 [R] KOG0725 Reductases with broad range of substrate specificities
Length = 280
Score = 28.9 bits (63), Expect = 2.4
Identities = 13/43 (30%), Positives = 23/43 (53%), Gaps = 1/43 (2%)
Query: 100 LTRFTNKSVLLTETDQSWGRTVG-NFVHNNVNTSVSGNSQNSI 141
+ RF+NK+V++T + GRT F N +++G S +
Sbjct: 1 MPRFSNKTVIITGSSNGIGRTTAILFAQEGANVTITGRSSERL 43
>At1g07710 [R] KOG0504 FOG: Ankyrin repeat
Length = 543
Score = 28.9 bits (63), Expect = 2.4
Identities = 15/60 (25%), Positives = 30/60 (50%), Gaps = 10/60 (16%)
Query: 68 LAFFLEVWVVVVQTN----------DSLQVVNSVLNVGCLLVLTRFTNKSVLLTETDQSW 117
+A F+ + VVVVQT+ + V+N ++ + C+L+ F S ++ ++ W
Sbjct: 414 IALFISLAVVVVQTSVVVIESKAKKQMMAVINKLMWLACVLISVAFLALSFVVVGEEEKW 473
>HsM20127513 [JD] KOG2874 rRNA processing protein
Length = 381
Score = 28.5 bits (62), Expect = 3.1
Identities = 14/48 (29%), Positives = 25/48 (51%), Gaps = 3/48 (6%)
Query: 83 DSLQVVNSVLNVGCLLVLTRFTNKSVLLTETDQSWGRTVGNFVHNNVN 130
D+++ ++ + N+ L++ S L + QSW R + F H NVN
Sbjct: 208 DTMKNIHPIYNIKSLMIKRELAKDSELRS---QSWERFLPQFKHKNVN 252
>Hs21359980 [JD] KOG2874 rRNA processing protein
Length = 381
Score = 28.5 bits (62), Expect = 3.1
Identities = 14/48 (29%), Positives = 25/48 (51%), Gaps = 3/48 (6%)
Query: 83 DSLQVVNSVLNVGCLLVLTRFTNKSVLLTETDQSWGRTVGNFVHNNVN 130
D+++ ++ + N+ L++ S L + QSW R + F H NVN
Sbjct: 208 DTMKNIHPIYNIKSLMIKRELAKDSELRS---QSWERFLPQFKHKNVN 252
>At5g05400 [T] KOG4658 Apoptotic ATPase
Length = 874
Score = 28.5 bits (62), Expect = 3.1
Identities = 23/99 (23%), Positives = 41/99 (41%), Gaps = 8/99 (8%)
Query: 8 WNSDSNIGDVTLGLFNNGG------LDLVNEHCHHLSGRIDLLFTTKSDLGTNLTIIQLN 61
WNS +G LG++ GG L +N +S D+ + N T+ ++
Sbjct: 167 WNSMMEVGVGLLGIYGMGGVGKTTLLSQINNKFRTVSNDFDIAIWVV--VSKNPTVKRIQ 224
Query: 62 QLCGKVLAFFLEVWVVVVQTNDSLQVVNSVLNVGCLLVL 100
+ GK L + E W + + + S+ N +L+L
Sbjct: 225 EDIGKRLDLYNEGWEQKTENEIASTIKRSLENKKYMLLL 263
>At1g05640 [R] KOG0504 FOG: Ankyrin repeat
Length = 627
Score = 28.5 bits (62), Expect = 3.1
Identities = 16/60 (26%), Positives = 30/60 (49%), Gaps = 10/60 (16%)
Query: 68 LAFFLEVWVVVVQTN----------DSLQVVNSVLNVGCLLVLTRFTNKSVLLTETDQSW 117
LA F+ + VVVVQT+ + + V+N ++ + CL + F + S ++ + W
Sbjct: 498 LALFISLAVVVVQTSVVVIEQKAKKNLVFVINKLMWLACLFISVAFVSLSFIVVGKEDIW 557
>SPAC20H4.10 [O] KOG2042 Ubiquitin fusion degradation protein-2
Length = 1010
Score = 28.1 bits (61), Expect = 4.1
Identities = 24/122 (19%), Positives = 52/122 (41%), Gaps = 3/122 (2%)
Query: 5 VPGWNSDSNIGDVTLGLFNNGGLDLVNEHCHHLSGRIDLLFTTKSDLGTNLTIIQLNQLC 64
+P WN +N G++ F G + ++ ++ R T +S + +I L
Sbjct: 272 LPSWNPTNNAGEIEYKTF-LGRISSLSVFTQDVASRYFSNSTERSAQNISSSISSLKLTM 330
Query: 65 GKVLAFFLEVWVVVVQTNDSLQVVNSVLNVGCLLVLTRFTNKSVLLTETDQSWGRTVGNF 124
+++ +++T+ SL+ SVL+ ++V +S+ + D + + NF
Sbjct: 331 STYQDVLFQIFNTLIRTSTSLR--ESVLDFFAMVVNANHKRQSIQVNHFDITSDACMLNF 388
Query: 125 VH 126
H
Sbjct: 389 SH 390
>YDR097c [L] KOG0217 Mismatch repair ATPase MSH6 (MutS family)
Length = 1242
Score = 27.7 bits (60), Expect = 5.3
Identities = 13/42 (30%), Positives = 22/42 (51%), Gaps = 9/42 (21%)
Query: 61 NQLCGKVLAFFLEVWVVVVQTNDSLQVVNSVLNVGCLLVLTR 102
N+LC K A + +W+ +Q ++ N+ CLL +TR
Sbjct: 881 NRLCQKFDAHYNTIWMPTIQ---------AISNIDCLLAITR 913
>CE12990 [R] KOG0725 Reductases with broad range of substrate specificities
Length = 279
Score = 27.7 bits (60), Expect = 5.3
Identities = 14/46 (30%), Positives = 23/46 (49%), Gaps = 1/46 (2%)
Query: 100 LTRFTNKSVLLTETDQSWGR-TVGNFVHNNVNTSVSGNSQNSIVIT 144
+TRF KSV++T + GR T F N +++G + + T
Sbjct: 1 MTRFAGKSVIITGSSSGIGRATAVLFAKNGAQVTITGRNAEKLEAT 46
>At5g60070 [R] KOG0504 FOG: Ankyrin repeat
Length = 548
Score = 27.7 bits (60), Expect = 5.3
Identities = 14/60 (23%), Positives = 31/60 (51%), Gaps = 10/60 (16%)
Query: 68 LAFFLEVWVVVVQTN----------DSLQVVNSVLNVGCLLVLTRFTNKSVLLTETDQSW 117
+A F+ + VVVVQT+ + + V+N ++ + C+L+ F + ++ ++ W
Sbjct: 425 IALFISLAVVVVQTSVVAIEHKAKKNMMAVINKLMWLACVLISVAFLALAFVVVGEEERW 484
>At2g31820 [R] KOG0504 FOG: Ankyrin repeat
Length = 662
Score = 27.7 bits (60), Expect = 5.3
Identities = 16/60 (26%), Positives = 29/60 (47%), Gaps = 10/60 (16%)
Query: 68 LAFFLEVWVVVVQTN----------DSLQVVNSVLNVGCLLVLTRFTNKSVLLTETDQSW 117
LA F+ + VVVVQT+ + V+N ++ CL + F + S ++ ++ W
Sbjct: 533 LALFISLAVVVVQTSVVVIEQKAKKKLVFVINKLMWCACLFISIAFVSLSYIVVGKEEMW 592
>At1g74190 [R] KOG0619 FOG: Leucine rich repeat
Length = 965
Score = 26.9 bits (58), Expect = 9.1
Identities = 15/53 (28%), Positives = 29/53 (54%), Gaps = 3/53 (5%)
Query: 15 GDVTLGLFNNGGLDLVNEHCHHLSGRIDLLFTTKSDLGTNLTIIQLNQLCGKV 67
GD+ + LFN L L++ + LSG I +++ + + ++Q N+L G +
Sbjct: 573 GDIPMSLFNKSSLQLLDLSANSLSGVIPPQHDSRNGV---VLLLQDNKLSGTI 622
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.136 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,976,837
Number of Sequences: 60738
Number of extensions: 285539
Number of successful extensions: 625
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 619
Number of HSP's gapped (non-prelim): 17
length of query: 144
length of database: 30,389,216
effective HSP length: 96
effective length of query: 48
effective length of database: 24,558,368
effective search space: 1178801664
effective search space used: 1178801664
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)