ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI1332 good L KOG2267 Replication, recombination and repair Eukaryotic-type DNA primase, large subunit
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI1332 469283 470761 493
(493 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKL045w [L] KOG2267 Eukaryotic-type DNA primase large subunit 617 e-176
SPBC17D11.06 [L] KOG2267 Eukaryotic-type DNA primase large subunit 362 e-100
At1g67320_2 [L] KOG2267 Eukaryotic-type DNA primase large subunit 276 5e-74
Hs4506053 [L] KOG2267 Eukaryotic-type DNA primase large subunit 274 2e-73
CE24028 [L] KOG2267 Eukaryotic-type DNA primase large subunit 194 2e-49
ECU05g1210 [L] KOG2267 Eukaryotic-type DNA primase large subunit 93 1e-18
>YKL045w [L] KOG2267 Eukaryotic-type DNA primase large subunit
Length = 528
Score = 617 bits (1590), Expect = e-176
Identities = 306/509 (60%), Positives = 384/509 (75%), Gaps = 25/509 (4%)
Query: 1 MFRQTKKRVANRRNFVGDDQ--------------NRIDVTGYGNQDDGMQYDILYPTKLT 46
MFRQ+K+R+A+R+NF D N+I ++ ++++ Y LY +KL+
Sbjct: 1 MFRQSKRRIASRKNFSSYDDIVKSELDVGNTNAANQIILSSSSSEEEKKLYARLYESKLS 60
Query: 47 WYRLPPKGEITLEQFETWAIDRLKVLLELESLWQRNKSMKEIETTIKPVLSSKLNLGNDN 106
+Y LPP+GEITLEQFE WAIDRLK+LLE+ES RNKS+KEIET IKP L ++
Sbjct: 61 FYDLPPQGEITLEQFEIWAIDRLKILLEIESCLSRNKSIKEIETIIKPQFQKLLPFNTES 120
Query: 107 -ESRKKDYYSHFILRLCFCRSKELRDKFVRAETLLFKIRFQMLTTQDQLNFISSLDLPYV 165
E RKKDYYSHFILRLCFCRSKELR+KFVRAET LFKIRF MLT+ DQ F+ SLDLP +
Sbjct: 121 LEDRKKDYYSHFILRLCFCRSKELREKFVRAETFLFKIRFNMLTSTDQTKFVQSLDLPLL 180
Query: 166 NSIDEEEKQSLSTELYQTVSHLLTFQLNLTEETQRRQFFNREKYYKLPMENVMDLVGSRQ 225
I EEK LS +LYQTVS L FQLNL EE QR+Q+F +EK+ KLP ENV++LVG+R
Sbjct: 181 QFISNEEKAELSHQLYQTVSASLQFQLNLNEEHQRKQYFQQEKFIKLPFENVIELVGNRL 240
Query: 226 IFIKKGWAYVPQFQQLNLISNEFSDCLQTELMGTYQMIPKLNEDDRILPILHHLSSGYVV 285
+F+K G+AY+PQFQQLNL+SNEF+ L EL+ TYQ +P+LNEDDR+LPIL+HLSSGY +
Sbjct: 241 VFLKDGYAYLPQFQQLNLLSNEFASKLNQELIKTYQYLPRLNEDDRLLPILNHLSSGYTI 300
Query: 286 TE--SQELN--GAGVNDEFTHRSVKTKKVMEHFPLCAKQLMEQLQTNHHLRYQGRQQLSF 341
+ Q+ N V+DE +SV ++++ ++PLC K LME L+ NHHLRY GRQQLS
Sbjct: 301 ADFNQQKANQFSENVDDEINAQSVWSEEISSNYPLCIKNLMEGLKKNHHLRYYGRQQLSL 360
Query: 342 FLKGIGLSVDEALQFWTESFTSGGKMTVDQFNKEYKYNFRHNYGLEGNRINYKPWDCRTI 401
FLKGIGLS DEAL+FW+E+FT G MT+++FNKEY+Y+FRHNYGLEGNRINYKPWDC TI
Sbjct: 361 FLKGIGLSADEALKFWSEAFTRNGNMTMEKFNKEYRYSFRHNYGLEGNRINYKPWDCHTI 420
Query: 402 LSKPRPSRGEYHGCPYRDYHPDKLVATLKEMSLTQQQVNSVVESCQQQDYTIACTKVFEA 461
LSKPRP RG+YHGCP+RD+ ++L A L+ M LTQ Q+ SV++SCQ+ +YTIACTKVFE
Sbjct: 421 LSKPRPGRGDYHGCPFRDWSHERLSAELRSMKLTQAQIISVLDSCQKGEYTIACTKVFEM 480
Query: 462 VHKTTTADLQ------IVHPNQYFERSRQ 484
H + +ADL+ I HPN YFERSRQ
Sbjct: 481 THNSASADLEIGEQTHIAHPNLYFERSRQ 509
>SPBC17D11.06 [L] KOG2267 Eukaryotic-type DNA primase large subunit
Length = 459
Score = 362 bits (928), Expect = e-100
Identities = 211/498 (42%), Positives = 290/498 (57%), Gaps = 52/498 (10%)
Query: 1 MFRQTKKRVANRRNFVGDDQNRIDVTGYGNQDDGMQYDILYPTKLTWYRLPPKGEITLEQ 60
MFR TK RV + N + YPT+L +Y+ PP EI++E+
Sbjct: 1 MFRTTKSRVVEKNQ---------------NFSSASFESLSYPTRLNFYKKPPVSEISIEE 45
Query: 61 FETWAIDRLKVLLELESLWQRNKSMKEIETTIKPVLSSKLNLGND----------NESRK 110
FETWAIDRL VL E+ES RNK+ E+ IK +L +L + ++ +E R+
Sbjct: 46 FETWAIDRLVVLGEIESAMLRNKTKNELNGIIKKILDKRLPMSSNMARTVKGNSLDEERR 105
Query: 111 KDYYSHFILRLCFCRSKELRDKFVRAETLLFKIRFQMLTTQDQLNFISSLDLPYVNSIDE 170
KD+YSHFILRL F RS ELR +F+RAE+ LF+ RF T+++ FI SLD + S+ +
Sbjct: 106 KDHYSHFILRLAFSRSDELRIRFLRAESTLFRFRFVNEETRERQAFIDSLDFQW-ESVSQ 164
Query: 171 EEKQSLSTELYQTVSHLLTFQLNLTEETQRRQFFNREKYYKLPMENVMDLVGSRQIFIKK 230
EEK F L +QR E ++K+P V DLV R +F+ K
Sbjct: 165 EEKD--------------VFYEKLQASSQRN--IENESFFKVPFFKVPDLVERRAVFVHK 208
Query: 231 GWAYVPQFQQLNLISNEFSDCLQTELMGTYQMIPKLNEDDRILPILHHLSSGYVVTESQE 290
G+AYVP +Q++L+ EF + L+ L T + +P+L+EDDR+LPIL+HLS G+V ES
Sbjct: 209 GYAYVPFSEQVSLVVEEFEENLKKALESTAKSLPRLDEDDRLLPILNHLSKGFVAPESSI 268
Query: 291 LNGAGVNDEFTHRSVKTKKVMEHFPLCAKQLMEQLQTNHHLRYQGRQQLSFFLKGIGLSV 350
+ A + T V + + HFPLCAK L E L+ + HLR+ GR Q FLK IGLSV
Sbjct: 269 V--APKSGAITAGQVDS--FVSHFPLCAKHLHEVLKRDKHLRHFGRLQYGLFLKDIGLSV 324
Query: 351 DEALQFWTESFTSGGKMTVDQFNKEYKYNFRHNYGLEGNRINYKPWDCRTILSKPRPSRG 410
DEAL FW +SFT+ +T D+FNKEY+YN RH YGLEGNR NYK ++C+ IL+ P+ G
Sbjct: 325 DEALVFWRKSFTN---VTEDKFNKEYRYNIRHTYGLEGNRKNYKGYNCQQILTGPQLGPG 381
Query: 411 EYHGCPYRDYHPDKLVATLKEMSLTQQQV--NSVVESCQQQDYTIACTKVFEAVHKTT-T 467
+ HGCPYR Y + LV+ L M + VVE+ + + Y IACT+VFE H +
Sbjct: 382 DAHGCPYRTYSVNNLVSALASMGIAPDSAGCREVVEAVKARHYHIACTRVFELTHPNVGS 441
Query: 468 ADLQIVHPNQYFERSRQA 485
+ I HP QYF+ S ++
Sbjct: 442 LEESIHHPLQYFQLSLES 459
>At1g67320_2 [L] KOG2267 Eukaryotic-type DNA primase large subunit
Length = 464
Score = 276 bits (706), Expect = 5e-74
Identities = 163/465 (35%), Positives = 256/465 (55%), Gaps = 22/465 (4%)
Query: 38 DILYPTKLTWYRLPPKGEITLEQFETWAIDRLKVLLELESLWQRNKSMKEIETTIKPVLS 97
D++ L Y P E+ LE+FE +AIDRL+VL + R ++ E++ ++ +
Sbjct: 5 DVVSTPTLPLYLTAPHMEVRLEEFELFAIDRLRVLKGVSDGLARARNPNEMDDLVETLWK 64
Query: 98 SKLNLGNDNESRKKDYYSHFILRLCFCRSKELRDKFVRAETLLFKIRFQMLTTQDQLNFI 157
+ L N +E KD SHF+LRL +CRS EL+ F+ ET LF+ RF++ ++Q +
Sbjct: 65 EHMRLSNVSEMINKDIISHFVLRLVYCRSDELKKWFLSMETALFRHRFRLKKIEEQRAIV 124
Query: 158 SSLDLPYVNSIDEE-EKQSLSTELYQTVSHLLTFQL----NLTEETQRRQFFNREKYY-- 210
LPY I E E + L + Q++ L ++ + + + ++
Sbjct: 125 GEFGLPYKAVIGAELEVKKLIFDHLQSLKERLGLVARSHGQISSDATNSFYLIKFHFHLK 184
Query: 211 KLPMENVMDLVGSRQIFIKKGWAYVPQFQQLNLISNEFSDCLQTELMGT---YQMIPKLN 267
K+P E V DLV SR++ ++KG+A+V Q ++L+ +F L L+ T + +
Sbjct: 185 KVPFEEVPDLVASRRVLLQKGFAFVAGTQLVSLVVTQFRSHLSKALILTNRKWTTTIRER 244
Query: 268 EDDRILPILHHLSSGYVVTESQELNGAGVNDEFTHRSVK--TKKVMEHFPLCAKQLMEQL 325
E DR+ PI+ LS+ Y+ + + N E+ S+K + FPLC + L E+L
Sbjct: 245 EKDRLTPIVEALSTSYLGPDYSQSN------EYADISLKDIDQVSKSSFPLCMRHLFEKL 298
Query: 326 QTNHHLRYQGRQQLSFFLKGIGLSVDEALQFWTESFTSGGKMTVDQFNKEYKYNFRHNYG 385
+ +HHL++ GR QL FLKG+GL +D+AL FW E FT K+ ++F+KEY Y RHNYG
Sbjct: 299 REDHHLKHGGRMQLGLFLKGVGLKLDDALAFWREEFTK--KVGSERFDKEYAYAIRHNYG 356
Query: 386 LEGNRINYKPWDCRTILSKPRPSRGEYHGCPYRDYHPDKLVATLKEMSLTQQQVNSVVES 445
EG R +Y P+ C I++ P G++HGCPYR + D L A L M L+ + + V++
Sbjct: 357 KEGKRTDYTPYACSKIITS-APGAGDHHGCPYRHFSEDNLKAALSRMGLSSRGMEDVMDK 415
Query: 446 CQQQDYTIACTKVFEAVHKTTTADLQIVHPNQYFERSRQAAAAVT 490
+ + Y +ACT FEAV+ T+ D I HPNQYFE S++ + T
Sbjct: 416 VRNRHYQLACTLTFEAVY-GTSCDTGINHPNQYFEESQKILKSKT 459
>Hs4506053 [L] KOG2267 Eukaryotic-type DNA primase large subunit
Length = 509
Score = 274 bits (701), Expect = 2e-73
Identities = 155/457 (33%), Positives = 246/457 (52%), Gaps = 24/457 (5%)
Query: 34 GMQYDILYPTKLTWYRLPPKGEITLEQFETWAIDRLKVLLELESLWQR-----NKSMKEI 88
G Q + YP L +Y PP I+L +FE AIDR+K+L +E+L + ++
Sbjct: 15 GDQRNASYPHCLQFYLQPPSENISLTEFENLAIDRVKLLKSVENLGVSYVKGTEQYQSKL 74
Query: 89 ETTIKPV-LSSKLNLGNDNESRKKDYYSHFILRLCFCRSKELRDKFVRAETLLFKIRFQM 147
E+ ++ + S + L ++ E R++D+ SHFILRL +C+S+ELR F++ E L + RF +
Sbjct: 75 ESELRKLKFSYREKLEDEYEPRRRDHISHFILRLAYCQSEELRRWFIQQEMDLLRFRFSI 134
Query: 148 LTTQDQLNFISSLDLPYVNSIDEEEKQSLSTELYQTVSHLLTFQLNLTEETQRRQFFNRE 207
L +F+ L + +I +EEK E+ + L +L E
Sbjct: 135 LPKDKIQDFLKDSQLQF-EAISDEEKTLREQEIVASSPSLSGLKLGF------------E 181
Query: 208 KYYKLPMENVMDLVGSRQIFIKKGWAYVPQFQQLNLISNEFSDCLQTELMGTYQMIPKLN 267
YK+P + +DL R+++++ G+AYVP + +I NEF L L T + +P +
Sbjct: 182 SIYKIPFADALDLFRGRKVYLEDGFAYVPLKDIVAIILNEFRAKLSKALALTARSLPAVQ 241
Query: 268 EDDRILPILHHLSSGYVVTESQELNGAGVNDEFTHRSVKTKKVMEHFPLCAKQLMEQLQT 327
D+R+ P+L+HLS Y + G + TK FP C +QL + L+
Sbjct: 242 SDERLQPLLNHLSHSYTGQDYSTQGNVGKISLDQIDLLSTKS----FPPCMRQLHKALRE 297
Query: 328 NHHLRYQGRQQLSFFLKGIGLSVDEALQFWTESFTSGGKMTVDQFNKEYKYNFRHNYGLE 387
NHHLR+ GR Q FLKGIGL++++ALQFW + F G KM D+F+K Y YN RH++G E
Sbjct: 298 NHHLRHGGRMQYGLFLKGIGLTLEQALQFWKQEFIKG-KMDPDKFDKGYSYNIRHSFGKE 356
Query: 388 GNRINYKPWDCRTILSKPRPSRGEYHGCPYRDYHPDKLVATLKEMSLTQQQVNSVVESCQ 447
G R +Y P+ C I+ PS+G+YHGCP+R P+ L L+ ++ ++ +++ +
Sbjct: 357 GKRTDYTPFSCLKIILSNPPSQGDYHGCPFRHSDPELLKQKLQSYKISPGGISQILDLVK 416
Query: 448 QQDYTIACTKVFEAVHKTTTADLQIVHPNQYFERSRQ 484
Y +AC K FE +H + HPNQ+F S++
Sbjct: 417 GTHYQVACQKYFEMIHNVDDCGFSLNHPNQFFCESQR 453
>CE24028 [L] KOG2267 Eukaryotic-type DNA primase large subunit
Length = 503
Score = 194 bits (494), Expect = 2e-49
Identities = 136/463 (29%), Positives = 225/463 (48%), Gaps = 37/463 (7%)
Query: 42 PTKLTWYRLPPKGEITLEQFETWAIDRLKVLLELESLWQ---------RNKSMKEIETTI 92
P L Y+ PP +I+L +F+ A++RLK+L E+L ++K K + T
Sbjct: 38 PEYLQLYQTPPGDDISLTEFDDIAMERLKLLKSFENLKDSVSLYNDEFKDKFRKLCDGTK 97
Query: 93 KPVLSSKLNLGNDN--ESRKKDYYSHFILRLCFCRSKELRDKFVRAETLLFKIRFQMLTT 150
V + + E ++D HFILRL FCR+ E + + E + R +
Sbjct: 98 LIVFPDQKPPHQEEVYELWRRDNIGHFILRLAFCRTPENQKWLTQIEGDFLRFRLRQERE 157
Query: 151 QDQLNFISSLDLPYVNSIDEEEKQSLSTELYQTVSHLLTFQLNLTEETQRRQFFNREKYY 210
Q + +++++ + + EKQ++ +L Q NL E + +Y
Sbjct: 158 QVLDSALANVNFT-IEKLGYTEKQAMIEDLEAAC------QFNLFEAPNKT-------FY 203
Query: 211 KLPMENVMDLVGSRQIFIKKGWAYVPQFQQLNLISNEFSDCLQTELMGTYQMIPKLNEDD 270
K+ + ++L+ + +KKG+AY P + ++ + + + + +++ + L E+
Sbjct: 204 KVDFIDAIELIRRGSVHLKKGFAYFPFDDLVTILVTKMKNNMMAAMARSFKHLAILEEEG 263
Query: 271 RILPILHHLSSGYVVTESQELNGAGVNDEF-THRSVKTKKVMEHFPLCAKQLMEQLQTNH 329
R+LP L LS+ ++ NG + R + K F C KQ+ L+ NH
Sbjct: 264 RLLPRLSLLSNN--AYSGKDYNGEQPDGSIIVTRHMIDKLSRRSFAPCMKQMHNHLRVNH 321
Query: 330 HLRYQGRQQLSFFLKGIGLSVDEALQFWTESFTSGGKMTVDQFNKEYKYNFRHNYGLEGN 389
HL+Y R+Q FLKGIGLS+DEA+ + FT K+T D+F+KEY YN R+ YG EG
Sbjct: 322 HLKYGARRQYGLFLKGIGLSLDEAIAMMRDEFTK--KITSDKFDKEYGYNIRYMYGKEGR 379
Query: 390 RINYKPWDCRTILSKPRPSRGEYHGCPYRDYHPDKLVATLK---EMSLTQQQVNSVVESC 446
R+ C TI+ + PS + HGCP+R H +K V K + L ++Q+ + E
Sbjct: 380 RVAQTAMSCATIILRNPPSAVDCHGCPFR--HSEKQVLKQKLGSDAILKKEQIERIAELA 437
Query: 447 QQQDYTIACTKVFEAVHKTTTADL--QIVHPNQYFERSRQAAA 487
+ Y ACT+ FE +H L I HPNQ++E S++ A
Sbjct: 438 ELNQYDKACTRYFEFMHNLEEGGLGQLITHPNQFYEESQKIVA 480
>ECU05g1210 [L] KOG2267 Eukaryotic-type DNA primase large subunit
Length = 399
Score = 92.8 bits (229), Expect = 1e-18
Identities = 62/201 (30%), Positives = 98/201 (47%), Gaps = 20/201 (9%)
Query: 217 VMDLVGSRQIFIKKGWAYVPQFQQLNLISNEFSDCLQTELMGTYQMIPKLNEDDRILPIL 276
V+DL+G R + + G+ + +L+ +EF L + ++ NE D + +L
Sbjct: 152 VIDLIGKRAVVPEGGYLRLNDEGIGSLLVSEFRRYLSNRMDELVEI--SSNEPDERMRVL 209
Query: 277 HHLSSGYVVTESQELNGAGVNDEFTHRSVKTKKVMEHFPLCAKQLMEQLQTNHHLRYQGR 336
S+ + + G + E + R +FP C + +ME+L+ N HL+Y R
Sbjct: 210 ---STSLLANPTGYERGTNFSLESSER---------YFPPCMQDIMERLRKNKHLKYNDR 257
Query: 337 QQLSFFLKGIGLSVDEALQFWTESFTSGGKMTVDQFNKEYKYNFRHNYGLEGNRINYKPW 396
Q L FLK G+SV++ + F+ SF K + F+KEY Y+ RHNYGLEG R NY +
Sbjct: 258 QTLCLFLKDCGMSVEDGIAFFRGSF----KAPREVFDKEYLYSIRHNYGLEGKRANYSCF 313
Query: 397 DCRTILSKPRPSRGEYHGCPY 417
C I + R CP+
Sbjct: 314 SCSKIANMTNEERRS--SCPF 332
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.135 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,400,944
Number of Sequences: 60738
Number of extensions: 1278509
Number of successful extensions: 3206
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3175
Number of HSP's gapped (non-prelim): 6
length of query: 493
length of database: 30,389,216
effective HSP length: 110
effective length of query: 383
effective length of database: 23,708,036
effective search space: 9080177788
effective search space used: 9080177788
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)