ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI0319 good T KOG2751 Signal transduction mechanisms Beclin-like protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI0319 107298 108680 461
(461 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPL120w [T] KOG2751 Beclin-like protein 302 6e-82
Hs4502395 [T] KOG2751 Beclin-like protein 145 1e-34
7301093 [T] KOG2751 Beclin-like protein 139 7e-33
CE27592 [T] KOG2751 Beclin-like protein 76 9e-14
At3g61710 [T] KOG2751 Beclin-like protein 70 5e-12
>YPL120w [T] KOG2751 Beclin-like protein
Length = 557
Score = 302 bits (774), Expect = 6e-82
Identities = 183/541 (33%), Positives = 290/541 (52%), Gaps = 88/541 (16%)
Query: 6 LRCMHCHSMLEIDVSLMDLSMAQRDLLVSSDANTSGKDSSANSN-----------QIPAD 54
++C CH L++D SL LS+ QR+LL+S+++ + + + SN QIP +
Sbjct: 1 MKCQTCHLPLQLDPSLEGLSLTQRNLLLSNNSIITATNENVISNKGIEAADNCGPQIPKE 60
Query: 55 KLKVMKEVKQPSQLRLTKPGNIASDSYVFL------------------------------ 84
+L+ + E++ L L K + +DS+VFL
Sbjct: 61 RLRRLGEIQNIKDLNL-KDDKLITDSFVFLNHDDDDNANITSNSREDQRYGNANGNDNKK 119
Query: 85 --------TDTEYSLQKSKIXXXXXXXXXXXXXRNKTLSSRINALGNIFNILSSKNNVDY 136
T T + + +KTLS+++NA+ N+FNILSS+ N+D+
Sbjct: 120 ANSDTSDGTSTFRDHDEEEQEATDEDENQQIQLNSKTLSTQVNAMTNVFNILSSQTNIDF 179
Query: 137 PVCQGCCDTLLEKLKEEYNQELKKRDTYHDFMKRIQ-EHKNVNGINIGDNRAXXXXXXXX 195
P+CQ CC+ L+ +LK EY+ +K+RDTY F+ +++ ++K ++ N +
Sbjct: 180 PICQDCCNILINRLKSEYDDAIKERDTYAQFLSKLESQNKEISESNKEKQYSHNLSEKEN 239
Query: 196 XXXXXXXXXXXXXXXXXXXXQK--ETILLQEELAKKKDQYIVRLQKQNIQELEQLTFIKD 253
E + LQE+ + +++ + +L QN+ +L + F K+
Sbjct: 240 LKKEEERLLDQLLRLEMTDDDLDGELVRLQEKKVQLENEKLQKLSDQNLMDLNNIQFNKN 299
Query: 254 VQSLKNQRVVTLNHIDKLRKLNIYNETFRISHDGPFGTINDLKLGSVPNASVPWSEINSA 313
+QSLK Q ++LN +DKLRK+NI+N TF+ISH GPF TIN L+LGS+P + VPW EIN+A
Sbjct: 300 LQSLKLQYELSLNQLDKLRKINIFNATFKISHSGPFATINGLRLGSIPESVVPWKEINAA 359
Query: 314 LGQVVLLLSLIAEKLSVSFTDYKLIPMGSTSSIEK-------FDPKT----NQWFVHKAF 362
LGQ++LLL+ I + L ++ DY+L PMGS S I+K ++ T W + +
Sbjct: 360 LGQLILLLATINKNLKINLVDYELQPMGSFSKIKKRMVNSVEYNNSTTNAPGDWLILPVY 419
Query: 363 SGDEFSFGSLFHKESAIDKALTCILEIISLLSAKVS-----------SDSQDPAS----- 406
+ F+ G +F KE+ DK+L LEIIS ++ ++S + SQD +S
Sbjct: 420 YDENFNLGRIFRKETKFDKSLETTLEIISEITRQLSTIASSYSSQTLTTSQDESSMNNAN 479
Query: 407 --------IELPYEILGDKINGLTILLNGATPSLEWTTSCKFLLTNVKWLLAFSTAHIDK 458
+ELPY + DKINGL++ L+G++P+LEWTT+ KFLLTNVKWLLAFS+ + K
Sbjct: 480 DVENSTSILELPYIMNKDKINGLSVKLHGSSPNLEWTTAMKFLLTNVKWLLAFSSNLLSK 539
Query: 459 N 459
+
Sbjct: 540 S 540
>Hs4502395 [T] KOG2751 Beclin-like protein
Length = 450
Score = 145 bits (366), Expect = 1e-34
Identities = 95/349 (27%), Positives = 168/349 (47%), Gaps = 25/349 (7%)
Query: 112 KTLSSRINALGNIFNILSSKNNVDYPVCQGCCDTLLEKLKEEYNQELKKRDTYHDFMKRI 171
+ LS R+ G++F+I+S + +VD+P+C+ C DTLL++L + N + Y ++ +
Sbjct: 110 ENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTDTLLDQLDTQLNVTENECQNYKRCLEIL 169
Query: 172 QEHKNVNGINIGDNRAXXXXXXXXXXXXXXXXXXXXXXXXXXXXQKETILLQEELAKKKD 231
++ +N D+ E + + A++ D
Sbjct: 170 EQ------MNEDDSEQLQMELKELALEEERLIQELEDVEKNRKIVAENLEKVQAEAERLD 223
Query: 232 QYIVRLQKQNIQ-ELEQLTFIKDVQSLKNQRVVTLNHIDKLRKLNIYNETFRISHDGPFG 290
Q + Q++ + + +QL +++S++NQ +DKL+K N++N TF I H G FG
Sbjct: 224 QEEAQYQREYSEFKRQQLELDDELKSVENQMRYAQTQLDKLKKTNVFNATFHIWHSGQFG 283
Query: 291 TINDLKLGSVPNASVPWSEINSALGQVVLLLSLIAEKLSVSFTDYKLIPMGSTSSIEKFD 350
TIN+ +LG +P+ V W+EIN+A GQ VLLL +A K+ + F Y+L+P G+ S +E
Sbjct: 284 TINNFRLGRLPSVPVEWNEINAAWGQTVLLLHALANKMGLKFQRYRLVPYGNHSYLESLT 343
Query: 351 PKTNQWFVHKAFSGDEFSFGSLFHKESAIDKALTCILEIISLLSAKVSSDSQDPASIELP 410
K+ + ++ S G F ++ D A+ L+ + +V + LP
Sbjct: 344 DKSKELPLY-------CSGGLRFFWDNKFDHAMVAFLDCVQQFKEEV---EKGETRFCLP 393
Query: 411 YEILGDK--------INGLTILLNGATPSLEWTTSCKFLLTNVKWLLAF 451
Y + +K G + +WT + KF+LTN+KW LA+
Sbjct: 394 YRMDVEKGKIEDTGGSGGSYSIKTQFNSEEQWTKALKFMLTNLKWGLAW 442
>7301093 [T] KOG2751 Beclin-like protein
Length = 422
Score = 139 bits (351), Expect = 7e-33
Identities = 97/347 (27%), Positives = 161/347 (45%), Gaps = 22/347 (6%)
Query: 111 NKTLSSRINALGNIFNILSSKNNVDYPVCQGCCDTLLEKLKEEYNQELKKRDTYHDFMKR 170
NK +S+ +F+ LSS + +D+P+C+ C D++LE + E + D Y ++
Sbjct: 84 NKKMSAAFKLKAELFDCLSSNSEIDHPLCEECADSMLEIMDRELRIAEDEWDVYKAYLDE 143
Query: 171 IQEHKNVNGINIGDNRAXXXXXXXXXXXXXXXXXXXXXXXXXXXXQKETILLQEELAKKK 230
+++ + + D +E +EEL +++
Sbjct: 144 LEQQRVAPNVEALDKELDELKRSEQQLLSELKELKKEEQSLNDAIAEEE-QEREELHEQE 202
Query: 231 DQYIVRLQKQNIQELEQLTFIKDVQSLKNQRVVTLNHIDKLRKLNIYNETFRISHDGPFG 290
+ Y K E + D +SL+ Q + +DKLR NI+N TF I H G FG
Sbjct: 203 ESYWREYTKHR---RELMLTEDDKRSLECQIAYSKQQLDKLRDTNIFNITFHIWHAGHFG 259
Query: 291 TINDLKLGSVPNASVPWSEINSALGQVVLLLSLIAEKLSVSFTDYKLIPMGSTSSIEKFD 350
TIN+ +LG +P+ SV WSEIN+A GQ VLLLS +A K+ ++F Y+++P G+ S +E
Sbjct: 260 TINNFRLGRLPSVSVDWSEINAAWGQTVLLLSALARKIGLTFERYRVVPFGNHSYVEVLG 319
Query: 351 PKTNQWFVHKAFSGDEFSFGSLFHKESAIDKALTCILEIISLLSAKVSSDSQDPASIELP 410
S G F ++ D A+ L+ ++ +V + LP
Sbjct: 320 ENRELPLYG--------SGGFKFFWDTKFDAAMVAFLDCLTQFQKEVEKRDTE---FLLP 368
Query: 411 YEILGDKI------NGLTILLNGATPSLEWTTSCKFLLTNVKWLLAF 451
Y++ KI N +I + + +WT + KF+LTN+KW LA+
Sbjct: 369 YKMEKGKIIDPSTGNSYSIKIQFNSEE-QWTKALKFMLTNLKWGLAW 414
>CE27592 [T] KOG2751 Beclin-like protein
Length = 375
Score = 76.3 bits (186), Expect = 9e-14
Identities = 74/322 (22%), Positives = 136/322 (41%), Gaps = 30/322 (9%)
Query: 135 DYPVCQGCCDTLLEKLKEEY---NQELKKRDTYHDFMKRIQEHKNVNGINIGDNRAXXXX 191
D PVC C D L ++ + + E+K TY +++K +N +I D +A
Sbjct: 61 DAPVCNDCSDALRNEMDAQVATLDDEIKTYQTYINYLK-----ENHPTTSIPDLKAKLQN 115
Query: 192 XXXXXXXXXXXXXXXXXXXXXXXXQKETILLQEELAKKKDQYIVRLQKQNIQELEQLTFI 251
+T E A +K + + + N++++ +
Sbjct: 116 VSDEEKELEQQLKKLLAEEEQLDLDLQTKRRTAEAASEKSGELWKKYRDNLRQVFEDQ-- 173
Query: 252 KDVQSLKNQRVVTLNHIDKLRKLNIYNETFRISHDGPFGTINDLKLGSVPNASVPWSEIN 311
++ SL+ +R KL N+ + F I DG G IN +LG + +A V ++EIN
Sbjct: 174 DELHSLEAERQYAEVQHRKLTDTNVLDLCFHIWVDGIVGEINGFRLGYLKDAPVEFTEIN 233
Query: 312 SALGQVVLLLSLIAEKLSVSFTDYKLIPMGSTSSIE----KFDPKTNQWFVHKAFSGDEF 367
+ALGQ+VLLL ++ E++ V + + MGS S I+ D +T +
Sbjct: 234 AALGQIVLLLEILLERIGVQHHELMPVAMGSHSYIKLRRNGIDMETYAL----------Y 283
Query: 368 SFGSLFHKESAIDKALTCILEIISLLSAKVSSDSQDPASIELPYEILGDKINGLTILLNG 427
G+ S ID + L+++ L ++ ++ + + PY+I D + + N
Sbjct: 284 GQGTPLSGSSGIDPGIRRFLQLLEFLLKELKDRNK---NFKPPYQIHADSLVDNGVKYNA 340
Query: 428 A---TPSLEWTTSCKFLLTNVK 446
+ WT + +LT++K
Sbjct: 341 VMTLNTDVRWTRAMALMLTDLK 362
>At3g61710 [T] KOG2751 Beclin-like protein
Length = 443
Score = 70.5 bits (171), Expect = 5e-12
Identities = 51/214 (23%), Positives = 88/214 (40%), Gaps = 17/214 (7%)
Query: 115 SSRINALGNIFNILSSKNNVDYPVCQGCCDTLLEKLKEEYNQELKKRDTYHDFMKRIQEH 174
++ IN L F+I ++ V+ P+C C L +KL++E + + Y ++R++
Sbjct: 148 NATINVLTRAFDIARTQTQVEQPLCLECMRVLSDKLEKEVEDVTRDVEAYEACVQRLE-- 205
Query: 175 KNVNGINIGDNRAXXXXXXXXXXXXXXXXXXXXXXXXXXXXQKETILLQEELAKK--KDQ 232
G+ + +K+ + +L + K
Sbjct: 206 --------GETQDVLSEADFLKEKKKIEEEERKLVAAIEETEKQNAEVNHQLKELEFKGN 257
Query: 233 YIVRLQKQNIQELEQLTFIKDVQSLKNQRV-----VTLNHIDKLRKLNIYNETFRISHDG 287
L+ + QE F + + V+ H++ L K N+ + F I +DG
Sbjct: 258 RFNELEDRYWQEFNNFQFQLIAHQEERDAILAKIEVSQAHLELLNKTNVLIDAFPIRNDG 317
Query: 288 PFGTINDLKLGSVPNASVPWSEINSALGQVVLLL 321
FGTIN+ +LG +P V W EIN+A GQ LLL
Sbjct: 318 EFGTINNFRLGRLPAIKVEWDEINAAWGQACLLL 351
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.132 0.370
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,655,798
Number of Sequences: 60738
Number of extensions: 911013
Number of successful extensions: 3284
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3269
Number of HSP's gapped (non-prelim): 10
length of query: 461
length of database: 30,389,216
effective HSP length: 110
effective length of query: 351
effective length of database: 23,708,036
effective search space: 8321520636
effective search space used: 8321520636
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)