ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI0523 good R KOG3101 General function prediction only Esterase D
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI0523 176686 177570 295
(295 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJL068c [R] KOG3101 Esterase D 413 e-115
At2g41530 [R] KOG3101 Esterase D 227 2e-59
CE25312 [R] KOG3101 Esterase D 217 2e-56
Hs20547663 [R] KOG3101 Esterase D 216 4e-56
7300589 [R] KOG3101 Esterase D 192 6e-49
>YJL068c [R] KOG3101 Esterase D
Length = 299
Score = 413 bits (1061), Expect = e-115
Identities = 192/300 (64%), Positives = 235/300 (78%), Gaps = 8/300 (2%)
Query: 3 LKINSAIAVCGGKLLKLSHFSSCTGTDMDVNLYLPKQYYTS-IDDSKLIPSVLYLSGLTC 61
+K+ +VCGG+L+KLSH S+ T T M+VN+YLPK YY +K IP+V YLSGLTC
Sbjct: 1 MKVVKEFSVCGGRLIKLSHNSNSTKTSMNVNIYLPKHYYAQDFPRNKRIPTVFYLSGLTC 60
Query: 62 TPQNASEKAFWQIQADKFGFAVIFPDTSPRGDGVATDPEGSWDFGLGAGFYVNATEQPYS 121
TP NASEKAFWQ QADK+GFA++FPDTSPRGD VA DPEGSWDFG GAGFY+NAT++PY+
Sbjct: 61 TPDNASEKAFWQFQADKYGFAIVFPDTSPRGDEVANDPEGSWDFGQGAGFYLNATQEPYA 120
Query: 122 SHYKMYDYVHKELPEVLAEEFGS----KVDFFKNISITGHSMGGFGALSGYLKNIN--RY 175
HY+MYDY+HKELP+ L F K+DF N++ITGHSMGG+GA+ GYLK + RY
Sbjct: 121 QHYQMYDYIHKELPQTLDSHFNKNGDVKLDFLDNVAITGHSMGGYGAICGYLKGYSGKRY 180
Query: 176 KSCSAFAPIVNPSAVPWGQKAFTGYLGSSNKEAWDGYDPTKLIKHVENLNGNKILIHVGS 235
KSCSAFAPIVNPS VPWGQKAF GYLG K W+ YDP LIK++ ++ ++ILIHVG
Sbjct: 181 KSCSAFAPIVNPSNVPWGQKAFKGYLG-EEKAQWEAYDPCLLIKNIRHVGDDRILIHVGD 239
Query: 236 NDPFLEKHLRPHLLQEAAKGTFWEGKIEVNFEDGFDHSYYFISTFVPQHAEFHAKHLGLI 295
+DPFLE+HL+P LL EA K T W+ +E+ GFDHSYYF+STFVP+HAEFHA++LGLI
Sbjct: 240 SDPFLEEHLKPELLLEAVKATSWQDYVEIKKVHGFDHSYYFVSTFVPEHAEFHARNLGLI 299
>At2g41530 [R] KOG3101 Esterase D
Length = 284
Score = 227 bits (578), Expect = 2e-59
Identities = 131/277 (47%), Positives = 171/277 (61%), Gaps = 12/277 (4%)
Query: 18 KLSHFSSCTGTDMDVNLYLPKQYYTSIDDSKLIPSVLYLSGLTCTPQNASEKAFWQIQAD 77
+ HFS G M ++Y P S S P + +LSGLTCT +N K+ Q A
Sbjct: 20 RYKHFSETLGCSMTFSIYFPP----SASSSHKSPVLYWLSGLTCTDENFIIKSGAQRAAS 75
Query: 78 KFGFAVIFPDTSPRGDGVATDPEGSWDFGLGAGFYVNATEQPYSSHYKMYDYVHKELPEV 137
G A++ PDTSPRG V + + S+DFG+GAGFY+NAT++ + + ++MYDYV KELP++
Sbjct: 76 THGIALVAPDTSPRGLNVEGEAD-SYDFGVGAGFYLNATQEKWKN-WRMYDYVVKELPKL 133
Query: 138 LAEEFGSKVDFFKNISITGHSMGGFGALSGYLKNINRYKSCSAFAPIVNPSAVPWGQKAF 197
L+E F S++D K SI+GHSMGG GAL+ YL+N+++YKS SAFAPI NP WGQKAF
Sbjct: 134 LSENF-SQLDTTK-ASISGHSMGGHGALTIYLRNLDKYKSVSAFAPITNPINCAWGQKAF 191
Query: 198 TGYLGSSNKEAWDGYDPTKLIKHVENLNGNKILIHVGSNDPFLEKHLRPHLLQEAAKGTF 257
T YLG NK AW+ YD T LI NL+ ILI G ND F L P +EA K
Sbjct: 192 TNYLG-DNKAAWEEYDATCLISKYNNLSAT-ILIDQGENDQFYPDQLLPSKFEEACKKV- 248
Query: 258 WEGKIEVNFEDGFDHSYYFISTFVPQHAEFHAKHLGL 294
+ + G+DHSYYFI+TF+ H HA+ L L
Sbjct: 249 -NAPLLLRLHPGYDHSYYFIATFIEDHISHHAQALEL 284
>CE25312 [R] KOG3101 Esterase D
Length = 280
Score = 217 bits (552), Expect = 2e-56
Identities = 131/295 (44%), Positives = 174/295 (58%), Gaps = 15/295 (5%)
Query: 1 MALKINSAIAVCGGKLLKLSHFSSCTGTDMDVNLYLPKQYYTSIDDSKLIPSVLYLSGLT 60
MA + S+ G+ H SS T +M +Y+P D KL P++ YLSGLT
Sbjct: 1 MAATLVSSNRSFKGRQFVYKHASSTTQCEMTFGVYIPDHK----PDEKL-PALFYLSGLT 55
Query: 61 CTPQNASEKAFWQIQADKFGFAVIFPDTSPRGDGVATDPEGSWDFGLGAGFYVNATEQPY 120
CT N EK+ +Q A K V+ PDTSPRG V D E SWDFG GAGFYVNAT + +
Sbjct: 56 CTHANFMEKSGFQQFASKHRLVVVHPDTSPRGVDVDGDSE-SWDFGKGAGFYVNATVEKW 114
Query: 121 SSHYKMYDYVHKELPEVLAEEFGSKVDFFKNISITGHSMGGFGALSGYLKNINRYKSCSA 180
+ +Y+MYDY+ KEL E + + +D K I I GHSMGG GAL+ L+N ++++S SA
Sbjct: 115 AKNYRMYDYIVKELLEEVVPSV-APIDLAK-IGIFGHSMGGHGALTIGLRNSSKFQSISA 172
Query: 181 FAPIVNPSAVPWGQKAFTGYLGSSNKEAWDGYDPTKLIKHVENLNGNKILIHVGSNDPFL 240
FAPI NP VPWGQKA TGYLG +K W+ YD ++++K +IL+ G+ D FL
Sbjct: 173 FAPICNPITVPWGQKALTGYLGDEDKSTWNQYDASEVLKAYSGPK-REILVDQGAADNFL 231
Query: 241 EKHLRPHLLQEAAKGTFWEGKIEVNFEDGFDHSYYFISTFVPQHAEFHAKHLGLI 295
+ L+P L+ +I V + FDHSYYFI++F+ H E HAK L I
Sbjct: 232 AQ-LKPETLKSKE-----NAEIVVRMQAEFDHSYYFIASFMADHFEHHAKILNKI 280
>Hs20547663 [R] KOG3101 Esterase D
Length = 282
Score = 216 bits (549), Expect = 4e-56
Identities = 134/294 (45%), Positives = 172/294 (57%), Gaps = 16/294 (5%)
Query: 1 MALKINSAIAVCGGKLLKLSHFSSCTGTDMDVNLYLPKQYYTSIDDSKLIPSVLYLSGLT 60
MALK S+ GG H S M +YLP + T P++ +LSGLT
Sbjct: 1 MALKQISSNKCFGGLQKVFEHDSVELNCKMKFAVYLPPKAETG-----KCPALYWLSGLT 55
Query: 61 CTPQNASEKAFWQIQADKFGFAVIFPDTSPRGDGVATDPEGSWDFGLGAGFYVNATEQPY 120
CT QN K+ + A + G VI PDTSPRG + + E SWDFG GAGFYV+ATE P+
Sbjct: 56 CTEQNFISKSGYHQSASEHGLVVIAPDTSPRGCNIKGEDE-SWDFGTGAGFYVDATEDPW 114
Query: 121 SSHYKMYDYVHKELPEVLAEEFGSKVDFFKNISITGHSMGGFGALSGYLKNINRYKSCSA 180
++Y+MY YV +ELP+++ F VD + +SI GHSMGG GAL LKN +YKS SA
Sbjct: 115 KTNYRMYSYVTEELPQLINANF--PVDP-QRMSIFGHSMGGHGALICALKNPGKYKSVSA 171
Query: 181 FAPIVNPSAVPWGQKAFTGYLGSSNKEAWDGYDPTKLIKHVENLNGNKILIHVGSNDPFL 240
FAPI NP PWG+KAF+GYLG +++ W YD T L+K + ILI G +D FL
Sbjct: 172 FAPICNPVLCPWGKKAFSGYLG-TDQSKWKAYDATHLVKSYPG-SQLDILIDQGKDDQFL 229
Query: 241 EKHLRPHLLQEAAKGTFWEGKIEVNF--EDGFDHSYYFISTFVPQHAEFHAKHL 292
L LL + E KI V F ++G+DHSYYFI+TF+ H HAK+L
Sbjct: 230 ---LDGQLLPDNFIAACTEKKIPVVFRLQEGYDHSYYFIATFITDHIRHHAKYL 280
>7300589 [R] KOG3101 Esterase D
Length = 286
Score = 192 bits (487), Expect = 6e-49
Identities = 123/296 (41%), Positives = 166/296 (55%), Gaps = 24/296 (8%)
Query: 1 MALKINSAIAVCGGKLLKLSHFSSCTGTDMDVNLYLPKQYYTSIDDSKLIPSVLYLSGLT 60
M L+ SA G+ H S DM ++LP + K P + +LSGLT
Sbjct: 1 MTLEQLSANKCFEGEQRVYRHRSDTLKCDMTFGVFLPPAAL----EGKPCPVLFFLSGLT 56
Query: 61 CTPQNASEKAFWQIQADKFGFAVIFPDTSPRGDGVATDPEGSWDFGLGAGFYVNATEQPY 120
CT +N +K+ +Q A + V+ PDTSPRG +A + ++DFG GAGFYV+A E+P+
Sbjct: 57 CTHENFIQKSGFQQHAARHNLIVVNPDTSPRGVEIAGQDD-AYDFGSGAGFYVDAKEEPW 115
Query: 121 SSHYKMYDYVHKELPEVLAEEF----GSKVDFFKNISITGHSMGGFGALSGYLKNINRYK 176
S HYKMY YV +EL +V+ G + I GHSMGG GAL LKN Y+
Sbjct: 116 SKHYKMYSYVTQELVDVVNANLPVVPGKR-------GIFGHSMGGHGALICALKNPGLYQ 168
Query: 177 SCSAFAPIVNPSAVPWGQKAFTGYLGSSNKEAWDGYDPTKLIKHVENLNGNKILIHVGSN 236
S SAFAPI NP+ PWG+KAF GYLG SN + W +D T L+ E+ ++ I G+
Sbjct: 169 SVSAFAPIANPTECPWGKKAFAGYLG-SNPDDWALWDATHLVSQYES-TPQELFIDQGAA 226
Query: 237 DPFLE-KHLRPHLLQEAAKGTFWEGKIEVNFE--DGFDHSYYFISTFVPQHAEFHA 289
D FL K L P L AA G I+ F+ +G+DHSY++I+TFV +H +HA
Sbjct: 227 DNFLAGKQLLPENLLAAADG---NDHIQTIFKQREGYDHSYFYIATFVAEHIAYHA 279
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.138 0.427
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,174,301
Number of Sequences: 60738
Number of extensions: 960075
Number of successful extensions: 1954
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1929
Number of HSP's gapped (non-prelim): 5
length of query: 295
length of database: 30,389,216
effective HSP length: 106
effective length of query: 189
effective length of database: 23,950,988
effective search space: 4526736732
effective search space used: 4526736732
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)