ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI0684 good C KOG1664 Energy production and conversion Vacuolar H+-ATPase V1 sector, subunit E
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI0684 231302 230616 -229
(229 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR332w [C] KOG1664 Vacuolar H+-ATPase V1 sector subunit E 219 2e-57
SPAC11E3.07 [C] KOG1664 Vacuolar H+-ATPase V1 sector subunit E 115 4e-26
Hs18087815 [C] KOG1664 Vacuolar H+-ATPase V1 sector subunit E 96 5e-20
7296718 [C] KOG1664 Vacuolar H+-ATPase V1 sector subunit E 94 2e-19
Hs4502317 [C] KOG1664 Vacuolar H+-ATPase V1 sector subunit E 91 2e-18
CE19362 [C] KOG1664 Vacuolar H+-ATPase V1 sector subunit E 87 1e-17
At3g08560 [C] KOG1664 Vacuolar H+-ATPase V1 sector subunit E 77 2e-14
At4g11150 [C] KOG1664 Vacuolar H+-ATPase V1 sector subunit E 76 4e-14
At1g64200 [C] KOG1664 Vacuolar H+-ATPase V1 sector subunit E 75 6e-14
Hs18549288 [C] KOG1664 Vacuolar H+-ATPase V1 sector subunit E 62 7e-10
>YOR332w [C] KOG1664 Vacuolar H+-ATPase V1 sector subunit E
Length = 233
Score = 219 bits (558), Expect = 2e-57
Identities = 119/231 (51%), Positives = 150/231 (64%), Gaps = 3/231 (1%)
Query: 2 STITALSPNQVNDELQKMQXXXXXXXXXXXXXXXXXXQQEYEIEKTGLVRNETSAIDNNI 61
S ITAL+PNQVNDEL KMQ QEYEIEKT +VRNET+ ID N
Sbjct: 3 SAITALTPNQVNDELNKMQAFIRKEAEEKAKEIQLKADQEYEIEKTNIVRNETNNIDGNF 62
Query: 62 ASRMKKAALKQQIVKSTIANKMRLKVLGTREEVLDSIFEKTKAELKQISSKKEEYKPXXX 121
S++KKA L QQI KSTIANKMRLKVL RE+ LD IFE+TK +L I++ ++EYKP
Sbjct: 63 KSKLKKAMLSQQITKSTIANKMRLKVLSAREQSLDGIFEETKEKLSGIANNRDEYKPILQ 122
Query: 122 XXXXXXXXXXXXPSAIIKVRETDVEIIESLVDDVAKEYEEKTGK-PI-KIELSSSYLNKD 179
P AI+K E DV++IES+ DD+ +EY EK + P+ +I +S+ YLNKD
Sbjct: 123 SLIVEALLKLLEPKAIVKALERDVDLIESMKDDIMREYGEKAQRAPLEEIVISNDYLNKD 182
Query: 180 -IAGGVIVSNGNGRIEVDNTXXXXXXXXXXXXXPAIRLELFGPSKTRKFFD 229
++GGV+VSN + +IE++NT PAIRLEL+GPSKTRKFFD
Sbjct: 183 LVSGGVVVSNASDKIEINNTLEERLKLLSEEALPAIRLELYGPSKTRKFFD 233
>SPAC11E3.07 [C] KOG1664 Vacuolar H+-ATPase V1 sector subunit E
Length = 227
Score = 115 bits (289), Expect = 4e-26
Identities = 66/225 (29%), Positives = 108/225 (47%), Gaps = 4/225 (1%)
Query: 6 ALSPNQVNDELQKMQXXXXXXXXXXXXXXXXXXQQEYEIEKTGLVRNETSAIDNNIASRM 65
+LS QV E+ KM ++E+++EK +VR + AID ++
Sbjct: 2 SLSDEQVQAEMHKMVSFIKQEALEKAKEIHTLSEEEFQVEKAKIVREQCDAIDQTYDMKL 61
Query: 66 KKAALKQQIVKSTIANKMRLKVLGTREEVLDSIFEKTKAELKQISSKKEEYKPXXXXXXX 125
K+A++ Q+I KS + NK RL++L ++++V+D IF + + +L I KK+ Y
Sbjct: 62 KRASMAQKIAKSNVLNKSRLEILNSKQKVIDDIFSRVEKKLDGIEQKKDAYTKFMADLIV 121
Query: 126 XXXXXXXXPSAIIKVRETDVEIIESLVDDVAKEYEEKTGKPIKIEL---SSSYLNKDIAG 182
P I+ R+ D EI+++ + + + K G I EL + +LN + G
Sbjct: 122 QAMELLGEPVGIVYSRQRDAEIVKAAIPKATEVLKSKNGS-IDYELDAETDDFLNDSVLG 180
Query: 183 GVIVSNGNGRIEVDNTXXXXXXXXXXXXXPAIRLELFGPSKTRKF 227
GV++ G+I VDNT P IR LFG + RKF
Sbjct: 181 GVVLVGLGGKIRVDNTLRARLEIVKEEALPEIRRLLFGENPNRKF 225
>Hs18087815 [C] KOG1664 Vacuolar H+-ATPase V1 sector subunit E
Length = 226
Score = 95.5 bits (236), Expect = 5e-20
Identities = 61/224 (27%), Positives = 102/224 (45%), Gaps = 1/224 (0%)
Query: 6 ALSPNQVNDELQKMQXXXXXXXXXXXXXXXXXXQQEYEIEKTGLVRNETSAIDNNIASRM 65
ALS V +++ M ++E+ IEK LV+ + I +
Sbjct: 2 ALSDVDVKKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKKE 61
Query: 66 KKAALKQQIVKSTIANKMRLKVLGTREEVLDSIFEKTKAELKQISSKKEEYKPXXXXXXX 125
K+ +++I+ ST+ N+ RLKVL R +++ + + K L +I E Y+
Sbjct: 62 KQIEQQKKILMSTMRNQARLKVLRARNDLISDLLSEAKLRLSRIVEDPEVYQGLLDKLVL 121
Query: 126 XXXXXXXXPSAIIKVRETDVEIIESLVDDVAKEYEEKTGKPIKIEL-SSSYLNKDIAGGV 184
P I++ R D+ ++E+ V EY + K +++++ +YL + AGGV
Sbjct: 122 QGLLRLLEPVMIVRCRPQDLLLVEAAVQKAIPEYMTISQKHVEVQIDKEAYLAVNAAGGV 181
Query: 185 IVSNGNGRIEVDNTXXXXXXXXXXXXXPAIRLELFGPSKTRKFF 228
V +GN RI+V NT P IR+ LFG + RKFF
Sbjct: 182 EVYSGNQRIKVSNTLESRLDLSAKQKMPEIRMALFGANTNRKFF 225
>7296718 [C] KOG1664 Vacuolar H+-ATPase V1 sector subunit E
Length = 226
Score = 93.6 bits (231), Expect = 2e-19
Identities = 57/225 (25%), Positives = 103/225 (45%), Gaps = 1/225 (0%)
Query: 6 ALSPNQVNDELQKMQXXXXXXXXXXXXXXXXXXQQEYEIEKTGLVRNETSAIDNNIASRM 65
ALS V +++ M ++E+ IEK LV+ + I +
Sbjct: 2 ALSDADVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQQQRLKIMEYYEKKE 61
Query: 66 KKAALKQQIVKSTIANKMRLKVLGTREEVLDSIFEKTKAELKQISSKKEEYKPXXXXXXX 125
K+ L+++I S + N+ RLKVL RE+ + S+ + + L +++ + EY+
Sbjct: 62 KQVELQKKIQSSNMLNQARLKVLKVREDHVSSVLDDARKRLGEVTKNQSEYETVLTKLIV 121
Query: 126 XXXXXXXXPSAIIKVRETDVEIIESLVDDVAKEYEEKTGKPIKIEL-SSSYLNKDIAGGV 184
P I++ RE DV ++ +++ ++Y+ + + +++ + +L+ D GGV
Sbjct: 122 QGLFQIMEPKVILRCREVDVPLVRNVLPAAVEQYKAQINQNVELFIDEKDFLSADTCGGV 181
Query: 185 IVSNGNGRIEVDNTXXXXXXXXXXXXXPAIRLELFGPSKTRKFFD 229
+ NGRI+V NT P IR LFG + RKF D
Sbjct: 182 ELLALNGRIKVPNTLESRLDLISQQLVPEIRNALFGRNVNRKFTD 226
>Hs4502317 [C] KOG1664 Vacuolar H+-ATPase V1 sector subunit E
Length = 226
Score = 90.5 bits (223), Expect = 2e-18
Identities = 56/225 (24%), Positives = 99/225 (43%), Gaps = 1/225 (0%)
Query: 6 ALSPNQVNDELQKMQXXXXXXXXXXXXXXXXXXQQEYEIEKTGLVRNETSAIDNNIASRM 65
ALS V +++ M ++E+ IEK LV+ + I +
Sbjct: 2 ALSDADVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKKE 61
Query: 66 KKAALKQQIVKSTIANKMRLKVLGTREEVLDSIFEKTKAELKQISSKKEEYKPXXXXXXX 125
K+ +++I S + N+ RLKVL R++++ + + K L ++ Y+
Sbjct: 62 KQIEQQKKIQMSNLMNQARLKVLRARDDLITDLLNEAKQRLSKVVKDTTRYQVLLDGLVL 121
Query: 126 XXXXXXXXPSAIIKVRETDVEIIESLVDDVAKEYEEKTGKPIKIELSS-SYLNKDIAGGV 184
P I++ R+ D ++++ V Y+ T + +++ SYL +DIAGGV
Sbjct: 122 QGLYQLLEPRMIVRCRKQDFPLVKAAVQKAIPMYKIATKNDVDVQIDQESYLPEDIAGGV 181
Query: 185 IVSNGNGRIEVDNTXXXXXXXXXXXXXPAIRLELFGPSKTRKFFD 229
+ NG+ +I+V NT P +R LFG + RKF D
Sbjct: 182 EIYNGDRKIKVSNTLESRLDLIAQQMMPEVRGALFGANANRKFLD 226
>CE19362 [C] KOG1664 Vacuolar H+-ATPase V1 sector subunit E
Length = 226
Score = 87.4 bits (215), Expect = 1e-17
Identities = 54/224 (24%), Positives = 98/224 (43%), Gaps = 1/224 (0%)
Query: 7 LSPNQVNDELQKMQXXXXXXXXXXXXXXXXXXQQEYEIEKTGLVRNETSAIDNNIASRMK 66
+S N V +L+ M ++E+ IEK LV+ + I + K
Sbjct: 3 ISDNDVQKQLRHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQQQRQKIMEFFEKKEK 62
Query: 67 KAALKQQIVKSTIANKMRLKVLGTREEVLDSIFEKTKAELKQISSKKEEYKPXXXXXXXX 126
+ L+++I S N RL+ L RE+ + ++ ++ ++ L +IS Y
Sbjct: 63 QVELQRKIQASNSLNAGRLRCLKAREDHIGAVLDEARSNLSRISGDAARYPAILKGLVMQ 122
Query: 127 XXXXXXXPSAIIKVRETDVEIIESLVDDVAKEYEEKTGKPIKIEL-SSSYLNKDIAGGVI 185
+++ RE D+ ++E L+ + +++ G K+ L ++L + AGGV
Sbjct: 123 GLLQLLEKEVVLRCREKDLRLVEQLLPECLDGLQKEWGSTTKVVLDKQNFLPSESAGGVE 182
Query: 186 VSNGNGRIEVDNTXXXXXXXXXXXXXPAIRLELFGPSKTRKFFD 229
+S G+I+V +T P +R LFGP+ R FFD
Sbjct: 183 LSARAGKIKVSSTLESRLELIANQIVPQVRTALFGPNPNRSFFD 226
>At3g08560 [C] KOG1664 Vacuolar H+-ATPase V1 sector subunit E
Length = 235
Score = 77.4 bits (189), Expect = 2e-14
Identities = 49/230 (21%), Positives = 103/230 (44%), Gaps = 16/230 (6%)
Query: 12 VNDELQKMQXXXXXXXXXXXXXXXXXXQQEYEIEKTGLVRNETSAIDNNIASRMKKAALK 71
V+ ++Q+M ++E+ IE+ L+ + + + ++K+ ++
Sbjct: 6 VSKQIQQMVRFIRQEAEEKANEISISAEEEFNIERLQLLESAKRKLRQDYDRKLKQVDIR 65
Query: 72 QQIVKSTIANKMRLKVLGTREEVLDSIFEKTKAELKQISSKKEEYKPXXXXXXXXXXXXX 131
++I ST N R+K L +++V+ ++ + +L ++S+ K YK
Sbjct: 66 KRIDYSTQLNASRIKYLQAQDDVVTAMKDSAAKDLLRVSNDKNNYKKLLKSLIIESLLRL 125
Query: 132 XXPSAIIKVRETDVEIIESLVDDVAKEYEE--KTGKPIKIELSSSYL------------- 176
PS +++ RE D +++ES+++D ++Y E K G P KI +
Sbjct: 126 KEPSVLLRCREMDKKVVESVIEDAKRQYAEKAKVGSP-KITIDEKVFLPPPPNPKLPDSH 184
Query: 177 NKDIAGGVIVSNGNGRIEVDNTXXXXXXXXXXXXXPAIRLELFGPSKTRK 226
+ +GGV++++ +G+I +NT P IR L G +T +
Sbjct: 185 DPHCSGGVVLASQDGKIVCENTLDARLDVAFRQKLPQIRTRLVGAPETSR 234
>At4g11150 [C] KOG1664 Vacuolar H+-ATPase V1 sector subunit E
Length = 230
Score = 75.9 bits (185), Expect = 4e-14
Identities = 52/226 (23%), Positives = 101/226 (44%), Gaps = 12/226 (5%)
Query: 7 LSPNQVNDELQKMQXXXXXXXXXXXXXXXXXXQQEYEIEKTGLVRNETSAIDNNIASRMK 66
++ V+ ++Q+M ++E+ IEK LV E I + + K
Sbjct: 1 MNDGDVSRQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQDYEKKEK 60
Query: 67 KAALKQQIVKSTIANKMRLKVLGTREEVLDSIFEKTKAELKQISSKKEEYKPXXXXXXXX 126
+A ++++I S N R+KVL ++++++++ ++ +L +S + YK
Sbjct: 61 QADVRKKIDYSMQLNASRIKVLQAQDDIVNAMKDQAAKDLLNVSRDEYAYKQLLKDLIVQ 120
Query: 127 XXXXXXXPSAIIKVRETDVEIIESLVDDVAKEYEEK-------TGKPIKIELSSSYLNKD 179
PS +++ RE D+ ++E+++DD +EY K KI L + D
Sbjct: 121 CLLRLKEPSVLLRCREEDLGLVEAVLDDAKEEYAGKAKVHAPEVAVDTKIFLPPPPKSND 180
Query: 180 -----IAGGVIVSNGNGRIEVDNTXXXXXXXXXXXXXPAIRLELFG 220
+GGV++++ +G+I +NT P IR LFG
Sbjct: 181 PHGLHCSGGVVLASRDGKIVCENTLDARLDVAFRMKLPVIRKSLFG 226
>At1g64200 [C] KOG1664 Vacuolar H+-ATPase V1 sector subunit E
Length = 237
Score = 75.5 bits (184), Expect = 6e-14
Identities = 51/204 (25%), Positives = 96/204 (47%), Gaps = 26/204 (12%)
Query: 39 QQEYEIEKTGLVRNETSAIDNNIASRMKKAALKQQIVKSTIANKMRLKVLGTREEVLDSI 98
++E+ IEK LV E I + K+ ++++I S N R+KVL ++++++++
Sbjct: 33 EEEFNIEKLQLVEAEKKKIRQEYEKKEKQVDVRKKIDYSMQLNASRIKVLQAQDDIVNAM 92
Query: 99 FEKTKAELKQISSK------KEEYKPXXXXXXXXXXXXXXXPSAIIKVRETDVEIIESLV 152
E+ +L ++S +YK P+ +++ RE D++I+ES++
Sbjct: 93 KEEAAKQLLKVSQHGFFNHHHHQYKHLLKDLIVQCLLRLKEPAVLLRCREEDLDIVESML 152
Query: 153 DDVAKEYEEKTGKPIKIELSSSYLNKDI----------------AGGVIVSNGNGRIEVD 196
DD ++EY +K K+ ++KDI AGGV++++ +G+I +
Sbjct: 153 DDASEEYCKKA----KVHAPEIIVDKDIFLPPAPSDDDPHALSCAGGVVLASRDGKIVCE 208
Query: 197 NTXXXXXXXXXXXXXPAIRLELFG 220
NT P IR LFG
Sbjct: 209 NTLDARLEVAFRNKLPEIRKSLFG 232
>Hs18549288 [C] KOG1664 Vacuolar H+-ATPase V1 sector subunit E
Length = 151
Score = 62.0 bits (149), Expect = 7e-10
Identities = 37/150 (24%), Positives = 66/150 (43%), Gaps = 1/150 (0%)
Query: 81 NKMRLKVLGTREEVLDSIFEKTKAELKQISSKKEEYKPXXXXXXXXXXXXXXXPSAIIKV 140
N+ RLKVL R++++ + + K L ++ Y+ I++
Sbjct: 2 NQARLKVLRARDDLITDLLNEAKQRLSKVVKDTTRYQVLLDGLVLQGLYQLLEHRMIVRC 61
Query: 141 RETDVEIIESLVDDVAKEYEEKTGKPIKIELSS-SYLNKDIAGGVIVSNGNGRIEVDNTX 199
R+ D+ ++++ V Y+ T + +++ SYL +DIAGGV + NGN +I+V T
Sbjct: 62 RKQDLPLVKAAVQKAIPMYKIATKNNVDVQIDQESYLPEDIAGGVEIYNGNHKIKVSKTP 121
Query: 200 XXXXXXXXXXXXPAIRLELFGPSKTRKFFD 229
++ FG RKF D
Sbjct: 122 ESQLDLIAQQMMLEVQGAFFGAKANRKFLD 151
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.309 0.128 0.327
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,376,162
Number of Sequences: 60738
Number of extensions: 302528
Number of successful extensions: 843
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 827
Number of HSP's gapped (non-prelim): 10
length of query: 229
length of database: 30,389,216
effective HSP length: 103
effective length of query: 126
effective length of database: 24,133,202
effective search space: 3040783452
effective search space used: 3040783452
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)