ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI0548 good R KOG2055 General function prediction only WD40 repeat protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI0548 183770 185467 566
(566 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJL069c [R] KOG2055 WD40 repeat protein 670 0.0
SPBC29A3.06 [R] KOG2055 WD40 repeat protein 337 2e-92
At5g14050 [R] KOG2055 WD40 repeat protein 198 2e-50
Hs7705765 [R] KOG2055 WD40 repeat protein 135 1e-31
CE00816 [R] KOG2055 WD40 repeat protein 127 6e-29
7302935 [R] KOG2055 WD40 repeat protein 126 1e-28
>YJL069c [R] KOG2055 WD40 repeat protein
Length = 594
Score = 670 bits (1728), Expect = 0.0
Identities = 335/590 (56%), Positives = 417/590 (69%), Gaps = 27/590 (4%)
Query: 1 MDHTIPPPDEEELVLSKLVFGDNSDFYNELQNINLEL----------SSEEDATEYVQXX 50
M+ ++PPPDEEE +L+K VFGD +D L N + E++ +E
Sbjct: 8 MNVSVPPPDEEEQLLAKFVFGDTTDLQENLAKFNADFIFNEQEMDVEDQEDEGSE--SDN 65
Query: 51 XXXXXXXXXXIGKVMDDQLFFVDDGELNVXXXXXXXXXXXXXQASGXXXXXXXXXXXXXA 110
+ V +DQLFFVDDG N A
Sbjct: 66 SEEDEAQNGELDHVNNDQLFFVDDGG-NEDSQDKNEDTMDVDDEDDSSSDDYSEDSEEAA 124
Query: 111 WVDSDDDRLQITVTENNRSKKLRKSHVESQINGREYLQRLRSQFEKIYPKPNWIXXXXXX 170
W+DSDD+++++ + N++KKLR S+ ES+ING Y+ RLRSQFEKIYP+P W+
Sbjct: 125 WIDSDDEKIKVPILVTNKTKKLRTSYNESKINGVHYINRLRSQFEKIYPRPKWVDDESDS 184
Query: 171 XXXXXXXXXXGDYDNVIDGDINALTKILERTYNY--TTANSKLLPPQTLDITRLKDANAS 228
+NVI+GDINALTKIL TYNY T +NSKLLPP+ LDI RLKDANAS
Sbjct: 185 ELDDEEDDEEEGSNNVINGDINALTKILSTTYNYKDTLSNSKLLPPKKLDIVRLKDANAS 244
Query: 229 HPSHSAIQSLSFHSTKPILLTGGYDRTLRIYHIDGKANHLITSVHIKGTPVQTCSFYSSQ 288
HPSHSAIQSLSFH +KP+LLTGGYD+TLRIYHIDGK NHL+TS+H+ G+P+QTC+FY+S
Sbjct: 245 HPSHSAIQSLSFHPSKPLLLTGGYDKTLRIYHIDGKTNHLVTSLHLVGSPIQTCTFYTSL 304
Query: 289 DGK-EQKVYTGGRRRYMHAWDLSNNN---TSVAKIDKISRLYGHQDTQRSFEKFKLAHFQ 344
+ +Q ++T GRRRYMH+WDLS N + AKI+K SRLYGH+ TQRSFE FK+AH Q
Sbjct: 305 SNQNQQNIFTAGRRRYMHSWDLSLENLTHSQTAKIEKFSRLYGHESTQRSFENFKVAHLQ 364
Query: 345 DAN-GARHGLICLQGNNGWVNILHSSTGVWLQGCKIEGAISDFCIDYKPQQNGHFHTILI 403
++ + HG++ LQGNNGW+NILHS++G+WL GCKIEG I+DFCIDY+P G F TILI
Sbjct: 365 NSQTNSVHGIVLLQGNNGWINILHSTSGLWLMGCKIEGVITDFCIDYQPISRGKFRTILI 424
Query: 404 ATNTYGEVWEFNLNENGNTVRKWKDDGGVGITKIQVGGGSTISSPK---KRIIPNRWIAI 460
A N YGEVWEF+LN+NG+ +R+WKD GGVGITKIQVGGG+T + P +I NRW+A+
Sbjct: 425 AVNAYGEVWEFDLNKNGHVIRRWKDQGGVGITKIQVGGGTTTTCPALQISKIKQNRWLAV 484
Query: 461 GSDSGFVNVYDRQ----SDSQRPVFTLDQLTTSISSLEFSPDGQMLCIASRATKDALRLV 516
GS+SGFVN+YDR S + PV LDQLTT+IS+L+FSPDGQ+LC+ASRA KDALRLV
Sbjct: 485 GSESGFVNLYDRNNAMTSSTPTPVAALDQLTTTISNLQFSPDGQILCMASRAVKDALRLV 544
Query: 517 HLATGTVFSNWPTSGTPLGKVTSVAFSPQGEMLAVGNEQGKVRLWRLNHY 566
HL + +VFSNWPTSGTPLGKVTSVAFSP G +LAVGNEQGKVRLW+LNHY
Sbjct: 545 HLPSCSVFSNWPTSGTPLGKVTSVAFSPSGGLLAVGNEQGKVRLWKLNHY 594
>SPBC29A3.06 [R] KOG2055 WD40 repeat protein
Length = 556
Score = 337 bits (865), Expect = 2e-92
Identities = 214/575 (37%), Positives = 310/575 (53%), Gaps = 77/575 (13%)
Query: 9 DEEELVLSKLVFGDNSDF----------YNELQNINLELSSEEDATEYVQXXXXXXXXXX 58
D EEL L VFG+ ++F ++ + N ELS + DA E
Sbjct: 4 DTEELELENAVFGNINNFSSFLDKENETFDVMMNEAPELSEDNDAQE------------- 50
Query: 59 XXIGKVMDDQLFFVDDGELNVXXXXXXXXXXXXXQASGXXXXXXXXXXXXXAWVDSDDDR 118
+ K+ D +LF D G + W DSDD+R
Sbjct: 51 DELEKLEDAELFMFDTGSADGAKDSVPLDIIAGDNT--VKEDEEASNEIPSIWEDSDDER 108
Query: 119 LQITVTENNRSKKLRKSHVESQINGREYLQRLRSQFEKIYPKPNWIXXXXXXXXXXXXXX 178
L I++ +++R +KLR+ E +NG +Y +RLR+QFE++YP P W
Sbjct: 109 LMISLQDHSRLRKLRQYEDEDMVNGLQYARRLRTQFERVYPVPEW-------AKKQDVTE 161
Query: 179 XXGDYDNVIDGDI--NALTKILERTYNYTTANSKLLPPQTLDITRLKDANASHPSHSAIQ 236
+++ + + + +L + + + +Y +SKLL P T++I RLKDAN PSHS I+
Sbjct: 162 EEDEFNALSEKSVIPKSLKSLFKSSVSYINQSSKLLAPGTINIKRLKDANFQAPSHSGIR 221
Query: 237 SLSFHSTKPILLTGGYDRTLRIYHIDGKANHLITSVHIKGTPVQTCSFYSSQDGKEQKVY 296
+S H P+LLT G+DRTLRIY +DGK N L+TS+H++ + +QT F+ DGK +V
Sbjct: 222 CMSIHPYFPLLLTCGFDRTLRIYQLDGKVNPLVTSLHLRSSALQTALFH--PDGK--RVI 277
Query: 297 TGGRRRYMHAWDLSNNNTSVAKIDKISRLYGHQDTQRSFEKFKLAHFQDANGARHGLICL 356
GRR+YM+ WDL + A++ K+SR+YG ++ Q S E+F + D G I L
Sbjct: 278 AAGRRKYMYIWDLES-----AQVQKVSRMYGQENFQPSMERFHV----DPTGK---YIAL 325
Query: 357 QGNNGWVNILHSSTGVWLQGCKIEGAISDFCIDYKPQQNGHFHTILIATNTYG-EVWEFN 415
+G +G +N+LH+ TG + KIEG +SD +G +L +YG EVW FN
Sbjct: 326 EGRSGHINLLHALTGQFATSFKIEGVLSDVLF----TSDGSEMLVL----SYGAEVWHFN 377
Query: 416 LNENGNTVRKWKDDGGVGITKIQVGGGSTISSPKKRIIPNRWIAIGSDSGFVNVYDRQSD 475
+ E + VR+W+ GV T + N+++AIGS SG VN+YD Q+
Sbjct: 378 V-EQRSVVRRWQVQDGVSTTHFCLDPS------------NKYLAIGSKSGIVNIYDLQTS 424
Query: 476 ----SQRPVFTLDQLTTSISSLEFSPDGQMLCIASRATKDALRLVHLATGTVFSNWPTSG 531
+ +PV TLD +T SI+S+ FS D Q+L IASR KD LRLVH+ + +VF NWPTS
Sbjct: 425 NADAAPKPVTTLDNITFSINSMSFSQDSQVLAIASRGKKDTLRLVHVPSFSVFRNWPTSA 484
Query: 532 TPLGKVTSVAFSPQGEMLAVGNEQGKVRLWRLNHY 566
TPLG+VT +AF GE L VGNE G+V LW+L HY
Sbjct: 485 TPLGRVTCLAFGKGGE-LCVGNEAGRVGLWKLAHY 518
>At5g14050 [R] KOG2055 WD40 repeat protein
Length = 546
Score = 198 bits (504), Expect = 2e-50
Identities = 137/460 (29%), Positives = 229/460 (49%), Gaps = 48/460 (10%)
Query: 110 AWVDSDDDRLQITVTENNRSKKLRKSHVESQINGREYLQRLRSQFEKIYPKPNWIXXXXX 169
AW D ++ ++ + + NR +KLRK E I+G EY+ RLR+ K+ P +W
Sbjct: 129 AWEDEEEKQINVDIASVNRLRKLRKEENEGLISGSEYIARLRAHHAKLNPGTDW-----A 183
Query: 170 XXXXXXXXXXXGDYDNVIDGDINALTKILERTYNYTTANSKLLPPQTLDITRLKDANASH 229
D D+ DG ++ + + E + N L L+ ++L DANA+
Sbjct: 184 RPDSQIVDGESSDDDDTQDGGVDDILRTNEDLVVKSRGNK--LCAGRLEYSKLVDANAAD 241
Query: 230 PSHSAIQSLSFHSTKPILLTGGYDRTLRIYHIDGKANHLITSVHIKGTPVQTCSFYSSQD 289
PS+ I S+ FH +LLT G DR LR + IDGK N I S+ ++ P++ +F +
Sbjct: 242 PSNGPINSVHFHQNAQLLLTAGLDRRLRFFQIDGKRNTKIQSIFLEDCPIRKAAFLPNGS 301
Query: 290 GKEQKVYTGGRRRYMHAWDLSNNNTSVAKIDKISRLYGHQDTQRSFEKFKLAHFQDANGA 349
+V GRR++ +++DL AK DKI L G ++ +S E F+++ QD+N
Sbjct: 302 ----QVIVSGRRKFFYSFDLEK-----AKFDKIGPLVGREE--KSLEYFEVS--QDSN-- 346
Query: 350 RHGLICLQGNNGWVNILHSSTGVWLQGCKIEGAISDFCIDYKPQQNGHFHTILIATNTYG 409
I GN G++ ++ + T + K+ G++ + H L+++ G
Sbjct: 347 ---TIAFVGNEGYILLVSTKTKELIGTLKMNGSVRSLAFS---EDGKH----LLSSGGDG 396
Query: 410 EVWEFNLNENGNTVRKWKDDGGVGITKIQVGGGSTISSPKKRIIPNRWIAIGSDSGFVNV 469
+V+ ++L + K D+G G S SS + A G+D G VN+
Sbjct: 397 QVYVWDLRTM-KCLYKGVDEGSTC-------GTSLCSS-----LNGALFASGTDRGIVNI 443
Query: 470 YDRQS---DSQRPVFTLDQLTTSISSLEFSPDGQMLCIASRATKDALRLVHLATGTVFSN 526
Y + ++P+ T+D LT+ I ++F+ D Q+L I S K++++LVH+ + TVFSN
Sbjct: 444 YKKSEFVGGKRKPIKTVDNLTSKIDFMKFNHDAQILAIVSTMNKNSVKLVHVPSLTVFSN 503
Query: 527 WPTSGTPLGKVTSVAFSPQGEMLAVGNEQGKVRLWRLNHY 566
WP + + + FSP +A+GN GKV L++L+HY
Sbjct: 504 WPPPNSTMHYPRCLDFSPGSGFMAMGNAAGKVLLYKLHHY 543
>Hs7705765 [R] KOG2055 WD40 repeat protein
Length = 521
Score = 135 bits (341), Expect = 1e-31
Identities = 119/457 (26%), Positives = 220/457 (48%), Gaps = 64/457 (14%)
Query: 111 WVDSDDDRLQITVTENNRSKK-LRKSHVESQINGREYLQRLRSQFEKIYPK-PNWIXXXX 168
WVD +D+ ++ NNR +K + K+ ES+++ +RL+ +F+ P W
Sbjct: 118 WVDEEDEDEEMVDMMNNRFRKDMMKNASESKLSKDNLKKRLKEEFQHAMGGVPAW----- 172
Query: 169 XXXXXXXXXXXXGDYDNVIDGDINALTKILERTYNYTTANSKLLPPQTLDITRLKDANAS 228
D ++ D D +L+RT N+ + ++ L P L + + ANA
Sbjct: 173 ---AETTKRKTSSDDESEEDED-----DLLQRTGNFISTSTSL-PRGILKMKNCQHANAE 223
Query: 229 HPSHSAIQSLSFHSTKPILLTGGYDRTLRIYHIDGKANHLITSVHIKGTPVQTCSFYSSQ 288
P+ + I S+ FH I++ G D + ++ +DGK N I S++++ P+ F S
Sbjct: 224 RPTVARISSVQFHPGAQIVMVAGLDNAVSLFQVDGKTNPKIQSIYLERFPIFKACF--SA 281
Query: 289 DGKEQKVYTGGRRRYMHAWDLSNNNTSVAKIDKISRLYGHQDT-QRSFEKFKLAHFQDAN 347
+G E+ + T + ++ +D+ K+ + ++ G ++ RSFE F
Sbjct: 282 NG-EEVLATSTHSKVLYVYDML-----AGKLIPVHQVRGLKEKIVRSFEVSPDGSF---- 331
Query: 348 GARHGLICLQGNNGWVNILHSSTGVWLQGCKIEG--AISDFCIDYKPQQNGHFHTILIAT 405
+ + G G++++L T + KI G A S F D K + A+
Sbjct: 332 ------LLINGIAGYLHLLAMKTKELIGSMKINGRVAASTFSSDSKK---------VYAS 376
Query: 406 NTYGEVWEFNLNENGNTVRKWKDDGGVGITKIQVGGGSTISSPKKRIIPNRWIAIGSDSG 465
+ GEV+ +++N + ++ D+G + G +I++ + +++A GS+ G
Sbjct: 377 SGDGEVYVWDVNSR-KCLNRFVDEGSLY--------GLSIATSRN----GQYVACGSNCG 423
Query: 466 FVNVYDRQSDSQ----RPVFTLDQLTTSISSLEFSPDGQMLCIASRATKDALRLVHLATG 521
VN+Y++ S Q +P+ + L T ++SL F+P ++L IAS K+A+RLVHL +
Sbjct: 424 VVNIYNQDSCLQETNPKPIKAIMNLVTGVTSLTFNPTTEILAIASEKMKEAVRLVHLPSC 483
Query: 522 TVFSNWPT-SGTPLGKVTSVAFSPQGEMLAVGNEQGK 557
TVFSN+P + V ++ FSP+ A+GNE+ +
Sbjct: 484 TVFSNFPVIKNKNISHVHTMDFSPRSGYFALGNEKAR 520
>CE00816 [R] KOG2055 WD40 repeat protein
Length = 429
Score = 127 bits (318), Expect = 6e-29
Identities = 106/471 (22%), Positives = 191/471 (40%), Gaps = 71/471 (15%)
Query: 110 AWVDSDDDRLQITVTENNRSK-----KLRKSHVESQINGREYLQRLRSQFEKIYP-KPNW 163
AW D DDD + + V + + K +++ +EY+ RL+ F K + P W
Sbjct: 13 AWHDEDDDNMVVAVPKKVKMTMRVELKRTTDEEHGELDSKEYVGRLQEAFRKRHGGTPKW 72
Query: 164 IXXXXXXXXXXXXXXXXGDYDNVIDGDINALTKILERTYNYTTANSKLLPPQTLDITRLK 223
Y + D+N LP T+ T +K
Sbjct: 73 AKAAAGDEEEGSLLKTAAGY---LAKDVN-------------------LPKTTIHTTLIK 110
Query: 224 DANASHPSHSAIQSLSFHSTKPILLTGGYDRTLRIYHI--DGKANHLITSVHIKGTPVQT 281
D N H I + FH T+P+L+ ++++ + + K + + S + P+ +
Sbjct: 111 DFNIGHRYTRGITVVKFHKTRPVLIVADQGGNVQLFKVSREVKKDRFLQSANFSKFPIDS 170
Query: 282 CSFYSSQDGKEQKVYTGGRRRYMHAWDLSNNNTSVAKIDKISRLYGHQDTQRSFEKFKLA 341
D + + R+ Y+ +++ + K G F ++
Sbjct: 171 LEI---ADKGHSVICSSSRKEYLMQYNMETREVTQLKPPNTVPKQG-------IRLFAIS 220
Query: 342 HFQDANGARHGLICLQGNNGWVNILHSSTGVWLQGCKIEGAISDFCIDYKPQQNGHFHTI 401
H + + G+N + +LH+++ + + S+ I + P H I
Sbjct: 221 HDSQ-------FLAIAGHNSHIYVLHATSMEHITTISLPANASE--IKFFPS---HSREI 268
Query: 402 LIATNTYGEVWEFNLNENG--NTVRKWKDDGGVGITKIQVGGGSTISSPKKRIIPNRWIA 459
I T G++ N+ G ++ + DDG V T + + + A
Sbjct: 269 WIICET-GQIVIANIGLPGTKSSQHTFTDDGAVHGTTLAISQHGD------------YFA 315
Query: 460 IGSDSGFVNVYD----RQSDSQRPVFTLDQLTTSISSLEFSPDGQMLCIASRATKDALRL 515
GSD+G VNVY R S + RP+F + L T++SS+ F+ D Q++ I S + LRL
Sbjct: 316 TGSDTGIVNVYSGNDCRNSTNPRPLFNVSNLVTAVSSIAFNSDAQLMAICSNVKDNHLRL 375
Query: 516 VHLATGTVFSNWPTSGTPLGKVTSVAFSPQGEMLAVGNEQGKVRLWRLNHY 566
VH+A+ T F N+P + V FSP G +AVGN+ G++ ++ ++H+
Sbjct: 376 VHVASQTTFKNFPERNGKVTHARCVEFSPNGGYMAVGNDDGRLHVFEIHHF 426
>7302935 [R] KOG2055 WD40 repeat protein
Length = 506
Score = 126 bits (316), Expect = 1e-28
Identities = 120/463 (25%), Positives = 202/463 (42%), Gaps = 65/463 (14%)
Query: 110 AWVDSDDDRLQITVTENNRSKKLRKSHVESQINGREYLQRLRSQFEKIYPKPNWIXXXXX 169
AW DSDD+ LQ+ + +H+ + +EYL ++F++ +P W
Sbjct: 100 AWSDSDDEDLQVGDVKKATKHTGPLNHLRKDKSYKEYLT---ARFQRTLNQPKWAEKKVK 156
Query: 170 XXXXXXXXXXXGDYDNVIDGDINALTKILERTYNYTT--ANSKLLPPQTLDITRLKDANA 227
N D D+++ ++L RT + A + LP +TL+ R+KD N
Sbjct: 157 ---------------NEDDEDVSSDEELL-RTVGFIDRKARNSDLPQKTLNFKRVKDLNR 200
Query: 228 SHPSHSAIQSLSFHSTKPILLTGGYDRTLRIYHIDGKANHLITSVHIKGTPVQTCSFYSS 287
+ + S+ FH T L G + IY +DG+ N + ++ K P+ CS +
Sbjct: 201 ATYAEGNATSIQFHPTSTAALVAGMNGLATIYAVDGQKNERLHNMRFKKFPL-ACSRIAP 259
Query: 288 QDGKEQKVYTGGRRRYMHAWDLSNNNTSVAKIDKISRLYGHQDTQRSFEKFKLAHFQDAN 347
+ + G + + +++DL AK K+ +L G + FE F
Sbjct: 260 CG---TRAFFGSVKPFYYSYDLLE-----AKESKL-KLPGAMEFMHRFEVSPCGKF---- 306
Query: 348 GARHGLICLQGNNGWVNILHSSTGVWLQGCKIEGAISDFCIDYKPQQNGHFHTILIATNT 407
I G G +++L + T L K EG + F ++ IL+ +T
Sbjct: 307 ------IVTAGKFGAIHLLTAKTNELLHSFKQEGKVKGFTWSSDSKR------ILVCGST 354
Query: 408 YGEVWEFNLNENGNTVRKWKDDGGVGITKIQVGGGSTISSPKKRIIPNRWIAIGSDSGFV 467
V NL +N + DDG + IQ+ SP +R++ A GS G V
Sbjct: 355 -SNVSVLNLRQN-LIEHIFMDDGCIHGESIQL-------SPNQRLL-----ATGSQEGVV 400
Query: 468 NVYDRQS----DSQRPVFTLDQLTTSISSLEFSPDGQMLCIASRATKDALRLVHLATGTV 523
N+YD +S + +P L T+I+ L+F+ ++L + S +A +L H + TV
Sbjct: 401 NIYDYESIFASKAPQPEKRFMNLRTAITDLQFNHSSELLAMCSSEAPNAFKLAHFPSATV 460
Query: 524 FSNWPTSGTPLGKVTSVAFSPQGEMLAVGNEQGKVRLWRLNHY 566
+SN+P +G VTS+AFSP LA + +V L+RL ++
Sbjct: 461 YSNFPAQNEKVGFVTSMAFSPHSSFLAFATKGKQVPLFRLKYF 503
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 33,162,834
Number of Sequences: 60738
Number of extensions: 1376384
Number of successful extensions: 5151
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 5108
Number of HSP's gapped (non-prelim): 12
length of query: 566
length of database: 30,389,216
effective HSP length: 112
effective length of query: 454
effective length of database: 23,586,560
effective search space: 10708298240
effective search space used: 10708298240
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)