ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI0926 good J KOG1416 Translation, ribosomal structure and biogenesis tRNA(1-methyladenosine) methyltransferase, subunit GCD10
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI0926 318615 317188 -476
(476 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YNL062c [J] KOG1416 tRNA(1-methyladenosine) methyltransferase su... 540 e-153
SPBC800.08 [J] KOG1416 tRNA(1-methyladenosine) methyltransferase... 164 4e-40
Hs19923475 [J] KOG1416 tRNA(1-methyladenosine) methyltransferase... 118 2e-26
At2g45730 [J] KOG1416 tRNA(1-methyladenosine) methyltransferase ... 108 2e-23
7297137 [J] KOG1416 tRNA(1-methyladenosine) methyltransferase su... 85 2e-16
CE15433 [J] KOG1416 tRNA(1-methyladenosine) methyltransferase su... 73 1e-12
>YNL062c [J] KOG1416 tRNA(1-methyladenosine) methyltransferase subunit GCD10
Length = 478
Score = 540 bits (1390), Expect = e-153
Identities = 271/467 (58%), Positives = 341/467 (72%), Gaps = 29/467 (6%)
Query: 7 IDPLTQISPNQHVLIRLPSDNLKIVELKANAIVSLGKFGAFRVNEIIGYRFGTTFDIVYD 66
++ LT I NQHV++RLPS N KIVELK N VSLGKFGAF VN+IIGY FG TF+I YD
Sbjct: 1 MNALTTIDFNQHVIVRLPSKNYKIVELKPNTSVSLGKFGAFEVNDIIGYPFGLTFEIYYD 60
Query: 67 GVEEEFVKGTQTM------IGKISVLENRLK---------ASSPALE------------- 98
G E + + IGK+ +L +K S P L
Sbjct: 61 GEEVSSDENRDSKPKNKIPIGKVRLLSQEIKDVNNDKDDGQSEPPLSIKEKSVSLELSSI 120
Query: 99 NSSENNRGLINLGSVVQEMSMAEIEAMKREAASGDAIISKMIESHKSFHQKTVHSQEKYL 158
+SS N+ L+N+GS QE+++ EIE MK+E+ S II K+I+SHKSFH KTV+SQEKY+
Sbjct: 121 DSSATNQNLVNMGSKAQELTVEEIEKMKQESLSSKEIIDKIIKSHKSFHNKTVYSQEKYV 180
Query: 159 KRKKQKFAKFFTVEYLDSSGLLHYLIEKGDVLRVMDISQESLGMALNLANINSNGQYLCI 218
RKKQKFAK+FTVEYL SS LL +LI+KGD+ RV+D+SQES+GM LNLANI S G YLC+
Sbjct: 181 NRKKQKFAKYFTVEYLSSSNLLQFLIDKGDIQRVLDMSQESMGMLLNLANIQSEGNYLCM 240
Query: 219 DETGGLIVYAMLERMFAGDSNSKANGKIVVVHENEHPNLDLLKFSSYSDNFIQRHVKTIS 278
DETGGL+VY +LERMF GD+ SK+ GK++V+HENEH NLDLLKF++YS+ FI+ HV TIS
Sbjct: 241 DETGGLLVYFLLERMFGGDNESKSKGKVIVIHENEHANLDLLKFANYSEKFIKEHVHTIS 300
Query: 279 VLDFFEPAKEADIKSLFKPLSAEEINDLKSNKKSAYFRRLKWYHNQLSNIEVAAS-SFDG 337
+LDFFEP +I+S F PL EE LK KK++Y+R+L+WY+ Q +E+ +DG
Sbjct: 301 LLDFFEPPTLQEIQSRFTPLPKEEARALKGGKKNSYYRKLRWYNTQWQILELTGEFLYDG 360
Query: 338 LLVASTLYLPTLIPRLGEKIHGSRPIVCYSQYKEPLLELSHSLYENLNYLAPSLLETRCR 397
L++A+TL+LPTL+P+L EKIHGSRPIVCY Q+KE LLEL+H+LY +L +LAPS+LETRCR
Sbjct: 361 LVMATTLHLPTLVPKLAEKIHGSRPIVCYGQFKETLLELAHTLYSDLRFLAPSILETRCR 420
Query: 398 PYQTVRGKLHPLMTMKGGGGYLMWCHRVLPAAEPKLHQETLEKSEIK 444
PYQ++RGKLHPLMTMKGGGGYLMWCHRV+PA EP T S K
Sbjct: 421 PYQSIRGKLHPLMTMKGGGGYLMWCHRVIPAPEPVSENATAADSSEK 467
>SPBC800.08 [J] KOG1416 tRNA(1-methyladenosine) methyltransferase subunit GCD10
Length = 462
Score = 164 bits (414), Expect = 4e-40
Identities = 131/457 (28%), Positives = 222/457 (47%), Gaps = 75/457 (16%)
Query: 13 ISPNQHVLIRLPSDNLKIVELKANAIVSLGKFGAFRVNEIIGYRFGTTFDIVYDGVEEEF 72
IS N V I+LPSDN++ V LK N + LGKFG+F +++ G F TF+I
Sbjct: 8 ISENSSVFIKLPSDNVRFVTLKPNNTIHLGKFGSFLADDLFGKHFDETFEIYQP------ 61
Query: 73 VKGTQTMIGKISVLENRLKASSPALENSSENNRGLINLGSVVQEMSMAEIEAMKREAASG 132
KI VL+ R +E + N+ L N Q M+ EI+ ++ +G
Sbjct: 62 --------KKIRVLKTR---EVQYIEEEKKTNQEL-NDCRGNQLMTQEEIDELRANIKAG 109
Query: 133 ----DAIISKMIESHKSFHQKTVHSQEKYLKRKKQKFAKFFTVEYLDSSGLLHYLIEKGD 188
+ I ++ S K+F QKT+ +QEKY+ RK +K+ + F V + +Y+IE D
Sbjct: 110 GLRAEEAIKQLTNSSKTFEQKTLFAQEKYVTRKGEKYLQRFQVLRPCVEVVANYMIEH-D 168
Query: 189 VLRVMDISQESLGMALNLANINSNGQYLCIDETGGLIVYAMLERMFAGDSNSKANGKIVV 248
+++D++ E + + L L N+ G+YL +DETG + + ++++R+ AGD KI +
Sbjct: 169 PYKILDLTAECISLMLTLGNVKPGGRYLVVDETGCMFLGSLIDRV-AGDC------KITL 221
Query: 249 VHENEHPNLDLLKF---SSYSDNFIQRHV-KTISVLDFFEPAKEADIKSLFKPLSAEEIN 304
VH NE PN L++ D+ +Q+ + KT++ P + S+ + + E+N
Sbjct: 222 VHPNEQPNSSCLEYWGQDFKEDSLVQKGILKTLNWYQVTNPTETLSEYSV-EDIPESELN 280
Query: 305 DLKSNKKSAY------FRRLKWYHNQLSNIEVAASSFDGLLVASTLYLPTLIPRLGEKIH 358
++K + Y F RLK N + + E + ++D L + S +++ L K+
Sbjct: 281 EMKLRHRKRYETKKATFNRLK---NTIDDFE--SGNYDALFILSIHTPMSVLQHLLPKLG 335
Query: 359 GSRPIVCYSQYKEPLLELSHSL--YENL---------------------------NYLAP 389
SRP + YS Y++ L+E H L ++NL +
Sbjct: 336 ISRPFMVYSTYQQVLVETYHQLSKWDNLFVEKTAQSTENDEKVDQGDVAIDTQKEKVIML 395
Query: 390 SLLETRCRPYQTVRGKLHPLMTMKGGGGYLMWCHRVL 426
+ E R RPYQ + + HP MT++G G+++ +VL
Sbjct: 396 DIHEIRTRPYQVLPERTHPFMTVRGDMGFVLSGIKVL 432
>Hs19923475 [J] KOG1416 tRNA(1-methyladenosine) methyltransferase subunit GCD10
Length = 497
Score = 118 bits (296), Expect = 2e-26
Identities = 121/499 (24%), Positives = 216/499 (43%), Gaps = 84/499 (16%)
Query: 26 DNLKIVELKANAIVSLGKFGAFRVNEIIGYRFGTTFDIVYDGVEEEFVKGTQTMIGKISV 85
D K V+++ V+ K F ++ +IG+ +GT F++ G
Sbjct: 32 DVFKAVQVQRRKKVTFEKQW-FYLDNVIGHSYGTAFEVTSGGS----------------- 73
Query: 86 LENRLKASSPALEN--SSENNRGLINLGSVVQEMSMAEIEAMKREAASGDAIISKMIESH 143
L+ + K P E + +NR +++ G Q+++ +I+A+K + G+ I+ ++IE+
Sbjct: 74 LQPKKKREEPTAETKEAGTDNRNIVDDGKS-QKLTQDDIKALKDKGIKGEEIVQQLIENS 132
Query: 144 KSFHQKTVHSQEKYLKRKKQKFAKFFTVEYLDSS--GLLHYLIEKGDVLRVMDISQESLG 201
+F KT +Q+KY+K+KK+K+ TV + +++Y E G ++ + ++L
Sbjct: 133 TTFRDKTEFAQDKYIKKKKKKYEAIITVVKPSTRILSIMYYAREPG---KINHMRYDTLA 189
Query: 202 MALNLANINSNGQYLCIDETGGLIVYAMLER---------MFAGDSNSKA--------NG 244
L L NI + + + ++ GL++ AM+ER ++ G +A
Sbjct: 190 QMLTLGNIRAGNKMIVMETCAGLVLGAMMERMGGFGSIIQLYPGGGPVRAATACFGFPKS 249
Query: 245 KIVVVHENEHPNLDLLKFSSYSDNFIQRHVKTISVLD----FFEPAKEADIKSLFKPLSA 300
+ ++E +D L ++S + K ++++ E + ++ ++ A
Sbjct: 250 FLSGLYEFPLNKVDSLLHGTFSAKMLSSEPKDSALVEESNGTLEEKQASEQENEDSMAEA 309
Query: 301 EEIN---------------------DLKSNKKSAYFRRLKWYHNQLSNIEVAA----SSF 335
E N + S K ++ + + ++E AA +
Sbjct: 310 PESNHPEDQETMETISQDPEHKGPKERGSKKDYIQEKQRRQEEQRKRHLEAAALLSERNA 369
Query: 336 DGLLVASTLYLPTLIPRLGEKIHGSRPIVCYSQYKEPLLELSHSLYENLNYLAPSLLETR 395
DGL+VAS + L+ L + + SRP V Y QYKEPLLE L E + L ET
Sbjct: 370 DGLIVASRFHPTPLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGVINLRLSETW 429
Query: 396 CRPYQTVRGKLHPLMTMKGGGGYLMWCHRVLPAAEPKLHQETLEKSEIKN-SDEEGDEDA 454
R YQ + + HP + M GGGGYL+ V A L +T KS E +E A
Sbjct: 430 LRNYQVLPDRSHPKLLMSGGGGYLLSGFTV---AMDNLKADTSLKSNASTLESHETEEPA 486
Query: 455 HKLDQEKQVIKRRKPNEDN 473
K KR+ P D+
Sbjct: 487 AK--------KRKCPESDS 497
>At2g45730 [J] KOG1416 tRNA(1-methyladenosine) methyltransferase subunit GCD10
Length = 446
Score = 108 bits (269), Expect = 2e-23
Identities = 104/431 (24%), Positives = 187/431 (43%), Gaps = 66/431 (15%)
Query: 26 DNLKIVELKANAIVSLGKFGAFRVNEIIGYRFGTTFDIVYDGVEEEFVKGTQTMIGKISV 85
D L L + AI+ +G + + +IG FG+ F V G + F+ +
Sbjct: 38 DRLVFARLSSGAILKIGNKN-YSLKPLIGAPFGSLFQ-VETGEDGSFLS---------RI 86
Query: 86 LENRLKASSPALENSSENNRGLINLGSVVQEMSMAEIEAMKREAASGDAIISKMIESHKS 145
L + +++S + + S +NR +++ + Q ++ EIEAM+RE A GD II +I + K+
Sbjct: 87 LPIKQESNSNNVMDDSRDNREIVD-NNEAQNLTGEEIEAMRREGAKGDEIIEALIANSKT 145
Query: 146 FHQKTVHSQEKYLKRKKQKFAKFFTVEYLDSSGLLHYLIEKGDVLRVMDISQESLGMALN 205
F +K SQEKY +K++K+A + + + +K R+ + ++L + L
Sbjct: 146 FDKKFQLSQEKYKLKKQKKYAPKLLLRRPFARSICEAYFKKYPA-RIGFLRVDALSLLLT 204
Query: 206 LANINSNGQYLCIDETGGLIVYAMLERMFAGDSNSKANGKIVVVHENEHP-NLDLLKFSS 264
+AN+ + L +D GGL+ A+ ER+ G + ++ P +++++ +
Sbjct: 205 MANVTAYSDVLVVDMVGGLVTGAVAERL-------GGTGYVCNTYKGSSPYPVEMVRMFN 257
Query: 265 YSDNFIQRHV----------KTIS-------------------------VLDFFEPAKEA 289
+SD ++R V KT S +++ EA
Sbjct: 258 FSDKVLKRIVQSSINELSSAKTASTEGNNQQEGVCKAENNINETATSDVMVEEISVTTEA 317
Query: 290 DIKSLFKPLSAEEINDLKSNKKSAYFRRLKWYHNQLSNIEVAASSFDGLLVASTLYLPTL 349
+ + P ++ I K+ K++ W N S++ +AA D +VA + LP L
Sbjct: 318 GVVDIVVP-ESKIIKAAKAGAKASKETIEMWQENGFSSLIMAAQDQDPWIVAKDV-LPLL 375
Query: 350 IPRLGEKIHGSRPIVCYSQYKEPLLELSHSLYENLNYLAPSLLETRCRPYQTVRGKLHPL 409
S P Y QY +PL H L + + + E R YQ + + HP
Sbjct: 376 --------SYSAPFAIYHQYLQPLATCMHKLQQGKMAINLQITEPWLREYQVLPSRSHPH 427
Query: 410 MTMKGGGGYLM 420
M M GGY++
Sbjct: 428 MQMSSFGGYVL 438
>7297137 [J] KOG1416 tRNA(1-methyladenosine) methyltransferase subunit GCD10
Length = 464
Score = 85.1 bits (209), Expect = 2e-16
Identities = 89/372 (23%), Positives = 149/372 (39%), Gaps = 55/372 (14%)
Query: 97 LENSSENNRGLINLGSVVQEMSMAEIEAMKREAASGDAIISKMIESHKSFHQKTVHSQEK 156
+ +S +NR + + G Q + +I ++ E II K++E+ K+FH +T +SQEK
Sbjct: 94 ISSSGADNRDIRDDGEA-QTLKPEDIAQLREECNDSSKIIEKLVENSKTFHNRTEYSQEK 152
Query: 157 YLKRKKQKFAKFFTVEYLDSSGLLHYLIEKGDVLRVMDISQESLGMALNLANINSNGQYL 216
YL +K++K+ +F + + L+ + + D +VM I ++L ++ + + G YL
Sbjct: 153 YLLKKEKKYFEFVQIRQ-PTIRLMLDIFYRQDSEKVMGIRVDTLSQIISYSGVCGFGSYL 211
Query: 217 CIDE-TGGLIVYAMLERMFAGDSNSKANGKIVVVHENEHPNLDLLKFSSYSDNFIQR--H 273
+ T GL+ AML + AG G +V +H P L QR
Sbjct: 212 LYESGTNGLLPAAMLNSIGAG-----TEGTLVHMHPGNVPQKQALSALKLPLEQQQRCVS 266
Query: 274 VKTISVL-DFFE--PAKEADI----------------------KSLFKPLSAEEINDLKS 308
V SVL +F++ AK DI +P + + D +
Sbjct: 267 VNLYSVLREFYQGGDAKATDIPVVEPSENEPQETQLETPTEEQPEAIEPTTKKPKLDESN 326
Query: 309 NKKSAYFRRLKWYHNQLSNIEVAA--------------------SSFDGLLVASTLYLPT 348
+ + K + I V + FD L+VA+ +
Sbjct: 327 SGREGIGHNSKLIKTSTNTISVVIGAKGPPRWHLENKRATALMHAEFDSLVVAAKEHPSN 386
Query: 349 LIPRLGEKIHGSRPIVCYSQYKEPLLELSHSLYENLNYLAPSLLETRCRPYQTVRGKLHP 408
++ L + SRP+V +S KE L E L L R YQ + + HP
Sbjct: 387 ILQALLPLVKPSRPVVVFSTCKELLQETFMELKTTGKVTGLHLTSNWLRTYQILPNRTHP 446
Query: 409 LMTMKGGGGYLM 420
+ M G GYL+
Sbjct: 447 EVNMSGNSGYLL 458
>CE15433 [J] KOG1416 tRNA(1-methyladenosine) methyltransferase subunit GCD10
Length = 473
Score = 72.8 bits (177), Expect = 1e-12
Identities = 80/370 (21%), Positives = 152/370 (40%), Gaps = 55/370 (14%)
Query: 86 LENRLKASSPALENSSENNRGLINLGSVVQEMSMAEIEAMKREAASGDAIISKMIESHKS 145
L+ R+ + AL+ E R Q++ M + MK++ SG +++K++E S
Sbjct: 103 LDPRVVIAPSALKLEPEQKR---------QKLEMDAVLEMKKQGVSGQEMVAKLVEGSAS 153
Query: 146 FHQKTVHSQEKYLKRKKQKFA-KFFTVEYLDSSGLLHYLIEKGDVLRVM----------- 193
F +TV SQ KY+KRK +K + ++F ++D SG+L + RV+
Sbjct: 154 FQTRTVFSQSKYIKRKAKKHSDRYF---FIDKSGVLPISTSANNNFRVLILRPTIRLLAK 210
Query: 194 -----------DISQESLGMALNLANINSNGQYLCIDETGGLIVYAMLERMFAGDSNSKA 242
+ + L + L +A ++ + ++T GLI A++ER+
Sbjct: 211 AYYLKDPDRLAMLRADQLALILQMAGLHHGKNVVVFEQTLGLITSAVIERL-------GG 263
Query: 243 NGKIVVVHENE-HPNLDLLKFSSYSDN----FIQRHVKTISV-----LDFFEPAKEADIK 292
G + +H ++ + +Y DN F+ +K + D A D
Sbjct: 264 TGACIHIHRGAVAQSIPCVHSMNYDDNTLSTFLPVRIKCVLAGKHLPYDNNRKAVNGDDD 323
Query: 293 SLFKPLSAEEINDLKSNKKSAYFRRLKWYHNQLSNIE-VAASSFDGLLVASTLYLP-TLI 350
+L + +E ++ A RR + I+ + L++ S P +++
Sbjct: 324 NLEEVNGDKEPEKIEDQDLEALARRNDRLDREKRGIDLINDEQIHSLIIGSRTVDPISVL 383
Query: 351 PRLGEKIHGSRPIVCYSQYKEPLLELSHSLYENLNYLAPSLLETRCRPYQTVRGKLHPLM 410
L K+ S IV YS ++ L+ ++ + L + CR Q + + HPLM
Sbjct: 384 ELLYPKLAPSGTIVIYSPHQNILIS-AYDWLSKRQTINLVLTDQMCRVMQVLPDRTHPLM 442
Query: 411 TMKGGGGYLM 420
GG+++
Sbjct: 443 AQHVAGGHIL 452
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.134 0.374
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,461,309
Number of Sequences: 60738
Number of extensions: 1264014
Number of successful extensions: 5172
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 5153
Number of HSP's gapped (non-prelim): 10
length of query: 476
length of database: 30,389,216
effective HSP length: 110
effective length of query: 366
effective length of database: 23,708,036
effective search space: 8677141176
effective search space used: 8677141176
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)