ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactVI1504   suspect: LH   S     KOG4539  Function unknown                                               Uncharacterized conserved protein

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactVI1504 534867 533980 -296 
         (296 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YDL183c [S] KOG4539 Uncharacterized conserved protein                 221   1e-57
SPAC23H3.12c [S] KOG4539 Uncharacterized conserved protein             49   9e-06

>YDL183c [S] KOG4539 Uncharacterized conserved protein
          Length = 320

 Score =  221 bits (562), Expect = 1e-57
 Identities = 126/297 (42%), Positives = 180/297 (60%), Gaps = 20/297 (6%)

Query: 9   AYSKAYNKSSTIQQYVNDPVKLLVIPITNKQSFIYFKYSNELLNKESALIRYETKLSQKA 68
           AYS    K+S +Q+Y++DPVK+ VIPIT+K+SFIY+K+++ L N +S +++ E  + +K+
Sbjct: 24  AYSTKLEKAS-LQKYLHDPVKVTVIPITDKESFIYYKHTDNLFNSQSRILKAEKWIVEKS 82

Query: 69  ANLWYSVQASQKSYNKKIVSTVTKLLDQIPWTENSLRTIPSESYILKQIENGTKD----- 123
           A LW  ++ S KSYNKKIVS V  LL+  PW+ENSL TIPSESYILK+I+ G KD     
Sbjct: 83  AKLWRKLKKSPKSYNKKIVSMVQSLLNSTPWSENSLLTIPSESYILKRIK-GEKDKTQEI 141

Query: 124 NVTLNEY-VKAKNDDSVISLKPKPIHVYFPRSVVDESTIIAQLKQLSKKGQQYHKKHALL 182
            +TL +Y VKA+  D+      +P+HVY+P  +      + Q+K+L ++G  YHKK  L 
Sbjct: 142 RLTLKDYTVKAEQVDT------QPLHVYYPPGISSPDECLRQMKKLYQEGLIYHKKWTLY 195

Query: 183 CAXXXXXXXXXXXXXXXXXXXXXYLTYRLYCNLKAYFGAKHLESLITSEEQKFEFINLEQ 242
           C                      YL+YR Y N+KAY GAKHL+SL+ S +Q  EF  L  
Sbjct: 196 CLLGLPLTIPLILIPLIPNVPGFYLSYRAYVNIKAYLGAKHLKSLLESSKQNLEFRELLG 255

Query: 243 YSDILKESK------KEKHESDGEKLLLSEHDLDRVLDSLEIHEMSTDLRKAIRQES 293
           Y+++ K          +K      +LLL++  L  +LD LE+HE+ +DL K I QES
Sbjct: 256 YTEVYKRGTSSRTQGNQKESKGAPELLLNKKTLPLILDFLEVHELESDLNKVILQES 312


>SPAC23H3.12c [S] KOG4539 Uncharacterized conserved protein
          Length = 226

 Score = 48.9 bits (115), Expect = 9e-06
 Identities = 47/204 (23%), Positives = 88/204 (43%), Gaps = 31/204 (15%)

Query: 28  VKLLVIPITNKQSFIYFKYSNELLNKESALIRYETKLSQKAANLWYSVQASQKSYNKKIV 87
           ++++ +P+ N++ F++  Y +E L K+   +    K+  +    W S  AS+    +K+V
Sbjct: 1   MRIIALPLPNQRVFLHC-YPSEYLAKK---VTIHDKIINRIYKYWDSWSASKSYTKQKVV 56

Query: 88  STVTKLLDQIPWTENSLRTIPSESYILKQIENGTKDNVTLNEYVKAKNDDSVISLKPKPI 147
           S   ++L   P+ EN LR I                       VK  ND  +     + +
Sbjct: 57  SLGNRILHATPYEENFLRAIAP---------------------VKKLNDTEL----HQTL 91

Query: 148 HVYFPRSVVDESTIIAQLKQLSKKGQQYHKKHALLCAXXXXXXXXXXXXXXXXXXXXXYL 207
           ++  P + ++ STI+A+L + SK+ Q+ H  + +                        YL
Sbjct: 92  YIEHPPN-LESSTILAELNR-SKQLQKTHTNYLIGNIIGLPLTIPFILIPLIPNIPGFYL 149

Query: 208 TYRLYCNLKAYFGAKHLESLITSE 231
            YR YCN +A  G+  L  +++ E
Sbjct: 150 CYRAYCNFRAIQGSIQLARVMSIE 173


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.313    0.130    0.352 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,202,329
Number of Sequences: 60738
Number of extensions: 595083
Number of successful extensions: 1895
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1890
Number of HSP's gapped (non-prelim): 2
length of query: 296
length of database: 30,389,216
effective HSP length: 106
effective length of query: 190
effective length of database: 23,950,988
effective search space: 4550687720
effective search space used: 4550687720
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)