ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI0486 good Q KOG1210 Secondary metabolites biosynthesis, transport and catabolism Predicted 3-ketosphinganine reductase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI0486 164762 163824 -313
(313 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YBR265w [Q] KOG1210 Predicted 3-ketosphinganine reductase 262 4e-70
SPCC1450.15_1 [Q] KOG1210 Predicted 3-ketosphinganine reductase 107 2e-23
ECU11g1070 [Q] KOG1210 Predicted 3-ketosphinganine reductase 75 9e-14
CE26060 [Q] KOG1210 Predicted 3-ketosphinganine reductase 69 7e-12
Hs4503817 [Q] KOG1210 Predicted 3-ketosphinganine reductase 62 8e-10
At3g06060 [Q] KOG1210 Predicted 3-ketosphinganine reductase 59 7e-09
7301303 [Q] KOG1210 Predicted 3-ketosphinganine reductase 59 1e-08
At5g19200 [Q] KOG1210 Predicted 3-ketosphinganine reductase 57 3e-08
>YBR265w [Q] KOG1210 Predicted 3-ketosphinganine reductase
Length = 320
Score = 262 bits (670), Expect = 4e-70
Identities = 143/323 (44%), Positives = 198/323 (61%), Gaps = 21/323 (6%)
Query: 4 FNCNGQVILISGGSQGLGESFAKRFVQDDDGPGSNTNKVIIVSRSQSKLVKACERIGVDG 63
F QV+LI+GGSQGLG+ FAK++ + + K+IIVSRS+++L+ C I ++
Sbjct: 3 FTLEDQVVLITGGSQGLGKEFAKKYYNE-----AENTKIIIVSRSEARLLDTCNEIRIEA 57
Query: 64 VSLDRYVNDTNRN----------ETKLIYHSCDTSSYDKVALMFKLLVKSELVPSQVYMC 113
L R D + E +L Y+ CD S Y+ V +F L +L+P+Q C
Sbjct: 58 -HLRRETTDEGQVQHKLAAPLDLEQRLFYYPCDLSCYESVECLFNALRDLDLLPTQTLCC 116
Query: 114 AGGSIPKLFLDLTPEELQNGITTNYSTAVNLAH-VSLKHDVP--HLLFFSSEVAFFPFIG 170
AGG++PKLF L+ EL G+ NY T +N+AH ++L HL+ FSS A +PF+G
Sbjct: 117 AGGAVPKLFRGLSGHELNLGMDINYKTTLNVAHQIALAEQTKEHHLIIFSSATALYPFVG 176
Query: 171 YAQYAPLKQSIRSLVAILRQEHSSTRITCVYPGNFQSEGFDLENITKPAITKEIEGPSNP 230
Y+QYAP K +I+SLVAILRQE ++ RI+CVYPGNF+SEGF +E +TKP ITK IEGPS+
Sbjct: 177 YSQYAPAKAAIKSLVAILRQELTNFRISCVYPGNFESEGFTVEQLTKPEITKLIEGPSDA 236
Query: 231 VTAAQCRDKIISSLKWGLDDITTDSIGWLLMACDQGLNKHSTSQFMFVFSWILGALLNIT 290
+ Q D I SL G +D+ TD +GW++M D GL S+F+ WI G L NI
Sbjct: 237 IPCKQACDIIAKSLARGDEDVFTDFVGWMIMGMDLGLTA-KKSRFV-PLQWIFGVLSNIL 294
Query: 291 IVPIYMLICKFQIYQWKRNKDTK 313
+VP YM+ C + I +W R D K
Sbjct: 295 VVPFYMVGCSWYIRKWFRENDGK 317
>SPCC1450.15_1 [Q] KOG1210 Predicted 3-ketosphinganine reductase
Length = 373
Score = 107 bits (267), Expect = 2e-23
Identities = 84/309 (27%), Positives = 140/309 (45%), Gaps = 34/309 (11%)
Query: 11 ILISGGSQGLGESFAKRFVQDDDGPGSNTNKVIIVSRSQSKLVKACERIGVDGVSLDRYV 70
IL++GGSQGLG++ AK V G+N V IV+R+ +KL +A + + D+ V
Sbjct: 25 ILVTGGSQGLGKAIAKELVLR----GAN---VTIVARTVTKLQEAVAELSDSKIHEDQQV 77
Query: 71 NDTNRNETKLIYHSCDTSSYDKVALMFKLLVKSELVPSQVYMCAGGSIPKLFLDLTPEEL 130
+ + S D +SY+ V M + L P V CAG IP F +L P
Sbjct: 78 S----------FESVDLTSYESVHSMIERL---PFCPDHVVHCAGSCIPGFFTELDPSVF 124
Query: 131 QNGITTNYSTAVNLAHVSLKHDVP-------HLLFFSSEVAFFPFIGYAQYAPLKQSIRS 183
+ + NY +V + H +++ +L S ++ P IGY+ Y+P+K ++R+
Sbjct: 125 EKQMRQNYLASVYVCHAAIRRMKEISPSYSRRILLVGSLLSSLPIIGYSAYSPVKAAVRN 184
Query: 184 LVAILRQE--HSSTRITCVYPGNFQSEGFDLENITKPAITKEIEGPSNPVTAAQCRDKII 241
L LRQE ++ P S G++ EN KP + ++EG + T + +
Sbjct: 185 LADSLRQECILYDIEVSVYLPSTILSPGYEQENTLKPELVLQMEGMDSVQTCEEAASHCM 244
Query: 242 SSLKWGLDDITTDSIGWLLMACDQGLNKHSTSQFMFVFSWILGALLNITIVPIYMLICKF 301
+ L G I +S G L+ + + H ++F AL+++ P Y
Sbjct: 245 TGLDRGDFLIANESTGHLMKNHCRNSSPHDNPILEYLF-----ALVSLLAWPFYRRKLDS 299
Query: 302 QIYQWKRNK 310
+YQ+ K
Sbjct: 300 LVYQYALEK 308
>ECU11g1070 [Q] KOG1210 Predicted 3-ketosphinganine reductase
Length = 299
Score = 75.5 bits (184), Expect = 9e-14
Identities = 66/247 (26%), Positives = 103/247 (40%), Gaps = 39/247 (15%)
Query: 3 GFNCNGQVILISGGSQGLGESFAKRFVQDDDGPGSNTNKVIIVSRSQSKLVKACERIGVD 62
G + G+ ILI GGS GLG +FA+ + + G N V + SRS KL R G+
Sbjct: 25 GISIRGRKILIIGGSSGLGLAFAR--ILKEAG-----NDVTVTSRSMKKLEALQRRWGLK 77
Query: 63 GVSLDRYVNDTNRNETKLIYHSCDTSSYDKVALMFKLLVKSELVPSQVYMCAGGSIPKLF 122
LD D + + +D ++ CAG S P F
Sbjct: 78 TQVLDVNAADVP-----------EPTDFD-----------------YIFCCAGVSYPSYF 109
Query: 123 LDLTPEELQNGITTNY-STAVNLAHVSLKHDVP-HLLFFSSEVAFFPFIGYAQYAPLKQS 180
+D E + TNY T L H + + P + S +A F G++ Y+P K +
Sbjct: 110 IDQPLEVFEETANTNYLGTVAMLKHYAAVNKKPVKFIMIGSTLALLTFPGFSSYSPSKTA 169
Query: 181 IRSLVAILRQEHSSTRITCVY--PGNFQSEGFDLENITKPAITKEIEGPSNPVTAAQCRD 238
+ SL +R E + + + GF+ EN KPA T+ +EG +P +A +
Sbjct: 170 LLSLFYTVRDEMGKIGVELYLYNTASILTPGFERENRAKPAYTRAVEGMVSPSSAEKRAT 229
Query: 239 KIISSLK 245
++ +K
Sbjct: 230 AFLNGMK 236
>CE26060 [Q] KOG1210 Predicted 3-ketosphinganine reductase
Length = 347
Score = 69.3 bits (168), Expect = 7e-12
Identities = 65/257 (25%), Positives = 106/257 (40%), Gaps = 24/257 (9%)
Query: 12 LISGGSQGLGESFAKRFVQDDDGPGSNTNKVIIVSRSQSKLVKACERIGVDGVSLDRYVN 71
+++GGS+G+G A ++ V IV+R+ L KAC + V +
Sbjct: 42 IVTGGSKGIGFQLAVGLIE-------RGCHVTIVARNVKDLEKACADLQV--------LA 86
Query: 72 DTNRNETKLIYHSCD-TSSYDKVALMFKLLVKSELVPSQVYMC-AGGSIPKLFLDLTPEE 129
D K+ + S D T YD + F + EL P + + AG S+ F +L +
Sbjct: 87 DQRGQRQKVHWKSIDMTGGYDVIKSAFDDAAR-ELGPIDILINNAGHSVQAPFCELPITD 145
Query: 130 LQNGITTNYSTAVNLAHVSLK----HDVPHLLFFSSEVAFFPFIGYAQYAPLKQSIRSLV 185
+ + NY +AV+ + H+ F SS F GY+ Y+P K ++R
Sbjct: 146 FEKQMAVNYLSAVHATRAVVDDMKTRKTGHISFVSSAAGQFAIFGYSAYSPTKFALRGFA 205
Query: 186 AILRQE--HSSTRITCVYPGNFQSEGFDLENITKPAITKEIEGPSNPVTAAQCRDKIISS 243
L E + +YP N +EGF +E T P TK++ + T + +
Sbjct: 206 DTLHMELLPYKVNVGVLYPPNTDTEGFKVELETMPEETKKMSEAAGLFTPKDVAEAHLKD 265
Query: 244 LKWGLDDITTDSIGWLL 260
+ G T GW+L
Sbjct: 266 IADGNYATTMGLDGWML 282
>Hs4503817 [Q] KOG1210 Predicted 3-ketosphinganine reductase
Length = 332
Score = 62.4 bits (150), Expect = 8e-10
Identities = 63/267 (23%), Positives = 118/267 (43%), Gaps = 24/267 (8%)
Query: 8 GQVILISGGSQGLGESFAKRFVQDDDGPGSNTNKVIIVSRSQSKLVKACERIGVDGVSLD 67
G ++++GGS G+G+ A + + +V+R++ KL++A + I + ++ D
Sbjct: 32 GAHVVVTGGSSGIGKCIAIECYKQGAF-------ITLVARNEDKLLQAKKEIEMHSIN-D 83
Query: 68 RYVNDTNRNETKLIYHSCDTSS-YDKVALMFKLLVKSELVPSQVYMCAGGSIPKLFLDLT 126
+ V ++ S D S Y++V + K + + CAG ++ F DL
Sbjct: 84 KQV---------VLCISVDVSQDYNQVENVIKQAQEKLGPVDMLVNCAGMAVSGKFEDLE 134
Query: 127 PEELQNGITTNYSTAVNLAHVSL----KHDVPHLLFFSSEVAFFPFIGYAQYAPLKQSIR 182
+ ++ NY +V + + + V ++F SS+ G+ Y+ K +IR
Sbjct: 135 VSTFERLMSINYLGSVYPSRAVITTMKERRVGRIVFVSSQAGQLGLFGFTAYSASKFAIR 194
Query: 183 SLVAILRQEHS--STRITCVYPGNFQSEGFDLENITKPAITKEIEGPSNPVTAAQCRDKI 240
L L+ E + IT YP + + GF EN TKP T+ I ++ Q +I
Sbjct: 195 GLAEALQMEVKPYNVYITVAYPPDTDTPGFAEENRTKPLETRLISETTSVCKPEQVAKQI 254
Query: 241 ISSLKWGLDDITTDSIGWLLMACDQGL 267
+ G + + S G++L A G+
Sbjct: 255 VKDAIQGNFNSSLGSDGYMLSALTCGM 281
>At3g06060 [Q] KOG1210 Predicted 3-ketosphinganine reductase
Length = 327
Score = 59.3 bits (142), Expect = 7e-09
Identities = 76/315 (24%), Positives = 127/315 (40%), Gaps = 51/315 (16%)
Query: 13 ISGGSQGLGESFAKRFVQDDDGPGSNTNKVIIVSRSQSKLVKACERIGV-DGVSLDRYVN 71
I+GGS G+G + A R S +V I++RS SKL +A + I + GV + +
Sbjct: 44 ITGGSSGIGLALAHR-------AASEGARVSILARSGSKLEEAKKSIQLATGVEVATF-- 94
Query: 72 DTNRNETKLIYHSCDTSSYDKVALMFKLLVKSELVPSQVYMCAGGSIP-KLFLDLTPEEL 130
S D YD V+ E P V + G K + +PE++
Sbjct: 95 ------------SADVRDYDAVSKAI-----DESGPIDVLIVNQGVFTAKELVKHSPEDV 137
Query: 131 QNGITTNYSTAVNLAHVSLK-------HDVPHLLFFSSEVAFFPFIGYAQYAPLKQSIRS 183
+ I N + N+ +L + SS+ GYA Y+ K ++
Sbjct: 138 KFTIDVNLVGSFNVIKAALPAMKARKDRGPASISLVSSQAGQVGVYGYAAYSASKFGLQG 197
Query: 184 LVAILRQEHSST--RITCVYPGNFQSEGFDLENITKPAITKEIEGPSNPVTAAQCRDKII 241
L L+QE S +T ++P + + GF+ E ++P +T I S + + K +
Sbjct: 198 LAQALQQEVISDDIHVTLIFPPDTNTPGFEEEQKSRPEVTAIIAASSGSMETEEVAKKAM 257
Query: 242 SSLKWGLDDITTDSIGWLLMACDQGLNKHSTSQFMFVFSWILGALLNITIVPIYMLICKF 301
+K G ++ + G+LL G++ S+ L L IT PI ++ F
Sbjct: 258 DGIKAGNFTVSCNFEGFLLSLATTGMSPQR--------SFWLAFLEVITAGPIRLIALFF 309
Query: 302 Q------IYQWKRNK 310
Q I +W + K
Sbjct: 310 QWDWYKAIEKWSKTK 324
>7301303 [Q] KOG1210 Predicted 3-ketosphinganine reductase
Length = 336
Score = 58.5 bits (140), Expect = 1e-08
Identities = 65/264 (24%), Positives = 116/264 (43%), Gaps = 32/264 (12%)
Query: 8 GQVILISGGSQGLGESFAKRFVQDDDGPGSNTNKVIIVSRSQSKLVKACERIGVDGVSLD 67
G+ ++++GGS+G+G A G+N V +++R + L A V+L
Sbjct: 35 GRHVVVTGGSKGIGLCLAVECAMK----GAN---VTVIARDEKMLSGA--------VALM 79
Query: 68 RYVNDTNRNETKLIYHSCDTSS-YDKVALMFKLLVKSELVPSQVYM---CAGGSIPKLFL 123
+ R + K Y S D S YD+VA K+L + E +Y CAG +I +F
Sbjct: 80 EVIRQ--RPDQKFQYRSLDISGDYDQVA---KVLGEIEDSFGPIYTLINCAGMAICGVFE 134
Query: 124 DLTPEELQNGITTNYSTAVNLAHVSL----KHDVPHLLFFSSEVAFFPFIGYAQYAPLKQ 179
+++ +++ + N+ N L K ++ +S+ A F GY Y+ K
Sbjct: 135 EVSVQDVHKLMNVNFFGTYNCTRYVLPKMKKAGDGIIVITASQAAMFGIYGYGPYSATKY 194
Query: 180 SIRSL---VAILRQEHSSTRITCVYPGNFQSEGFDLENITKPAITKEIEGPSNPVTAAQC 236
++R++ +A+ +EH + +T P + + GF+ E +KP TK I G +
Sbjct: 195 ALRAMAETIAMESREHGVS-VTLAMPCDTNTPGFEEEEKSKPRETKIISGGGGLIEPEVM 253
Query: 237 RDKIISSLKWGLDDITTDSIGWLL 260
I+ G T + WL+
Sbjct: 254 AKAILKDALKGKFTSTVGAESWLI 277
>At5g19200 [Q] KOG1210 Predicted 3-ketosphinganine reductase
Length = 331
Score = 57.4 bits (137), Expect = 3e-08
Identities = 72/308 (23%), Positives = 122/308 (39%), Gaps = 48/308 (15%)
Query: 11 ILISGGSQGLGESFAKRFVQDDDGPGSNTNKVIIVSRSQSKLVKACERIGV-DGVSLDRY 69
+ I+GGS G+G + A R V S KV I++RS KL +A I + GV + +
Sbjct: 40 VFITGGSSGIGLALAHRAV-------SEGAKVSILARSTEKLAEAKRSIQLATGVEVATF 92
Query: 70 VNDTNRNETKLIYHSCDTSSYDKVALMFKLLVKSELVPSQVYMCAGGS-IPKLFLDLTPE 128
S D YD V+ E P V + G I K +PE
Sbjct: 93 --------------SADVRDYDAVSKAI-----DESGPIDVLIVNQGVFIGKELEKQSPE 133
Query: 129 ELQNGITTNYSTAVNLAHVSLK-------HDVPHLLFFSSEVAFFPFIGYAQYAPLKQSI 181
E++ I N + + N+ +L + SS+ GY Y+ K +
Sbjct: 134 EVKFMIDVNLTGSFNVIKAALPAMKAREGRGPASISLVSSQAGQAGIYGYTAYSASKFGL 193
Query: 182 RSLVAILRQEHSST--RITCVYPGNFQSEGFDLENITKPAITKEIEGPSNPVTAAQCRDK 239
+ L L+QE S +T ++P + + GF+ E +P +T I S + +
Sbjct: 194 QGLAQALQQEVISDGIHVTLLFPPDTDTPGFEQELKKRPELTSIIAASSGSMKTNEVAKI 253
Query: 240 IISSLKWGLDDITTDSIGWLLMACDQGLNKHSTSQFMFVFSWILGALLNITIVPIYMLIC 299
+K G +T IG+LL G++ S ++ + + G L+ + +
Sbjct: 254 CFDGIKAGKFTVTCHFIGFLLSIASTGMSPQG-SFWLALTEVMFGGLIRLASL------- 305
Query: 300 KFQIYQWK 307
++QW+
Sbjct: 306 ---VFQWQ 310
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.137 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,769,977
Number of Sequences: 60738
Number of extensions: 755552
Number of successful extensions: 1970
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 1953
Number of HSP's gapped (non-prelim): 9
length of query: 313
length of database: 30,389,216
effective HSP length: 106
effective length of query: 207
effective length of database: 23,950,988
effective search space: 4957854516
effective search space used: 4957854516
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)