ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactVI1125   suspect: LH   E     KOG1303  Amino acid transport and metabolism                            Amino acid transporters

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactVI1125 392229  393725 499  
         (499 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CE21420 [E] KOG1303 Amino acid transporters                            54   5e-07
CE21306 [E] KOG1303 Amino acid transporters                            52   2e-06

>CE21420 [E] KOG1303 Amino acid transporters
          Length = 503

 Score = 53.9 bits (128), Expect = 5e-07
 Identities = 67/297 (22%), Positives = 126/297 (41%), Gaps = 33/297 (11%)

Query: 74  SWQHTTGLLLSEYIVLAIMSFPWSYSVLGLIPGLILTAFVAITVLYTGLIVLDYCE---- 129
           SW      ++ + +   ++S P +    GLI G +L    +I   YTG+ + +  E    
Sbjct: 40  SWLMAAVFIVGDMMGAGMISLPLALGRSGLIAGCVLILLASIFSGYTGIQLGENWEMMQI 99

Query: 130 RFPHLKNVCDIG-QHLFWGSKAAW--YATAVCFIANNTLIQGLHVLVGAK----YLNTVT 182
           R+P  +  C      + + +   W     AVC + +  LI  + +L+ A+     LNT  
Sbjct: 100 RWPKYRTHCRRPYPEMAYRALGNWARQVVAVCLVVSQFLIACVLLLISAENFTNLLNTFF 159

Query: 183 NHSVCSVGFAAIVAVISFVFSIPRTFSSLSKVAYFSAITMFISVILAMIFAGIQDHPAGY 242
           +  +    F   +A++ + FS+ ++     ++A  SA++       + I AG+    A +
Sbjct: 160 HLHLDFCVFIVAIALVLWPFSLLQSPMDFWQLAVISAVS-------STIAAGLIVFGASW 212

Query: 243 DGTA-VTYQLWPKKGTTYVDAMGAFLNIVYTFVGQITYPQFISEMKRPREFKKVL---WI 298
           D T+ V Y+  P           A+  IV+ F G   +P    +M  P +F K +   +I
Sbjct: 213 DMTSCVPYRQMPSLEAKQFTL--AYGTIVFAFGGHGAFPTIQHDMAMPHQFNKSVISSYI 270

Query: 299 VTALELIIFSLAGALIYVYVGNEYMTAPAYGSL-----TKTYKIISFSFAIPTIVFV 350
           +  L  +  S+ G + Y     + M      S+      +T  I+  +  +PTI+ V
Sbjct: 271 LITLVYLAVSITGLIAY----GDSMIDTVIPSIQLTWVAQTINILITAHILPTIIIV 323


>CE21306 [E] KOG1303 Amino acid transporters
          Length = 484

 Score = 52.4 bits (124), Expect = 2e-06
 Identities = 76/340 (22%), Positives = 149/340 (43%), Gaps = 41/340 (12%)

Query: 91  IMSFPWSYSVLGLIPGLILTAFVAITVLYTGLIVLDYC----ERFPHLKNVCD-----IG 141
           +++ P +    G + G  L    A+   YTG+++ +      E +P  ++ C      +G
Sbjct: 29  LIALPTAIVSTGAVTGAFLLLVAAVVCTYTGILLAENWTILQELYPEYRDHCRKPYPAMG 88

Query: 142 QHLFWGSKAAWYATAVCFIAN-NTLIQGLHVLVGAK------YLNTVTNHSVCSVGFAAI 194
                G K A + +A+  +    T +  + VL+ AK      + N  T+ S C +    I
Sbjct: 89  LRAI-GPKFAHFVSAMLQVTQFGTAV--VFVLLAAKNGENMLHANFGTHVSFCYM--ILI 143

Query: 195 VAVISFVFSIPRTFSSLSKVAYFSAITMFISVILAMIFAGIQDHPAGYDGTAVTYQLWPK 254
           V ++ F F++P++          + I+  ISV+L +IF  +QD+   +    V Y  +  
Sbjct: 144 VGLLVFPFTLPKSPKDFWYAVVAAMISTTISVVL-IIFGTVQDYEVCH--KEVFYPSF-- 198

Query: 255 KGTTYVDAMGAFLNIVYTFVGQITYPQFISEMKRPREFKKVLWIVTALELIIFSLAGALI 314
               +   + +F  I++++ G   +P    +MK+P  F + + I+  + + IF L  ++ 
Sbjct: 199 ---NFSKTLMSFGTIMFSYGGHCAFPTIQHDMKKPHHFSRSI-IIAFIIIFIFYLPVSMS 254

Query: 315 YVYVGNEYMTAPAYGSLTKTYKIISFSFAIPTIV-FVGSLYSNISSRFVFFNVFRNSEHL 373
             +V    +T     S+         +  I TIV  + SL+ +++    F  + +  E +
Sbjct: 255 GYFVYGSSLTDSIIPSIQ--------NINIQTIVNILISLHVSLALTITFNPLNQEFEEI 306

Query: 374 YSHT-KVGWLAWIGLLLLTWVGAFVIAEVIPFFSDLLSLM 412
            + +   GW   +   L+     FV AE +P F  LL L+
Sbjct: 307 LNMSHDFGWQRIVARALVMLSVVFV-AESVPNFGVLLDLV 345


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.325    0.139    0.431 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,271,796
Number of Sequences: 60738
Number of extensions: 1157700
Number of successful extensions: 3657
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 3656
Number of HSP's gapped (non-prelim): 2
length of query: 499
length of database: 30,389,216
effective HSP length: 111
effective length of query: 388
effective length of database: 23,647,298
effective search space: 9175151624
effective search space used: 9175151624
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)