ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactVI0520   suspect: LH   K     KOG2478  Transcription                                                  Putative RNA polymerase II regulator

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactVI0520  175031  176314 428  
         (428 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YBR279w [K] KOG2478 Putative RNA polymerase II regulator              305   9e-83
SPAC664.03 [K] KOG2478 Putative RNA polymerase II regulator            72   1e-12

>YBR279w [K] KOG2478 Putative RNA polymerase II regulator
          Length = 445

 Score =  305 bits (781), Expect = 9e-83
 Identities = 172/428 (40%), Positives = 248/428 (57%), Gaps = 25/428 (5%)

Query: 3   RQELIAKIRYENEXXXXXXXXXXIKYQASPDEDVXXXXXXXXXXXXXNVTPLIDLDHDLG 62
           +QE IA I+Y+N           + Y  SP+ +              NVT LI  D DLG
Sbjct: 4   KQEYIAPIKYQNSLPVPQLPPKLLVYPESPETNADSSQLINSLYIKTNVTNLIQQDEDLG 63

Query: 63  MPLDLLTIPGVLNQQNVGGLFGYDNIKLKQEDRVLLRDPGMDKVVQSDLSKVTFLRRTEY 122
           MP+DL+  PG+LN+ +   L+G+DN+KL ++DR+LLRDP +D++ ++D+SKVTFLRRTEY
Sbjct: 64  MPVDLMKFPGLLNKLDSKLLYGFDNVKLDKDDRILLRDPRIDRLTKTDISKVTFLRRTEY 123

Query: 123 VIST----NMSKADKVKRTRSALQNEETLTPEQVLEKVESTFNHRTDDLEKLRHPIKKKV 178
           V +T    + +   + +R      ++E L    ++ +VE TFN +TD   K +HP+KK V
Sbjct: 124 VSNTIAAHDNTSLKRKRRLDDGDSDDENLDVNHIISRVEGTFN-KTD---KWQHPVKKGV 179

Query: 179 KAVNSWNLLPDTVSMDQGYFTVKFVGSAALDGKEKEKYDMSTALLRPVELEEDEWISAYX 238
           K V  W+LLPDT SMDQ YF +KF+GSA+LD KEK+   ++T + RPVELEEDEWIS Y 
Sbjct: 180 KMVKKWDLLPDTASMDQVYFILKFMGSASLDTKEKK--SLNTGIFRPVELEEDEWISMYA 237

Query: 239 XXXXXXXXXXXXXXQQIDEI---SHDDSIYKFKRFRDFDMKQIEPETQSSDLALRYDNDK 295
                         + +DE+   SH+  IYKFKR RD+DMKQ+  E   ++LA+R ++  
Sbjct: 238 TDHKDSAILENELEKGMDEMDDDSHEGKIYKFKRIRDYDMKQV-AEKPMTELAIRLNDKD 296

Query: 296 RTVYYKPXXXXXXXXXXXVNDVIRPLVREHNWDQINVRLRAPTTKETKVRDAIRMRYDPI 355
              YYKP           VND+I+PLV+EH+ DQ+NV LR P+TKE  +RD +RM++DPI
Sbjct: 297 GIAYYKPLRSKIELRRRRVNDIIKPLVKEHDIDQLNVTLRNPSTKEANIRDKLRMKFDPI 356

Query: 356 DFPALXXXXXXXXXXXXXXXXSQNGED--SQNQGLASTENVKPTASDEVKEESVQSATNE 413
           +F  +                 +  ED   +++G + TE  +    +E  EE  Q   NE
Sbjct: 357 NFATV---------DEEDDEDEEQPEDVKKESEGDSKTEGSEQEGENEKDEEIKQEKENE 407

Query: 414 LASQKDED 421
              +  +D
Sbjct: 408 QDEENKQD 415


>SPAC664.03 [K] KOG2478 Putative RNA polymerase II regulator
          Length = 457

 Score = 72.4 bits (176), Expect = 1e-12
 Identities = 71/261 (27%), Positives = 103/261 (39%), Gaps = 26/261 (9%)

Query: 55  IDLDHDLGMPLDLLTIPGVLNQQNVGGLFGYDNIKLKQEDRVLLRDPGMDKVVQSDLSKV 114
           I+ D +LGMPLDL  I G              ++ L   DR LL+  G      S   +V
Sbjct: 55  IENDIELGMPLDLAGITGFFEGDTSWMHSDLSSVNLDPIDRSLLKVAGGSG---STHLEV 111

Query: 115 TFLRRTEYVIS------TNMSKADKVKRTRSALQNEETLTPEQV---LEKVESTFNHRTD 165
            FLRRTEY+ S      +N         T  AL  +   +  +V   LE +  +F+    
Sbjct: 112 PFLRRTEYISSEVARAASNRGNLRLTASTSKALAEQRGRSLREVPKQLEAINESFDVVQQ 171

Query: 166 DLEKLRHPIKKKVKAVNSWNLLPDT-----------VSMDQGYFTVKFVGSAALDGKEKE 214
            LE+L+HP K  +K V++WNLLP+T           V+ D    +  +     L      
Sbjct: 172 PLEQLKHPTKPDLKPVSAWNLLPNTSMAGIQHLMLRVADDLSERSHSYSSLVNLQEGHNL 231

Query: 215 KYDMSTALLRPVELEEDEWISAYXXXXXXXXXXXXXXXQQIDEISHDDSIYKFKRFRDFD 274
                 AL  P   E +E++S Y                   ++ H+  +Y    FR+FD
Sbjct: 232 TKRHEVALFMPSSAEGEEFLSYYLPSEETAEEIQAKVNDASADV-HEPFVY--NHFRNFD 288

Query: 275 MKQIEPETQSSDLALRYDNDK 295
                  T   DL L +  DK
Sbjct: 289 ASMHVNSTGLEDLCLTFHTDK 309


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.312    0.131    0.363 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,520,288
Number of Sequences: 60738
Number of extensions: 796845
Number of successful extensions: 1986
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1977
Number of HSP's gapped (non-prelim): 2
length of query: 428
length of database: 30,389,216
effective HSP length: 109
effective length of query: 319
effective length of database: 23,768,774
effective search space: 7582238906
effective search space used: 7582238906
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)