ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI0192 good K KOG2652 Transcription RNA polymerase II transcription initiation factor TFIIA, large chain
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI0192 65218 64532 -229
(229 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR194c [K] KOG2652 RNA polymerase II transcription initiation f... 140 2e-33
SPBC28F2.09 [K] KOG2652 RNA polymerase II transcription initiati... 62 7e-10
ECU03g1070 [K] KOG2652 RNA polymerase II transcription initiatio... 58 1e-08
CE12128 [K] KOG2652 RNA polymerase II transcription initiation f... 58 1e-08
7301567 [K] KOG2652 RNA polymerase II transcription initiation f... 58 1e-08
Hs7706735 [K] KOG2652 RNA polymerase II transcription initiation... 57 2e-08
Hs5802972 [K] KOG2652 RNA polymerase II transcription initiation... 55 1e-07
Hs5803153_2 [K] KOG2652 RNA polymerase II transcription initiati... 55 1e-07
At1g07480 [K] KOG2652 RNA polymerase II transcription initiation... 52 5e-07
At1g07470 [K] KOG2652 RNA polymerase II transcription initiation... 50 3e-06
>YOR194c [K] KOG2652 RNA polymerase II transcription initiation factor TFIIA
large chain
Length = 286
Score = 140 bits (352), Expect = 2e-33
Identities = 96/289 (33%), Positives = 138/289 (47%), Gaps = 64/289 (22%)
Query: 1 MSNIEASKVYETVLETVINDVRQEFEDSGVDEQTLQDLKRIWQMKLSESKAATFIWDPE- 59
MSN EAS+VYE ++E+V+N+VR++FE++G+DEQTLQDLK IWQ KL+E+K TF WD +
Sbjct: 1 MSNAEASRVYEIIVESVVNEVREDFENAGIDEQTLQDLKNIWQKKLTETKVTTFSWDNQF 60
Query: 60 --------------------------NTTSASTVAEPVVKLPNGEFNDN----------- 82
N +T E ++ LPN N+N
Sbjct: 61 NEGNINGVQNDLNFNLATPGVNSSEFNIKEENTGNEGLI-LPNINSNNNIPHSGETNINT 119
Query: 83 ----SINHSGAAASAG-----EADI-----------IGSNINNGSSDMVNN--DELQIED 120
+ N+SGA + AD+ I INN + V N DE + +D
Sbjct: 120 NTVEATNNSGATLNTNTSGNTNADVTSQPKIEVKPEIELTINNANITTVENIDDESEKKD 179
Query: 121 NEMKPKQEIEFSMNDPDGDXXXXXXXXXXXXXXXXXXETXXXXXXXXXXXXXXXXXXXXX 180
+E K ++++E + + + +T
Sbjct: 180 DEEK-EEDVEKTRKEK--EQIEQVKLQAKKEKRSALLDTDEVGSELDDSDDDYLISEGEE 236
Query: 181 EGTDVNMMLCLYDKVLRVKNKWKCNLKEGVVTIDHKDFAFQKAQGESEW 229
+G D N+MLCLYDKV R K +WKC+LK+GVVTI+ D+ FQKAQ E+EW
Sbjct: 237 DGPDENLMLCLYDKVTRTKARWKCSLKDGVVTINRNDYTFQKAQVEAEW 285
>SPBC28F2.09 [K] KOG2652 RNA polymerase II transcription initiation factor TFIIA
large chain
Length = 369
Score = 62.0 bits (149), Expect = 7e-10
Identities = 28/51 (54%), Positives = 37/51 (71%), Gaps = 2/51 (3%)
Query: 181 EGTDVN--MMLCLYDKVLRVKNKWKCNLKEGVVTIDHKDFAFQKAQGESEW 229
EG+D+ ++LCLYDKV KNKWKC ++GVV ++ KD+ F KA GE EW
Sbjct: 318 EGSDIGQAIVLCLYDKVNHHKNKWKCVFRDGVVGVNGKDYLFFKANGEFEW 368
Score = 54.7 bits (130), Expect = 1e-07
Identities = 29/73 (39%), Positives = 43/73 (58%), Gaps = 2/73 (2%)
Query: 1 MSNIEASKVYETVLETVINDVRQEFEDSGVDEQTLQDLKRIWQMKLSESKAATFIW--DP 58
MSN +VY V+ VI + R +FE++GVD+ TL++L+ +WQ KL + ATF W P
Sbjct: 1 MSNSIVGEVYHHVILDVIANSRSDFEENGVDDATLRELQNLWQSKLVATDVATFPWAQAP 60
Query: 59 ENTTSASTVAEPV 71
T + +PV
Sbjct: 61 VGTFPIGQLFDPV 73
>ECU03g1070 [K] KOG2652 RNA polymerase II transcription initiation factor TFIIA
large chain
Length = 157
Score = 58.2 bits (139), Expect = 1e-08
Identities = 25/49 (51%), Positives = 32/49 (65%)
Query: 181 EGTDVNMMLCLYDKVLRVKNKWKCNLKEGVVTIDHKDFAFQKAQGESEW 229
+G+ N M+CLY KV K KWKC K+G ++I + DF F AQGE EW
Sbjct: 109 KGSTGNCMVCLYVKVNMSKGKWKCTFKQGFISIGNIDFVFNSAQGELEW 157
>CE12128 [K] KOG2652 RNA polymerase II transcription initiation factor TFIIA
large chain
Length = 354
Score = 58.2 bits (139), Expect = 1e-08
Identities = 22/44 (50%), Positives = 33/44 (75%)
Query: 186 NMMLCLYDKVLRVKNKWKCNLKEGVVTIDHKDFAFQKAQGESEW 229
N+++C ++KV R + KWK LK+G++ ID KD+ FQK GE+EW
Sbjct: 311 NVVMCQFEKVNRARTKWKFQLKDGIMHIDKKDYCFQKCTGEAEW 354
>7301567 [K] KOG2652 RNA polymerase II transcription initiation factor TFIIA
large chain
Length = 363
Score = 57.8 bits (138), Expect = 1e-08
Identities = 21/44 (47%), Positives = 34/44 (76%)
Query: 186 NMMLCLYDKVLRVKNKWKCNLKEGVVTIDHKDFAFQKAQGESEW 229
N+++C YDK+ R +NKWK LK+G++ + KD+ FQK+ G++EW
Sbjct: 320 NVIVCQYDKITRSRNKWKFYLKDGIMNMRGKDYVFQKSNGDAEW 363
Score = 54.7 bits (130), Expect = 1e-07
Identities = 35/100 (35%), Positives = 48/100 (48%), Gaps = 5/100 (5%)
Query: 8 KVYETVLETVINDVRQEFEDSGVDEQTLQDLKRIWQMKLSESKAATFIWDPENTTSASTV 67
KVY V+E VI +VR F D GVDEQ LQ++K++W+ KL SKA D S
Sbjct: 10 KVYHAVIEDVITNVRDAFLDEGVDEQVLQEMKQVWRNKLLASKAVELSPD-----SGDGS 64
Query: 68 AEPVVKLPNGEFNDNSINHSGAAASAGEADIIGSNINNGS 107
P + N + + + AA + IG N + S
Sbjct: 65 HPPPIVANNPKAANAKAKKAAAATAVTSHQHIGGNSSMSS 104
>Hs7706735 [K] KOG2652 RNA polymerase II transcription initiation factor TFIIA
large chain
Length = 376
Score = 57.4 bits (137), Expect = 2e-08
Identities = 21/44 (47%), Positives = 35/44 (78%)
Query: 186 NMMLCLYDKVLRVKNKWKCNLKEGVVTIDHKDFAFQKAQGESEW 229
N+++C YDK+ R KNKWK +LK+G++ ++ +D+ F KA G++EW
Sbjct: 333 NVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW 376
Score = 55.5 bits (132), Expect = 6e-08
Identities = 25/44 (56%), Positives = 33/44 (74%)
Query: 8 KVYETVLETVINDVRQEFEDSGVDEQTLQDLKRIWQMKLSESKA 51
K+Y +V+E VINDVR F D GVDEQ L +LK +W+ KL +S+A
Sbjct: 12 KLYRSVIEDVINDVRDIFLDDGVDEQVLMELKTLWENKLMQSRA 55
>Hs5802972 [K] KOG2652 RNA polymerase II transcription initiation factor TFIIA
large chain
Length = 478
Score = 54.7 bits (130), Expect = 1e-07
Identities = 22/44 (50%), Positives = 32/44 (72%)
Query: 186 NMMLCLYDKVLRVKNKWKCNLKEGVVTIDHKDFAFQKAQGESEW 229
N+++C YDK+ R KNKWK LK+GV+ +D+ F KA G++EW
Sbjct: 435 NVIVCQYDKIHRSKNKWKFYLKDGVMCFGGRDYVFAKAIGDAEW 478
Score = 50.4 bits (119), Expect = 2e-06
Identities = 21/44 (47%), Positives = 33/44 (74%)
Query: 8 KVYETVLETVINDVRQEFEDSGVDEQTLQDLKRIWQMKLSESKA 51
K+Y +V+E VI VR F + G++EQ L+DLK++W+ K+ +SKA
Sbjct: 9 KLYRSVIEDVIEGVRNLFAEEGIEEQVLKDLKQLWETKVLQSKA 52
>Hs5803153_2 [K] KOG2652 RNA polymerase II transcription initiation factor TFIIA
large chain
Length = 474
Score = 54.7 bits (130), Expect = 1e-07
Identities = 22/44 (50%), Positives = 32/44 (72%)
Query: 186 NMMLCLYDKVLRVKNKWKCNLKEGVVTIDHKDFAFQKAQGESEW 229
N+++C YDK+ R KNKWK LK+GV+ +D+ F KA G++EW
Sbjct: 431 NVIVCQYDKIHRSKNKWKFYLKDGVMCFGGRDYVFAKAIGDAEW 474
Score = 50.4 bits (119), Expect = 2e-06
Identities = 21/44 (47%), Positives = 33/44 (74%)
Query: 8 KVYETVLETVINDVRQEFEDSGVDEQTLQDLKRIWQMKLSESKA 51
K+Y +V+E VI VR F + G++EQ L+DLK++W+ K+ +SKA
Sbjct: 5 KLYRSVIEDVIEGVRNLFAEEGIEEQVLKDLKQLWETKVLQSKA 48
>At1g07480 [K] KOG2652 RNA polymerase II transcription initiation factor TFIIA
large chain
Length = 375
Score = 52.4 bits (124), Expect = 5e-07
Identities = 23/51 (45%), Positives = 36/51 (70%), Gaps = 3/51 (5%)
Query: 182 GTDVN---MMLCLYDKVLRVKNKWKCNLKEGVVTIDHKDFAFQKAQGESEW 229
G D+N ++L +DKV R K++WKC+LK+G++ I+ KD F KA GE ++
Sbjct: 325 GEDMNTQHLVLAQFDKVTRTKSRWKCSLKDGIMHINDKDILFNKAAGEFDF 375
>At1g07470 [K] KOG2652 RNA polymerase II transcription initiation factor TFIIA
large chain
Length = 397
Score = 50.1 bits (118), Expect = 3e-06
Identities = 22/50 (44%), Positives = 34/50 (68%), Gaps = 3/50 (6%)
Query: 182 GTDVN---MMLCLYDKVLRVKNKWKCNLKEGVVTIDHKDFAFQKAQGESE 228
G D+N ++L +DKV R K++WKC+LK+G++ I+ KD F KA S+
Sbjct: 325 GEDMNTQHLVLAQFDKVTRTKSRWKCSLKDGIMHINDKDILFNKASSTSD 374
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.308 0.127 0.358
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,471,672
Number of Sequences: 60738
Number of extensions: 414115
Number of successful extensions: 1401
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1383
Number of HSP's gapped (non-prelim): 18
length of query: 229
length of database: 30,389,216
effective HSP length: 103
effective length of query: 126
effective length of database: 24,133,202
effective search space: 3040783452
effective search space used: 3040783452
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)