ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI1198 suspect: LH U KOG2115 Intracellular trafficking, secretion, and vesicular transport Vacuolar sorting protein VPS45
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI1198 416114 418651 846
(846 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR027c [U] KOG2115 Vacuolar sorting protein VPS45 612 e-175
SPAC2F3.10 [U] KOG2115 Vacuolar sorting protein VPS45 96 3e-19
>YDR027c [U] KOG2115 Vacuolar sorting protein VPS45
Length = 889
Score = 612 bits (1578), Expect = e-175
Identities = 337/795 (42%), Positives = 495/795 (61%), Gaps = 30/795 (3%)
Query: 79 TQKHGLDYSPLVDNSIYEIVMNTRFKRWLRHPTTEDIPPVVLSXXXXXXXXXXXXXQYRD 138
T +D+SPL +NSIYEIVMNTR K WL +PT DIP V LS +Y
Sbjct: 98 THSGSMDFSPLGNNSIYEIVMNTRRKNWLNYPTVADIPQVSLSKNDLDDHWKTHVIEYVK 157
Query: 139 DIKDEYRVYQSTNNISKLNRMEQMRSID-----HDKT-ALGLQNTKAKAFEEIPEFYFDK 192
+IK +Y+++QSTNNI +N+MEQ++ + H+++ L+ A+ IP+FYF
Sbjct: 158 NIKSDYQIFQSTNNIRNMNQMEQLKELREGENMHEESFEANLRQGDAELINSIPDFYFSD 217
Query: 193 EFKLDHPNFFRMLLRDFEFDANQLLNDDDKVRNDSYEELQGRLAFYLDTVEGLLVSEISK 252
+F+LD+P F +L + +L R++++ EL+ RL +LD VE LLV+EISK
Sbjct: 218 KFQLDNPRTFHKVLDAIDLFLTKLDMKRQAERDEAFSELRDRLNDFLDIVETLLVTEISK 277
Query: 253 SIQSFFDALEDVDKIRENATETINELDSAAEALERINGDKISKRIEMIKDLIRRKNVEKL 312
S FF AL +VD I++ A +T++EL A+ ++ I+ + I K+I ++ + +RKNVEKL
Sbjct: 278 SSHKFFHALSEVDNIQKRALDTMSELKELAQNIKTIDAENIRKKISHLEMIFKRKNVEKL 337
Query: 313 QQGILQVKLAXXXXXXXXXXXXXXXLQECLKLVKAVENLINGDND-DPTVNDWTSQWPYK 371
+QG+LQ KL L CL+L+K+++ LI GD+ + V WT WPYK
Sbjct: 338 EQGLLQAKLVLNKTDECKSMYEENKLDNCLELIKSIDYLIKGDDSINEDVQSWTRCWPYK 397
Query: 372 LTNISSFSSLQGIIDMLNNLKVDIGGSYSLSLVTVLLEDLRTYSKSQSKNDTIIKLQSRK 431
L+N+ + +L + L N+K++IGG +SL L +L++DLR++ KS +T+ ++Q+
Sbjct: 398 LSNLRTIPALSATREFLTNMKIEIGGKFSLQLSILLIDDLRSFCKSIKPKETLHRIQTGS 457
Query: 432 DKNKPHLKIDETFRKQIKENLIQLIDCDQLVPAVKLYEDKFIAELKSVIKVNLPHERQTP 491
+ K + D F +I E +++L C++L A LY +K I ELKS+IK+ LP E
Sbjct: 458 NDKKQTIFTDN-FSSKITELIVRLNRCEELTSAFDLYREKSITELKSIIKIYLPTENAHA 516
Query: 492 EESKDGSKPT--AANGSRLSKLIQEQSSQEFQSMLVNIFTSEIELLKRLTRHQKMLLDIS 549
+ + D + +GS+LS+LI+EQ+ EFQSMLVNIFT +E L+RL HQK+LLDIS
Sbjct: 517 DNNHDEKHLNNGSTSGSKLSRLIKEQTPAEFQSMLVNIFTHALEALRRLYGHQKLLLDIS 576
Query: 550 LSELPTTA---EQSDKMIIDLDIRKSVNEGIRIVQLRMGKIISVRRDITCTLRFDHFLQF 606
L+EL + E MI LDIR +NE IRI+QLR GKII+VRR++ +LR+D+FL+F
Sbjct: 577 LNELASVKSPNENQHNMITQLDIRTGINEIIRIIQLRTGKIIAVRRELNLSLRYDYFLKF 636
Query: 607 YEICMLFMRECESISGEFLTKYLSDVLNLQIDTYLKSLQNLNIRVLKQKIDVERWTPFIV 666
Y IC++F++ECE +SGEFLTKYLS+VL QI Y + + N R +K+KID E W P+IV
Sbjct: 637 YAICVIFIQECEVLSGEFLTKYLSNVLASQIKHYANAQSSKNYRNIKKKIDAEEWIPYIV 696
Query: 667 EPELQKDVNDIVSCIDIDPSNWTRFIEL-------NKPSPLDETKDE-KQSSTGHKKSVV 718
+ +Q DVNDIVS IDIDP +WT +++ D+ KDE ++ GH+KSVV
Sbjct: 697 DSSIQSDVNDIVSSIDIDPLSWTTILDMVGGSHDCENGRSEDKEKDEGNETYQGHRKSVV 756
Query: 719 LGDKTFVASEALILAIKMIKTLLVLASNFPSQYARHCERQLSDVLKFFNTKAIESISSSL 778
+GDKTFVAS +L+ I++IK L+VL+ N PS Y + E+ D L+++N+ A+ S++
Sbjct: 757 VGDKTFVASSSLLATIEVIKELMVLSINLPSIYLSNFEKLCYDALQYYNSSAMASVTQPG 816
Query: 779 TDRLSTKTDKNLNIMAESLDCLAEITLLLQGYFQRTLGSKTD-------KYENIWKQLQQ 831
L KT +NL+IM ESLDCLAE +++Q ++QR S D Y + Q Q
Sbjct: 817 NSLL--KTGRNLSIMGESLDCLAEFVIIVQRFYQRLSNSNRDFEPFDASHYTTLLGQFQA 874
Query: 832 SSEKLFQTNNIPPPV 846
SS K++ N PPPV
Sbjct: 875 SSNKIYMANAPPPPV 889
>SPAC2F3.10 [U] KOG2115 Vacuolar sorting protein VPS45
Length = 949
Score = 95.5 bits (236), Expect = 3e-19
Identities = 138/684 (20%), Positives = 268/684 (39%), Gaps = 76/684 (11%)
Query: 181 AFEEIPEFYFDKEFKLDHPNFFRMLLRDFEFDANQLLNDDDKVRNDSYEELQGRLAFYLD 240
+ +P +F +F LD P F ++ + + ++ + LQ ++++YLD
Sbjct: 165 SISSVPSVFFQSDFNLDDPQIFDVVSEHIDITQTSDAPNSNRNLLLNNSMLQEKISWYLD 224
Query: 241 TVEGLLVSEISKSIQSFFDALEDVDKIRENATETINELDSAAEALERING------DKIS 294
TVE L+ EI + SF ++++ ++++ + + E E L +N D IS
Sbjct: 225 TVELHLLQEIENASDSFPMIIDNLKQLKKETRDNVEETKHLLEKLTEVNVMCDRHMDAIS 284
Query: 295 KRIEMIKDLIRRKNVEKLQQGILQVKLAXXXXXXXXXXXXXXXLQECLKLVKAVENLING 354
L + K V + + I + E +K + +E ING
Sbjct: 285 SEELACHQLTQLKKVVQTLEDIYS---------------EHKKVSEDVKDQRFLE-AING 328
Query: 355 DNDDPTVNDWTSQWPYKLTNIS----SFSSLQGIIDMLNNLKVDIGGSYSLSLVTVLLED 410
+N +Q K T ++ S S++ + + L + S L D
Sbjct: 329 ------INSIVTQLKKKSTELNVDLLSLPSVKSLREEHKVLYHTVSQSVVQQFSKFLTSD 382
Query: 411 LRTYSKSQSKND------TIIKLQSRKDKNKPH-LKIDETFRKQIKENLIQLIDCDQLVP 463
+ +S N+ + +L K+ P ++ D F ++ + L D L
Sbjct: 383 MHKFSTILDSNELKDIYLSKNRLIPSVTKSLPSAMEFDADFLHEVDVCIDCLTLTDSLQA 442
Query: 464 AVKLYEDKFI--------------AELKSVIKVNLPHERQTPEESKDGSKPTAANGSRLS 509
A+ LY+ E++SV K + R S ++ S +
Sbjct: 443 ALTLYKTAVFEAFENLAQQYFTDGVEMRSVRKSS-DLRRSLSAASLSSLSDSSRRSSSAA 501
Query: 510 KLIQEQSSQEFQSMLVNIFTSEIELLKRLTRHQKMLLDISLSELPTTAEQSDKMIIDLDI 569
Q S EF ML NI+ S +E L+R+ K+L+DI LS+ T ++ DL
Sbjct: 502 NPFQSMSITEFAEMLTNIYFSSLECLRRIRSQIKVLIDI-LSKKDTKQYHLSVILNDLMA 560
Query: 570 RKSVNEGIRIVQLRMGKIISVRRDITCTLRFDHFLQFYEICMLFMRECESISGEFLTKYL 629
S +V ++ I ++R + T ++ + + + ++F E ES+ G + ++
Sbjct: 561 TSS-----DVVTQQVTTINNLRFRVLDTYPPNNVIYLFSLHIIFHNELESLCGITQSSFI 615
Query: 630 SDVLNLQIDTYLKSLQNLNIRVLKQKIDVERWTPFIVEPELQKDVNDIVSCIDIDPSNWT 689
++ +D + K+ Q + + L + E W VEP Q N + C P WT
Sbjct: 616 PTIMRQLLD-WFKNFQRYSTQKLASSFERELWEAIPVEPSCQDLANRVFECSGNTPVEWT 674
Query: 690 RFIELNKPSPLDETKDEK------QSSTGHKKSVVLGDKTFVASEALILAIKMIKTLLVL 743
R + SP E +DE + S +V G ++ + + ++ + L
Sbjct: 675 RLL-----SPEAEKEDENHRVHHVKVSEDCTATVSFGKQSLHIVRSSVTLLETLDDYGKL 729
Query: 744 ASNFPSQYARHCERQLSDVLKFFNTKAIESISSSLTDR---LSTKTDKNLNIMAESLDCL 800
++F S+ A + + D+ + N++ + I + T R LS K++ + ++++
Sbjct: 730 LAHF-SRLAPEFQTGMLDMFRTLNSRVYQLILGAGTVRSSGLSKVLGKHIALASQTIALF 788
Query: 801 AEITLLLQGYFQRTLGSKTDKYEN 824
+ Y R G++ N
Sbjct: 789 QLSLNSMCRYLSRVTGTRLTNETN 812
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.134 0.372
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,740,395
Number of Sequences: 60738
Number of extensions: 1721281
Number of successful extensions: 6298
Number of sequences better than 1.0e-05: 2
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 6288
Number of HSP's gapped (non-prelim): 4
length of query: 846
length of database: 30,389,216
effective HSP length: 115
effective length of query: 731
effective length of database: 23,404,346
effective search space: 17108576926
effective search space used: 17108576926
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)