ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI0561 good R KOG3178 General function prediction only Hydroxyindole-O-methyltransferase and related SAM-dependent methyltransferases
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI0561 189413 190093 227
(227 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YBR261c [R] KOG3178 Hydroxyindole-O-methyltransferase and relate... 311 6e-85
SPAC16E8.14c [R] KOG3178 Hydroxyindole-O-methyltransferase and r... 193 2e-49
Hs7661528 [R] KOG3178 Hydroxyindole-O-methyltransferase and rela... 158 5e-39
7303836 [R] KOG3178 Hydroxyindole-O-methyltransferase and relate... 147 9e-36
CE28146 [R] KOG3178 Hydroxyindole-O-methyltransferase and relate... 144 1e-34
At5g44450 [R] KOG3178 Hydroxyindole-O-methyltransferase and rela... 127 1e-29
Hs18548761 [R] KOG3178 Hydroxyindole-O-methyltransferase and rel... 97 2e-20
>YBR261c [R] KOG3178 Hydroxyindole-O-methyltransferase and related
SAM-dependent methyltransferases
Length = 232
Score = 311 bits (796), Expect = 6e-85
Identities = 152/223 (68%), Positives = 179/223 (80%), Gaps = 3/223 (1%)
Query: 5 DSNINYKDAIDYWTSIPATVDGVLGGYGDQTPLPNMDVHGSMHFVRKLKSRMITE--GPK 62
DS+I Y+DAIDYWT + ATVDGVLGGYG+ T +P MDV GS +F+RKLKSRM+ + K
Sbjct: 6 DSHIKYEDAIDYWTDVDATVDGVLGGYGEGTVVPTMDVLGSNNFLRKLKSRMLPQENNVK 65
Query: 63 NSCDIGAGIGRVTKNFLSKISDKVDLVEPVVPFVEQAHVELDALKKEGKIGEIFAIGMQD 122
+ DIGAGIGRV+K L K + K+DLVEPV PF+EQ HVEL LK +G+IG+I+ +GMQD
Sbjct: 66 YAVDIGAGIGRVSKTMLHKHAAKIDLVEPVKPFIEQMHVELAELKDKGQIGQIYEVGMQD 125
Query: 123 WNPEEGKYWLIWCQWCVGHLPDEELIQFFHRCKKGLQKNGTIVVKENNTVGD-DDFDPVD 181
W P+ GKYWLIWCQWCVGHLPD EL+ F RC GLQ NGTIVVKENNT D DDFD D
Sbjct: 126 WTPDAGKYWLIWCQWCVGHLPDAELVAFLKRCIVGLQPNGTIVVKENNTPTDTDDFDETD 185
Query: 182 SSVTRSNEKFMQLFKEAGLKVIATERQRGLPDGLYPVQMYALK 224
SSVTRS+ KF Q+F+EAGLK+IA+ERQRGLP LYPV+MYALK
Sbjct: 186 SSVTRSDAKFRQIFEEAGLKLIASERQRGLPRELYPVRMYALK 228
>SPAC16E8.14c [R] KOG3178 Hydroxyindole-O-methyltransferase and related
SAM-dependent methyltransferases
Length = 219
Score = 193 bits (490), Expect = 2e-49
Identities = 101/214 (47%), Positives = 132/214 (61%), Gaps = 6/214 (2%)
Query: 10 YKDAIDYWTSIPATVDGVLGGYGDQTPLPNMDVHGSMHFVRKLKSRMITEGPKNSCDIGA 69
Y DAIDYW + TVDG+LGG G +P DV GS F+ +L R+ + D GA
Sbjct: 7 YSDAIDYWNGVQPTVDGMLGGLGTGR-IPQTDVVGSRTFLNRLNYRIGKIENLVAADCGA 65
Query: 70 GIGRVTKNFLSKISDKVDLVEPVVPFVEQAHVELDALKKEGKIGEIFAIGMQDWNPEEGK 129
GIGRVT+N L KI+ VDLVEPV F+ A +L K +G+Q+W PE+ +
Sbjct: 66 GIGRVTENVLLKIASHVDLVEPVENFISTAKKQLAT-----KPCSFINVGLQNWTPEKNR 120
Query: 130 YWLIWCQWCVGHLPDEELIQFFHRCKKGLQKNGTIVVKENNTVGDDDFDPVDSSVTRSNE 189
Y LIW QWC+ HL DE+LI + RC + +Q+ G I VKEN + +D FDP+DSSVTR +
Sbjct: 121 YGLIWNQWCLSHLTDEDLIAYLSRCCEAIQEKGVICVKENVSSFEDTFDPIDSSVTRCEQ 180
Query: 190 KFMQLFKEAGLKVIATERQRGLPDGLYPVQMYAL 223
LFK+A L V+A Q G P+ L+PV+MYAL
Sbjct: 181 SLKSLFKKANLVVVAETLQHGFPEELFPVKMYAL 214
>Hs7661528 [R] KOG3178 Hydroxyindole-O-methyltransferase and related
SAM-dependent methyltransferases
Length = 224
Score = 158 bits (400), Expect = 5e-39
Identities = 93/221 (42%), Positives = 125/221 (56%), Gaps = 15/221 (6%)
Query: 10 YKDAIDYWTSIPATVDGVLGGYGDQTPLPNMDVHGSMHFVRKLKSRMITEGPKN---SC- 65
Y A YW IP TVDG+LGGYG + ++D++ S RK R + EGP SC
Sbjct: 13 YSKAKTYWKQIPPTVDGMLGGYGHISS--SIDINSS----RKFLQRFLREGPNKTGTSCA 66
Query: 66 -DIGAGIGRVTKNFLSKISDKVDLVEPVVPFVEQAHVELDALKKEGK-IGEIFAIGMQDW 123
D GAGIGR+TK L + +VD+V+ F+ QA L +EGK + F G+QD+
Sbjct: 67 LDCGAGIGRITKRLLLPLFREVDMVDITEDFLVQAKTYLG---EEGKRVRNYFCCGLQDF 123
Query: 124 NPEEGKYWLIWCQWCVGHLPDEELIQFFHRCKKGLQKNGTIVVKENNTVGDDDFDPVDSS 183
PE Y +IW QW +GHL D+ L +F RCK L+ NG IV+K+N D VDSS
Sbjct: 124 TPEPDSYDVIWIQWVIGHLTDQHLAEFLRRCKGSLRPNGIIVIKDNMAQEGVILDDVDSS 183
Query: 184 VTRSNEKFMQLFKEAGLKVIATERQRGLPDGLYPVQMYALK 224
V R + ++ AGL ++A ERQ LPD +Y V +AL+
Sbjct: 184 VCRDLDVVRRIICSAGLSLLAEERQENLPDEIYHVYSFALR 224
>7303836 [R] KOG3178 Hydroxyindole-O-methyltransferase and related
SAM-dependent methyltransferases
Length = 276
Score = 147 bits (372), Expect = 9e-36
Identities = 86/228 (37%), Positives = 129/228 (55%), Gaps = 11/228 (4%)
Query: 1 MTTPDSNINYKDAIDYWTSIPATVDGVLGGYGDQTPLPNMDVHGSMHFVRKLKSRMITEG 60
+ P+S Y A YW+ +PATV+G+LGG G + +D+ GS F+R+++ G
Sbjct: 55 VAAPESEF-YNKAQKYWSEVPATVNGMLGGLGY---ISAIDIQGSNVFLREIR----VPG 106
Query: 61 PKNSCDIGAGIGRVTKNFLSKISDKVDLVEPVVPFVEQAHVELDALK-KEGKIGEIFAIG 119
+ + D GAGIGRVT+N L VDLVE F ++A + GK+G+I+ +G
Sbjct: 107 NRLALDCGAGIGRVTRNLLIPRFSCVDLVEQDPAFADKAREYCTSEDGSRGKVGQIYNVG 166
Query: 120 MQDWNPEEGKYWLIWCQWCVGHLPDEELIQFFHRCKKGLQKNGTIVVKENNTVGDDDF-D 178
+Q + P + +Y L+W QW +GHL D +L+ FF R K+GL + +KEN + D
Sbjct: 167 LQKFTPTQ-QYDLVWTQWVLGHLTDRDLVSFFRRIKQGLAPGAFLCLKENVSSSKKTVED 225
Query: 179 PVDSSVTRSNEKFMQLFKEAGLKVIATERQRGLPDGLYPVQMYALKTV 226
DSSVTR + + KEAG +++ +Q+ P GL+PV M A K V
Sbjct: 226 RNDSSVTRPLDSYEHFLKEAGFRIVRKVKQQNFPKGLFPVYMIACKPV 273
>CE28146 [R] KOG3178 Hydroxyindole-O-methyltransferase and related
SAM-dependent methyltransferases
Length = 234
Score = 144 bits (362), Expect = 1e-34
Identities = 82/216 (37%), Positives = 122/216 (55%), Gaps = 7/216 (3%)
Query: 10 YKDAIDYWTSIPATVDGVLGGYGDQTPLPNMDVHGSMHFVRKLKSRMITEGPKNSCDIGA 69
Y+ A +YW+ V+G+LGG+ L D+ S F+ LK + + + D GA
Sbjct: 16 YEKAEEYWSRASQDVNGMLGGF---EALHAPDISASKRFIEGLKKKNLFGYFDYALDCGA 72
Query: 70 GIGRVTKNFLSKISDKVDLVEPVVPFVEQAHVELDALKKEGKIGEIFAIGMQDWNPEEGK 129
GIGRVTK+ L KVD+ + V + ++ + K +IG+ F G+Q + P E +
Sbjct: 73 GIGRVTKHLLMPFFSKVDMEDVVEELITKSD---QYIGKHPRIGDKFVEGLQTFAPPERR 129
Query: 130 YWLIWCQWCVGHLPDEELIQFFHRCKKGLQKNGTIVVKENNTVGDDD-FDPVDSSVTRSN 188
Y LIW QW GHL DE+L+ FF RC KGL+ G IV+K+N T + FD D S TR+
Sbjct: 130 YDLIWIQWVSGHLVDEDLVDFFKRCAKGLKPGGCIVLKDNVTNHEKRLFDDDDHSWTRTE 189
Query: 189 EKFMQLFKEAGLKVIATERQRGLPDGLYPVQMYALK 224
+ ++ F ++ L +++ Q G P +YPV+MYALK
Sbjct: 190 PELLKAFADSQLDMVSKALQTGFPKEIYPVKMYALK 225
>At5g44450 [R] KOG3178 Hydroxyindole-O-methyltransferase and related
SAM-dependent methyltransferases
Length = 252
Score = 127 bits (320), Expect = 1e-29
Identities = 77/222 (34%), Positives = 118/222 (52%), Gaps = 19/222 (8%)
Query: 10 YKDAIDYWTSIPATVDGVLGGYGDQTPLPNMDVHGSMHFVRKL-KSRMITEGPKN----S 64
Y+D + YW + A+VDGVLGGYG + + D+ GS F++ L + R++ N +
Sbjct: 18 YRDGVSYWEGVEASVDGVLGGYGH---VNDADIIGSEVFLKTLLQERLVNNVGANQHLVA 74
Query: 65 CDIGAGIGRVTKNFLSKISDKVDLVEPVVPFVEQAHVELDALKKEG-KIGEIFAIGMQDW 123
D G+GIGR+TKN L + ++VDL+EPV F++ A L + E K F + +Q++
Sbjct: 75 LDCGSGIGRITKNLLIRYFNEVDLLEPVAQFLDAARENLASAGSETHKATNFFCVPLQEF 134
Query: 124 NPEEGKYWLIWCQWCVGHLPDEELIQFFHRCKKGLQKNGTIVVKENNTVGDDDFDPVD-- 181
P G+Y +IW QWC+GHL D + + FF+R K I + + F V
Sbjct: 135 TPAAGRYDVIWVQWCIGHLTDNDFVSFFNRAKDLFWIKRIIASPDRIHTSNSSFANVGYI 194
Query: 182 SSVTRSNEKFMQLFKEAGLKVIATERQRGLPDGLYPVQMYAL 223
S R+ + F+Q + Q+GLP L+ V+MYAL
Sbjct: 195 SIEQRNLQSFLQNHN--------FQDQKGLPQELFAVKMYAL 228
>Hs18548761 [R] KOG3178 Hydroxyindole-O-methyltransferase and related
SAM-dependent methyltransferases
Length = 303
Score = 96.7 bits (239), Expect = 2e-20
Identities = 64/214 (29%), Positives = 103/214 (47%), Gaps = 36/214 (16%)
Query: 10 YKDAIDYWTSIPATVDGVLGGYGDQTPLPNMDVHGSMHFVRKLKSRMITEGPKNSCDIGA 69
Y A ++ +PAT +G++G + + L + D+ S F+RK G + D G+
Sbjct: 120 YARAKLFYQEVPATEEGMMGNFIE---LSSPDIQASQKFLRKFVGGPGRAGTDCALDCGS 176
Query: 70 GIGRVTKNFLSKISDKVDLVEPVVPFVEQAHVELDALKKEGKIGEIFAIGMQDWNPEEGK 129
GIGRV+K+ L + + V+LV+ + F+ +A L
Sbjct: 177 GIGRVSKHVLLPVFNSVELVDMMESFLLEAQNYLQVK----------------------- 213
Query: 130 YWLIWCQWCVGHLPDEELIQFFHRCKKGLQKNGTIVVKENNTVGDDDFDPVDSSVTRSNE 189
GHL D++L+ F RC+ GL++NG I++K+N D DSSVTR +
Sbjct: 214 ----------GHLTDKDLLAFLSRCRDGLKENGIIILKDNVAREGCILDLSDSSVTRDMD 263
Query: 190 KFMQLFKEAGLKVIATERQRGLPDGLYPVQMYAL 223
L +++GL V+ E+Q G P+ PV M+AL
Sbjct: 264 ILRSLIRKSGLVVLGQEKQDGFPEQCIPVWMFAL 297
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.138 0.427
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,807,209
Number of Sequences: 60738
Number of extensions: 666188
Number of successful extensions: 1529
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1505
Number of HSP's gapped (non-prelim): 8
length of query: 227
length of database: 30,389,216
effective HSP length: 103
effective length of query: 124
effective length of database: 24,133,202
effective search space: 2992517048
effective search space used: 2992517048
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)