ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI0217 good H KOG2672 Coenzyme transport and metabolism Lipoate synthase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI0217 75044 73935 -370
(370 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR196c [H] KOG2672 Lipoate synthase 553 e-157
SPBC8D2.15 [H] KOG2672 Lipoate synthase 429 e-120
7296306 [H] KOG2672 Lipoate synthase 400 e-111
Hs20535296 [H] KOG2672 Lipoate synthase 377 e-104
CE01031 [H] KOG2672 Lipoate synthase 365 e-101
At2g20860 [H] KOG2672 Lipoate synthase 341 1e-93
At5g08410 [H] KOG2672 Lipoate synthase 294 2e-79
SPBPI050 [H] KOG2672 Lipoate synthase 259 3e-69
>YOR196c [H] KOG2672 Lipoate synthase
Length = 414
Score = 553 bits (1426), Expect = e-157
Identities = 267/338 (78%), Positives = 293/338 (85%)
Query: 29 RRKTTHFTDKLNKGPSFEDFVNGNAQKFTLDPLEKARQNSEEVQRLPTWLKVPIAKGSNF 88
R++ T F D LN GPSF DFV+G A K LDPLEKARQN+EE ++LP WLKVPI KG+N+
Sbjct: 75 RKRITEFKDALNLGPSFADFVSGKASKMILDPLEKARQNTEEAKKLPRWLKVPIPKGTNY 134
Query: 89 HKLKNDVKELKLSTVCEEAKCPNIGECWGGGDKSKATATIMLLGDTCTRGCRFCSVKTNR 148
HKLK DVKEL LSTVCEEA+CPNIGECWGG DKSKATATIMLLGDTCTRGCRFCSVKTNR
Sbjct: 135 HKLKGDVKELGLSTVCEEARCPNIGECWGGKDKSKATATIMLLGDTCTRGCRFCSVKTNR 194
Query: 149 KPSAPDPTEPENTAEAISRWGLGYXXXXXXXXXXXXXGGAYHLAETVQKIKQKAPNILVE 208
PS PDP EPENTAEAI RWGLGY GGA HLAETV+KIKQKAPN LVE
Sbjct: 195 TPSKPDPMEPENTAEAIKRWGLGYVVLTTVDRDDLVDGGANHLAETVRKIKQKAPNTLVE 254
Query: 209 TLSGDFRGDLEMVKVMALSGLDVYAHNMETVEALTPHVRDRRATYRQSLSVLECAKKTVP 268
TLSGDFRGDL+MV +MA GLDVYAHN+ETVE+LTPHVRDRRATYRQSLSVLE AK TVP
Sbjct: 255 TLSGDFRGDLKMVDIMAQCGLDVYAHNLETVESLTPHVRDRRATYRQSLSVLERAKATVP 314
Query: 269 TLVTKTSVMLGLGETDEQVLQTMKDLRAIGCDVITFGQYMRPTKRHMKVVEYVTPEKFDY 328
+L+TKTS+MLGLGETDEQ+ QT+KDLR I CDV+TFGQYMRPTKRHMKVVEYV PEKFDY
Sbjct: 315 SLITKTSIMLGLGETDEQITQTLKDLRNIQCDVVTFGQYMRPTKRHMKVVEYVKPEKFDY 374
Query: 329 WKQKALELGFLYCASGPLVRSSYKAGESFIENVLRGRS 366
WK++ALE+GFLYCASGPLVRSSYKAGE+FIENVL+ R+
Sbjct: 375 WKERALEMGFLYCASGPLVRSSYKAGEAFIENVLKKRN 412
>SPBC8D2.15 [H] KOG2672 Lipoate synthase
Length = 370
Score = 429 bits (1102), Expect = e-120
Identities = 212/354 (59%), Positives = 269/354 (75%), Gaps = 4/354 (1%)
Query: 17 TVVRSTQPSVSRRRKTTHFTDKLNKGPSFEDFVNGNAQKFTLDPLEKARQ----NSEEVQ 72
T++++ S R ++ F++KL KGPSF DF+N + + E R+ N +
Sbjct: 9 TILKNNFWFASVRFQSGGFSEKLAKGPSFADFLNMDKPLTADEAFELDRKVELPNGSIHK 68
Query: 73 RLPTWLKVPIAKGSNFHKLKNDVKELKLSTVCEEAKCPNIGECWGGGDKSKATATIMLLG 132
RLP+WLK + G+NF+++K+D++ L TVCEEAKCPNIGECWGG DKS+ATATIML+G
Sbjct: 69 RLPSWLKTKVPLGTNFNRIKHDLRGSHLHTVCEEAKCPNIGECWGGKDKSRATATIMLMG 128
Query: 133 DTCTRGCRFCSVKTNRKPSAPDPTEPENTAEAISRWGLGYXXXXXXXXXXXXXGGAYHLA 192
DTCTRGCRFCSVKT+R+P DP EPENTAEAI +W LGY GA H+A
Sbjct: 129 DTCTRGCRFCSVKTSRRPGPLDPNEPENTAEAIKQWNLGYIVLTSVDRDDLTDLGANHIA 188
Query: 193 ETVQKIKQKAPNILVETLSGDFRGDLEMVKVMALSGLDVYAHNMETVEALTPHVRDRRAT 252
+T+QKIK+KAP+ILVE L+ DF G +++V+++A SGLDV+AHN+ETVE LTP VRDRRAT
Sbjct: 189 KTIQKIKEKAPHILVEALTPDFSGRMDLVEIVAKSGLDVFAHNVETVEELTPFVRDRRAT 248
Query: 253 YRQSLSVLECAKKTVPTLVTKTSVMLGLGETDEQVLQTMKDLRAIGCDVITFGQYMRPTK 312
YRQSLSVL+ KKT P L+TKTS+MLGLGETD ++L T+KDL DV+TFGQYMRPTK
Sbjct: 249 YRQSLSVLKHVKKTCPHLITKTSIMLGLGETDAEILTTLKDLLEHNVDVVTFGQYMRPTK 308
Query: 313 RHMKVVEYVTPEKFDYWKQKALELGFLYCASGPLVRSSYKAGESFIENVLRGRS 366
RH+KV EYV P+KF+YWK+ A +LGFLY ASGPLVRSSYKAGE F+EN+++ RS
Sbjct: 309 RHLKVQEYVHPKKFEYWKEVAEKLGFLYVASGPLVRSSYKAGEYFMENLIKKRS 362
>7296306 [H] KOG2672 Lipoate synthase
Length = 380
Score = 400 bits (1028), Expect = e-111
Identities = 200/354 (56%), Positives = 249/354 (69%), Gaps = 4/354 (1%)
Query: 18 VVRSTQPSVSRRRKTTHFTDKLNKGPSFEDFV----NGNAQKFTLDPLEKARQNSEEVQR 73
+V +T+ + + K ++L KGP+F DFV N + D + + E+ R
Sbjct: 16 IVVATRAASTNAEKLEEIRERLAKGPNFHDFVQNPDNTRNEWEQYDGKLRREKGEEQRLR 75
Query: 74 LPTWLKVPIAKGSNFHKLKNDVKELKLSTVCEEAKCPNIGECWGGGDKSKATATIMLLGD 133
LP WLK I G N+ K+K ++ELKLSTVCEEA+CPNIGECWGGG+ TATIML+GD
Sbjct: 76 LPPWLKTTIPVGKNYAKIKAQMRELKLSTVCEEARCPNIGECWGGGEHGTQTATIMLMGD 135
Query: 134 TCTRGCRFCSVKTNRKPSAPDPTEPENTAEAISRWGLGYXXXXXXXXXXXXXGGAYHLAE 193
TCTRGCRFCSVKT RKP D EP NTA AI+ WGL Y GG+ H+AE
Sbjct: 136 TCTRGCRFCSVKTARKPPPLDVNEPVNTATAIASWGLDYIVLTSVDRDDLPDGGSEHIAE 195
Query: 194 TVQKIKQKAPNILVETLSGDFRGDLEMVKVMALSGLDVYAHNMETVEALTPHVRDRRATY 253
TV++IK + NI VE L DFRG+LE VK +A SGLDVYAHN+ETVE LTP+VRDRRA Y
Sbjct: 196 TVREIKARNSNIFVECLVPDFRGNLECVKTIANSGLDVYAHNIETVEKLTPYVRDRRAHY 255
Query: 254 RQSLSVLECAKKTVPTLVTKTSVMLGLGETDEQVLQTMKDLRAIGCDVITFGQYMRPTKR 313
RQ+L VL AK+ P L+TK+S+MLGLGETDE++ T+KDLR G D +T GQYM+PT +
Sbjct: 256 RQTLQVLTEAKRFNPNLITKSSIMLGLGETDEEIENTLKDLREAGVDCVTLGQYMQPTNK 315
Query: 314 HMKVVEYVTPEKFDYWKQKALELGFLYCASGPLVRSSYKAGESFIENVLRGRSR 367
H+KV+EYVTPEKF +W+++ ELGFLY ASGPLVRSSYKAGE FI ++L R +
Sbjct: 316 HLKVIEYVTPEKFKHWEERGNELGFLYTASGPLVRSSYKAGEFFITSILENRKK 369
>Hs20535296 [H] KOG2672 Lipoate synthase
Length = 372
Score = 377 bits (968), Expect = e-104
Identities = 191/330 (57%), Positives = 236/330 (70%), Gaps = 4/330 (1%)
Query: 39 LNKGPSFEDFVNGN-AQKFTLDPLEK--ARQNSEEVQRLPTWLKVPIAKGSNFHKLKNDV 95
L GP +DFV+G+ A + T D + RQ E + RLP WLK I G N++KLKN +
Sbjct: 39 LQNGPDLQDFVSGDLADRSTWDEYKGNLKRQKGERL-RLPPWLKTEIPMGKNYNKLKNTL 97
Query: 96 KELKLSTVCEEAKCPNIGECWGGGDKSKATATIMLLGDTCTRGCRFCSVKTNRKPSAPDP 155
+ L L TVCEEA+CPNIGECWGGG+ + ATATIML+GDTCTRGCRFCSVKT R P D
Sbjct: 98 RNLNLHTVCEEARCPNIGECWGGGEYATATATIMLMGDTCTRGCRFCSVKTARNPPPLDA 157
Query: 156 TEPENTAEAISRWGLGYXXXXXXXXXXXXXGGAYHLAETVQKIKQKAPNILVETLSGDFR 215
+EP NTA+AI+ WGL Y GGA H+A+TV +K++ P ILVE L+ DFR
Sbjct: 158 SEPYNTAKAIAEWGLDYVVLTSVDRDDMPDGGAEHIAKTVSYLKERNPKILVECLTPDFR 217
Query: 216 GDLEMVKVMALSGLDVYAHNMETVEALTPHVRDRRATYRQSLSVLECAKKTVPTLVTKTS 275
GDL+ ++ +ALSGLDVYAHN+ETV L VRD RA + QSL VL+ AKK P +++KTS
Sbjct: 218 GDLKAIEKVALSGLDVYAHNVETVPELQSKVRDPRANFDQSLRVLKHAKKVQPDVISKTS 277
Query: 276 VMLGLGETDEQVLQTMKDLRAIGCDVITFGQYMRPTKRHMKVVEYVTPEKFDYWKQKALE 335
+MLGLGE DEQV TMK LR D +T GQYM+PT+RH+KV EY+TPEKF YW++ E
Sbjct: 278 IMLGLGENDEQVYATMKALREADVDCLTLGQYMQPTRRHLKVEEYITPEKFKYWEKVGNE 337
Query: 336 LGFLYCASGPLVRSSYKAGESFIENVLRGR 365
LGF Y ASGPLVRSSYKAGE F++N++ R
Sbjct: 338 LGFHYTASGPLVRSSYKAGEFFLKNLVAKR 367
>CE01031 [H] KOG2672 Lipoate synthase
Length = 354
Score = 365 bits (937), Expect = e-101
Identities = 183/329 (55%), Positives = 230/329 (69%), Gaps = 5/329 (1%)
Query: 42 GPSFEDFVNGNAQKFTLDPLEKAR--QNSEEVQRLPTWLK---VPIAKGSNFHKLKNDVK 96
GPS +DF++ + ++ E + + RLP WLK + ++ N +LK +K
Sbjct: 24 GPSLQDFISSASVAEAVEKYEGKLKLEKGDRRLRLPPWLKKEKILPSENENVSRLKKQLK 83
Query: 97 ELKLSTVCEEAKCPNIGECWGGGDKSKATATIMLLGDTCTRGCRFCSVKTNRKPSAPDPT 156
LKL+TVC+EA+CPN+GECWGG D S ATATIML+GDTCTRGC+FCSVKT R P DP
Sbjct: 84 HLKLATVCQEARCPNLGECWGGSDDSLATATIMLMGDTCTRGCKFCSVKTARAPPPLDPM 143
Query: 157 EPENTAEAISRWGLGYXXXXXXXXXXXXXGGAYHLAETVQKIKQKAPNILVETLSGDFRG 216
EPENT+ A++ WG+ Y GGA HL +TVQ +K K P +L+E L DF G
Sbjct: 144 EPENTSTAVASWGVEYIVLTSVDRDDLPDGGADHLRKTVQLMKLKKPELLIECLLPDFAG 203
Query: 217 DLEMVKVMALSGLDVYAHNMETVEALTPHVRDRRATYRQSLSVLECAKKTVPTLVTKTSV 276
D V+ MA SGLDVYAHN+ETVE LTP VRD RA YRQSL L AK+ P L+TKTS+
Sbjct: 204 DKISVEKMATSGLDVYAHNIETVERLTPWVRDPRAKYRQSLDALRYAKEVSPKLITKTSI 263
Query: 277 MLGLGETDEQVLQTMKDLRAIGCDVITFGQYMRPTKRHMKVVEYVTPEKFDYWKQKALEL 336
MLGLGE ++++ Q + DLRA DV+TFGQYM+PTKRH+ V E+VTPEKFD W + + +L
Sbjct: 264 MLGLGEAEDEIKQCLADLRASNVDVVTFGQYMQPTKRHLLVKEWVTPEKFDQWAEYSKKL 323
Query: 337 GFLYCASGPLVRSSYKAGESFIENVLRGR 365
GFLY ASGPLVRSSYKAGE +++NVLR R
Sbjct: 324 GFLYVASGPLVRSSYKAGEFYLKNVLRNR 352
>At2g20860 [H] KOG2672 Lipoate synthase
Length = 374
Score = 341 bits (874), Expect = 1e-93
Identities = 169/324 (52%), Positives = 221/324 (68%), Gaps = 8/324 (2%)
Query: 40 NKGPSFEDFVNGNAQKFTLDPLEKARQNSEEVQRLPTWLKVPIAKGSNFHKLKNDVKELK 99
N+ PS DF++G+ + +K P W+K I G + ++K +++LK
Sbjct: 46 NESPSLTDFIHGDTYSVEVGTKKKPLPK-------PKWMKESIPGGERYVQIKKKLRDLK 98
Query: 100 LSTVCEEAKCPNIGECWGGGDKSKATATIMLLGDTCTRGCRFCSVKTNRKPSAPDPTEPE 159
L TVCEEAKCPN+GECW GG+ ATATIM+LGDTCTRGCRFC+VKT+R P PDP EP
Sbjct: 99 LHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPN 158
Query: 160 NTAEAISRWGLGYXXXXXXXXXXXXXGGAYHLAETVQKIKQKAPNILVETLSGDFRGDLE 219
N AEAI+ WG+ Y G+ H AETVQ++K P +L+E L DFRGD
Sbjct: 159 NVAEAIASWGVDYVVITSVDRDDLPDQGSGHFAETVQRLKFLKPEMLIEALVPDFRGDGG 218
Query: 220 MVKVMALSGLDVYAHNMETVEALTPHVRDRRATYRQSLSVLECAKKTVPT-LVTKTSVML 278
V+ ++ SGLDV AHN+ETVE L VRD RA ++QSL VL AK+ P +TKTSVML
Sbjct: 219 CVEKVSKSGLDVLAHNIETVEELQSFVRDHRANFKQSLDVLRMAKEYAPAGTLTKTSVML 278
Query: 279 GLGETDEQVLQTMKDLRAIGCDVITFGQYMRPTKRHMKVVEYVTPEKFDYWKQKALELGF 338
G GET +QV++TM+ +RA G DV+TFGQYMRP+KRHM V EYVTP+ F+ ++ +E+GF
Sbjct: 279 GCGETPDQVVKTMEKVRAAGVDVMTFGQYMRPSKRHMPVAEYVTPDAFERYRLLGMEMGF 338
Query: 339 LYCASGPLVRSSYKAGESFIENVL 362
Y ASGP+VRSSYKAGE +I++++
Sbjct: 339 RYVASGPMVRSSYKAGEYYIKSMI 362
>At5g08410 [H] KOG2672 Lipoate synthase
Length = 477
Score = 294 bits (752), Expect = 2e-79
Identities = 146/287 (50%), Positives = 186/287 (63%), Gaps = 20/287 (6%)
Query: 75 PTWLKVPIAKGSNFHKLKNDVKELKLSTVCEEAKCPNIGECWGGGDKSKATATIMLLGDT 134
P WL+ +G F ++K + L L+TVCEEA+CPNIGECW GG ATATIM+LGDT
Sbjct: 42 PAWLRQKAPQGERFQEVKESLSRLNLNTVCEEAQCPNIGECWNGGGDGVATATIMVLGDT 101
Query: 135 CTRGCRFCSVKTNRKPSAPDPTEPENTAEAISRWGLGYXXXXXXXXXXXXXGGAYHLAET 194
CTRGCRFC+VKT+R P PDP EPENTA+AI+ WG+ Y GG+ H A+T
Sbjct: 102 CTRGCRFCAVKTSRNPPPPDPMEPENTAKAIASWGVDYIVITSVDRDDIPDGGSGHFAQT 161
Query: 195 VQKIKQKAPNILVETLSGDFRGDLEMVKVMALSGLDVYAHNMETVEALTPHVRDRRATYR 254
V+ +K V + SGLDV+AHN+ETV+ L VRD RA Y
Sbjct: 162 VKAMKA--------------------VDTLVHSGLDVFAHNVETVKRLQRLVRDPRAGYE 201
Query: 255 QSLSVLECAKKTVPTLVTKTSVMLGLGETDEQVLQTMKDLRAIGCDVITFGQYMRPTKRH 314
QS+SVL+ AK + P ++TKTS+MLGLGETDE++ + M DLRAI D++T GQY++PT H
Sbjct: 202 QSMSVLKHAKISKPGMITKTSIMLGLGETDEELKEAMADLRAIDVDILTLGQYLQPTPLH 261
Query: 315 MKVVEYVTPEKFDYWKQKALELGFLYCASGPLVRSSYKAGESFIENV 361
+ V EYVTPEKFD+WK +GF Y ASGPL+ +SY + I V
Sbjct: 262 LTVKEYVTPEKFDFWKTYGESIGFRYVASGPLMTNSYALSPAIIAAV 308
>SPBPI050 [H] KOG2672 Lipoate synthase
Length = 244
Score = 259 bits (663), Expect = 3e-69
Identities = 128/236 (54%), Positives = 169/236 (71%), Gaps = 4/236 (1%)
Query: 17 TVVRSTQPSVSRRRKTTHFTDKLNKGPSFEDFVNGNAQKFTLDPLEKARQ----NSEEVQ 72
T++++ S R ++ F++KL KGPSF DF+N + + E R+ N +
Sbjct: 9 TILKNNFWFASVRFQSGGFSEKLAKGPSFADFLNMDKPLTADEAFELDRKVELPNGSIHK 68
Query: 73 RLPTWLKVPIAKGSNFHKLKNDVKELKLSTVCEEAKCPNIGECWGGGDKSKATATIMLLG 132
RLP+WLK + G+NF+++K+D++ L TVCEEAKCPNIGECWGG DKS+ATATIML+G
Sbjct: 69 RLPSWLKTKVPLGTNFNRIKHDLRGSHLHTVCEEAKCPNIGECWGGKDKSRATATIMLMG 128
Query: 133 DTCTRGCRFCSVKTNRKPSAPDPTEPENTAEAISRWGLGYXXXXXXXXXXXXXGGAYHLA 192
DTCTRGCRFCSVKT+R+P DP EPENTAEAI +W LGY GA H+A
Sbjct: 129 DTCTRGCRFCSVKTSRRPGPLDPNEPENTAEAIKQWNLGYIVLTSVDRDDLTDLGANHIA 188
Query: 193 ETVQKIKQKAPNILVETLSGDFRGDLEMVKVMALSGLDVYAHNMETVEALTPHVRD 248
+T+QKIK+KAP+ILVE L+ DF G +++V+++A SGLDV+AHN+ETVE LTP VRD
Sbjct: 189 KTIQKIKEKAPHILVEALTPDFSGRMDLVEIVAKSGLDVFAHNVETVEELTPFVRD 244
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.133 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,932,433
Number of Sequences: 60738
Number of extensions: 838738
Number of successful extensions: 2111
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2096
Number of HSP's gapped (non-prelim): 8
length of query: 370
length of database: 30,389,216
effective HSP length: 108
effective length of query: 262
effective length of database: 23,829,512
effective search space: 6243332144
effective search space used: 6243332144
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)