ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactVI0784   suspect: LH   R     KOG2820  General function prediction only                               FAD-dependent oxidoreductase

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactVI0784  267100  268398 433  
         (433 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC139.04c [R] KOG2820 FAD-dependent oxidoreductase                  226   7e-59
SPBC354.15 [R] KOG2820 FAD-dependent oxidoreductase                   137   4e-32

>SPAC139.04c [R] KOG2820 FAD-dependent oxidoreductase
          Length = 433

 Score =  226 bits (575), Expect = 7e-59
 Identities = 150/434 (34%), Positives = 224/434 (51%), Gaps = 51/434 (11%)

Query: 6   VVIVGAGIFGLSTAVQLARSKY--KVVVVDKFQIPSQLSASCDYNKIVRLEYNDEVYAAL 63
           +VIVG G+FGLSTAV+LA++     ++ +D   +PS +SA+ D NKIVR EY D  Y  L
Sbjct: 5   IVIVGCGVFGLSTAVELAKNHSFDNIIAIDAEPVPSSMSAANDINKIVRPEYADLKYMKL 64

Query: 64  AVEAMGYWHGESDKFLPRGMLHDCYSHCGRLSAIQDKNTSRYAFDNDSLQLLQTKFNKCK 123
           A+EAM  W  + +       L   Y  CGRLS I  K+  R  FD  + + L+       
Sbjct: 65  ALEAMEKWRNDPE-------LSSVYFECGRLSTIS-KDPYRARFDEVAQRNLRKLLGDSA 116

Query: 124 TVELHKELTCGGKFPQ-FSHSQRYDEIR--YNPDCGIGLARDSLVTMKNYAESLGVVF-- 178
            + L        K+P  FS+S    +++   N   G   +  SL  ++  A  LGV F  
Sbjct: 117 LINLSSSEEIRKKYPSLFSNSPLRSDMQAVVNEHAGYANSAASLKLLELKARELGVEFVF 176

Query: 179 ------------------HENDGAVSVSAGTVQCESGRKFLGHKIIVACGANTVSLLPMY 220
                              +ND  VSV     Q E G  +    I++A GA   + L   
Sbjct: 177 GKAGKFKKFVVNHSETDIDKNDNHVSV-----QTEDGTIYHADTILLAVGAYLNAYLNTS 231

Query: 221 GQIRATGLYVGHIQLTDKEYQKLKDIPVVFNSSLGYLFPPDKHTRILKICTSAM----SA 276
            ++ A GL V HIQLTD+E++  K++P++F+    Y FPP   T+++K+ ++      + 
Sbjct: 232 HRVCAKGLPVAHIQLTDEEFKTYKNMPIIFDPDCAYAFPPYPVTKLIKLASTGYEYVCNV 291

Query: 277 YDDMSNKSRAVYRALQPDAAPSVPIEAVVRIRTVLGKYLPDLVFDTKRKSKLRDIIDCKI 336
             D    S+ V       +  S+P  A++++R  L  +LPDL          R +I+ K+
Sbjct: 292 ETDYDENSKVVSIPHSGPSKSSLPKYAIIQMRRFLDTFLPDLAD--------RSLINTKM 343

Query: 337 CWISDTSNSDFIIDMIPDLKNVYVCCGDSGHAYKFLPNIGSYVQQKIEGRLAPILAHKWR 396
           CWISDT +++F+ID +P   NV+V  GDSGHA+KFLPNIG Y+ Q+I G L+      WR
Sbjct: 344 CWISDTEDANFLIDKVPQFDNVFVANGDSGHAFKFLPNIGRYIAQRILGDLSEEWKDAWR 403

Query: 397 YRESDWNGTEIEWR 410
           +RE D   +E++WR
Sbjct: 404 WREDD-KASELKWR 416


>SPBC354.15 [R] KOG2820 FAD-dependent oxidoreductase
          Length = 412

 Score =  137 bits (344), Expect = 4e-32
 Identities = 115/416 (27%), Positives = 193/416 (45%), Gaps = 39/416 (9%)

Query: 1   MSNYDVVIVGAGIFGLSTAVQLA-RSKYKVVVVDKFQIPSQLSASCDYNKIVRLEYNDEV 59
           + N  V+IVGAG+FGLS A++L  R  Y + ++D+   P    +S D N+I+R +Y D V
Sbjct: 2   VKNTSVIIVGAGVFGLSAALELTKRGGYTIKILDRAPPPVIDGSSVDANRIIRSDYADAV 61

Query: 60  YAALAVEAMGYWHGE---SDKFLPRGMLHDCYSHCGRLSAIQDKNTSRYAFDNDSLQLLQ 116
           Y ++ ++A+  W       ++F   G++            +   N        ++L  + 
Sbjct: 62  YCSMGIDALEEWRTNPLFKEQFYGSGLMF-----------VGRDNVEYRDMSLENLTKMG 110

Query: 117 TKFNKCKTVELHKELTCGGKFPQFSHSQRYDEIRY-NPDCGIGLARDSLVTMKNYAESLG 175
               K +T E  ++L     FP++       E  Y N   G   A  S+ ++ NY    G
Sbjct: 111 VSAAKFQTTEELRKL-----FPKWIGELNDGEAGYANFSSGWANAEQSVKSVVNYLAHAG 165

Query: 176 VVFHEN-DGAVSVSAGTVQCESGRK-----FLGHKIIVACGANTVSLLPM-YGQIRATGL 228
           V F    +G V           G +     ++  K+I A GA T SLLP  + +  ATG 
Sbjct: 166 VSFISGPEGTVEELITEENVVKGVRTTTGAYMAEKLIFATGAWTASLLPNDHTRFLATGQ 225

Query: 229 YVGHIQLTDKEYQKLKDIPVVFNSSLG-YLFPP--DKHTRILKICTSAMSAYDDMSNKSR 285
            V +I+LT +EY +    PV  +   G Y+FPP  D + +  +         +  S K  
Sbjct: 226 PVAYIKLTPEEYIRFLTNPVYLDFDTGFYIFPPTPDGYLKFARHGYGFTRMQNLKSGKVE 285

Query: 286 AVYRALQPDAAPSVPIEAVVRIRTVLGKYLPDLVFDTKRKSKLRDIIDCKICWISDTSNS 345
           +V    +P  +P +P EA + +R  L +   + +         R     +IC+ +DT+++
Sbjct: 286 SV-PPKKPLVSPILPKEAELDLRRNLQRTYGEEISQ-------RPFYKTRICYYTDTADA 337

Query: 346 DFIIDMIPDLKNVYVCCGDSGHAYKFLPNIGSYVQQKIEGRLAPILAHKWRYRESD 401
           +F+ D  PD +N++VC G SGH +KF P +G Y    +   L   L  KWR+++ +
Sbjct: 338 EFVFDYHPDYENLFVCTGGSGHGFKFFPILGKYSIGCMFRELEEPLLKKWRWKKEN 393


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.320    0.137    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 27,041,662
Number of Sequences: 60738
Number of extensions: 1175321
Number of successful extensions: 2622
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2612
Number of HSP's gapped (non-prelim): 2
length of query: 433
length of database: 30,389,216
effective HSP length: 109
effective length of query: 324
effective length of database: 23,768,774
effective search space: 7701082776
effective search space used: 7701082776
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)