ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI1074 good J KOG2648 Translation, ribosomal structure and biogenesis Diphthamide biosynthesis protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI1074 374590 375855 422
(422 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YIL103w [J] KOG2648 Diphthamide biosynthesis protein 655 0.0
SPBC3B8.05 [J] KOG2648 Diphthamide biosynthesis protein 459 e-129
7294688 [J] KOG2648 Diphthamide biosynthesis protein 394 e-109
CE17401 [J] KOG2648 Diphthamide biosynthesis protein 391 e-108
Hs4503361 [J] KOG2648 Diphthamide biosynthesis protein 339 4e-93
At5g62030 [J] KOG2648 Diphthamide biosynthesis protein 339 4e-93
ECU07g0980 [J] KOG2648 Diphthamide biosynthesis protein 309 4e-84
SPBC17D1.02 [J] KOG2648 Diphthamide biosynthesis protein 119 1e-26
7299937 [J] KOG2648 Diphthamide biosynthesis protein 104 2e-22
At3g59630 [J] KOG2648 Diphthamide biosynthesis protein 102 1e-21
CE15608 [J] KOG2648 Diphthamide biosynthesis protein 91 4e-18
YKL191w [J] KOG2648 Diphthamide biosynthesis protein 84 5e-16
Hs21536476 [J] KOG2648 Diphthamide biosynthesis protein 55 2e-07
HsM4557533 [J] KOG2648 Diphthamide biosynthesis protein 52 2e-06
>YIL103w [J] KOG2648 Diphthamide biosynthesis protein
Length = 425
Score = 655 bits (1690), Expect = 0.0
Identities = 313/417 (75%), Positives = 359/417 (86%), Gaps = 4/417 (0%)
Query: 5 EVEKKPRRRFVGIKKAAEESDP----SGSTTELVKKIKPKTNIRHAINQIPPELLNDEEL 60
E +K+PRRRF+G K +D + + E++ K K + + ++N +P ++LND+EL
Sbjct: 6 ESKKQPRRRFIGRKSGNSNNDKLTTVAENGNEIIHKQKSRIALGRSVNHVPEDILNDKEL 65
Query: 61 NAAIKLLPSNYNFEIHKTVWNIRKHKAKRVAIQMPEGLLIYSLVISDILEQFCQCEIVVM 120
N AIKLLPSNYNFEIHKTVWNIRK+ AKR+A+QMPEGLLIYSL+ISDILEQFC E +VM
Sbjct: 66 NEAIKLLPSNYNFEIHKTVWNIRKYNAKRIALQMPEGLLIYSLIISDILEQFCGVETLVM 125
Query: 121 GDVSYGACCIDDYTARSLDCDFIVHYAHSCLVPIDITTIKVLYIFVTINIDETHLIKTLQ 180
GDVSYGACCIDD+TAR+LDCDFIVHYAHSCLVPID+T IKVLY+FVTINI E H+IKTLQ
Sbjct: 126 GDVSYGACCIDDFTARALDCDFIVHYAHSCLVPIDVTKIKVLYVFVTINIQEDHIIKTLQ 185
Query: 181 KNFPRGSRIAAFGTIQFNPTIHSMKDKLLQSEEHMLYIIPPQIKPLSKGEVLGCTSERLN 240
KNFP+GSRIA FGTIQFNP +HS++DKLL EEHMLYIIPPQIKPLS+GEVLGCTSERL+
Sbjct: 186 KNFPKGSRIATFGTIQFNPAVHSVRDKLLNDEEHMLYIIPPQIKPLSRGEVLGCTSERLD 245
Query: 241 KEQIDAMVYVGDGRFHLESAMIHNPEIPAFRYDPYNRRFTRERYDQKQLVEVRASAIDKA 300
KEQ DAMV++GDGRFHLESAMIHNPEIPAF+YDPYNR+FTRE YDQKQLVEVRA AI+ A
Sbjct: 246 KEQYDAMVFIGDGRFHLESAMIHNPEIPAFKYDPYNRKFTREGYDQKQLVEVRAEAIEVA 305
Query: 301 RSSKKVGLILGALGRQGNLATVENLETKLKASGRTVVKIILSEIFPQKLAMFDDIDAFVQ 360
R K GLILGALGRQGNL TV+NLE L A+G+TVVKIILSE+FPQKLAMFD ID FVQ
Sbjct: 306 RKGKVFGLILGALGRQGNLNTVKNLEKNLIAAGKTVVKIILSEVFPQKLAMFDQIDVFVQ 365
Query: 361 VACPRLSIDWGYAFNKPLLTPYETNVMLENDRMFNEKYYPMDYYHINGYGRGKVPNH 417
VACPRLSIDWGYAFNKPLLTPYE +V+L+ D MF+EKYYPMDYY GYGRG+ P H
Sbjct: 366 VACPRLSIDWGYAFNKPLLTPYEASVLLKKDVMFSEKYYPMDYYEAKGYGRGETPKH 422
>SPBC3B8.05 [J] KOG2648 Diphthamide biosynthesis protein
Length = 436
Score = 459 bits (1182), Expect = e-129
Identities = 228/429 (53%), Positives = 307/429 (71%), Gaps = 20/429 (4%)
Query: 3 MGEVEKK-PRRRFVGIKKAAEESDPSGSTTEL----VKKIKPKTNIRHAINQIPPELLND 57
M EV+K P+RRFVG KK +E++ GS ++ + I K + QIP ++LND
Sbjct: 1 MAEVKKSIPKRRFVG-KKNRKENNLDGSNRDVENAALVTINSKRSAGRVATQIPEDILND 59
Query: 58 EELNAAIKLLPSNYNFEIHKTVWNIRKHKAKRVAIQMPEGLLIYSLVISDILEQFCQCEI 117
+ +N AIKLLP NYNFEIHKT+W+IR KAKRVA+Q+PEGLL++ ++SDI EQFCQ E
Sbjct: 60 KAINEAIKLLPQNYNFEIHKTIWHIRLRKAKRVALQLPEGLLMFGCILSDIFEQFCQVET 119
Query: 118 VVMGDVSYGACCIDDYTARSLDCDFIVHYAHSCLVPIDITTIKVLYIFVTINIDETHLIK 177
+VMGDV+YGACCIDD+TAR+LDCDF+VHY HSCL+P+D T IKVLY+FV I ID H++
Sbjct: 120 IVMGDVTYGACCIDDFTARALDCDFLVHYGHSCLIPVDQTPIKVLYVFVDIKIDLQHVVS 179
Query: 178 TLQKNFPRGSRIAAFGTIQFNPTIHSMKDKL-LQSEEHM--LYIIPPQIKPLSKGEVLGC 234
+L+ N P SR+A GTIQF +++S+KD L +Q E+ Y++ PQ KPLS GE LGC
Sbjct: 180 SLKHNLPSNSRLALVGTIQFVGSLNSIKDALQIQDEDGKGGFYVVIPQAKPLSPGEALGC 239
Query: 235 TSERLNKEQIDAMVYVGDGRFHLESAMIHNPEIPAFRYDPYNRRFTRERYDQKQLVEVRA 294
TS + K +DA++Y+GDGRFHLES MI NP++PA+RYDPY+ + + E Y +++ +R
Sbjct: 240 TSPYIEKGSVDALIYIGDGRFHLESVMIANPDLPAYRYDPYSHKLSIESYAHEEMKSIRY 299
Query: 295 SAIDKARSSKKVGLILGALGRQGNLATVENLETKLKASGRTVVKIILSEIFPQKLAMFDD 354
SA++KAR++KK GLI G LGRQG+ +ENL+ L+ + + V +++SEIFP +L F D
Sbjct: 300 SAVEKARTAKKFGLIQGTLGRQGSPKVLENLKNTLRKNNKDFVCVLMSEIFPSRLGQFSD 359
Query: 355 IDAFVQVACPRLSIDWGYAFNKPLLTPYETNVMLENDRMFN----EKYYPMDYYHINGYG 410
IDA++QVACPRLSIDWGYAF PLLTPYE + FN ++ YPMD+Y N G
Sbjct: 360 IDAWIQVACPRLSIDWGYAFPAPLLTPYEASA------AFNVVPWKEVYPMDFYATNSLG 413
Query: 411 RGKVPNHDD 419
PN+ +
Sbjct: 414 NW-TPNNPE 421
>7294688 [J] KOG2648 Diphthamide biosynthesis protein
Length = 454
Score = 394 bits (1013), Expect = e-109
Identities = 204/400 (51%), Positives = 273/400 (68%), Gaps = 23/400 (5%)
Query: 26 PSGSTTELV-------KKIKPKTNIRHAINQIPPELLNDEELNAAIKLLPSNYNFEIHKT 78
P+ +T++V K KPK I N+IP ELL+D L AI LPSNYNFE+HKT
Sbjct: 12 PTSESTDIVVVRARPKKVFKPKARI----NRIPQELLDDPLLQQAIDRLPSNYNFEMHKT 67
Query: 79 VWNIRKHKAKRVAIQMPEGLLIYSLVISDILEQFCQCEIVVMGDVSYGACCIDDYTARSL 138
+W IR+ KAKRVA+Q+PEGLL+Y++VISDI+E+F + V+MGDV+YGACC+DDYTA++L
Sbjct: 68 IWRIREMKAKRVALQLPEGLLMYAMVISDIVERFTSADTVIMGDVTYGACCVDDYTAKAL 127
Query: 139 DCDFIVHYAHSCLVPIDITT-IKVLYIFVTINIDETHLIKTLQKNF-PRGSRIAAFGTIQ 196
D +VHY HSCL+P+D T IKVLYIFV I ID H + +++ NF P +IA TIQ
Sbjct: 128 GADLLVHYGHSCLIPVDQTCGIKVLYIFVDIKIDPLHFLDSVKLNFRPEVGQIALVSTIQ 187
Query: 197 FNPTIHSMKDKLLQSEEHMLYIIPPQIKPLSKGEVLGCTSERLNKEQIDAMVYVGDGRFH 256
F T+ + +L + +I PQ KPLS GE+LGCTS +L E ++Y+GDGRFH
Sbjct: 188 FVTTLQAASTELKAAGYD---VIVPQAKPLSPGEILGCTSPQL-PETTKMIIYLGDGRFH 243
Query: 257 LESAMIHNPEIPAFRYDPYNRRFTRERYDQKQLVEVRASAIDKARSSKKVGLILGALGRQ 316
LESAMI NP + A++YDPY ++FT E+YD + +R A+ +A+ ++++G+ILG LGRQ
Sbjct: 244 LESAMIANPLLKAYKYDPYEKKFTTEQYDHSAMQNLRLDAVQRAKKARRIGIILGTLGRQ 303
Query: 317 GNLATVENLETKLKASGRTVVKIILSEIFPQKLAMFDDIDAFVQVACPRLSIDWGYAFNK 376
G+ LE +L A G ++LSEIFPQKLA+F DIDAFVQ+ACPRLSIDWG AF++
Sbjct: 304 GSSRVHRFLEKRLHAKGIETTTLLLSEIFPQKLALFADIDAFVQIACPRLSIDWGSAFSQ 363
Query: 377 PLLTPYETNVML------ENDRMFNEKYYPMDYYHINGYG 410
PLL PYE +V+L ++ YPMD+Y G
Sbjct: 364 PLLNPYELSVVLGDVEWTPHNSSPRNNAYPMDFYASGSLG 403
>CE17401 [J] KOG2648 Diphthamide biosynthesis protein
Length = 396
Score = 391 bits (1004), Expect = e-108
Identities = 191/365 (52%), Positives = 264/365 (72%), Gaps = 4/365 (1%)
Query: 47 INQIPPELLNDEELNAAIKLLPSNYNFEIHKTVWNIRKHKAKRVAIQMPEGLLIYSLVIS 106
I+ + E+ N+ L +K+LPSNY FE+ KT+W IR ++K VA+Q PEGL++Y+ VI+
Sbjct: 8 ISTLVEEIANNPNLKEDLKILPSNYTFEVPKTIWKIRSTESKYVALQFPEGLIMYACVIA 67
Query: 107 DILEQFCQCEIVVMGDVSYGACCIDDYTARSLDCDFIVHYAHSCLVPIDITT-IKVLYIF 165
DILE++ C+ V+MGDV+YGACC+DDYTA+S+ CD +VHY HSCLVPI T I +LY+F
Sbjct: 68 DILEKYTGCDTVIMGDVTYGACCVDDYTAKSMGCDLLVHYGHSCLVPIQNTDGIAMLYVF 127
Query: 166 VTINIDETHLIKTLQKNFPRGSRIAAFGTIQFNPTIHSMKDKLLQSEEHMLYIIPPQIKP 225
V I+I+ +HLI +++NF +G R+ T+QF P++ +++ ++ + I PQ KP
Sbjct: 128 VNIHINLSHLIDCVKENF-QGKRLVVVSTVQFIPSLQTLRTTF-NKDDSSIRIDIPQCKP 185
Query: 226 LSKGEVLGCTSERLNKEQIDAMVYVGDGRFHLESAMIHNPEIPAFRYDPYNRRFTRERYD 285
LS GEVLGCTS RL+ + DA+VY+GDGRFHLES MIHNPEI AF+YDPY+R+ TRE YD
Sbjct: 186 LSPGEVLGCTSPRLDASKYDAIVYLGDGRFHLESIMIHNPEIEAFQYDPYSRKLTREFYD 245
Query: 286 QKQLVEVRASAIDKARSSKKVGLILGALGRQGNLATVENLETKLKASGRTVVKIILSEIF 345
+ + R +I+ AR GLI G LGRQGNL VE LE +L+ G+ ++++LSEIF
Sbjct: 246 HDLMRKNRIGSIEIARKCTTFGLIQGTLGRQGNLKVVEELEAQLERKGKKFLRVLLSEIF 305
Query: 346 PQKLAMFDDIDAFVQVACPRLSIDWGYAFNKPLLTPYETNVMLENDRMFNEKYYPMDYYH 405
P+KLAMF ++D +VQVACPRLSIDWG F KPLL P+E V L+N + ++ ++PMDYY
Sbjct: 306 PEKLAMFPEVDCWVQVACPRLSIDWGTQFPKPLLYPFELAVALDNISVPSD-HWPMDYYS 364
Query: 406 INGYG 410
+ G
Sbjct: 365 NDSLG 369
>Hs4503361 [J] KOG2648 Diphthamide biosynthesis protein
Length = 363
Score = 339 bits (870), Expect = 4e-93
Identities = 171/328 (52%), Positives = 236/328 (71%), Gaps = 9/328 (2%)
Query: 94 MPEGLLIYSLVISDILEQFCQCEIVVMGDVSYGACCIDDYTARSLDCDFIVHYAHSCLVP 153
MPEGLL+++ I DILE+F + E++VMGDV+YGACC+DD+TAR+L DF+VHY HSCL+P
Sbjct: 1 MPEGLLLFACTIVDILERFTEAEVMVMGDVTYGACCVDDFTARALGADFLVHYGHSCLIP 60
Query: 154 IDITT--IKVLYIFVTINIDETHLIKTLQKNFPRGSRIAAFGTIQFNPTIHSMKDKLLQS 211
+D + +VLY+FV I ID THL+ +L+ FP + +A TIQF T+ + +L
Sbjct: 61 MDTSAQDFRVLYVFVDIRIDTTHLLDSLRLTFPPATALALVSTIQFVSTLQAAAQEL--K 118
Query: 212 EEHMLYIIPPQIKPLSKGEVLGCTSERLNKEQIDAMVYVGDGRFHLESAMIHNPEIPAFR 271
E+ + + PQ KPLS GE+LGCTS RL+KE ++A+VY+GDGRFHLES MI NP +PA+R
Sbjct: 119 AEYRVSV--PQCKPLSPGEILGCTSPRLSKE-VEAVVYLGDGRFHLESVMIANPNVPAYR 175
Query: 272 YDPYNRRFTRERYDQKQLVEVRASAIDKARSSKKVGLILGALGRQGNLATVENLETKLKA 331
YDPY++ +RE YD +++ R AI ARS+K GLILG LGRQG+ +E+LE++L+A
Sbjct: 176 YDPYSKVLSREHYDHQRMQAARQEAIATARSAKSWGLILGTLGRQGSPKILEHLESRLRA 235
Query: 332 SGRTVVKIILSEIFPQKLAMFDDIDAFVQVACPRLSIDWGYAFNKPLLTPYETNVMLEND 391
G + V+++LSEIFP KL++ ++D +VQVACPRLSIDWG A KPLLTPYE V L +
Sbjct: 236 LGLSFVRLLLSEIFPSKLSLLPEVDVWVQVACPRLSIDWGTASPKPLLTPYEAAVALRD- 294
Query: 392 RMFNEKYYPMDYYHINGYGRGKVPNHDD 419
+ ++ YPMD+Y + G V + D
Sbjct: 295 -ISWQQPYPMDFYAGSSLGPWTVNHGQD 321
>At5g62030 [J] KOG2648 Diphthamide biosynthesis protein
Length = 453
Score = 339 bits (870), Expect = 4e-93
Identities = 183/375 (48%), Positives = 245/375 (64%), Gaps = 17/375 (4%)
Query: 22 EESDPSGSTTELVKKIKPKTNIRHAINQIPPELLNDEELNAAIKLLPSNYNFEIHKTVWN 81
E SDP+ T K+ PK I+ NQIP +L D LNAAI +LPS Y+FE+HK VW
Sbjct: 2 ELSDPNNGKTNKPKQ-PPKRFIK---NQIPDSILGDASLNAAISILPSIYHFEVHKCVWR 57
Query: 82 IRKHKAKRVAIQMPEGLLIYSLVISDILEQFCQCE-IVVMGDVSYGACCIDDYTARSLDC 140
I+ AKR+A+Q+PEGLL+Y+L +SDI F V+GDV+YGACC+DD++A +L
Sbjct: 58 IKTSNAKRIALQLPEGLLMYALTLSDIFTSFAGASHCFVLGDVTYGACCVDDFSACALGA 117
Query: 141 DFIVHYAHSCLVPIDITTIKVLYIFVTINIDETHLIKTLQKNFPRGSR-IAAFGTIQFNP 199
D ++HY HSCLVPID T I LY+FV I ID L+ T+ N + I GTIQF
Sbjct: 118 DLLIHYGHSCLVPIDSTKIPCLYVFVEIQIDVKCLLNTIHLNLASDVKNIILAGTIQFTS 177
Query: 200 TIHSMKDKLLQSEEHMLYIIPPQIKPLSKGEVLGCTSERLN-----KEQIDAMVYVGDGR 254
I ++K +L E+ ++ PQ KPLS GEVLGCT+ ++ K+Q+ +V+V DGR
Sbjct: 178 AIRAVKPEL---EKQGFNVLIPQSKPLSAGEVLGCTAPKVKTVEDCKDQV--LVFVADGR 232
Query: 255 FHLESAMIHNPEIPAFRYDPYNRRFTRERYDQKQLVEVRASAIDKARSSKKVGLILGALG 314
FHLE+ MI NP+I AFRYDPY + E YD K + E R AI +AR +K G++LG LG
Sbjct: 233 FHLEAFMIANPKIKAFRYDPYLGKLFLEEYDHKGMRETRMRAIARAREAKTWGIVLGTLG 292
Query: 315 RQGNLATVENLETKLKASGRTVVKIILSEIFPQKLAMFDD-IDAFVQVACPRLSIDWGYA 373
RQGN +E LE K+ G +++SE+ P ++A+F+D +DA+VQ+ACPRLSIDWG A
Sbjct: 293 RQGNPKILERLEKKMMEKGIDSTVVLMSELSPTRVALFEDSVDAWVQIACPRLSIDWGEA 352
Query: 374 FNKPLLTPYETNVML 388
F KPLLT +E + L
Sbjct: 353 FLKPLLTTFEAEIAL 367
>ECU07g0980 [J] KOG2648 Diphthamide biosynthesis protein
Length = 414
Score = 309 bits (792), Expect = 4e-84
Identities = 165/360 (45%), Positives = 229/360 (62%), Gaps = 18/360 (5%)
Query: 54 LLNDEELNAAIKLLPSNYNFEIHKTVWNIRKHKAKRVAIQMPEGLLIYSLVISDILEQFC 113
+L EE+ ++ LP NYNFE K + IR+ AKRV +Q P+GLL YS VI D++E++
Sbjct: 70 VLKQEEVPECLRYLPRNYNFETGKVLRTIRRLGAKRVTLQFPDGLLRYSFVIMDVIEKYT 129
Query: 114 QCEIVVMGDVSYGACCIDDYTARSLDCDFIVHYAHSCLVPIDITTIKVLYIFVTINIDET 173
E +++ DV YG CCIDD S+ D +VHY HSCLVP+ + KVLYIFV I ID
Sbjct: 130 GAECILLNDVVYGGCCIDD---ESIASDLLVHYGHSCLVPVGEMSTKVLYIFVDIRIDID 186
Query: 174 HLIKTLQKNFPRGSRIAAFGTIQFNPTIHSMKDKLLQSEEHMLYIIPPQIKPLSKGEVLG 233
H + +++NF +I GTIQFN +I+ +K ++L E + PQI+PLS GEVLG
Sbjct: 187 HAAEMIRRNFQ--GKIGVIGTIQFNSSINRLK-RVLDEERGGVECTLPQIRPLSSGEVLG 243
Query: 234 CTSERLNKEQIDAMVYVGDGRFHLESAMIHNPEIPAFRYDPYNRRFTRERYDQKQLVEVR 293
CT+ ++ E + A++ +GDGRFHLE AMI NP + ++Y P++RR T+E YD ++ R
Sbjct: 244 CTAPKI--EGVSAVISIGDGRFHLEGAMIRNPHLRFYKYCPFSRRMTQESYDHSTMLSDR 301
Query: 294 ASAIDKARSSKKVGLILGALGRQGNLATVENLETKLKASGRTVVKIILSEIFPQKLAMFD 353
S I KA S + G+ILG+LGRQGN + + ++ +LK V I+L EI P+KL ++
Sbjct: 302 KSEIRKAFSGRSFGVILGSLGRQGNRSILRSVVDRLKE--YDVYLIMLDEISPKKLERYN 359
Query: 354 DIDAFVQVACPRLSIDWGYAFNKPLLTPYETNVMLENDRMFNEKYYPMDYYHINGYGRGK 413
ID+FVQ++CPRLSIDWG F KPLLTP+E ++ Y MDYY G G K
Sbjct: 360 FIDSFVQISCPRLSIDWGKLFKKPLLTPFEV--------FYSGGEYLMDYYSREGSGEWK 411
>SPBC17D1.02 [J] KOG2648 Diphthamide biosynthesis protein
Length = 503
Score = 119 bits (297), Expect = 1e-26
Identities = 90/362 (24%), Positives = 165/362 (44%), Gaps = 24/362 (6%)
Query: 53 ELLNDEELNAAIKLLPSNYN-FEIHKTVWNIRKHKAKRVAIQMPEGLLIYSLVISDILEQ 111
E + ++E+ +K+ +EI++TV I+ VA+Q P+ L S ++ IL
Sbjct: 16 EAILEKEIGETVKIEGDLVEVYEINRTVDFIKSGNYNSVALQFPDEHLADSGKVASILTN 75
Query: 112 FCQCEIVVMGDVSYGACCIDDYTARSLDCDFIVHYAHSCLVPIDITTIKVLYIFVTINID 171
+ + ++ D +YG+CC+D+ A + D IVHY +CL P + + VLY+F + I+
Sbjct: 76 LVEANVQILADTNYGSCCVDEVAAEHMSADAIVHYGRACLSP--TSRLPVLYVFGRLPIN 133
Query: 172 ETHLIKTLQKNFPRGSRIAAFGTIQFNPTIHSMKDKLLQS----------EEHMLYIIPP 221
L K L P I ++ + +D +L+S E H+ I P
Sbjct: 134 LHKLEKCL--TIPLDQNILLVSDTRW----YYAQDSILKSLKTLGYQNVYESHLKERIEP 187
Query: 222 QIKPLSKGEVLGCTSERLNKE---QIDAMVYVGDGRFHLES-AMIHNPEIPAF-RYDPYN 276
++ S + + L K Q ++Y+G L S M H + F +DP +
Sbjct: 188 NLEEASTSYTIPGRTYSLPKSLSLQDMTLLYIGPDSPTLSSILMSHYSLVNQFLSFDPLS 247
Query: 277 RRFTRERYDQKQLVEVRASAIDKARSSKKVGLILGALGRQGNLATVENLETKLKASGRTV 336
+ E + R + + + R + +G+++G LG L + L + +G+
Sbjct: 248 NKIVEESSFTGAKLRRRYALVQRCRDAGVIGIVIGTLGVHRYLHVLNQLRKMILNAGKKP 307
Query: 337 VKIILSEIFPQKLAMFDDIDAFVQVACPRLSIDWGYAFNKPLLTPYETNVMLENDRMFNE 396
+ + ++ P KLA F +I+ FV +AC S+ F +P++TP+E L +D +N
Sbjct: 308 YMLAVGKLNPAKLANFQEIECFVLIACGENSLIDSKEFYRPIVTPFELVKALSSDMSWNN 367
Query: 397 KY 398
+
Sbjct: 368 DF 369
>7299937 [J] KOG2648 Diphthamide biosynthesis protein
Length = 469
Score = 104 bits (260), Expect = 2e-22
Identities = 91/359 (25%), Positives = 163/359 (45%), Gaps = 26/359 (7%)
Query: 70 NYNFEIHKTVWNIRKHKAKRVAIQMPEGLLIYSLVISDILEQFCQCE---IVVMGDVSYG 126
N N W IRK KRV +Q P+ L +S IS L++ E + ++ D +YG
Sbjct: 36 NENHRRSSVDW-IRKSGYKRVCLQFPDDYLPHSNAISVGLKELLAPEDVKVFILADTTYG 94
Query: 127 ACCIDDYTARSLDCDFIVHYAHSCLVPIDITTIKVLYIFVTINIDETHLIKTLQ----KN 182
+CC+D+ A +D D ++H+ ++C + VLY++ + +D L+ LQ ++
Sbjct: 95 SCCVDEIAAAHVDADSVIHFGNACRSRA--ARLPVLYLYPELPLDVNVLLSKLQGLRAES 152
Query: 183 FPRGSRIAAFGTIQFNPT----IHSMKDKLLQSEEHMLYIIPPQIKPLSKGEVLGCTSER 238
R ++ + I + + ++L+ +E +L + PP I+ S T E
Sbjct: 153 IDR--QVCVYLDIGYQHVYGEKLKKQLSEILEPKELLLELFPP-IEAEST-----TTRET 204
Query: 239 LNKEQIDAMVYVGDGRFHLESAMIHNPEIPAFR-YDPYNRRFTRERYDQKQLVEVRASAI 297
N E+I +++G + + P + YD + + + Q + R I
Sbjct: 205 TNLERI--CIFIGADNQRFANLSLTAPAAVQWHIYDGSSDSLSSKNPLTAQFIRRRYFHI 262
Query: 298 DKARSSKKVGLILGALGRQGNLATVENLETKLKASGRTVVKIILSEIFPQKLAMFDDIDA 357
+K + ++ +GLI+ L +G L V L+T K G I + I P KLA F +ID
Sbjct: 263 EKCKDAQTLGLIVATLSAEGYLDVVTRLQTMAKGRGIKTQLISVGRINPAKLANFLEIDC 322
Query: 358 FVQVACPRLSIDWGYAFNKPLLTPYETNVMLEND-RMFNEKYYPMDYYHINGYGRGKVP 415
FV + CP ++ + KP+++ +E + L M + Y D+ + GR +P
Sbjct: 323 FVLIGCPFNNMYDSKEYYKPIVSVFEAEMALNPAWHMRYPEAYVTDFKQLLPEGRSFLP 381
>At3g59630 [J] KOG2648 Diphthamide biosynthesis protein
Length = 491
Score = 102 bits (253), Expect = 1e-21
Identities = 78/350 (22%), Positives = 145/350 (41%), Gaps = 36/350 (10%)
Query: 73 FEIHKTVWNIRKHKAKRVAIQMPEGLLIYSLVISDILEQFC------QCEIVVMGDVSYG 126
+EI++T I R+A+Q P+ LL S + L+ + VM D +YG
Sbjct: 9 YEINRTAEFIISKSFTRIALQFPDELLKDSTKVVSALKSKTRLLTDREVRFFVMADTTYG 68
Query: 127 ACCIDDYTARSLDCDFIVHYAHSCLVPIDITTIKVLYIFVTINIDET--HLIKTLQKNFP 184
+CCID+ A +D + +VHY +CL P + ++ +IN+ HLI K+
Sbjct: 69 SCCIDEVGALHIDSECVVHYGQTCLSPTSVLPAFFVFGKASINVSSCVKHLIDYASKSDK 128
Query: 185 RGSRIAAFGTIQFNPTIH-----SMKDKLLQSEEHMLYIIPPQIKPLSKGEVLGCTSERL 239
+ P I S D L + I P P E SE
Sbjct: 129 PIMILYGLEYAHVIPQIREELGLSKTDSQLSVANVLCSFISPSKDPRESMEHPRPYSESD 188
Query: 240 NKEQIDA-----------------------MVYVGDGRFHLESAMIHNPEIPAFRYDPYN 276
+ + + + + ++G + ++ RYD
Sbjct: 189 SSDSLSSSRSYRLGGLTWDLPEGSKIEDYLLFWIGSDSSAFANVVLTFNGCDIVRYDAEE 248
Query: 277 RRFTRERYDQKQLVEVRASAIDKARSSKKVGLILGALGRQGNLATVENLETKLKASGRTV 336
E Y Q+++++ R ++KA+ + +G+++G LG G L + +++ + A+G+
Sbjct: 249 DSLVTEFYQQRRILKRRYYLVEKAKDANIIGILVGTLGVAGYLHMIHHMQALISAAGKKS 308
Query: 337 VKIILSEIFPQKLAMFDDIDAFVQVACPRLSIDWGYAFNKPLLTPYETNV 386
+ + P KLA F + D F+ ++C + ++ F P++TP+E N+
Sbjct: 309 YILAMGRPNPAKLANFPECDVFIYISCAQTALLDSKEFMSPVITPFEANL 358
>CE15608 [J] KOG2648 Diphthamide biosynthesis protein
Length = 476
Score = 90.5 bits (223), Expect = 4e-18
Identities = 79/339 (23%), Positives = 150/339 (43%), Gaps = 31/339 (9%)
Query: 73 FEIHKTVWNIRKHKAKRVAIQMPEGLLIYSLVISDILEQFCQCEIV----VMGDVSYGAC 128
FEI T I++ +R+A+Q P+ LL YS ++ ++E + E V+ D SY +C
Sbjct: 42 FEIDATSEWIKQGNHQRIALQFPDSLLPYSEKVTKLIESRFRSESAKKTFVLADTSYRSC 101
Query: 129 CIDDYTARSLDCDFIVHYAHSC-LVPIDITTIKVLYIFVTINIDE--------------T 173
C+D+ A DC +VH+ +C P D +K + + I ID+
Sbjct: 102 CVDEVAAAHADCTALVHFGEACHSAPTDKIDVKYVLGSMPIFIDQFRMEFQNVADQLTAE 161
Query: 174 HLIKTLQKNFPRGSRIAAFGTIQFNPTIHSMKDKLLQSEEHMLYIIPPQIKPLSKG-EVL 232
H++ + F A + P ++ LL SE+ ++ + + G E+
Sbjct: 162 HIVLLMDSCFSHEQEKVAAVIKEVLPGNRHVECSLLPSED----VLKQNRQNIYLGREIP 217
Query: 233 GCTSERLNKEQIDAMVYVGDGRFHLESA-MIHNPEIPAF-RYDPYNRRFTRERYDQKQLV 290
C L Q +++ G L ++ P ++P N+ E +L+
Sbjct: 218 SC----LRNNQPTDLIFCGFPNSPLLPIWLLSYPSCSTVSHFNPINKTIQHESTRSSRLL 273
Query: 291 EVRASAIDKARSSKKVGLILGALGRQGNLATVENLETKLKASGRTVVKIILSEIFPQKLA 350
R ++K + + VGL++G++G + V+ + K +G+ + I + +I KL+
Sbjct: 274 RKRLFLVEKLKDADTVGLVVGSVGVDKHREAVKRMREMCKKAGKKIYVISVGKINVPKLS 333
Query: 351 MFD-DIDAFVQVACPRLSIDWGYAFNKPLLTPYETNVML 388
F DID FV ++CP + + +P+++ +E + L
Sbjct: 334 NFSTDIDVFVLLSCPFGVVLDSSDYFRPVVSYFEAEIAL 372
>YKL191w [J] KOG2648 Diphthamide biosynthesis protein
Length = 534
Score = 83.6 bits (205), Expect = 5e-16
Identities = 86/385 (22%), Positives = 164/385 (42%), Gaps = 62/385 (16%)
Query: 56 NDEELNAAIKLLPSNYNFEIHKTVWNIRKH-KAKRVAIQMPEGLLIYSLVISDILEQ--- 111
N +EL ++L+ + YN E V + +H + + V +Q P+ L+ S +I +L+
Sbjct: 35 NSDEL---MQLISAYYNVE--PLVGYLEQHPEYQNVTLQFPDDLIKDSSLIVRLLQSKFP 89
Query: 112 FCQCEIVVMGDVSYGACCIDDYTARSLDCDFIVHYAHSCLVPIDITTIKVLYIFVTINID 171
+ + V+ D +Y ACC+D+ A + + +VH+ +CL I + V+Y F T +D
Sbjct: 90 HGKIKFWVLADTAYSACCVDEVAAEHVHAEVVVHFGDACLNAIQ--NLPVVYSFGTPFLD 147
Query: 172 ETHLIKTLQKNFP-RGSRIAAFGTIQFNPTIHSMKDKLLQSEEHMLYIIPPQIKPLSKGE 230
+++ Q+ FP S+I F+ + + + +L+ + H II Q+ + E
Sbjct: 148 LALVVENFQRAFPDLSSKICLMANAPFSKHLSQLYN-ILKGDLHYTNIIYSQVNTSAVEE 206
Query: 231 VLGCTSERLN-KEQIDAM-------VYVGD------------GRFHL------------- 257
+ + E +D + V G FHL
Sbjct: 207 KFVTILDTFHVPEDVDQVGVFEKNSVLFGQHDKADNISPEDYHLFHLTTPQDPRLLYLST 266
Query: 258 --ESAMIHNPEIPAFRYDPYNRRFTRERYDQKQLVEVRASAIDKARSSKKVGLILGALGR 315
+S I +P +P P+ R +Y + AR++ +G+++ L
Sbjct: 267 VFQSVHIFDPALPGMVTGPFPSLMRRYKY------------MHVARTAGCIGILVNTLSL 314
Query: 316 QGNLATVENLETKLKASGRTVVKIILSEIFPQKLAMFDDIDAFVQVACPR--LSIDWGYA 373
+ T+ L +K + ++ + KLA F+DID + + C + + +D
Sbjct: 315 RNTRETINELVKLIKTREKKHYLFVVGKPNVAKLANFEDIDIWCILGCSQSGIIVDQFNE 374
Query: 374 FNKPLLTPYETNVMLENDRMFNEKY 398
F KP++TPYE N+ L + + K+
Sbjct: 375 FYKPIITPYELNLALSEEVTWTGKW 399
>Hs21536476 [J] KOG2648 Diphthamide biosynthesis protein
Length = 489
Score = 55.1 bits (131), Expect = 2e-07
Identities = 23/81 (28%), Positives = 46/81 (56%)
Query: 73 FEIHKTVWNIRKHKAKRVAIQMPEGLLIYSLVISDILEQFCQCEIVVMGDVSYGACCIDD 132
+E+ + +R +RVA+Q P+ LL ++ ++ LE+ ++ ++GD +YG+CC+D
Sbjct: 33 YELERVAGFVRDLGCERVALQFPDQLLGDAVAVAARLEETTGSKMFILGDTAYGSCCVDV 92
Query: 133 YTARSLDCDFIVHYAHSCLVP 153
A ++H+ +CL P
Sbjct: 93 LGAEQAGAQALIHFGPACLSP 113
Score = 52.4 bits (124), Expect = 1e-06
Identities = 30/93 (32%), Positives = 50/93 (53%), Gaps = 2/93 (2%)
Query: 293 RASAIDKARSSKKVGLILGALGRQGNLATVENLETKLKASGRTVVKIILSEIFPQKLAMF 352
R +++AR ++ VGL+ G LG + + +L +A+G+ + L P KLA F
Sbjct: 271 RRYLVERARDARVVGLLAGTLGVAQHREALAHLRNLTQAAGKRSYVLALGRPTPAKLANF 330
Query: 353 DDIDAFVQVACP--RLSIDWGYAFNKPLLTPYE 383
++D FV +ACP L+ +F +P+L P E
Sbjct: 331 PEVDVFVLLACPLGALAPQLSGSFFQPILAPCE 363
>HsM4557533 [J] KOG2648 Diphthamide biosynthesis protein
Length = 489
Score = 52.0 bits (123), Expect = 2e-06
Identities = 22/80 (27%), Positives = 44/80 (54%)
Query: 74 EIHKTVWNIRKHKAKRVAIQMPEGLLIYSLVISDILEQFCQCEIVVMGDVSYGACCIDDY 133
E+ + +R +R A+Q P+ LL ++ ++ LE+ ++ ++GD +YG+CC+D
Sbjct: 34 ELERVAGFVRDLGCERXALQFPDQLLGDAVAVAAXLEETTGSKMFILGDTAYGSCCVDVL 93
Query: 134 TARSLDCDFIVHYAHSCLVP 153
A ++H+ +CL P
Sbjct: 94 GAEQAGXQALIHFGPACLSP 113
Score = 50.8 bits (120), Expect = 4e-06
Identities = 29/93 (31%), Positives = 49/93 (52%), Gaps = 2/93 (2%)
Query: 293 RASAIDKARSSKKVGLILGALGRQGNLATVENLETKLKASGRTVVKIILSEIFPQKLAMF 352
R +++AR ++ VGL+ G G + + +L +A+G+ + L P KLA F
Sbjct: 271 RRYXVERARDARVVGLLAGTXGVAQHREALAHLRNLTQAAGKRSYVLALGRPTPAKLANF 330
Query: 353 DDIDAFVQVACP--RLSIDWGYAFNKPLLTPYE 383
++D FV +ACP L+ +F +P+L P E
Sbjct: 331 PEVDVFVLLACPLGALAPQLSGSFFQPILAPCE 363
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.321 0.139 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,681,694
Number of Sequences: 60738
Number of extensions: 1095938
Number of successful extensions: 2621
Number of sequences better than 1.0e-05: 14
Number of HSP's better than 0.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 2573
Number of HSP's gapped (non-prelim): 19
length of query: 422
length of database: 30,389,216
effective HSP length: 109
effective length of query: 313
effective length of database: 23,768,774
effective search space: 7439626262
effective search space used: 7439626262
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)