ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI0265 good A KOG0838 RNA processing and modification RNA Methylase, SpoU family
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI0265 87897 86698 -400
(400 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR201c [A] KOG0838 RNA Methylase SpoU family 311 1e-84
SPBC1347.13c [A] KOG0838 RNA Methylase SpoU family 115 9e-26
At2g19870 [A] KOG0838 RNA Methylase SpoU family 95 2e-19
CE19170 [A] KOG0838 RNA Methylase SpoU family 62 1e-09
>YOR201c [A] KOG0838 RNA Methylase SpoU family
Length = 412
Score = 311 bits (796), Expect = 1e-84
Identities = 172/388 (44%), Positives = 243/388 (62%), Gaps = 18/388 (4%)
Query: 25 IKDRKVTFDRNFPV--HKRTKAWEKDGMDKDKWFRKNYAHVHAREKNARLNNGGSDPFDK 82
IK + +FD+ FP + R K WE DK WF++ YAHVHARE++ +DP+ K
Sbjct: 27 IKKKSSSFDKFFPQQSNSRKKQWETLNEDKASWFKRKYAHVHAREQDR-----AADPYGK 81
Query: 83 KKKYYDRIKQEESSKREQFRSHRSEY-TKMHPLQGLKPNPLVDYVFGTNSVISALTAKKR 141
KK + +++K+ ++ + +SH+S++ K L+ + NP+ +YV+GTNSV +AL R
Sbjct: 82 KKAHVEKLKEIKNQAKLNQKSHKSKFQNKDIALKLMNDNPIFEYVYGTNSVYAALLNPSR 141
Query: 142 EYFVKLHHYGPLNTQIQSLCSKNNIQTEEVDKHRLNLYTNYAVHNNVVLECKPLQPPELS 201
+L ++G + ++ + + + TE VDKHRLNL TNY VHNN+ LE KPLQP E++
Sbjct: 142 NCHSRLLYHGTIPSKFLQIVDELKVTTELVDKHRLNLLTNYGVHNNIALETKPLQPVEIA 201
Query: 202 HLGLVNHEACTFDKSEIDYDQAVNTREK--FMTNDNKKNPLGIYLDEVVDPHNVGAIIRS 259
+LG ++ + E+ ++ E D KK PLG+YLDE+ DPHN+GAIIRS
Sbjct: 202 YLGDMDTSSAALSIHELGFNNENIPHELPYGTKTDAKKFPLGLYLDEITDPHNIGAIIRS 261
Query: 260 AYFLGADFMVLSHKNCAPLSPVVSKVSSGTMELLPIFTTAKPLQFFEKSKQDGGWMFVTS 319
AYFLG DF+V+S +NC+PL+PVVSK SSG +ELLPIF KPL+FF KS++ GGW F+TS
Sbjct: 262 AYFLGVDFIVMSRRNCSPLTPVVSKTSSGALELLPIFYVDKPLEFFTKSQEMGGWTFITS 321
Query: 320 HM--SISQK---GNHLTMNELSGMXXXXXXXXXXXXXXXXXRTNLLNRSDFTVEIPFGRD 374
H+ + S+K G ++M++L+G+ RTNL RSDF VEIPFG
Sbjct: 322 HLANATSEKYTVGKTISMHDLNGLCNELPVVLVVGNESQGVRTNLKMRSDFFVEIPFGGI 381
Query: 375 PTEGR---NTIDSLNVSVATALLINGFL 399
R +DSLNVSVATALLI+ L
Sbjct: 382 EKGNRAPEPIVDSLNVSVATALLIDNIL 409
>SPBC1347.13c [A] KOG0838 RNA Methylase SpoU family
Length = 301
Score = 115 bits (289), Expect = 9e-26
Identities = 88/279 (31%), Positives = 130/279 (46%), Gaps = 11/279 (3%)
Query: 124 DYVFGTNSVISALTAKKREYFVKLHHYGPLNTQIQSLCSK--NNIQTEEVDKHRLNLYTN 181
++VFGT+SV +AL +KRE ++++ + +K I + V+K LN T
Sbjct: 32 EFVFGTHSVKNALATRKRECRALYVQNADIHSEFEEFLNKLQYKIPIKSVNKEHLNQITA 91
Query: 182 YAVHNNVVLECKPLQPPELSHLGLVNHEACTFDKSEIDYDQAVNTREKFMTNDNKKNPLG 241
HN VVLE L P +S L E +K+ D K ++DN PL
Sbjct: 92 CRPHNGVVLEASSLNVPTISDLLFPAGEEYN-NKNGQDSPHNDLNEGKSSSSDNYP-PLY 149
Query: 242 IYLDEVVDPHNVGAIIRSAYFLGADFMVLSHKNCAPLSPVVSKVSSGTMELLPIFTTAKP 301
+Y+D + DP N+GA+IRSAY LGA ++LS K+ LSPVVSK S+G +E+ I P
Sbjct: 150 VYVDGITDPQNMGAVIRSAYILGAKGILLSKKHNTFLSPVVSKASAGALEVFNISHVKNP 209
Query: 302 LQFFEKSKQDGGWMFVTSHMSISQKGNHLTMNELSGMXXXXXXXXXXXXXXXXXRTNLLN 361
+ F S GW + + ++ + + RTN+L
Sbjct: 210 MVFLRNSVLK-GWKVIGTKPALPDNKDQIYTPHKIKTELMNEPKILVLGSEKGLRTNILT 268
Query: 362 RSDFTVEIPFGRDPTEGRNTIDSLNVSVATALLINGFLN 400
+ V IP G +DSLNVSVA +L+ +N
Sbjct: 269 QCSHYVSIP------GGDKYVDSLNVSVAAGILLYSLVN 301
>At2g19870 [A] KOG0838 RNA Methylase SpoU family
Length = 577
Score = 94.7 bits (234), Expect = 2e-19
Identities = 64/212 (30%), Positives = 104/212 (48%), Gaps = 39/212 (18%)
Query: 124 DYVFGTNSVISALTAKKREYFVKLHHYG-PLNT------------QIQSLCSKNNIQTEE 170
+ V+G + V++AL+ +RE++ G L++ ++ + K + +E
Sbjct: 302 EVVYGVSPVLAALSVGRREFYALYVQEGLDLSSNNRKKKDKKGFERVLKISEKLGLNIKE 361
Query: 171 VDKHRLNLYTNYAVHNNVVLECKPLQPPELSHLGLVNHEACTFDKSEIDYDQAVNTREKF 230
KH LN+ + H +VL+ PL+ ++ L ++ E
Sbjct: 362 TSKHDLNMVADNRPHQGLVLDASPLELVKVKELDPISSE--------------------- 400
Query: 231 MTNDNKKNPLGIYLDEVVDPHNVGAIIRSAYFLGADFMVLSHKNCAPLSPVVSKVSSGTM 290
+K L + LDEV DP N+GAIIRSAYF GA +V+ KN APLS VVSK S+G++
Sbjct: 401 ----EEKYSLWVALDEVTDPQNLGAIIRSAYFFGATGVVVCAKNSAPLSAVVSKASAGSL 456
Query: 291 ELLPIFTTAKPLQFFEKSKQDGGWMFVTSHMS 322
E++ + +QF E S ++ GW V +S
Sbjct: 457 EVMELRYCKNMMQFLEASAEN-GWRVVGGSVS 487
>CE19170 [A] KOG0838 RNA Methylase SpoU family
Length = 294
Score = 62.4 bits (150), Expect = 1e-09
Identities = 67/281 (23%), Positives = 122/281 (42%), Gaps = 50/281 (17%)
Query: 126 VFGTNSVISALTAKKREY---FVK--LHHYGPLNTQIQSLCSKNN---IQTEEVDKHRLN 177
+FG +SV+ A+ + KR++ F+K + + + +I+ + S+ + IQ + +L+
Sbjct: 45 LFGLHSVLEAIRSGKRDFHSVFLKKAVENRADSDARIKEILSRIDELGIQKTLLTDDQLD 104
Query: 178 LYTNYAVHNNVVLECKPLQPPELSHLGLVNHEACTFDKSEIDYDQAVNTREKFMTNDNKK 237
T + +HN + ++ LQ N E T
Sbjct: 105 RLTEFQLHNGICMDASALQ--------FSNFEPKT------------------------- 131
Query: 238 NPLGIYLDEVVDPHNVGAIIRSAYFLGADFM-VLSHKNCAPLSPVVSKVSSGTMELLPIF 296
+ I++D+V+DP N+GAI RSA++ GAD + ++ + ++P + K S G +E LP+
Sbjct: 132 --VSIFVDKVLDPGNLGAIGRSAWYFGADGLGLVKGRGPKSITPAMIKSSCGALEHLPVQ 189
Query: 297 TTAKPLQFFEKSKQDGGWMFVTSHMSISQKGNHLTMNELSGMXXXXXXXXXXXXXXXXXR 356
+F +K K GG + T ++K ++ L
Sbjct: 190 QFLNFNEFRDKIKAAGGELVATCDPGAAEKAG-ISAKSLDQWTPEKPVGIVLGDEGVGIS 248
Query: 357 TNLLNRSDFTVEI-PFGRDPTEGRNTIDSLNVSVATALLIN 396
+LN D + I P +D G N + SLNVSV +L++
Sbjct: 249 RGILNLCDTVISISPVRKD---GSN-VTSLNVSVVAGILLH 285
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.133 0.391
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,352,090
Number of Sequences: 60738
Number of extensions: 1063488
Number of successful extensions: 2884
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2868
Number of HSP's gapped (non-prelim): 6
length of query: 400
length of database: 30,389,216
effective HSP length: 109
effective length of query: 291
effective length of database: 23,768,774
effective search space: 6916713234
effective search space used: 6916713234
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)