ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI1070 good S KOG3247 Function unknown Uncharacterized conserved protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI1070 374246 372822 -475
(475 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YIL104c [S] KOG3247 Uncharacterized conserved protein 539 e-153
SPBC8D2.13 [S] KOG3247 Uncharacterized conserved protein 216 8e-56
SPBPI052 [S] KOG3247 Uncharacterized conserved protein 187 2e-47
Hs8922500 [S] KOG3247 Uncharacterized conserved protein 152 1e-36
CE25308_2 [S] KOG3247 Uncharacterized conserved protein 92 2e-18
7298843 [S] KOG3247 Uncharacterized conserved protein 70 5e-12
Hs18575935 [S] KOG3247 Uncharacterized conserved protein 60 7e-09
>YIL104c [S] KOG3247 Uncharacterized conserved protein
Length = 507
Score = 539 bits (1389), Expect = e-153
Identities = 277/503 (55%), Positives = 355/503 (70%), Gaps = 37/503 (7%)
Query: 1 MITPRFTVTQDDEYIYVQIKVSSIRFNSAGVEVVIDGCMFIFHLSPYYLRLRLSDELVED 60
MITPRF++TQD+E+I+++I +S+IRF++ G+E++I M IFHLSPYYLRLR EL++D
Sbjct: 1 MITPRFSITQDEEFIFLKIFISNIRFSAVGLEIIIQENMIIFHLSPYYLRLRFPHELIDD 60
Query: 61 ETLNTVEYDSKQESINCKILKAVKGKEFXXXX------------XXXXXXXXXNEPKVTD 108
E +T +YDSK E IN K+ K K + F + K T
Sbjct: 61 ER-STAQYDSKDECINVKVAKLNKNEYFEDLDLPTKLLARQGDLAGADALTENTDAKKTQ 119
Query: 109 GPLIQEI------SNEKESIADIREEGEHFDWEIKQEVQVDNTP---TTKYGFDNLYTGY 159
PLIQE+ +N K+ + I + GE F+WEI+Q++ TKYGFDNLY
Sbjct: 120 KPLIQEVETDGVSNNIKDDVKTIGQMGEGFNWEIEQKMDSSTNNGILKTKYGFDNLYDTV 179
Query: 160 IVMSVANGNDINELDDPEHTSPENRVKERLKKENLKFDPEYYAAEYITIKQGDEEEIQIN 219
I +S +NGNDINELDDPEHT +RV ERL+KENLKFDPEYY +EY+T K G+EE+++IN
Sbjct: 180 ISVSTSNGNDINELDDPEHTDANDRVIERLRKENLKFDPEYYVSEYMTHKYGNEEDLEIN 239
Query: 220 GIKRLLGYLPPLCERYQQWFERSDNESKERNVSFPVEFTEAEQTQMQDHIPKKKYLINDV 279
GIK LL + P + ++Y QW++ S N N+ P+EFT+ EQ QMQD++PKK YL+ D+
Sbjct: 240 GIKELLKFTPSIVKQYLQWYKDSTNP----NLVMPIEFTDEEQKQMQDNLPKKSYLVEDI 295
Query: 280 KSQYLTILSILYSYTFEQIENEGQHTTESQWTIGKLTPQISMLDQQIL----LSESLIHF 335
K Y+TILS+L+SY FEQIENEG HTTES WT+GKL PQIS LDQQ+ L + +
Sbjct: 296 KPLYVTILSVLFSYVFEQIENEGTHTTESAWTMGKLCPQISFLDQQLKQVNELQDGMKEI 355
Query: 336 SQ-------IKGTIVTGIRRALSYPLHRNYDLCIKAWQLAIKLLLGGKRLVIKALLDIHE 388
S+ IK I+TGIRRALSYPLHRNYDL +KAW +L GGKRLVI+ALLDIHE
Sbjct: 356 SKVNKDSSLIKIAIITGIRRALSYPLHRNYDLAMKAWTFVYYILRGGKRLVIRALLDIHE 415
Query: 389 LFRYHDVYYVYNKILLDDLCAWFISQGNDNVIQSLALQAKKELESITKSDIEFECISGMN 448
FR+HDVYYVY+K+LLDDL AWFISQG++NVI+SLAL+ +KE ES++K DIEFECI+ N
Sbjct: 416 TFRFHDVYYVYDKVLLDDLTAWFISQGSENVIRSLALEMRKEQESLSKQDIEFECIASFN 475
Query: 449 EETGEVEWETLNISEIEYLAEQE 471
E+TGE EWETLNI E+E LAE E
Sbjct: 476 EQTGEPEWETLNIREMEILAESE 498
>SPBC8D2.13 [S] KOG3247 Uncharacterized conserved protein
Length = 451
Score = 216 bits (549), Expect = 8e-56
Identities = 148/485 (30%), Positives = 241/485 (49%), Gaps = 65/485 (13%)
Query: 1 MITPRFTVTQDDEYIYVQIKVSSIRFNSAGVEVVIDGCMFIFHLSPYYLRLRLSDELVED 60
MITP+F + QD+E++Y+ + I+ + ++ + F ++PY+L+L L +++D
Sbjct: 1 MITPKFELRQDEEFVYLDVHTPYIKAKNVEIDAFNNEVNFC--IAPYFLKLELPGNVLDD 58
Query: 61 ETLNTVEYDSKQESINCKILKAVKGKEFXXXXXXXXXXXXXNEPKVTDGPLIQEISN--- 117
+ N YD ++ K K G+ F N+ V PLI+E+ +
Sbjct: 59 DRANA-SYDISSGFVHIKFPKETPGEVFPDLDLLTTLLVNQNKEPVKK-PLIEEVEDTPS 116
Query: 118 -----------EKESIAD---IREEGEHFDWEIKQEV---QVDNTPTTKYGFDNLYTGYI 160
EKE D I + FDW + Q + ++ T T++YGF+N Y+G +
Sbjct: 117 PSTSQNASKDEEKEVKIDWQNIHFDDPDFDWGLLQRLDDEKMQYTSTSQYGFNNQYSGLL 176
Query: 161 VMSVANGNDINELDDPEHTSPENRVKERLKKENLKFDPEYYAAEYITIKQGDEEEIQING 220
+ GN+IN++ +PE T P R + R++ E+ KFD E+Y A++ + DE
Sbjct: 177 KYNALVGNEINQIPEPERTPPSTRSEIRVQLEDEKFDAEHYMADFYDREMIDE------- 229
Query: 221 IKRLLGYLPPLCERYQQWFERSDNESKERNVSFPVEFTEAEQTQMQDHIPKKKYLINDVK 280
+L Y+P Y F +R + F E EA +PK+ YLI++ K
Sbjct: 230 ---ILHYIPD----YIGEFLLVQQGKSDRLLEFTSEEKEALAK-----LPKRSYLIDNPK 277
Query: 281 SQYLTILSILYSYTFEQIENEGQHTTESQWTIGKLTPQISMLDQQILLSESLIHFSQIKG 340
+ YL ++ ++++Y ++ G T ES W IG L+ IS LD +F IK
Sbjct: 278 NVYLCLVPLIFAYAYDNRTTLGDPTIESCWNIGTLSSTISCLDT---------NFLAIKD 328
Query: 341 TIVTGIRRALSYPLHRNYDLCIKAWQLAIKLLLGGKRLVIKALLDIHELFRYHDVYYVYN 400
+ IRR+L+YPL+RN+DL + W+ +L GKR +++ LL I LF +HDVYY+YN
Sbjct: 329 IFIACIRRSLAYPLYRNWDLALTCWKDTHAILSLGKRWILRVLLRIRNLFEHHDVYYIYN 388
Query: 401 KILLDDLCAWFISQGNDNVIQSLALQAKKELESITKSDIEF----------ECISGMNEE 450
I+L+D W I NDNV+ +LA Q + +ITK + + E I +EE
Sbjct: 389 AIVLNDYAYW-IQTANDNVLAALAYQVSE--CTITKEETNWPLEEIEANVQETIIASDEE 445
Query: 451 TGEVE 455
+ +E
Sbjct: 446 SESIE 450
>SPBPI052 [S] KOG3247 Uncharacterized conserved protein
Length = 405
Score = 187 bits (476), Expect = 2e-47
Identities = 134/437 (30%), Positives = 213/437 (48%), Gaps = 63/437 (14%)
Query: 49 LRLRLSDELVEDETLNTVEYDSKQESINCKILKAVKGKEFXXXXXXXXXXXXXNEPKVTD 108
++L L +++D+ N YD ++ K K G+ F N+ V
Sbjct: 1 MKLELPGNVLDDDRANA-SYDISSGFVHIKFPKETPGEVFPDLDLLTTLLVNQNKEPVKK 59
Query: 109 GPLIQEISN--------------EKESIAD---IREEGEHFDWEIKQEV---QVDNTPTT 148
PLI+E+ + EKE D I + FDW + Q + ++ T T+
Sbjct: 60 -PLIEEVEDTPSPSTSQNASKDEEKEVKIDWQNIHFDDPDFDWGLLQRLDDEKMQYTSTS 118
Query: 149 KYGFDNLYTGYIVMSVANGNDINELDDPEHTSPENRVKERLKKENLKFDPEYYAAEYITI 208
+YGF+N Y+G + + GN+IN++ +PE T P R + R++ E+ KFD E+Y A++
Sbjct: 119 QYGFNNQYSGLLKYNALVGNEINQIPEPERTPPSTRSEIRVQLEDEKFDAEHYMADFYDR 178
Query: 209 KQGDEEEIQINGIKRLLGYLPPLCERYQQWFERSDNESKERNVSFPVEFTEAEQTQMQDH 268
+ DE +L Y+P Y F +R + F E EA
Sbjct: 179 EMIDE----------ILHYIPD----YIGEFLLVQQGKSDRLLEFTSEEKEALAK----- 219
Query: 269 IPKKKYLINDVKSQYLTILSILYSYTFEQIENEGQHTTESQWTIGKLTPQISMLDQQILL 328
+PK+ YLI++ K+ YL ++ ++++Y ++ G T ES W IG L+ IS LD
Sbjct: 220 LPKRSYLIDNPKNVYLCLVPLIFAYAYDNRTTLGDPTIESCWNIGTLSSTISCLDT---- 275
Query: 329 SESLIHFSQIKGTIVTGIRRALSYPLHRNYDLCIKAWQLAIKLLLGGKRLVIKALLDIHE 388
+F IK + IRR+L+YPL+RN+DL + W+ +L GKR +++ LL I
Sbjct: 276 -----NFLAIKDIFIACIRRSLAYPLYRNWDLALTCWKDTHAILSLGKRWILRVLLRIRN 330
Query: 389 LFRYHDVYYVYNKILLDDLCAWFISQGNDNVIQSLALQAKKELESITKSDIEF------- 441
LF +HDVYY+YN I+L+D W I NDNV+ +LA Q + +ITK + +
Sbjct: 331 LFEHHDVYYIYNAIVLNDYAYW-IQTANDNVLAALAYQVSE--CTITKEETNWPLEEIEA 387
Query: 442 ---ECISGMNEETGEVE 455
E I +EE+ +E
Sbjct: 388 NVQETIIASDEESESIE 404
>Hs8922500 [S] KOG3247 Uncharacterized conserved protein
Length = 519
Score = 152 bits (384), Expect = 1e-36
Identities = 107/345 (31%), Positives = 180/345 (52%), Gaps = 37/345 (10%)
Query: 107 TDGPLIQEI--SNEKESIADIREEGEHFDWEIKQ----EVQVDN-TPTTKYGFDNLYTGY 159
T PL++EI S E + D E FDWEI+Q EV P YGF NL +G
Sbjct: 44 TAKPLVEEIGASEIPEEVVD----DEEFDWEIEQTPCEEVSESALNPQCHYGFGNLRSGV 99
Query: 160 IVMSVANGNDINELDDPEHTSPENRVKERLKKENLKFDPEYYAAEYITIKQGDEEEIQIN 219
+ +D+ ++ DP+ T R ++RL E KFDP++Y A++ E+E
Sbjct: 100 LQRLQDELSDVIDIKDPDFTPAAERRQKRLAAELAKFDPDHYLADFF------EDE---- 149
Query: 220 GIKRLLGYLPPLCERYQQWFERSDNESKERNVSFPVEFTEAEQTQMQDHIPKKKYLINDV 279
I+++L Y P ++Y + + ++ N + V F+E E+ Q++ + K YL++
Sbjct: 150 AIEQILKYNPWWTDKYSKMMAFLEKSQEQENHATLVSFSEEEKYQLRKFV-NKSYLLDKR 208
Query: 280 KSQYL--TILSILYSYTFEQIENEGQHTTESQWTIGKLTPQISMLDQQILLSESLIHFSQ 337
+ + +++ IL +Y +E EG+ ES W I KL+P + + + + ++ F
Sbjct: 209 ACRQVCYSLIDILLAYCYETRVTEGEKNVESAWNIRKLSPTLCWFETWTNVHDIMVSFG- 267
Query: 338 IKGTIVTGIRRALSYPLHRNYDLCIKAWQLAIKLLLGGKRLVIKALLDIHELFRYHDVYY 397
RR L YPL+R++ L +KA++ IK+L GK V+K LLDIH++F+ +D Y
Sbjct: 268 ---------RRVLCYPLYRHFKLVMKAYRDTIKILQLGKSAVLKCLLDIHKIFQENDPAY 318
Query: 398 VYNKILLDDLCAWFISQGNDNVIQSLALQAKKELESITKSDIEFE 442
+ N + + D C W I + + +LA +A KE+ S+TK+ + E
Sbjct: 319 ILNDLYISDYCVW-IQKVKSKKLAALA-EALKEV-SLTKAQLGLE 360
>CE25308_2 [S] KOG3247 Uncharacterized conserved protein
Length = 433
Score = 92.0 bits (227), Expect = 2e-18
Identities = 103/460 (22%), Positives = 194/460 (41%), Gaps = 68/460 (14%)
Query: 1 MITPRFTVTQDDEYIYVQIKVSSIRFNSAGVEVVIDGCMFIFHLSPYYLRLRLSDELVED 60
M+TP F +TQDD+ + ++I+ N A ++ MF+F PY+LRL + E
Sbjct: 1 MLTPVFWITQDDDALLIRIRAP--HGNIAELDYDHGDYMFVFTCPPYFLRLHFKQMVEEY 58
Query: 61 ETLN-TVEYDSKQESINCKILKAVKGKEFXXXXXXXXXXXXXNEPKVTDG-PLIQEISNE 118
+ N +VE+ S + + K+ K K + F G L++E+ +
Sbjct: 59 GSGNGSVEWKSDEGEFHIKVPKMHKKEHFSNLDMITELLTPSTTHHQPHGNQLVEEMDDS 118
Query: 119 KESIADIREEGEHFDWEIKQEVQVDNTPTTK--------YGFDNLYTGYIVMSVANGNDI 170
++ +EG+ ++ ++Q+ + YGF G I I
Sbjct: 119 EDD-----DEGDGSEFLVEQQPAAEPEEPKSDGKIEKFGYGFGWSKFGVIERLRDEIGKI 173
Query: 171 NELDDPEHTSPENRVKERLKKENLKFDPEYYAAEYITIKQGDEEEIQINGIKRLLGYLPP 230
++ +PE+ E R + ++ + FD Y A+ + EEE+ L
Sbjct: 174 VDILEPENVEIEKRADKLMEFDWENFDEGRYLADTLE----PEEEL-----------LAV 218
Query: 231 LCERYQQWFERSDNESKERNVSFPVEFTEAEQTQMQDHIPKK---KYLINDVKSQYLTIL 287
+ ++ Q E SD + +T+++D K K NDV+ +++
Sbjct: 219 ISSKFAQKLEISDED----------------RTKLKDLKKSKTSAKINGNDVEIM-TSLI 261
Query: 288 SILYSYTFEQIENEGQHTTESQWTIGKLTPQISMLDQQILLSESLIHFSQIK--GTIVTG 345
I++ Y ++Q N+ + ES W KL+P +S + FS +K ++
Sbjct: 262 DIVFGYCYDQRVNDWESACESGWNCAKLSPSLSFFAK----------FSSVKEVSCLLAC 311
Query: 346 IRRALSYPLHRNYDLCIKAWQLAIKLLL--GGKRLVIKALLDIHELF-RYHDVYYVYNKI 402
RRAL+YPL+R++ L + Q ++ GG+ ++ L D+H +F + Y+ N +
Sbjct: 312 TRRALTYPLYRSFQLTQRVIQDVCHVITAGGGRPALLHILCDLHRIFIESGEFRYILNDL 371
Query: 403 LLDDLCAWFISQGNDNVIQSLALQAKKELESITKSDIEFE 442
++ D W I D ++ + + I K DI ++
Sbjct: 372 MIADYIFW-IQTVPDEILTRIQTDLTEISGKIDKYDIGWD 410
>7298843 [S] KOG3247 Uncharacterized conserved protein
Length = 608
Score = 70.5 bits (171), Expect = 5e-12
Identities = 81/341 (23%), Positives = 145/341 (41%), Gaps = 58/341 (17%)
Query: 133 DWEIKQ-----EVQVDNTPTTKYGFDNLYTGYIVMSVANGNDINELD-------DPEHTS 180
+W++K+ ++Q+D+ T +N GY + D++ELD +P S
Sbjct: 178 EWKLKRITDQCQLQLDDHERTYKLTNNFGYGY-ASEYSGPLDLSELDRATCRVSEPHRMS 236
Query: 181 PENRVKERLKKENLKFDPEYYAAEYITIKQGDEEEIQI-NGIKRLLGYLPPLCERYQQWF 239
P R +ER+ E +F E Y ++ E+Q+ +G + L Y P + E
Sbjct: 237 PIQRRQERVVDEMKRFSREQYKINFV--------ELQVPSGHQNPLRYKPKIYETSMT-- 286
Query: 240 ERSDNESKERNVSFPVEFTEAEQTQMQDHIPKKKYLINDVKSQYLTILSILYSYTFEQIE 299
+ N+S + Q ++ P + N++ ++SIL + ++
Sbjct: 287 --QEQAHLLHNIS---------RMQTREQQPCDVFRQNEIDCG---LISILLAICYDVRT 332
Query: 300 NEGQHTTESQWTIGKLTPQISMLDQQILLSESLIHFSQIKGTIVTGIRRALSYPLHRNYD 359
+ T ES WT L P +Q F + +V +RR ++YPL+RN++
Sbjct: 333 TNNEPTCESGWTRSILCPLYCYFEQ----------FDNYRDVLVAFLRRMITYPLYRNFE 382
Query: 360 LCIKAWQLAIKLLLGGKRLVIKALLDIHELFRYHDVYY-VYNKILLDDLCAWFISQG--N 416
L + + AI++L GG+ +I LL H+ F D +N L+D + S +
Sbjct: 383 LGRQCVRDAIEVLKGGRNWLINQLLLTHQQFASSDPSRDSFNNYYLEDYIRYVTSPSACS 442
Query: 417 DNVIQSLALQAKKELESITK-------SDIEFECISGMNEE 450
D ++ LA K L + K ++IE E I + +E
Sbjct: 443 DEHMRLLARNLKNVLMDVNKQHLGLGVTEIETELIKELMQE 483
>Hs18575935 [S] KOG3247 Uncharacterized conserved protein
Length = 356
Score = 60.1 bits (144), Expect = 7e-09
Identities = 36/108 (33%), Positives = 63/108 (58%), Gaps = 3/108 (2%)
Query: 335 FSQIKGTIVTGIRRALSYPLHRNYDLCIKAWQLAIKLLLGGKRLVIKALLDIHELFRYHD 394
++ + +V+ RR L Y L+R++ L +KA+ IK+L K V+K LLDIH++F+ +D
Sbjct: 141 WTNVHDIMVSFGRRVLCYLLYRHFKLAMKAYGETIKILQLSKSAVLKCLLDIHKIFQEND 200
Query: 395 VYYVYNKILLDDLCAWFISQGNDNVIQSLALQAKKELESITKSDIEFE 442
Y+ N + + C W + + +LA +A KE+ S+TK+ + E
Sbjct: 201 PAYILNDLYISYYCVW-TQKVKSKTLAALA-EALKEV-SLTKAQLGLE 245
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.136 0.387
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 27,923,774
Number of Sequences: 60738
Number of extensions: 1187456
Number of successful extensions: 3513
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 3488
Number of HSP's gapped (non-prelim): 8
length of query: 475
length of database: 30,389,216
effective HSP length: 110
effective length of query: 365
effective length of database: 23,708,036
effective search space: 8653433140
effective search space used: 8653433140
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)