ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI1400 good H KOG1536 Coenzyme transport and metabolism Biotin holocarboxylase synthetase/biotin-protein ligase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI1400 494045 496123 693
(693 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDL141w [H] KOG1536 Biotin holocarboxylase synthetase/biotin-pro... 736 0.0
SPBC30D10.07c [H] KOG1536 Biotin holocarboxylase synthetase/biot... 371 e-102
Hs19923102 [H] KOG1536 Biotin holocarboxylase synthetase/biotin-... 164 4e-40
CE27126 [H] KOG1536 Biotin holocarboxylase synthetase/biotin-pro... 122 1e-27
At2g25710 [H] KOG1536 Biotin holocarboxylase synthetase/biotin-p... 94 5e-19
At1g37150 [H] KOG1536 Biotin holocarboxylase synthetase/biotin-p... 76 2e-13
>YDL141w [H] KOG1536 Biotin holocarboxylase synthetase/biotin-protein ligase
Length = 690
Score = 736 bits (1900), Expect = 0.0
Identities = 357/694 (51%), Positives = 490/694 (70%), Gaps = 9/694 (1%)
Query: 1 MNVLVYNGAGTSPESVKNTIETLRLLLEPYYAVSPVTARVLETEPWTTKTSVVVFPGGAD 60
MNVLVYNG GT+P SVK+ +E+LR LEPYYAVS V +VL+TEPW +KTS VVFPGGAD
Sbjct: 1 MNVLVYNGPGTTPGSVKHAVESLRDFLEPYYAVSTVNVKVLQTEPWMSKTSAVVFPGGAD 60
Query: 61 LPYVRDCKKVIPKIRDFVSKDGGLYIGICAGGYFGSGRCEFAKGDPCYEVTGPRDLKLFP 120
LPYV+ C+ +I +++ FVSK GG++IG CAGGYFG+ R EFA+GDP EV+G RDL+ FP
Sbjct: 61 LPYVQACQPIISRLKHFVSKQGGVFIGFCAGGYFGTSRVEFAQGDPTMEVSGSRDLRFFP 120
Query: 121 GIARGPAFSGFKYNKEDGAKTVKLKVNGIENLQEAYSYYNGGALFVDAEKYPNVEVLAEY 180
G +RGPA++GF+YN E GA+ VKL + + + +Y+NGGA+FVDA+K+ NVE+LA Y
Sbjct: 121 GTSRGPAYNGFQYNSEAGARAVKLN---LPDGSQFSTYFNGGAVFVDADKFDNVEILATY 177
Query: 181 EDSVDVPFSDDPNXXXXXXXXXXXXIVLSSFGKGKALFIGPHPEISAKNLKPIGEEWYDN 240
+ DVP SD +VL + G+GK L GPHPE + + ++ ++ +
Sbjct: 178 AEHPDVPSSDSGKGQSENPAA----VVLCTVGRGKVLLTGPHPEFNVRFMRKSTDKHFLE 233
Query: 241 KVIKLLEEHDTSRFQLMKEVLRKAGLKCNSTESNPGNPDLTPIFVATKDNMAD-LREFEE 299
V++ L+ + R + M+ VL K GL CN+ + P+LTP+F+A+ N + L+E E
Sbjct: 234 TVVENLKAQEIMRLKFMRTVLTKTGLNCNNDFNYVRAPNLTPLFMASAPNKRNYLQEMEN 293
Query: 300 NLRSNSAKTDDSGDAYHLTGENDSFDIYEGFSHA-EEASLKLQDVHPVDAVKQIIFPANE 358
NL + ++ L E DSF Y G+ + + AS L P + K +IFP +
Sbjct: 294 NLAHHGMHANNVELCSELNAETDSFQFYRGYRASYDAASSSLLHKEPDEVPKTVIFPGVD 353
Query: 359 ETVPGKPLISHFDMQKFFAHLNPSSKLGSILLYGDVVTSTSMILDQNKTLLAALPENRAI 418
E +P +FDM+++F +LN + +GS+LLYG+VVTSTS IL+ NK+LL+++PE+ +
Sbjct: 354 EDIPPFQYTPNFDMKEYFKYLNVQNTIGSLLLYGEVVTSTSTILNNNKSLLSSIPESTLL 413
Query: 419 HVGTIQLLGRGRGGNTWVNPRGVLASTTAINLPAVSPSSGERTPIVFVQYLAMLAYCKAI 478
HVGTIQ+ GRGRGGNTW+NP+GV AST + +P SP + +VFVQYL+MLAYCKAI
Sbjct: 414 HVGTIQVSGRGRGGNTWINPKGVCASTAVVTMPLQSPVTNRNISVVFVQYLSMLAYCKAI 473
Query: 479 RTFAPGYEDLPVKIKWPNDLYAMKPQYYYNNNMKLLGKEFPNGLIPLDDVDPAFVKVSGL 538
++APG+ D+PV+IKWPNDLYA+ P YY N+KL+ F + +PL D++PA++K+SGL
Sbjct: 474 LSYAPGFSDIPVRIKWPNDLYALSPTYYKRKNLKLVNTGFEHTKLPLGDIEPAYLKISGL 533
Query: 539 LVTTNFVSGKYSLLLGCGVNVTNEAPTTSLATWVKILNNERDALGMPHLPPIDHEILLAK 598
LV T+F++ KY LLLGCG+N+T++ PTTSL TW+ ILN ER L + LP I E L A
Sbjct: 534 LVNTHFINNKYCLLLGCGINLTSDGPTTSLQTWIDILNEERQQLHLDLLPAIKAEKLQAL 593
Query: 599 YLNELDTLLKKFLMYGPSPILPEYYKYWMHSDQIVQLLDHNSVRAKLVGITEDYGLLIAK 658
Y+N L+ +LK+F+ YG + ILP YY+ W+HS+QIV L DH + +A + GITEDYGLLIAK
Sbjct: 594 YMNNLEVILKQFINYGAAEILPSYYELWLHSNQIVTLPDHGNTQAMITGITEDYGLLIAK 653
Query: 659 ELIAGSNTHFTGNVYHLQPDGNTFDIFKGLISKK 692
EL++GS+T FTGNVY+LQPDGNTFDIFK LI+KK
Sbjct: 654 ELVSGSSTQFTGNVYNLQPDGNTFDIFKSLIAKK 687
>SPBC30D10.07c [H] KOG1536 Biotin holocarboxylase synthetase/biotin-protein ligase
Length = 631
Score = 371 bits (952), Expect = e-102
Identities = 248/694 (35%), Positives = 354/694 (50%), Gaps = 66/694 (9%)
Query: 1 MNVLVYNGAGTSPESVKNTIETLRLLLEPYYAVSPVTARVLETEPWTTKTSVVVFPGGAD 60
MNVL+YNG G S + T ++L + P YA+ V A LE EPW T+++V PGG D
Sbjct: 1 MNVLIYNGNGASKICLLRTFQSLLPFVVPLYAMRFVDASTLEKEPWPASTALLVMPGGRD 60
Query: 61 LPYVRDCKKVI-PKIRDFVSKDGGLYIGICAGGYFGSGRCEFAKGDPCYEVTGPRDLKLF 119
+ Y + I KI DFV K GG Y+G+CAGGYFGS +F D V G R L+ F
Sbjct: 61 MGYCSSFNETIYRKITDFV-KRGGAYLGLCAGGYFGSAVVDFRMPDSDLNVVGKRKLQFF 119
Query: 120 PGIARGPAFSGFKYNKEDGAKTVKLKVNGIENLQ-EAYSYYNGGALFVDAEKYPNVEVLA 178
PG GP F GF Y+ EDGA+ + V+G+++ + Y+NGG F++ E Y NV+V+A
Sbjct: 120 PGTCAGPTFPGFTYDSEDGARRASIIVDGMQSSPVHTHIYFNGGGSFLETENYSNVKVVA 179
Query: 179 EYEDSVDVPFSDDPNXXXXXXXXXXXXIVLSSFGKGKALFIGPHPEISAKNLKPIGEEWY 238
Y+++ I+ GKG + G HPE SA+ PI
Sbjct: 180 RYQET---------------DFEKSAAIIYVKVGKGNVVLTGIHPEFSAEG-SPI----- 218
Query: 239 DNKVIKLLEEHDTSRFQLMKEVLRKAGLKCNSTESNPGNPDLTPIFVATKDNMADLREFE 298
L + + +R +L+ +L+ GLK S G P LT ++ N + + F
Sbjct: 219 ------LDKRDEKTRLELLSYILKLLGLKVPKDTSKCGQPTLTDQYLF--PNNVETKRFI 270
Query: 299 ENLRSNSAKTDDSGDAYHLTGENDSFDIYEGFSHAEEASLKLQDVHPVDAVKQIIFPANE 358
E +N K D Y + S +I E + S L D + VK I+ +E
Sbjct: 271 EKALTNKVKNQDEDTLYTFQFSDISSEIPEHQLANLDISADLSD-SDNEIVK--IWYGDE 327
Query: 359 ETVPGKPLISHFDMQKFFAHLNPSSKLGSILLYGDVVTSTSMILDQNKTLLAALPENRAI 418
E + K S FD++ +A L + G ++ V+ ST +LD+N L + +
Sbjct: 328 EKICKKAKPS-FDLE-LYAKLINGCRFGLPIIVAPVIRSTQTLLDKNYRFLDSTNTGFTV 385
Query: 419 HVGTIQLLGRGRGGNTWVNPRGVLASTTAINLPAVSPSSGERTPIVFVQYLAMLAYCKAI 478
+G Q GRGRG N WV+P G LA + IN+ A + S+ TPI QYL LA + I
Sbjct: 386 -LGNYQTAGRGRGQNMWVSPYGTLAFSFIINVDAKNFST---TPIALFQYLMALAVVRGI 441
Query: 479 RTFAPGYEDLPVKIKWPNDLYAMKPQYYYNNNMKLLGKEFPNGLIPLDDVDPAFVKVSGL 538
R +APGYE++P IKWPND+Y + + GK+ ++K+SG+
Sbjct: 442 REYAPGYENIPAFIKWPNDVYVRVDK----GGINFQGKQ--------------YMKLSGI 483
Query: 539 LVTTNFVSGKYSLLLGCGVNVTNEAPTTSLATWVKILNNERDALGMPHLPPIDHEILLAK 598
+VT+N+ L++GCG+NV+N PT SL T V N D P L E LLA
Sbjct: 484 IVTSNYRKNVLHLVVGCGINVSNLGPTVSLNTLVDEWNKNSD---NPRLEKFSFEKLLAS 540
Query: 599 YLNELDTLLKKFLMYGPSPILPEYYKYWMHSDQIVQLLDHNSVRAKLVGITEDYGLLIAK 658
LN+ D + L G S ILPEYY+YW+HS+Q V L + +A + GIT D+G L+A+
Sbjct: 541 VLNQFDRYHRLLLEEGFSLILPEYYQYWLHSNQTVNLA--SGGKAIIQGITSDFGFLLAQ 598
Query: 659 ELIAGSNTHFTGNVYHLQPDGNTFDIFKGLISKK 692
+ N T V HLQPDGN+FD+ + LI++K
Sbjct: 599 --LLNENNEPTTKVVHLQPDGNSFDLMRNLITRK 630
>Hs19923102 [H] KOG1536 Biotin holocarboxylase synthetase/biotin-protein ligase
Length = 726
Score = 164 bits (415), Expect = 4e-40
Identities = 128/451 (28%), Positives = 217/451 (47%), Gaps = 81/451 (17%)
Query: 245 LLEEHDTSRFQLMKEVLRKAGLKCNSTESNPGNPDLTPIFVATKDNMADLREFEENLRSN 304
LL+ + R+++++E+L GL C+ + P LTP+++ ++ E + L
Sbjct: 352 LLKSSNFRRYEVLREILTTLGLSCDMKQV----PALTPLYL-----LSAAEEIRDPLMQW 402
Query: 305 SAKTDDSGDAYHLTGENDSFDIYEGFSHAEEASLKLQDVHPVDAVKQIIFPANEETVPGK 364
K DS EG + + SL+ + V V+ I P+ V
Sbjct: 403 LGKHVDS----------------EGEIKSGQLSLRFVSSY-VSEVE--ITPSCIPVVTNM 443
Query: 365 PLIS--HFDMQKFFAHLNPSSKLGSILLYGDVVTSTSMILDQNKTLLAALPENRA-IHVG 421
S HF+++ + +L + +LG ++L+ +V +T +LD L+ P+ I +
Sbjct: 444 EAFSSEHFNLEIYRQNLQ-TKQLGKVILFAEVTPTTMRLLDG---LMFQTPQEMGLIVIA 499
Query: 422 TIQLLGRGRGGNTWVNPRGVLASTTAINLPAVSPSSGERTPIVFVQYLAMLAYCKAIRTF 481
Q G+GRGGN W++P G ST I++P S G+R P FVQ+L +A +A+R+
Sbjct: 500 ARQTEGKGRGGNVWLSPVGCALSTLLISIPLRS-QLGQRIP--FVQHLMSVAVVEAVRSI 556
Query: 482 APGYEDLPVKIKWPNDLYAMKPQYYYNNNMKLLGKEFPNGLIPLDDVDPAFVKVSGLLVT 541
P Y+D+ +++KWPND+Y Y++ MK + G+LV
Sbjct: 557 -PKYQDINLRVKWPNDIY-------YSDLMK----------------------IGGVLVN 586
Query: 542 TNFVSGKYSLLLGCGVNVTNEAPTTSLATWVKILNNERDALGMPHLPPIDHEILLAKYLN 601
+ + + +L+GCG NVTN PT + + N + A L P+ + L+A+ +
Sbjct: 587 STLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKA----ELKPLRADYLIARVVT 642
Query: 602 ELDTLLKKFLMYGPSPILPEYYKYWMHSDQIVQLLDHNSVRAKLVGITEDYGLLIAKELI 661
L+ L+K+F GP+ +LP YY+YW+HS Q V L + +VG+ + L + +E
Sbjct: 643 VLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQE-- 700
Query: 662 AGSNTHFTGNVYHLQPDGNTFDIFKGLISKK 692
G V + PDGN+FD+ + LI K
Sbjct: 701 -------GGEVVTVHPDGNSFDMLRNLILPK 724
>CE27126 [H] KOG1536 Biotin holocarboxylase synthetase/biotin-protein ligase
Length = 1025
Score = 122 bits (307), Expect = 1e-27
Identities = 92/324 (28%), Positives = 150/324 (45%), Gaps = 55/324 (16%)
Query: 369 HFDMQKFFAHLNPSSKLGSILLYGDVVTSTSMILDQNKTLLAALPENRAIHVGTIQLLGR 428
+FD +F ++ SK+G ++L DV T+T I++ + +L + + Q+ GR
Sbjct: 756 NFDFNLYFEQIH--SKIGQVVLLVDVATTTMDIIESVNAGIPSLES--VVVIANRQISGR 811
Query: 429 GRGGNTWVNPRGVLASTTAINLPAVSPSSGERTPIVFVQYLAMLAYCKAIRTFAPGYEDL 488
GRGGN ++ PRG+ + + +S S + +Q++ +A +A R + GY +
Sbjct: 812 GRGGNEFLCPRGMAMFNFSFS---ISKKSRIAKHLPILQHIFCVALVEAARNLS-GYPEF 867
Query: 489 PVKIKWPNDLYAMKPQYYYNNNMKLLGKEFPNGLIPLDDVDPAFVKVSGLLVTTNFVSGK 548
P+ IKWPNDLY + KV G+L+ +
Sbjct: 868 PLHIKWPNDLYCERSH-----------------------------KVGGMLLQCSTRDDS 898
Query: 549 YSLLLGCGVNVTNEAPTTSLATWVKILNNERDALGMPHLPPIDHEILLAKYLNELDTLLK 608
+ + +GCG+NV+N+ PT L D L I E L+A+ +N+ +K
Sbjct: 899 FRVSIGCGMNVSNDKPTMCL----------NDMLPKEAETRITKEQLIAETINKFTYYMK 948
Query: 609 KFLMYGPSPILPEYYKYWMHSDQIVQLLDHNSVRAKLVGITEDYGLLIAKELIAGSNTHF 668
+ GP +Y++YW+HS Q V L D N R + GI +D L + + SN
Sbjct: 949 DYEDNGPETFKKKYHEYWLHSQQEVLLSDFNE-RVTIRGIDDDGYLQVRSK----SN--- 1000
Query: 669 TGNVYHLQPDGNTFDIFKGLISKK 692
++ + DGNTFD+ KGLI K
Sbjct: 1001 PDKIFSIGDDGNTFDMMKGLIRHK 1024
>At2g25710 [H] KOG1536 Biotin holocarboxylase synthetase/biotin-protein ligase
Length = 355
Score = 94.4 bits (233), Expect = 5e-19
Identities = 81/300 (27%), Positives = 136/300 (45%), Gaps = 57/300 (19%)
Query: 384 KLGSILLYGDVVTSTSMILDQNKTLLAALPENRAIHVGTIQLLGRGRGGNTWVNPRGVLA 443
+ G L++ ++ST ++ N + LP ++ V IQ GRGR N W +P+G L
Sbjct: 109 RFGRFLIWSPRLSSTHDVVSHN---FSELPVG-SVCVTDIQFKGRGRTKNVWESPKGCLM 164
Query: 444 STTAINLPAVSPSSGERTPIVFVQYLAMLAYCKAIRTFAP--GYEDLPVKIKWPNDLYAM 501
+ + + G P++ QY+ LA +A++ G + VKIKWPNDLY
Sbjct: 165 YSFTLEM-----EDGRVVPLI--QYVVSLAVTEAVKDVCDKKGLPYIDVKIKWPNDLYV- 216
Query: 502 KPQYYYNNNMKLLGKEFPNGLIPLDDVDPAFVKVSGLLVTTNFVSGKYSLLLGCGVNVTN 561
NGL KV G+L T+ + S K+++ +G G+NV N
Sbjct: 217 ------------------NGL-----------KVGGILCTSTYRSKKFNVSVGVGLNVDN 247
Query: 562 EAPTTSLATWVKILNNERDALGMPHLPPIDHEILLAKYLNELDTLLKKFLMYGPSPILPE 621
PTT L +K + E + L E +L + ++ + F+ G +
Sbjct: 248 GQPTTCLNAVLKGMAPESNLL--------KREEILGAFFHKFEKFFDLFMDQGFKSLEEL 299
Query: 622 YYKYWMHSDQIVQLLDHNSVRAKLV-GITEDYGLLIAKELIAGSNTHFTGNVYHLQPDGN 680
YY+ W+HS+Q +++ + V ++V + GL + L+A + + +Y L PDGN
Sbjct: 300 YYRTWLHSEQ--RVIVEDKVEDQVVQNVVTIQGLTSSGYLLAVGDDN---QMYELHPDGN 354
>At1g37150 [H] KOG1536 Biotin holocarboxylase synthetase/biotin-protein ligase
Length = 292
Score = 75.9 bits (185), Expect = 2e-13
Identities = 61/210 (29%), Positives = 97/210 (46%), Gaps = 48/210 (22%)
Query: 368 SHFDMQKFFAHLNPSSKLGSILLYGDVVTSTSMILDQNKTLLAALPENRAIHVGTIQLLG 427
S F++ F ++ + + G L++ ++ST ++ N + +P ++ V IQL G
Sbjct: 56 SSFNLSLFMNSIS-THRFGRFLIWSPYLSSTHDVVSHN---FSEIPVG-SVCVSDIQLKG 110
Query: 428 RGRGGNTWVNPRGVLASTTAINLPAVSPSSGERTPIVFVQYLAMLAYCKAIRTFAP---- 483
RGR N W +P+G L + + + G P++ QY+ LA +A++
Sbjct: 111 RGRTKNVWESPKGCLMYSFTLEM-----EDGRVVPLI--QYVVSLAVTEAVKDVCDKKGL 163
Query: 484 GYEDLPVKIKWPNDLYAMKPQYYYNNNMKLLGKEFPNGLIPLDDVDPAFVKVSGLLVTTN 543
Y D VKIKWPNDLY NGL K+ G+L T+
Sbjct: 164 SYND--VKIKWPNDLYL-------------------NGL-----------KIGGILCTST 191
Query: 544 FVSGKYSLLLGCGVNVTNEAPTTSLATWVK 573
+ S K+ + +G G+NV NE PTT L +K
Sbjct: 192 YRSRKFLVSVGVGLNVDNEQPTTCLNAVLK 221
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.138 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 44,776,556
Number of Sequences: 60738
Number of extensions: 2084378
Number of successful extensions: 4512
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 4478
Number of HSP's gapped (non-prelim): 8
length of query: 693
length of database: 30,389,216
effective HSP length: 113
effective length of query: 580
effective length of database: 23,525,822
effective search space: 13644976760
effective search space used: 13644976760
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)