ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV6420 good R KOG0254 General function prediction only Predicted transporter (major facilitator superfamily)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV6420 2201902 2202573 224
(224 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F8.03c [R] KOG0254 Predicted transporter (major facilitator... 141 8e-34
SPBC4F6.09 [R] KOG0254 Predicted transporter (major facilitator ... 72 8e-13
YKR106w [R] KOG0254 Predicted transporter (major facilitator sup... 70 2e-12
YEL065w [R] KOG0254 Predicted transporter (major facilitator sup... 70 2e-12
YCL073c [R] KOG0254 Predicted transporter (major facilitator sup... 69 7e-12
YHL040c [R] KOG0254 Predicted transporter (major facilitator sup... 64 2e-10
YHL047c [R] KOG0254 Predicted transporter (major facilitator sup... 63 3e-10
YOL158c [R] KOG0254 Predicted transporter (major facilitator sup... 62 5e-10
SPCC61.01c [R] KOG0254 Predicted transporter (major facilitator ... 50 3e-06
>SPAC1F8.03c [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 630
Score = 141 bits (355), Expect = 8e-34
Identities = 81/213 (38%), Positives = 121/213 (56%), Gaps = 9/213 (4%)
Query: 1 MGLCFFGVFWGALFRVFRRYKIFQVTGIAIKLIGMGLYVLCSTKEDGPHLGLVVAALIIT 60
+ LC FGVF GA+ RVF RYK Q+ G+ IK++G G+ + + G + +LI+
Sbjct: 416 IALCVFGVFAGAMHRVFHRYKYLQIIGLVIKIVGYGILIRPNFAATGKVD--LAWSLILI 473
Query: 61 CFGDAANVMGTQVAAQAAVPHQDMAATISVLSLYSSIGAAVGSAITSAVWTDHLPKALYK 120
G + +V+G+QV+ QA+VPHQD+A S+L LY++IG A+G+AI S ++++ +PK L +
Sbjct: 474 GMGGSFSVVGSQVSCQASVPHQDLAIASSLLPLYTNIGGAIGAAIASPIFSNKVPKYLRE 533
Query: 121 YIPXX---XXXXXXXXXXXXXXXQPWGTPTRLAAIHAYQDVLYTLFCMGVGVSSIMFIAC 177
Y+P P GT R AI AY ++ L V +S I A
Sbjct: 534 YLPSSINDTQVYNFYSDSSLIREYPVGTEIRDGAIKAYSRSMFFLLVPAVSLSFIPLAAA 593
Query: 178 LFQTNFYLGDQLNCVEGEQVEDYNHNPDGSKKT 210
+Q+NFYLG+Q N VEG+Q +H G K+T
Sbjct: 594 FWQSNFYLGNQQNAVEGDQ----DHKKKGDKET 622
>SPBC4F6.09 [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 612
Score = 71.6 bits (174), Expect = 8e-13
Identities = 51/181 (28%), Positives = 77/181 (42%), Gaps = 4/181 (2%)
Query: 4 CFFGVFWGALFRVFRRYKIFQVTGIAIKLIGMGLYVLCSTKEDGPHLGLVVAALIITCFG 63
C G F G L R+ +RYK + V I + ++G GL + + + + G + II G
Sbjct: 416 CATGFFLGILIRLTKRYKWYFVASIPVYILGQGLMI--RYRGEQYNWGYQIMPQIIVGIG 473
Query: 64 DAANVMGTQVAAQAAVPHQDMAATISVLSLYSSIGAAVGSAITSAVWTDHLPKALYKYIP 123
VA Q V ++ A +++S + IG AVGSAI+ A+W +PK L K +P
Sbjct: 474 GGVIANLLTVAVQTVVSTENFAIVTALVSTVTPIGGAVGSAISGAIWNSVMPKRLEKNLP 533
Query: 124 XXXXXXXXXXXXXXXXXQPW--GTPTRLAAIHAYQDVLYTLFCMGVGVSSIMFIACLFQT 181
+ GT R A I +Y +V L + G + M F
Sbjct: 534 SDLKDQAYTIFESLTVQLSYTRGTDARNAIILSYSEVQKILTSVATGFAGAMIFPVWFVA 593
Query: 182 N 182
N
Sbjct: 594 N 594
>YKR106w [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 615
Score = 70.5 bits (171), Expect = 2e-12
Identities = 47/181 (25%), Positives = 81/181 (43%), Gaps = 3/181 (1%)
Query: 9 FWGALFRVFRRYKIFQVTGIAIKLIGMGLYVLCSTKEDGPHLGLVVAALIITCFGDAANV 68
F+ L R+ K+ + G A ++ MGL+ G H G++ A++I+ G +
Sbjct: 398 FYSLLVAKTRKLKLSVIGGCAAWMVCMGLFYKYRGGS-GSHEGVIAASVIMGLSGLLCS- 455
Query: 69 MGTQVAAQAAVPHQDMAATISVLSLYSSIGAAVGSAITSAVWTDHLPKALYKYIPXXXXX 128
V QA H MA + +S +GAA+G++++ A+WT +P LYK +
Sbjct: 456 NSVIVILQAMTTHSRMAVITGIQYTFSKLGAAIGASVSGAIWTQTMPNQLYKNLGNDTLA 515
Query: 129 XXXXXX-XXXXXXQPWGTPTRLAAIHAYQDVLYTLFCMGVGVSSIMFIACLFQTNFYLGD 187
PWG+P R A + +Y+ V + +G+ + F +F N L D
Sbjct: 516 EIAYASPYTFISDYPWGSPERDAVVESYRYVQRIIMTVGLACTVPFFTFTMFMRNPELID 575
Query: 188 Q 188
+
Sbjct: 576 K 576
>YEL065w [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 628
Score = 70.1 bits (170), Expect = 2e-12
Identities = 58/198 (29%), Positives = 88/198 (44%), Gaps = 6/198 (3%)
Query: 7 GVFWGALFRVFRRYKIFQVTGIAIKLIGMGLYVLCSTKEDGPHLGLVVAALIITCFGDAA 66
G G + RR K F + GI+ ++ GL V + G H G ++ +L + FG +
Sbjct: 406 GTILGFILIKVRRTKPFIIFGISCWIVSFGLLVHYRG-DSGAHSG-IIGSLCLLGFGAGS 463
Query: 67 NVMGTQVAAQA-AVPHQDMAATISVLSLYSSIGAAVGSAITSAVWTDHLPKALYKYIPX- 124
TQ + QA A H MA S+ +IG+A GS+++ AVWT+ LPK + K I
Sbjct: 464 FTYVTQASIQASAKTHARMAVVTSLYLATYNIGSAFGSSVSGAVWTNILPKEISKRISDP 523
Query: 125 XXXXXXXXXXXXXXXXQPWGTPTRLAAIHAYQDVLYTLFCMGVGVSSIMFIACLFQ-TNF 183
WGTP R+A + +Y+ V L +G+ V + C F N
Sbjct: 524 TLAAQAYGSPFTFITTYTWGTPERIALVMSYRYVQKILCIIGL-VFCFPLLGCAFMLRNH 582
Query: 184 YLGDQLNCVEGEQVEDYN 201
L D + + +E N
Sbjct: 583 KLTDSIALEGNDHLESKN 600
>YCL073c [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 615
Score = 68.6 bits (166), Expect = 7e-12
Identities = 46/181 (25%), Positives = 81/181 (44%), Gaps = 3/181 (1%)
Query: 9 FWGALFRVFRRYKIFQVTGIAIKLIGMGLYVLCSTKEDGPHLGLVVAALIITCFGDAANV 68
F+ L R+ K+ + G A ++ MGL+ G H G++ A++I+ G +
Sbjct: 398 FYSLLVAKTRKLKLSVIGGCAAWMVCMGLFYKYRGGS-GSHEGVIAASVIMGLSGLLCS- 455
Query: 69 MGTQVAAQAAVPHQDMAATISVLSLYSSIGAAVGSAITSAVWTDHLPKALYKYIPXXXXX 128
V QA H MA + +S +GAA+G++++ A+WT +P LYK +
Sbjct: 456 NSVIVILQAMTTHSRMAVITGIQYTFSKLGAAIGASVSGAIWTQTMPNQLYKNLGNDTLA 515
Query: 129 XXXXXX-XXXXXXQPWGTPTRLAAIHAYQDVLYTLFCMGVGVSSIMFIACLFQTNFYLGD 187
PWG+P R A + +Y+ V + +G+ + F +F + L D
Sbjct: 516 EIAYASPYTFISDYPWGSPERDAVVESYRYVQRIIMTVGLACTVPFFAFTMFMRDPELID 575
Query: 188 Q 188
+
Sbjct: 576 K 576
>YHL040c [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 627
Score = 63.5 bits (153), Expect = 2e-10
Identities = 56/200 (28%), Positives = 87/200 (43%), Gaps = 6/200 (3%)
Query: 9 FWGALFRVFRRYKIFQVTGIAIKLIGMGLYVLCSTKEDGPHLGLVVAALIITCFGDAANV 68
F+ L R K F + G A+ ++ MGL H G ++ AL + G
Sbjct: 413 FFALLVTRCTRLKPFIMFGCALWMVAMGLLYHFRGGSQS-HSG-IIGALCVWGVGTTLFT 470
Query: 69 MGTQVAAQAAVPHQDMAATISVLSLYSSIGAAVGSAITSAVWTDHLPKALYKYI-PXXXX 127
V+ Q+AV H++MA ++ IG+AVGSA++ A+WT L K + K +
Sbjct: 471 YPVTVSVQSAVSHENMATVTALNYTLYRIGSAVGSAVSGAIWTQTLYKQILKRMGDVALA 530
Query: 128 XXXXXXXXXXXXXQPWGTPTRLAAIHAYQDV--LYTLFCMGVGVSSIMFIACLFQTNFYL 185
WGTP R A ++AY+ V L T+ + V I F CL
Sbjct: 531 TTAYESPYTFIETYTWGTPQRNALMNAYKYVQRLETIVALVFCVPLIAFSLCLRDPKLTD 590
Query: 186 GDQLNCVE-GEQVEDYNHNP 204
+ +E GE V+ +++P
Sbjct: 591 TVAVEYIEDGEYVDTKDNDP 610
>YHL047c [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 637
Score = 63.2 bits (152), Expect = 3e-10
Identities = 45/168 (26%), Positives = 73/168 (42%), Gaps = 3/168 (1%)
Query: 9 FWGALFRVFRRYKIFQVTGIAIKLIGMGLYVLCSTKEDGPHLGLVVAALIITCFGDAANV 68
F G + R K + + G ++ I MGL+ + +D G ++A ++I
Sbjct: 408 FLGLIVTRSSRLKSYIIFGGSLYFITMGLFYRYRSGQDAD--GGIIAGMVIWGLSSCLFD 465
Query: 69 MGTQVAAQAAVPHQDMAATISVLSLYSSIGAAVGSAITSAVWTDHL-PKALYKYIPXXXX 127
T V+ Q+ H++MA ++ IG AV +AI+ A+WT L PK L+
Sbjct: 466 YPTIVSIQSVTSHENMATVTALNYTVFRIGGAVAAAISGAIWTQSLYPKLLHYMGDADLA 525
Query: 128 XXXXXXXXXXXXXQPWGTPTRLAAIHAYQDVLYTLFCMGVGVSSIMFI 175
PWGTP R A + AY+ V + + S+ MF+
Sbjct: 526 TAAYGSPLTFILSNPWGTPVRSAMVEAYRHVQKYEVIVALVFSAPMFL 573
>YOL158c [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 606
Score = 62.4 bits (150), Expect = 5e-10
Identities = 45/174 (25%), Positives = 71/174 (39%), Gaps = 4/174 (2%)
Query: 11 GALFRVFRRYKIFQVTGIAIKLIGMGLYVLCSTKEDGPHLGLVVAALIITCFGDAANV-M 69
G + + K+F V G + +IGMGL DG GL+ A +++ G M
Sbjct: 404 GIMLHFYPHPKVFVVAGSLLGVIGMGLLYKYRVVYDGIS-GLIGAEIVVGIAGGMIRFPM 462
Query: 70 GTQVAAQAAVPHQDMAATISVLSLYSSIGAAVGSAITSAVWTDHLPKALYKYIPXXXXXX 129
T V A+ H +MA +L IG AVG++I A+WT L K L + +
Sbjct: 463 WTLV--HASTTHNEMATVTGLLMSVYQIGDAVGASIAGAIWTQRLAKELIQRLGSSLGMA 520
Query: 130 XXXXXXXXXXXQPWGTPTRLAAIHAYQDVLYTLFCMGVGVSSIMFIACLFQTNF 183
P G+ R+ I +Y + L + + ++ + C F F
Sbjct: 521 IYKSPLNYLKKYPIGSEVRVQMIESYSKIQRLLIIVSISFAAFNAVLCFFLRGF 574
>SPCC61.01c [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 597
Score = 50.1 bits (118), Expect = 3e-06
Identities = 28/108 (25%), Positives = 52/108 (47%), Gaps = 1/108 (0%)
Query: 11 GALFRVFRRYKIFQVTGIAIKLIGMGLYVLCSTKEDGPHLGLVVAALIITCFGDAANVMG 70
G L + F R+K+ + + + ++G+ L ++ D + +V LI+ G +
Sbjct: 407 GFLMKRFGRFKMLLMISVPMYVLGI-LGIILFGINDNHYTRPLVLVLILAGMGGGLLTLS 465
Query: 71 TQVAAQAAVPHQDMAATISVLSLYSSIGAAVGSAITSAVWTDHLPKAL 118
Q+A Q+ H + +++ +SS+G A GSAI VW+ L L
Sbjct: 466 AQIAVQSVSSHAKLGMNLTLYLTFSSVGGAFGSAIAGGVWSKRLSSRL 513
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.327 0.141 0.447
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,016,736
Number of Sequences: 60738
Number of extensions: 433066
Number of successful extensions: 1175
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 1164
Number of HSP's gapped (non-prelim): 10
length of query: 224
length of database: 30,389,216
effective HSP length: 102
effective length of query: 122
effective length of database: 24,193,940
effective search space: 2951660680
effective search space used: 2951660680
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)