ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI1078 good S KOG3758 Function unknown Uncharacterized conserved protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI1078 378254 375918 -779
(779 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YNL041c [S] KOG3758 Uncharacterized conserved protein 513 e-145
Hs18582141 [S] KOG3758 Uncharacterized conserved protein 92 4e-18
SPBC776.10c [S] KOG3758 Uncharacterized conserved protein 81 7e-15
At1g31780 [S] KOG3758 Uncharacterized conserved protein 81 7e-15
CE07351 [S] KOG3758 Uncharacterized conserved protein 74 7e-13
7303912 [S] KOG3758 Uncharacterized conserved protein 74 9e-13
>YNL041c [S] KOG3758 Uncharacterized conserved protein
Length = 839
Score = 513 bits (1322), Expect = e-145
Identities = 280/773 (36%), Positives = 464/773 (59%), Gaps = 54/773 (6%)
Query: 54 DLHSKMKHFASISLGEIR-----------------------------PASVSLSKEVAAP 84
DLH KM H+A S+ +I+ P ++ LSK V+
Sbjct: 71 DLHKKMTHYAMSSIDKIQLSNPSKQLGQNSQDEKLSQQESQNFTNYEPKNLDLSKLVSPS 130
Query: 85 ISNNIEKPTTTNQLLSKKINSLLNVPNYASYTSDSETKRALQILDANHSDLGLNFNQLVA 144
+N TTN +LS K++ +LN +Y + + +++L++L+ N L L+ +L+
Sbjct: 131 SGSN---KNTTNLVLSNKLSKILNNYTLINYQATVQLRKSLKVLEENKERLSLDEQKLMN 187
Query: 145 SDFVGTLNRKSLRSKLENSMLNSHSDILSNFQSIARRIKRLSGPLERINNAMQ------- 197
++VGTL R++LR+ LE+ +L H +L F+ I RRIKRLS +E+I +
Sbjct: 188 PEYVGTLARRALRTDLESQLLKEHITVLEEFKPIIRRIKRLSSSVEKIQRTSEKLLSNET 247
Query: 198 NFKDTTESIDFEFEHVKDRLDQLKARRQVIIKLRDSLTLTQLELDHLQNGTINDLFFQIL 257
N T + E + + + +QLK ++++++ +RD TL Q+E D + NGTI+++FF+++
Sbjct: 248 NEVPTNNVVLQEIDQYRLKAEQLKLKKKILLSIRDRFTLNQVEDDVITNGTIDNIFFEVV 307
Query: 258 NKINFIKEKATYLLSNEKTTSAGVALLKTMNNNLTVSNKRIYNYLINFIEEYDTLSRQYG 317
K+ IK+++++LL+ +AG AL+ +N L +NK+I+NYLI+F+ +++ S
Sbjct: 308 KKVINIKDESSFLLT-LPNLNAGNALIMGVNEILEKTNKKIFNYLIDFLYSFESSSNLLN 366
Query: 318 ER-TIGDESLSNFQTSLIHLSNDVQFFQDFLNRIVGLRSKRLLDDFLSQFDIDNKKLQRP 376
+ T ESL+ F+ SL+ LS+D++ F + L R+ LRSK +LD+FLSQFD+ N +P
Sbjct: 367 DHGTTEQESLNIFRKSLVFLSSDLELFNELLKRVTTLRSKSILDEFLSQFDM-NSTTSKP 425
Query: 377 IILSAHDPVRYLGDVLAYVHSMIVNELEFLKSTFKLKSELV--TSDSVLKDNMDFIGDLH 434
IILSAHDP+RY+GDVLA VHS+I NE +F+KS F + E + T S+L+ N F+ +
Sbjct: 426 IILSAHDPIRYIGDVLASVHSIIANEADFVKSLFDFQDEDLKDTPISILQQNKTFLKGID 485
Query: 435 LKLLNEIFTSLANTIRIRLEQIVRFENNPMLNLDIVQCLSLYQMMLVKNGINESSQLMTS 494
KLLN+I SL+N+ RIR+EQIVRFE NP++N +IV+ L LY++M + GI + S ++ +
Sbjct: 486 NKLLNDIIQSLSNSCRIRIEQIVRFEENPIINFEIVRLLKLYRVMFERKGIQDDSSIINN 545
Query: 495 LNDLENLARSKIVSSVTSYLKD--LDKNQIAAADLLPPDWFVDYLSKLSQLLN------- 545
L LE++++++I+ Y+K + + + ++ DLLPP+W +Y++KL +L
Sbjct: 546 LKSLEDISKNRIIGYYEDYMKQTVMAETKNSSDDLLPPEWLSEYMNKLVELFEIYEKTHA 605
Query: 546 -KLEQQNETRILTDDLYDKLILDPINNNLVLNLQNWFPTAKKEKSARIDLLIVQINSFDL 604
+ E+ + ++L+ ++ PI + L+ LQ FP AKK + + LL ++IN FDL
Sbjct: 606 AEDEESEDNKLLSSKNLQTIVEQPIKDVLLKQLQTSFPLAKKNEKEKASLLTIEINCFDL 665
Query: 605 VKSKLGPFHSTIFSSDYGKSVFSKLETQYTLCVTKLKETMNSYLFESTGMELYFNLFNMI 664
+KS+L PF D + + + + ++ +LFE+TG++LY NL NMI
Sbjct: 666 IKSRLQPFEGLFAQDDDSRKITIWVCDKLKEYTKQMLTLQIKFLFENTGLDLYSNLVNMI 725
Query: 665 FPIISVQDELDYDMYLSAVENPIMKLAVIHDNIHEKLNTYLPLAVSDFQDVKLFNLMPPA 724
FP+ SV+DELDYDMYL+ +N +M+L ++ N+H+KLN YLP A++D Q LF L P
Sbjct: 726 FPVDSVKDELDYDMYLALRDNSLMELDMVRKNVHDKLNYYLPQALTDVQGNLLFKLTSPM 785
Query: 725 VEEDIVSTCFGNFIKFYKVFKSVLNKIYPEDEEMIMSTLNFTTNEVSTLLGVE 777
+ ++I CF FY +F+ +L +YP ++ + LNF+T+E L+G++
Sbjct: 786 IADEICDECFKKLSLFYNIFRKLLIHLYPNKKDQVFEILNFSTDEFDMLIGID 838
>Hs18582141 [S] KOG3758 Uncharacterized conserved protein
Length = 605
Score = 91.7 bits (226), Expect = 4e-18
Identities = 116/568 (20%), Positives = 232/568 (40%), Gaps = 80/568 (14%)
Query: 153 RKSLRSKLENSMLNSHSDILSNFQSIARRIKRLSGPLERINNAMQNF-------KDTTES 205
R++LR +E L + + +S F+ + ++ +S ++ ++N Q+ K+ T+
Sbjct: 20 RRNLRGDIERKSLAINEEFVSIFKEVKEELESISEDVQAMSNCCQDMTSRLQAAKEQTQD 79
Query: 206 IDFEFEHVKDRLDQLKARRQVIIKLRDSLTLTQLELDHLQN---GTINDLFFQILNKINF 262
+ + ++ +L+ R QV LT E+ L+ G I + FF+ L ++
Sbjct: 80 LIVKTTKLQSESQKLEIRAQVADAFLSKFQLTSDEMSLLRGTREGPITEDFFKALGRVKQ 139
Query: 263 IKEKATYLLSNEKTTSAGVALLKTMNNNLTVSNKRIYNYLINFIEEYDTLSRQYGERTIG 322
I LL + T AG+ +++ M + +R+Y + Q RT+
Sbjct: 140 IHNDVKVLLRTNQQT-AGLEIMEQMALLQETAYERLYRWA------------QSECRTLT 186
Query: 323 DES--LSNFQTSLIHLSNDVQFFQDF-LNRIVGLRSKRLLDDFLSQFDIDNKK-LQRPII 378
ES +S T + D + L+ R ++ F+ RPI
Sbjct: 187 QESCDVSPVLTQAMEALQDRPVLYKYTLDEFGTARRSTVVRGFIDALTRGGPGGTPRPIE 246
Query: 379 LSAHDPVRYLGDVLAYVHSMIVNELEFLKSTFKLKSELVTSDSVLKDNMDFIGDLHLKLL 438
+ +HDP+RY+GD+LA++H +E E L++ K VT+ V ++ + +G
Sbjct: 247 MHSHDPLRYVGDMLAWLHQATASEKEHLEALLK----HVTTQGVEENIQEVVG------- 295
Query: 439 NEIFTSLANTIRIRLEQIVRFENNPMLNLDIVQCLSLYQMMLVKNGINESSQLMTSLNDL 498
I + +++R+EQ++ E +L I L Y + N ++ L+T++ ++
Sbjct: 296 -HITEGVCRPLKVRIEQVIVAEPGAVLLYKISNLLKFYHHTISGIVGNSATALLTTIEEM 354
Query: 499 ENLARSKIVSSVTSYL-KDLDKNQIAAADLLPPDWFVDYLSKLSQLLNKLEQQNETRILT 557
L++ +S++ + K +DK ++ DL P L++ LL ++ +++ ++
Sbjct: 355 HLLSKKIFFNSLSLHASKLMDKVELPPPDLGPS----SALNQTLMLLREVLASHDSSVVP 410
Query: 558 DDLYD-------KLILDPINNNLVLNLQNWFPTAKKEKSARIDLLIVQINSFDLVKSKLG 610
D +LDP+ ++ N D+ +NS ++K+ L
Sbjct: 411 LDARQADFVQVLSCVLDPLLQMCTVSASN---------LGTADMATFMVNSLYMMKTTLA 461
Query: 611 PFHSTIFSSDYGKSVFSKLETQYTLCVTKLKETMNSYLFESTGMELYFNLFNMIFP---I 667
F ++ L+ Q + L SY+ G+ +N P
Sbjct: 462 LF-------EFTDRRLEMLQFQIEAHLDTLINEQASYVLTRVGLSYIYNTVQQHKPEQGS 514
Query: 668 ISVQDELD----------YDMYLSAVEN 685
++ LD +D YLSA +N
Sbjct: 515 LANMPNLDSVTLKAAMVQFDRYLSAPDN 542
>SPBC776.10c [S] KOG3758 Uncharacterized conserved protein
Length = 675
Score = 80.9 bits (198), Expect = 7e-15
Identities = 96/473 (20%), Positives = 200/473 (41%), Gaps = 41/473 (8%)
Query: 85 ISNNIEKPTTTNQLLSKKINSLLNVPNYASYTSDSETKRALQILDANHSDLGLNFNQLVA 144
I +N E+ +SK I L ++ NYA + ++ + N + V
Sbjct: 10 IKSNPEESLLFRNSVSKNIAQLSSI-NYADRNVQAALEQLSERKFKNEREARKQLPFEVF 68
Query: 145 SDFVGTLNRKSLRSKLENSMLNSHSDILSNFQSIARRIKRLSGPLERINNAMQNFKDTTE 204
SD + T S++ S++ S S+ ++ ++ ++ N + +
Sbjct: 69 SDLIWT----------NGSIIKELSELSSQTLSVQSQLLKVKNSIDSYKNEWSKKTNDAQ 118
Query: 205 SIDFEFEHVKDRLDQLKARRQVIIKLRDSLTLTQLELDHLQNGT-INDLFFQILNKINFI 263
+ +E + ++ + + I + + +L HL + T ++D F+ IL+K I
Sbjct: 119 ILLNSYETFCEEEALIEEKLKNIEIFEKNFVIMDDDLIHLTSSTDVDDRFYLILDKAQEI 178
Query: 264 KEKATYL---LSNEKTTSAGVALLKTMNNNLTVSNKRIYNYLINFIEEYDTLSRQYGERT 320
+ + L LS S+ ++K M+ + + ++++ + + + T
Sbjct: 179 HDSSDSLFASLSGFVEYSSFEEIVKKMSRYIEAAFEKLFRCVQTELSDPQTAQTL----- 233
Query: 321 IGDESLSNFQTSLIHLSNDVQFFQDFLNRIVGLRSKRLLDDFLSQFDI-DNKKLQRPIIL 379
E+ S+ + + L ++ +N IV R + L +L+ D RPI L
Sbjct: 234 ---EANSHLKKAFTKLFSEPTMVNKSINLIVQARQQILSTAYLTALTRGDFLSSSRPIEL 290
Query: 380 SAHDPVRYLGDVLAYVHSMIVNELEFLKSTFKLKSELVTSD--------SVLKDNMDFIG 431
SA D VR++GD+LA++H IVNE E +++ F + + + +VL+D M+ +
Sbjct: 291 SAPDTVRFIGDLLAWIHQTIVNEKELVEALFAARKRQIQLNPFPPWDVPNVLEDQMNSLL 350
Query: 432 DLHLKLLNEIFTSLANTIRIRLEQIVRFENNPMLNLDIVQCLSLYQMMLVKNGINESSQL 491
D L + +S A T + L IVR N +++ L Y+ K +++ +
Sbjct: 351 DGSLYGICRPLSSRAQTSVLDLSDIVRLYN-------VIEILGFYREAFSKI-VHDECII 402
Query: 492 MTSLNDLENLARSKIVSSVTSYLKDLDKNQIAAA-DLLPPDWFVDYLSKLSQL 543
+ + LE+ ++ + + L + ++ DLLPPD+ +L + +
Sbjct: 403 LRIIKTLEDFTYQRMKTVLDDELYTISNTNLSITDDLLPPDFVTTFLRNANSI 455
>At1g31780 [S] KOG3758 Uncharacterized conserved protein
Length = 706
Score = 80.9 bits (198), Expect = 7e-15
Identities = 96/454 (21%), Positives = 191/454 (41%), Gaps = 41/454 (9%)
Query: 153 RKSLRSKLENSMLNSHSDILSNFQS-------IARRIKRLSGPLERINNAMQNFKDTTES 205
R++LRS +E L +S+ L+ S + + L+ ++I A+ + TT
Sbjct: 47 RRNLRSTIEKRALQINSEFLNAADSTQIALDRVEEEVNALADCCDKIAAALSSSAATTSD 106
Query: 206 IDFEFEHVKDRLDQLKARRQVIIKLRDSLTLTQLELDHLQNGTINDLFFQILNKINFIKE 265
I E +K L+ R++++ L+ E+ L+ +N+ FFQ L+ + I
Sbjct: 107 IISTTERLKQELEVTTQRQEIVNCFLRDYQLSNEEIKALREDELNENFFQALSHVQEIHS 166
Query: 266 KATYLLSNEKTTSAGVALLKTMNNNLTVSNKRIYNYLINFIE--------------EYDT 311
LL AG+ L+ M + +R+ +I F + +Y
Sbjct: 167 NCKLLLRTHHQR-AGLELMDMMAVYQEGAYERLCRTIICFQKGSPFLNSFRANEKTQYWL 225
Query: 312 LSRQYGERTIGD----ESLSNFQTSLIHLSNDVQFFQDFLNRIVGLRSKRLLDDFLSQFD 367
L + R +GD E +T++ L F+ + LR L F+S
Sbjct: 226 LKQNAECRKLGDTDNPEVSELLRTAVRCLKERPVLFKYCAEEVGNLRHNALFRRFISALT 285
Query: 368 IDNKK-LQRPIILSAHDPVRYLGDVLAYVHSMIVNELEFLKSTFKLKSELVTSDSVLKDN 426
+ RPI + AHDP+RY+GD+L ++H + +E E + + F + S S K+
Sbjct: 286 RGGPGGMPRPIEVHAHDPLRYVGDMLGWLHQALASERELVHALFDIDS--ADHQSNAKNT 343
Query: 427 MDFI----GDLHLK-LLNEIFTSLANTIRIRLEQIVRFENNPMLNLDIVQCLSLYQMMLV 481
+ I G+ +L+ IF + ++R+EQ+++ + + +++ + L Y +
Sbjct: 344 SENIALKAGESDFTFVLDRIFEGVCRPFKVRVEQVLQSQPSLIISYKLTNTLEFYS-YTI 402
Query: 482 KNGINESSQLMTSLNDLENLARSKIVSSV-TSYLKDLDKNQIAAADLLPPDWFVDYLSKL 540
+ + + L ++ +++ A+ + T K L A DL PP + +S
Sbjct: 403 SDLLGRDTALCNTIGMVKDAAQKTFFDILKTRGEKLLRYPPPVAVDLSPPPAVREGVSLT 462
Query: 541 SQLLNKLEQQNETRILTDDLYDKLILDPINNNLV 574
+++ +N ++ +K DP+ + L+
Sbjct: 463 LEII-----ENYNSMMVSASGEKPAFDPVLSALL 491
>CE07351 [S] KOG3758 Uncharacterized conserved protein
Length = 642
Score = 74.3 bits (181), Expect = 7e-13
Identities = 91/470 (19%), Positives = 200/470 (42%), Gaps = 58/470 (12%)
Query: 154 KSLRSKLENSMLNSHSDILSNFQSIARRIKRLSGPLERINNAMQNFKDTTESIDFEF--- 210
+ L +KLE L + + LS F+ I +++ ++N+ N E++ F+
Sbjct: 54 RKLHNKLERYELRLNKEYLSEFEKINNIVQKFDELCVKMNSTCTNLSKQMETVKFKSVDL 113
Query: 211 ----EHVKDRLDQLKARRQVIIKLRDSLTLTQLELDHL----QNGTINDLFFQILNKINF 262
+K++ +++R +I + ++ +L+ EL L Q+G +++ FF++L + +
Sbjct: 114 VQKTASLKEKKASIESRCNMINEFLENYSLSSEELRELDECEQSGHLSEFFFKVLERCHE 173
Query: 263 IKEKATYLLSNEKTTSAGVALLKTMNNNLTVSNKRIYNYLINFIEEYDTLSRQYGERTIG 322
I+E ++ + +A + K ++R + + N L R++ T+
Sbjct: 174 IRENCRNMVQEQGHLAAFEVMEKMQK-----IDERSHAIICN------NLKREFQNLTVD 222
Query: 323 DESLSNFQTSLIHL-SNDVQFFQDFLNRIVGLRSKRLLDDFLSQFDIDNKKLQRPIILSA 381
+ + S + FQ +++ + RS+ LL+ F+ +I+ +Q P L+
Sbjct: 223 SHQKKQILSKAFKIISQNDAVFQLAIDQYISSRSQELLNQFV---EINKMAVQMPEGLA- 278
Query: 382 HDPVRYLGDVLAYVHSMIVNELEFLKSTFKLKSELVTSDSVLKDNMDFIGDLHLKLLNEI 441
+P++ +GD+L +H + E + S ++ + D LK
Sbjct: 279 -EPLKAVGDMLTSIHELTEQEKQLFSSICSAENLPIVLDECLK----------------- 320
Query: 442 FTSLANTIRIRLEQIVRFENNPMLNLDIVQCLSLYQMMLVKNGINESSQLMTSLNDLENL 501
SL + +IR+EQ++ E N + + L Y + I + S L DL
Sbjct: 321 --SLTSPFKIRVEQLLSTEKNAITIFKMGNILIFYANKF-ETLIRKDSYFTIMLADLVKT 377
Query: 502 ARSKIVSSVTSYLKDLDKNQIAA-ADLLPPDWFVDYLSKLSQLLNKLEQQNETRILTD-D 559
R ++ + ++ L + + DLLP LS L++ + + +L + +
Sbjct: 378 VRQVCIAGINHHVDGLMRKMTSPHYDLLPVPEVRQCLSLYHGLISIAVKTGDLNLLLEPE 437
Query: 560 LYDKLILDPINNNLVLNLQNWFPTAKKEKSARIDLLIVQINSFDLVKSKL 609
+ +L+P+ + L +A + KS I++ + IN +++S +
Sbjct: 438 RIYEYVLEPLIQTVQL-------SATRLKS-DIEVSVFTINCLTVIRSAI 479
>7303912 [S] KOG3758 Uncharacterized conserved protein
Length = 630
Score = 73.9 bits (180), Expect = 9e-13
Identities = 100/481 (20%), Positives = 202/481 (41%), Gaps = 63/481 (13%)
Query: 85 ISNNIEKPTTTNQLLSKKINSLLNVPNYASYTSDSETKRALQILDANHSDLGLNFNQLVA 144
+S+ EKP +++ K++N +L A SD +T AL L ++ L
Sbjct: 1 MSSTQEKPEELDRI-QKRVNKILE----ARLESDKDTLDALSGLSTFFTENTLQ------ 49
Query: 145 SDFVGTLNRKSLRSKLENSMLNSHSDILSNFQ-------SIARRIKRLSGPLERINNAMQ 197
NR++LRS++E+ + + + L F+ ++ + ++ +E + + ++
Sbjct: 50 -------NRRNLRSQIEHRSVGINENFLKAFRQVKLSLDAVCEDLDTMATSVETMKSDLE 102
Query: 198 NFKDTTESIDFEFEHVKDRLDQLKARRQVIIKLRDSLTLTQLELDHLQNGTINDL----- 252
K T+ + + ++ D+L+ +Q+ L+ E L G D
Sbjct: 103 TSKALTKDLIKQTNTMQRERDRLEVHKQIAQAFLARFQLSGAE-HQLLYGAAKDAPIVAD 161
Query: 253 FFQILNKINFIKEKATYLLSNEKTTSAGVALLKTMNNNLTVSNKRIYNYLINFIEEYDTL 312
FF++L+++ I L+ T+A + +++ M + + +R+Y + N +
Sbjct: 162 FFRVLDRVQSIHADCRLLMQCGYQTAA-IDIMEEMTLHQEGALERLYRWTQNHCRSLE-- 218
Query: 313 SRQYGERTIGDESLSNFQTSLIHLSNDVQFFQDFLNRIVGLRSKRLLDDFLSQFDIDNKK 372
+ + G + E++S Q + F+ ++ R L+ F+
Sbjct: 219 NNEIGPLIV--EAMSRLQDRPV-------LFKYVIDEYAIARRAVLVRQFIEALTEGGPG 269
Query: 373 LQ-RPIILSAHDPVRYLGDVLAYVHSMIVNELEFLKSTFKLKSELVTSDSVLKDNMDFIG 431
+PI L AHDP RY+GD+ A++H I E E L FK + SD L++ + +I
Sbjct: 270 GNPKPIELHAHDPKRYIGDMFAWLHQSIPTEKENLSLLFKKCDKQDISDQ-LQNALGYIA 328
Query: 432 DLHLKLLNEIFTSLANTIRIRLEQIVRFENNPMLNLDIVQCLSLYQMML--VKNGINESS 489
D + + +++R+E I++ E + ++ I L YQ ++ V G +
Sbjct: 329 D-----------GVCHPLKVRVETILQAEKDTIVLFTISNLLRFYQQIMRQVVQGGSLEE 377
Query: 490 QLMTSLNDLENLARSKIVSSVTSYLKDLDKNQIAA-----ADLLPPDWFVDYLSKLSQLL 544
L+ E + + + V S L+ A DL+PP L+ L ++L
Sbjct: 378 CLVELQKSSEQIYLGALAAQVRSVLQRPSGGSGLALEPPQRDLVPPPSVARLLNMLKEIL 437
Query: 545 N 545
+
Sbjct: 438 S 438
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.135 0.369
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 41,389,239
Number of Sequences: 60738
Number of extensions: 1704951
Number of successful extensions: 6034
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 6013
Number of HSP's gapped (non-prelim): 8
length of query: 779
length of database: 30,389,216
effective HSP length: 114
effective length of query: 665
effective length of database: 23,465,084
effective search space: 15604280860
effective search space used: 15604280860
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)