ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI1320 good L KOG2299 Replication, recombination and repair Ribonuclease HI
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI1320 466276 465365 -304
(304 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YNL072w [L] KOG2299 Ribonuclease HI 347 2e-95
SPAC4G9.02 [L] KOG2299 Ribonuclease HI 233 3e-61
7296208 [L] KOG2299 Ribonuclease HI 178 8e-45
At2g25100 [L] KOG2299 Ribonuclease HI 170 3e-42
Hs5454010 [L] KOG2299 Ribonuclease HI 169 3e-42
CE28960 [L] KOG2299 Ribonuclease HI 157 2e-38
ECU03g0670 [L] KOG2299 Ribonuclease HI 138 1e-32
>YNL072w [L] KOG2299 Ribonuclease HI
Length = 307
Score = 347 bits (889), Expect = 2e-95
Identities = 180/306 (58%), Positives = 221/306 (71%), Gaps = 17/306 (5%)
Query: 16 VPPTVAGSLNSLHSKTYHSEVPKSILESTDE-IILGVDEAGRGPVMGPMVYGISYCTRQY 74
VPPTV SL S ++K+Y S VP ++LE D II+G+DEAGRGPV+GPMVY ++Y T++Y
Sbjct: 2 VPPTVEASLESPYTKSYFSPVPSALLEQNDSPIIMGIDEAGRGPVLGPMVYAVAYSTQKY 61
Query: 75 EKD-VICKYGFDDSKKLTDPVRQKLFAMIY--GGEIDGVGYATTSITACDISSGMFRFPP 131
+ + +I Y FDDSKKLTDP+R+ LF+ IY E+ +GYATT IT DIS GM +FPP
Sbjct: 62 QDETIIPNYEFDDSKKLTDPIRRMLFSKIYQDNEELTQIGYATTCITPLDISRGMSKFPP 121
Query: 132 EKNYNLNEQAHDVTIALIDDVLKSGVKVGHVYVDTVGPPISYQKKLESIFPQCKFTVAKK 191
+NYNLNEQAHDVT+ALID V+K VK+ HVYVDTVGPP SYQKKLE FP KFTVAKK
Sbjct: 122 TRNYNLNEQAHDVTMALIDGVIKQNVKLSHVYVDTVGPPASYQKKLEQRFPGVKFTVAKK 181
Query: 192 ADSIYPIVSVASVVAKVTRDIILSQCKKTEDEILGSGYPGDSKTVQWLRNEMKPLMGWSP 251
ADS+Y +VSVASVVAKVTRDI++ K+ DEILGSGYP D KTV WL+ LMGW
Sbjct: 182 ADSLYCMVSVASVVAKVTRDILVESLKRDPDEILGSGYPSDPKTVAWLKRNQTSLMGWPA 241
Query: 252 YMVRFSWQTCATMLAN-SKSTIEIEWEEDAMTKKK------------MQWAPEQKNRPIT 298
MVRFSWQTC T+L + SK++I I+WEE M +K M P ++ R T
Sbjct: 242 NMVRFSWQTCQTLLDDASKNSIPIKWEEQYMDSRKNAAQKTKQLQLQMVAKPVRRKRLRT 301
Query: 299 LDSWYQ 304
LD+WY+
Sbjct: 302 LDNWYR 307
>SPAC4G9.02 [L] KOG2299 Ribonuclease HI
Length = 326
Score = 233 bits (593), Expect = 3e-61
Identities = 134/295 (45%), Positives = 188/295 (63%), Gaps = 16/295 (5%)
Query: 16 VPPTVAGSLNSLHSKTYHSEVPKSILESTDEIILGVDEAGRGPVMGPMVYGISYCTRQYE 75
+PP+V S N S YHS V I +S LGVDEAGRGPV+GPMVY ++YC ++
Sbjct: 35 LPPSVNKS-NPAKSNYYHSTVTDDISKS-QPYRLGVDEAGRGPVLGPMVYAVAYCPVDFD 92
Query: 76 KDVICKYGFDDSKKLTDPVRQKLFAMIYGGEID---GVGYATTSITACDISSGMFRFPPE 132
+ YGF DSK L R++L +I + VG++T SI+A ++++GM R+
Sbjct: 93 ---LTNYGFADSKTLASLKREELLKLICNKSNELGKNVGWSTMSISARELAAGMLRY--R 147
Query: 133 KNYNLNEQAHDVTIALIDDVLKSGVKVGHVYVDTVGPPISYQKKLESIFPQCKFTVAKKA 192
YNLN QAHD TI LI V +SG+ V +YVDTVGPPISYQ+KL++ FPQ K TV KKA
Sbjct: 148 NKYNLNLQAHDTTIDLIKKVYESGINVTEIYVDTVGPPISYQEKLQAHFPQAKVTVTKKA 207
Query: 193 DSIYPIVSVASVVAKVTRDIILSQCKKT-EDEILGSGYPGDSKTVQWLRNEMKPLMGWSP 251
DS++PIVS+AS+ AKVTRDI L +++ E GSGY D++T +WL+ + + GW
Sbjct: 208 DSLFPIVSLASICAKVTRDIQLECARESIRTENWGSGYSSDARTTEWLKVNVDKIFGWKG 267
Query: 252 YMVRFSWQTCATML---ANSKSTIEIEWEEDAMTKKKMQWAPEQKNRPITLDSWY 303
+VR+SW+T +L + S+S+IEI+W ED T + + ++K P + SW+
Sbjct: 268 DIVRYSWKTAKDLLELPSKSQSSIEIDWHEDDDT-PTLNFTQKKKPNPAS-RSWF 320
>7296208 [L] KOG2299 Ribonuclease HI
Length = 347
Score = 178 bits (452), Expect = 8e-45
Identities = 120/275 (43%), Positives = 155/275 (55%), Gaps = 17/275 (6%)
Query: 18 PTVAGSLNSLHSKTYHSEVPKSILESTDEIILGVDEAGRGPVMGPMVYGISYCTRQYEKD 77
P +AG NS ++ Y S+VP + +LGVDEAGRGPV+GPMVYGISYC + K
Sbjct: 45 PFIAGKENSRNT-VYLSDVPDICKDKP--CMLGVDEAGRGPVLGPMVYGISYCPLESNK- 100
Query: 78 VICKYGFDDSKKLTDPVRQKLFAMIYGGEI--DGVGYATTSITACDISSGMFRFPPEKNY 135
+ G DSK+LT+ R +F I E VG+A I+ IS+ M+R
Sbjct: 101 ALEDLGCADSKQLTEGKRDIIFNDINTKEYATSCVGWAVEIISPNTISTSMYR---RSKC 157
Query: 136 NLNEQAHDVTIALIDDVLKSGVKVGHVYVDTVGPPISYQKKLESIFPQCKFTVAKKADSI 195
+LNE + D + LI + +GV + VYVDTVGPP YQ+KL FP K TVAKKADS
Sbjct: 158 SLNEVSMDSAMGLIQQAIDAGVNIAEVYVDTVGPPEKYQEKLLKRFPSFKITVAKKADST 217
Query: 196 YPIVSVASVVAKVTRDIILSQCKKTE-----DEILGSGYPGDSKTVQWLRNEMKPLMGWS 250
YPIVS AS+ AKVTRD L E D GSGYPGD T ++L + + G+
Sbjct: 218 YPIVSAASICAKVTRDHALKVWSFPEGLVIKDNEFGSGYPGDPVTRRFLTEYIDLVFGF- 276
Query: 251 PYMVRFSWQTCATMLANSKSTIEIEWEEDAMTKKK 285
P +VRFSW T LA+ ++E++E K K
Sbjct: 277 PRLVRFSWSTAENALAD--KAYDMEFDEPDSEKPK 309
>At2g25100 [L] KOG2299 Ribonuclease HI
Length = 253
Score = 170 bits (430), Expect = 3e-42
Identities = 101/235 (42%), Positives = 142/235 (59%), Gaps = 17/235 (7%)
Query: 48 ILGVDEAGRGPVMGPMVYGISYCTRQYEKDVICKYGFDDSKKLTDPVRQKLFAMIYGGEI 107
++G+DEAGRGPV+GPMVYG YC Y+ + + F DSK L + R++L+ + ++
Sbjct: 16 LMGIDEAGRGPVLGPMVYGCMYCPISYQSSLASLH-FADSKTLKEEKREELYESL---KL 71
Query: 108 D-GVGYATTSITACDISSGMFRFPPEKNYNLNEQAHDVTIALIDDVLKSGVKVGHVYVDT 166
D +G+A I ++S+ M + NLNE +H+ + LI VL GV + Y+DT
Sbjct: 72 DKSLGWAVDVIDPRELSAKMLA---KNKTNLNEISHNSAMGLIKRVLDMGVLLTEAYLDT 128
Query: 167 VGPPISYQKKLESIFPQCKFTVAKKADSIYPIVSVASVVAKVTRDIILSQ--CKKTEDEI 224
VG P Y+ KL FP KF V+KKADS++PIVS AS+VAKVTRD L + ++T ++I
Sbjct: 129 VGDPDKYRIKLSERFPSIKFVVSKKADSLFPIVSGASIVAKVTRDRALKEWLVEETGEDI 188
Query: 225 ---LGSGYPGDSKTVQWLRNEMKPLMGWSPYMVRFSWQTCATMLANSKSTIEIEW 276
GSGYPGD +T WL + G+ P +VRFSW TC T L K +E+ W
Sbjct: 189 NRNFGSGYPGDPETKAWLVQHKHSVFGF-PSLVRFSWGTCTTHL---KGEVEVAW 239
>Hs5454010 [L] KOG2299 Ribonuclease HI
Length = 299
Score = 169 bits (429), Expect = 3e-42
Identities = 102/238 (42%), Positives = 137/238 (56%), Gaps = 14/238 (5%)
Query: 48 ILGVDEAGRGPVMGPMVYGISYCTRQYEKDVICKYGFDDSKKLTDPVRQKLFAMIYGGEI 107
+LGVDEAGRGPV+GPMVY I YC D+ DSK L + R++LFA + +
Sbjct: 30 VLGVDEAGRGPVLGPMVYAICYCPLPRLADLEA-LKVADSKTLLESERERLFAKME--DT 86
Query: 108 DGVGYATTSITACDISSGMFRFPPEKNYNLNEQAHDVTIALIDDVLKSGVKVGHVYVDTV 167
D VG+A ++ IS+ M YNLN +HD LI L GV V V+VDTV
Sbjct: 87 DFVGWALDVLSPNLISTSMLG---RVKYNLNSLSHDTATGLIQYALDQGVNVTQVFVDTV 143
Query: 168 GPPISYQKKLESIFPQCKFTVAKKADSIYPIVSVASVVAKVTRDIILSQCKKTE-----D 222
G P +YQ +L+ FP + TV KAD++YP+VS AS+ AKV RD + + + E D
Sbjct: 144 GMPETYQAQLQQSFPGIEVTVKAKADALYPVVSAASICAKVARDQAVKKWQFVEKLQDLD 203
Query: 223 EILGSGYPGDSKTVQWLRNEMKPLMGWSPYMVRFSWQTCATMLANSKSTIEIEWEEDA 280
GSGYP D KT WL+ ++P+ G+ P VRFSW+T T+L K ++ WE+ A
Sbjct: 204 TDYGSGYPNDPKTKAWLKEHVEPVFGF-PQFVRFSWRTAQTIL--EKEAEDVIWEDSA 258
>CE28960 [L] KOG2299 Ribonuclease HI
Length = 297
Score = 157 bits (396), Expect = 2e-38
Identities = 97/254 (38%), Positives = 141/254 (55%), Gaps = 20/254 (7%)
Query: 48 ILGVDEAGRGPVMGPMVYGISYCTRQYEKDVICKYGFDDSKKLTDPVRQKLFAMIYGGEI 107
+LG+DEAGRGPV+GPMVY + + + G DDSK L + R+++F + E
Sbjct: 23 VLGIDEAGRGPVLGPMVYAAAISPLDQNVE-LKNLGVDDSKALNEAKREEIFNKMNEDED 81
Query: 108 --DGVGYATTSITACDISSGMFRFPPEKNYNLNEQAHDVTIALIDDVLKSGVKVGHVYVD 165
+ YA ++ IS M + + Y+LNE +H+ I LI D L V V + VD
Sbjct: 82 IQQIIAYALRCLSPELISCSMLK---RQKYSLNEVSHEAAITLIRDALACNVNVVEIKVD 138
Query: 166 TVGPPISYQKKLESIFPQCKFTVAKKADSIYPIVSVASVVAKVTRDIILSQCK------K 219
TVGP +YQ KLE +FP V +KADS++PIVS AS+ AKVTRD L + K
Sbjct: 139 TVGPKATYQAKLEKLFPGISICVTEKADSLFPIVSAASIAAKVTRDSRLRNWQFREKNIK 198
Query: 220 TEDEILGSGYPGDSKTVQWLRNEMKPLMGWSPYMVRFSWQTCATMLANSKSTIEIEWEED 279
D GSGYPGD T ++L+ ++P+ G+ +VR SW+T +T++ K + WE+D
Sbjct: 199 VPDAGYGSGYPGDPNTKKFLQLSVEPVFGFCS-LVRSSWKTASTIV--EKRCVPGSWEDD 255
Query: 280 -----AMTKKKMQW 288
+ +K+ W
Sbjct: 256 EEEGKSQSKRMTSW 269
>ECU03g0670 [L] KOG2299 Ribonuclease HI
Length = 239
Score = 138 bits (347), Expect = 1e-32
Identities = 87/226 (38%), Positives = 128/226 (56%), Gaps = 17/226 (7%)
Query: 45 DEIILGVDEAGRGPVMGPMVYGISYCTRQYEKDVICKYGFDDSKKLTDPVRQKLFAMIYG 104
+E+++G+DEAGRGPV+G MVYG R +DV GF DSK LT R F MI
Sbjct: 16 EEVVVGIDEAGRGPVVGYMVYGALVVPRGVLEDV----GFKDSKVLTPAQRYNFFQMI-- 69
Query: 105 GEIDGVGYATTSITACDISSGMFRFPPEKNYNLNEQAHDVTIALIDDVLKSGVKVGHVYV 164
+ G GYA C + ++ +LNE + + ++D+V +V +YV
Sbjct: 70 -KKKGFGYA----YHCSHPDYITEMMQVRSVSLNEVSIGSVLRILDEVQSKCRRVDTIYV 124
Query: 165 DTVGPPISYQKKLESIFPQCKFTVAKKADSIYPIVSVASVVAKVTRDIILSQCKKTEDEI 224
D +G Y+++LE +P +F V ++ADS + +VS AS+VAKV RD+++S+ E
Sbjct: 125 DALGDCRKYKERLEKDYPY-RFVVEERADSKFQVVSGASIVAKVQRDMLISEF----GED 179
Query: 225 LGSGYPGDSKTVQWLRNEMKPLMGWSPYMVRFSWQTCATMLANSKS 270
LGSGYP D +T++WLR + + G+ P VR+SW T ML KS
Sbjct: 180 LGSGYPSDPETIRWLRKNINVVFGFPP-GVRYSWATVKKMLGERKS 224
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.133 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,226,691
Number of Sequences: 60738
Number of extensions: 797503
Number of successful extensions: 1813
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1781
Number of HSP's gapped (non-prelim): 7
length of query: 304
length of database: 30,389,216
effective HSP length: 106
effective length of query: 198
effective length of database: 23,950,988
effective search space: 4742295624
effective search space used: 4742295624
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)