ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI0432 good R KOG3880 General function prediction only Predicted small molecule transporter involved in cellular pH homeostasis (Batten disease protein in human)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI0432 147874 146714 -387
(387 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJL059w [R] KOG3880 Predicted small molecule transporter involve... 401 e-111
SPAC607.09c [R] KOG3880 Predicted small molecule transporter inv... 239 4e-63
CE09198 [R] KOG3880 Predicted small molecule transporter involve... 191 1e-48
Hs4502889 [R] KOG3880 Predicted small molecule transporter invol... 186 7e-47
CE07802 [R] KOG3880 Predicted small molecule transporter involve... 177 2e-44
CE27645 [R] KOG3880 Predicted small molecule transporter involve... 176 5e-44
7293939 [R] KOG3880 Predicted small molecule transporter involve... 163 4e-40
>YJL059w [R] KOG3880 Predicted small molecule transporter involved in
cellular pH homeostasis (Batten disease protein in
human)
Length = 408
Score = 401 bits (1030), Expect = e-111
Identities = 211/406 (51%), Positives = 272/406 (66%), Gaps = 22/406 (5%)
Query: 4 DRDKKTFAYFWLFGLINNILYVVILSAASDIIGPSLPKSIVLLFDIMPSFLIKLSAPFFV 63
D+ + + YFWLFGLINN+LYVVILSAA DI+GP+LPKS+VLL DI PS IKL +PFF+
Sbjct: 3 DKSHQIYCYFWLFGLINNVLYVVILSAAVDIVGPTLPKSLVLLADIFPSLAIKLCSPFFI 62
Query: 64 HSIHYDKRXXXXXXXXXXXXXXVSTRSLWLCLPGIVLASLSSGFGEITFLQLTHFFGSKS 123
I Y R VS ++L++CL GI AS+SSGFGE+TFLQLTH++ S
Sbjct: 63 DRIKYSYRIWSLITMSCLGMFLVSFKNLFVCLLGISFASISSGFGEVTFLQLTHYYKQIS 122
Query: 124 LTGWSSGTGGAGIVGSFSYLLLTTVFRXXXXXXXXXXXXXXXXXXXXYKI--NDSIVSSQ 181
L GWSSGTGGAGI+G SY+ LT++F+ +K+ ND+ ++ Q
Sbjct: 123 LNGWSSGTGGAGIIGGASYMFLTSIFKVPVKLTLLVFSLLPFAFLFYFKLESNDTNLTYQ 182
Query: 182 VYQSLDMPIDSS--DGPIELLKSNGWVDI----------VKRLSKLVIPYMIPLSTVYLF 229
Q +D D P+ +N + VKRL +LV PYM+PL+TVYLF
Sbjct: 183 SLQQIDEAEDDQLVPFPVAFTHTNASQSLYSTRQHILQTVKRLRRLVFPYMVPLTTVYLF 242
Query: 230 EYLINQGVAPTLLFPID------TTPFV--KYRDIYVTYGTLYQLGVFISRTWGHKLPVK 281
EYLINQ VAPTLLFPI+ + PF KYRDIYVTYGTLYQLGVFISR++GH + ++
Sbjct: 243 EYLINQAVAPTLLFPINGDERSKSMPFFFHKYRDIYVTYGTLYQLGVFISRSFGHLMRMR 302
Query: 282 NLYLFSVLQLINLLITLSQSYYYWTDSISWIMVLIFYEGLIGGSSYVNCFMNILKDVDPN 341
+LY+ + LQ +NL IT+ QS++Y T S +M+LIFYEG +GG+SYVN F+NIL+ DP+
Sbjct: 303 SLYILAFLQGVNLCITVLQSWFYVTHSPWAVMILIFYEGFLGGASYVNTFLNILEQEDPD 362
Query: 342 EREFVLGSVSISDSLGTLIAAFLGIILEPSLCKHQIGTGRPWCRME 387
E EF +G+VSI+DS G +AA LG+ LEP LC+HQI RPWCRME
Sbjct: 363 ETEFAMGAVSIADSFGVFLAALLGLGLEPKLCRHQIADDRPWCRME 408
>SPAC607.09c [R] KOG3880 Predicted small molecule transporter involved in
cellular pH homeostasis (Batten disease protein in
human)
Length = 396
Score = 239 bits (611), Expect = 4e-63
Identities = 152/394 (38%), Positives = 218/394 (54%), Gaps = 10/394 (2%)
Query: 1 MELDRDKKTFAYFWLFGLINNILYVVILSAASDIIGPSLPKSIVLLFDIMPSFLIKLSAP 60
+ L +D K F +FGL+NN+LYV+ILSAA D++G ++ K +VLL +I+PS KLSA
Sbjct: 4 LRLTKDAKVGCCFLIFGLLNNLLYVIILSAALDLVGANVSKGVVLLSNIVPSLACKLSAS 63
Query: 61 FF-VHSIHYDKRXXXXXXXXXXXXXXVS-TRSLWLCLPGIVLASLSSGFGEITFLQLTHF 118
VH + KR ++ + S+ + G+ LA++SS FGEI+FL L+
Sbjct: 64 ILHVHKFKFAKRIGFCVFMSILGMQWIAWSSSVPSKMLGVSLAAISSSFGEISFLHLSSR 123
Query: 119 FGSKSLTGWSSGTGGAGIVGSFSYLLLTTVFRXXXXXXXXXXXXXXXXXXXXY--KINDS 176
+ S SL WSSGTG AG+ G+ SYL++TT F Y + +S
Sbjct: 124 YHSVSLPCWSSGTGLAGLFGASSYLVMTTWFNFSVRSTLIISSFLPLFLLIMYFFVLPES 183
Query: 177 IVSSQVYQSLDMPIDSSDGPIELLKSNGWVDIVKRLSKLVIPY----MIPLSTVYLFEYL 232
+S + PI+S D + N +V+ +K+ + PY M P VY EY
Sbjct: 184 ESTSPSINNNYTPIESIDLRAGHVSFN-FVNSLKQTFIFMQPYLLSHMFPQFLVYFSEYT 242
Query: 233 INQGVAPTLLFPIDTTPFVKYRDIYVTYGTLYQLGVFISRTWGHKLPVKNLYLFSVLQLI 292
IN GVAPTLLFP + F +RD Y TY T+YQ+GVF+SR+ V L ++ Q I
Sbjct: 243 INIGVAPTLLFPPEKAGFSSFRDFYPTYQTVYQIGVFLSRSSISFFTVPYLRTLAITQFI 302
Query: 293 NLLITLSQSYYYWTDSISWIMVLIFYEGLIGGSSYVNCFMNILKDVDPNEREFVLGSVSI 352
LL T+ QS Y T S +++ LIF EGLIGG+ YVN + + L+ + ++RE + +V
Sbjct: 303 ILLFTILQSALYLTSSYHFVLFLIFVEGLIGGTVYVNVY-HSLQTTESSQRELAISTVGS 361
Query: 353 SDSLGTLIAAFLGIILEPSLCKHQIGTGRPWCRM 386
SDS G +A+ + + LEPSLC Q GR WC +
Sbjct: 362 SDSSGIFLASLVSLFLEPSLCHFQADRGRDWCAL 395
>CE09198 [R] KOG3880 Predicted small molecule transporter involved in
cellular pH homeostasis (Batten disease protein in
human)
Length = 424
Score = 191 bits (486), Expect = 1e-48
Identities = 130/405 (32%), Positives = 203/405 (50%), Gaps = 44/405 (10%)
Query: 13 FWLFGLINNILYVVILSAASDIIGP------------------------SLPKSIVLLFD 48
FWL GL NN YV++LSAA DI+ S+ VLL D
Sbjct: 13 FWLLGLCNNYGYVIMLSAAEDILDDQKGTNSSSTNSSTCETHLDSRHCQSISTGAVLLAD 72
Query: 49 IMPSFLIKLSAPFFVHSIHYDKRXXXXXXXXXXXXXXVS-TRSLWLCLPGIVLASLSSGF 107
+P+ +++ + PFF+H + R V+ ++++ + L G+ +ASL G
Sbjct: 73 NLPALVVQTTFPFFMHRFPFGFRAFLVFLLQASSYFVVAYSKNIAMSLAGVCMASLGQGI 132
Query: 108 GEITFLQLTHFFGSKSLTGWSSGTGGAGIVGSFSYLLLTTVFRXXXXXXXXXXXXXXXXX 167
GEITFL + + +++ WSSGTGGAG++GSFSY +T
Sbjct: 133 GEITFLAMAAHYIPETIASWSSGTGGAGLIGSFSYAFITQAGLSPSNTLLVQLFIPVVFA 192
Query: 168 XXXY---KINDSIVSSQVYQSL-DMPIDSSDGPIELLKSNGWVDIVKRLS---------- 213
+ I ++ S ++ S +P + E ++ G V K++
Sbjct: 193 GAYFFLLTIPPTVYSPTIHPSTWIIPKNYDKDVFEAAEARGDVLNTKKVPQRELGPLERI 252
Query: 214 KLVIPY---MIPLSTVYLFEYLINQGVAPTLLFPIDTTPFVKYRDIYVTYGTLYQLGVFI 270
KL+ P M+PL+TVY EY+INQG+ ++F + Y Y LYQLGVFI
Sbjct: 253 KLIGPLLYLMVPLATVYTAEYMINQGLTELIIFNCSQGLSLPLSSQYRWYQVLYQLGVFI 312
Query: 271 SRTWGH--KLPVKNLYLFSVLQLINLLITLSQSYYYWTDSISWIMVLIFYEGLIGGSSYV 328
SR+ ++P+ ++ +LQ +N++ ++ Y++T +I I VLI +EGL+GGS+YV
Sbjct: 313 SRSSVKFFEMPLWLIWCLPILQCVNMIFFFFEAVYWFTPTIIIIFVLIVFEGLLGGSAYV 372
Query: 329 NCFMNILKDVDPNEREFVLGSVSISDSLGTLIAAFLGIILEPSLC 373
N + I K V+P+ RE+ L + S+ +SLGT IAAFL I L +C
Sbjct: 373 NTYNKIHKKVNPDVREYSLSAASMGNSLGTNIAAFLSIPLYNWVC 417
>Hs4502889 [R] KOG3880 Predicted small molecule transporter involved in
cellular pH homeostasis (Batten disease protein in
human)
Length = 438
Score = 186 bits (471), Expect = 7e-47
Identities = 127/399 (31%), Positives = 193/399 (47%), Gaps = 40/399 (10%)
Query: 13 FWLFGLINNILYVVILSAASDII--------------GP--------------SLPKSIV 44
FWL GL NN YVV+LSAA DI+ GP S+ + V
Sbjct: 41 FWLLGLCNNFSYVVMLSAAHDILSHKRTSGNQSHVDPGPTPIPHNSSSRFDCNSVSTAAV 100
Query: 45 LLFDIMPSFLIKLSAPFFVHSIHYDKRXXXXXXXXXXXXXXVS-TRSLWLCLPGIVLASL 103
LL DI+P+ +IKL AP +H + Y R V+ + S+ L G+V AS+
Sbjct: 101 LLADILPTLVIKLLAPLGLHLLPYSPRVLVSGICAAGSFVLVAFSHSVGTSLCGVVFASI 160
Query: 104 SSGFGEITFLQLTHFFGSKSLTGWSSGTGGAGIVGSFSYLLLTTVFRXXXXXXXXXXXXX 163
SSG GE+TFL LT F+ ++ WSSGTGGAG++G+ SYL LT
Sbjct: 161 SSGLGEVTFLSLTAFYPRAVISWWSSGTGGAGLLGALSYLGLTQAGLSPQQTLLSMLGIP 220
Query: 164 XXXXXXXY------KINDSIVSSQVYQSLDMPIDSSDGPIELLKSNGWVDIVKR--LSKL 215
+ + D + + P+ ++ P S+ + + +R + K
Sbjct: 221 ALLLASYFLLLTSPEAQDPGGEEEAESAARQPLIRTEAPESKPGSSSSLSLRERWTVFKG 280
Query: 216 VIPYMIPLSTVYLFEYLINQGVAPTLLFPIDTTPFVKYRDIYVTYGTLYQLGVFISRTWG 275
++ Y++PL VY EY INQG+ L F + + Y Y LYQ GVF SR+
Sbjct: 281 LLWYIVPLVVVYFAEYFINQGLFELLFF---WNTSLSHAQQYRWYQMLYQAGVFASRSSL 337
Query: 276 HKLPVKNLYLFSVLQLINLLITLSQSYYYWTDSISWIMVLIFYEGLIGGSSYVNCFMNIL 335
++ + ++LQ +NL+ L+ ++ + SI + ++I YEGL+GG++YVN F NI
Sbjct: 338 RCCRIRFTWALALLQCLNLVFLLADVWFGFLPSIYLVFLIILYEGLLGGAAYVNTFHNIA 397
Query: 336 KDVDPNEREFVLGSVSISDSLGTLIAAFLGIILEPSLCK 374
+ REF + + ISD+LG ++ L + L LC+
Sbjct: 398 LETSDEHREFAMAATCISDTLGISLSGLLALPLHDFLCQ 436
>CE07802 [R] KOG3880 Predicted small molecule transporter involved in
cellular pH homeostasis (Batten disease protein in
human)
Length = 435
Score = 177 bits (449), Expect = 2e-44
Identities = 131/422 (31%), Positives = 196/422 (46%), Gaps = 53/422 (12%)
Query: 5 RDKKTFAYFWLFGLINNILYVVILSAASDIIGPS-------------------LPKSIVL 45
R + FWL GL NN YVV+LSAA DI+ + VL
Sbjct: 10 RPPRNSVAFWLLGLCNNFAYVVMLSAAKDILEKDAKHIEKPCREHVTTRECQMMSTGSVL 69
Query: 46 LFDIMPSFLIKLSAPFFVHSIHYDKRXXXXXXXXXXXXXXVS-TRSLWLCLPGIVLASLS 104
L DI+P+ LIK++AP F+H + + R V + S L L G+VLAS
Sbjct: 70 LADIIPALLIKITAPMFIHRVPFGIRHSIVVLLQASSFLIVGISDSTALALFGVVLASFG 129
Query: 105 SGFGEITFLQLTHFFGSKSLTGWSSGTGGAGIVGSFSYLLLTTVF------RXXXXXXXX 158
SG GEI++L L+ + S + WSSGTGGAG++G+ +Y LLT +
Sbjct: 130 SGLGEISYLALSSNYPSTVVASWSSGTGGAGLIGASAYALLTDSKLLAISPKHTMFIMLT 189
Query: 159 XXXXXXXXXXXXYKINDSI------------VSSQVYQSLDM---PIDSSDGPIELLKS- 202
KI S+ V+ + DM ++ +G + + +
Sbjct: 190 LPALFSVSYWSILKIPHSVQRAHFLQPSTWLVTGDFIPADDMRREEVEEEEGLLGIREDR 249
Query: 203 NGWVDIVKRLSKL---------VIPYMIPLSTVYLFEYLINQGVAPTLLFPIDTTPFVKY 253
VD+ R + ++ +MIPL +VYL EY INQG+ L F +
Sbjct: 250 ENSVDVTSRRRQTSTTLERVLPLLKFMIPLISVYLAEYYINQGLLELLEFDCSHGFSMSS 309
Query: 254 RDIYVTYGTLYQLGVFISRTWGHKLPVKNLYL--FSVLQLINLLITLSQSYYYWTDSISW 311
Y + YQLGVFISR+ + + + YL +VLQ+ N + Y + I
Sbjct: 310 ESQYRWFQVTYQLGVFISRSSSNYVTIPTQYLKSLAVLQIFNAGFFTITAIYSFLPHILI 369
Query: 312 IMVLIFYEGLIGGSSYVNCFMNILKDVDPNEREFVLGSVSISDSLGTLIAAFLGIILEPS 371
++I +EGL+GG+SYVN F + K++ + REF +G VSISD++G + A FL + +
Sbjct: 370 AFLVILFEGLLGGASYVNTFRAVHKEIPADSREFSMGVVSISDTIGIVFAGFLAMPVHNR 429
Query: 372 LC 373
+C
Sbjct: 430 IC 431
>CE27645 [R] KOG3880 Predicted small molecule transporter involved in
cellular pH homeostasis (Batten disease protein in
human)
Length = 417
Score = 176 bits (446), Expect = 5e-44
Identities = 136/405 (33%), Positives = 192/405 (46%), Gaps = 53/405 (13%)
Query: 13 FWLFGLINNILYVVILSAASDIIG---------------PSL-------PKSIVLLFDIM 50
F+L GL NN YV++LSAA DI+ P++ P + VLL D +
Sbjct: 16 FFLIGLCNNYGYVIMLSAAEDILSEQHGKVKSNATDLCLPAITKRECKPPVAEVLLSDNL 75
Query: 51 PSFLIKLSAPFFVHSIHYDKRXXXXXXXXXXXXXXVS-TRSLWLCLPGIVLASLSSGFGE 109
PS ++KL+ PFF+ + R V+ + S+ + L G+V SL G GE
Sbjct: 76 PSLIVKLTFPFFMDRFPFGFRVAIVCLLQATSYFVVAFSVSIPMSLAGVVFVSLGGGLGE 135
Query: 110 ITFLQLTHFFGSKSLTGWSSGTGGAGIVGSFSYLLLTT--VFRXXXXXXXXXXXXXXXXX 167
ITFL L+ + ++ GWSSGTG AG++GSFSY LT +
Sbjct: 136 ITFLGLSAHYQRIAIAGWSSGTGMAGLLGSFSYAFLTEPHMANLTPKVALLIQLFIPVLF 195
Query: 168 XXXYKINDSIVSSQVYQSLDMPIDSSDGPIELLKSNGWVDIV--------------KRLS 213
Y I S +LD P + G+ D + RL
Sbjct: 196 AFAYFILLKKPESVYSPTLD--------PKSWIVPKGYDDFIVSEHRVPQRELGPSDRL- 246
Query: 214 KLVIPY---MIPLSTVYLFEYLINQGVAPTLLFPIDTTPFVKYRDIYVTYGTLYQLGVFI 270
KL++P MIPL+ VY+ EY+INQG+ ++F + Y Y LYQ GVF+
Sbjct: 247 KLILPMLHLMIPLAIVYVGEYMINQGMTQQIVFDCAHGFNLSLHSQYRWYQVLYQFGVFV 306
Query: 271 SRTWGH--KLPVKNLYLFSVLQLINLLITLSQSYYYWTDSISWIMVLIFYEGLIGGSSYV 328
SR+ +LP+ LYL LQL N+L + Y++ I+ I LI +EGL GGSSYV
Sbjct: 307 SRSSIRLVELPMWMLYLLPFLQLTNMLFFFFDALYWFVPQIAIIFALIIFEGLFGGSSYV 366
Query: 329 NCFMNILKDVDPNEREFVLGSVSISDSLGTLIAAFLGIILEPSLC 373
N F I V+P+ RE+ L + S+ DS+G AA + I L +C
Sbjct: 367 NTFHKIHNKVEPDVREYCLSAASMGDSIGVNFAAGVSIPLHYWMC 411
>7293939 [R] KOG3880 Predicted small molecule transporter involved in
cellular pH homeostasis (Batten disease protein in
human)
Length = 407
Score = 163 bits (413), Expect = 4e-40
Identities = 110/369 (29%), Positives = 180/369 (47%), Gaps = 21/369 (5%)
Query: 13 FWLFGLINNILYVVILSAASDIIGPS---LPKSIVLLFDIMPSFLIKLSAPFFVHSIHYD 69
+W+ GL NN YV S S G + + +LL D++PS +K+ PFF +++
Sbjct: 41 YWILGLCNNYGYVNDESEESSS-GRNCHLVSTGAILLADVLPSLFVKILMPFFPFWVNF- 98
Query: 70 KRXXXXXXXXXXXXXXVS-TRSLWLCLPGIVLASLSSGFGEITFLQLTHFFGSKSLTGWS 128
R V + W+ L G+++ S SSG GE TFL + + ++ WS
Sbjct: 99 -RIALAVAFSAAGFLLVGFANAEWMALLGVIITSASSGIGETTFLAYSSRYNKNVISTWS 157
Query: 129 SGTGGAGIVGSFSYLLLTTVFRXXXXXXXXXXXXXXXXXXXXYKI----NDSIVSSQVYQ 184
SGTGGAG++GS SY L ++ + + I+ +
Sbjct: 158 SGTGGAGVIGSLSYASLRSLDFSPRDTMLIMLIFPAIEAFAFWLLLRRPQVDILPVTTVE 217
Query: 185 SLDMPIDSSDGPIELLKSNGWVDIVKRLSKLVIPYMIPLSTVYLFEYLINQGVAPTLLFP 244
S ++ I S + P+ K + +K L K YM+PL VY FEY INQG+ + F
Sbjct: 218 STEVLI-SDEKPLVGFKEK--LFYIKHLFK----YMLPLCLVYFFEYFINQGLFELVYFE 270
Query: 245 IDTTPFVKYRDIYVTYGTLYQLGVFISRTWGHKLPVKNLYLFSVLQLINLLITLSQSYYY 304
F+ Y YQ+GVFISR+ + + ++L S+ Q +N++ L++ ++
Sbjct: 271 ---DIFLDKDSQYRWLNVDYQIGVFISRSSVNLFQLDKIWLMSIFQFVNVVYFLTEVIWW 327
Query: 305 WTDSISWIMVLIFYEGLIGGSSYVNCFMNILKDVDPNEREFVLGSVSISDSLGTLIAAFL 364
+T SI + ++ +EGL+GG +YVN F + K++ P ++F + V SDS G +A FL
Sbjct: 328 YTPSIWIVFAIVLWEGLLGGGAYVNTFYRMSKEISPERQQFAMAMVVQSDSYGIALAGFL 387
Query: 365 GIILEPSLC 373
I + ++C
Sbjct: 388 AIPVHNAIC 396
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.326 0.143 0.439
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,770,977
Number of Sequences: 60738
Number of extensions: 811770
Number of successful extensions: 2481
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2446
Number of HSP's gapped (non-prelim): 12
length of query: 387
length of database: 30,389,216
effective HSP length: 108
effective length of query: 279
effective length of database: 23,829,512
effective search space: 6648433848
effective search space used: 6648433848
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)