ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI1527 good E KOG2367 Amino acid transport and metabolism Alpha-isopropylmalate synthase/homocitrate synthase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI1527 541146 542468 441
(441 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDL131w [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate s... 765 0.0
YDL182w [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate s... 740 0.0
SPBC1105.02c [E] KOG2367 Alpha-isopropylmalate synthase/homocitr... 617 e-176
At5g23020 [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate... 154 3e-37
At5g23010 [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate... 153 4e-37
At1g74040 [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate... 139 6e-33
At1g18500 [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate... 132 1e-30
>YDL131w [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate synthase
Length = 440
Score = 765 bits (1975), Expect = 0.0
Identities = 383/440 (87%), Positives = 401/440 (91%), Gaps = 2/440 (0%)
Query: 1 MSSNQDFQPVTESASSVTKFQQNPYGPNPADYLSNVNNYQLIDSTLREGEQFANAFFDTE 60
MS N +FQ VTES ++ T NPYGPNPADYLSNV N+QLIDSTLREGEQFANAFFDTE
Sbjct: 1 MSENNEFQSVTESTTAPTT--SNPYGPNPADYLSNVKNFQLIDSTLREGEQFANAFFDTE 58
Query: 61 KKIEIAKALDDFGVDYIELTSPVASEQSRRDCEAICKLGLKAKILTHIRCHMDDARVAVE 120
KKIEIA+ALDDFGVDYIELTSPVASEQSR+DCEAICKLGLKAKILTHIRCHMDDARVAVE
Sbjct: 59 KKIEIARALDDFGVDYIELTSPVASEQSRKDCEAICKLGLKAKILTHIRCHMDDARVAVE 118
Query: 121 TXXXXXXXXIGTSKFLRQYSHGKDMNYIAKSAIEVIEFVKSKGIEIRFSSEDSFRSDLVD 180
T IGTSKFLRQYSHGKDMNYIAKSA+EVIEFVKSKGIEIRFSSEDSFRSDLVD
Sbjct: 119 TGVDGVDVVIGTSKFLRQYSHGKDMNYIAKSAVEVIEFVKSKGIEIRFSSEDSFRSDLVD 178
Query: 181 LLNIYKTVDKIGVNRVGIADTVGCANPRQVYELVRTLKSVVSCDIECHFHNDTGCAIANA 240
LLNIYKTVDKIGVNRVGIADTVGCANPRQVYEL+RTLKSVVSCDIECHFHNDTGCAIANA
Sbjct: 179 LLNIYKTVDKIGVNRVGIADTVGCANPRQVYELIRTLKSVVSCDIECHFHNDTGCAIANA 238
Query: 241 YTALEGGARLIDVAVLGIGERNGITPLGGLMARMIVAAPEYTKSKYKLHKIRDIENLIAE 300
YTALEGGARLIDV+VLGIGERNGITPLGGLMARMIVAAP+Y +SKYKLHKIRDIENL+A+
Sbjct: 239 YTALEGGARLIDVSVLGIGERNGITPLGGLMARMIVAAPDYVRSKYKLHKIRDIENLVAD 298
Query: 301 AVEVNIPFNNPITGFCAFTHKAGIHAKAILANPSTYEILDPHDFGMKRYIHFANRLTGWN 360
AVEVNIPFNNPITGFCAFTHKAGIHAKAILANPSTYEILDPHDFGMKRYIHFANRLTGWN
Sbjct: 299 AVEVNIPFNNPITGFCAFTHKAGIHAKAILANPSTYEILDPHDFGMKRYIHFANRLTGWN 358
Query: 361 AIKSRVDQLNLNLTDDQVKEVTAKIKKLGDIRPLNIDDVDSIIKDFHAEVSTPQLRAXXX 420
AIKSRVDQLNLNLTDDQ+KEVTAKIKKLGD+RPLNIDDVDSIIKDFHAE+STP L+
Sbjct: 359 AIKSRVDQLNLNLTDDQIKEVTAKIKKLGDVRPLNIDDVDSIIKDFHAELSTPLLKPVNK 418
Query: 421 XXXXXXXXXXQEPSNKKTKV 440
+KK KV
Sbjct: 419 GTDDDNIDISNGHVSKKAKV 438
>YDL182w [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate synthase
Length = 428
Score = 740 bits (1911), Expect = 0.0
Identities = 365/399 (91%), Positives = 382/399 (95%)
Query: 17 VTKFQQNPYGPNPADYLSNVNNYQLIDSTLREGEQFANAFFDTEKKIEIAKALDDFGVDY 76
+T + NPY P DYLSNVNN+QLIDSTLREGEQFANAFFDTEKKIEIA+ALDDFGVDY
Sbjct: 1 MTAAKPNPYAAKPGDYLSNVNNFQLIDSTLREGEQFANAFFDTEKKIEIARALDDFGVDY 60
Query: 77 IELTSPVASEQSRRDCEAICKLGLKAKILTHIRCHMDDARVAVETXXXXXXXXIGTSKFL 136
IELTSPVASEQSR+DCEAICKLGLKAKILTHIRCHMDDA+VAVET IGTSKFL
Sbjct: 61 IELTSPVASEQSRKDCEAICKLGLKAKILTHIRCHMDDAKVAVETGVDGVDVVIGTSKFL 120
Query: 137 RQYSHGKDMNYIAKSAIEVIEFVKSKGIEIRFSSEDSFRSDLVDLLNIYKTVDKIGVNRV 196
RQYSHGKDMNYIAKSA+EVIEFVKSKGIEIRFSSEDSFRSDLVDLLNIYKTVDKIGVNRV
Sbjct: 121 RQYSHGKDMNYIAKSAVEVIEFVKSKGIEIRFSSEDSFRSDLVDLLNIYKTVDKIGVNRV 180
Query: 197 GIADTVGCANPRQVYELVRTLKSVVSCDIECHFHNDTGCAIANAYTALEGGARLIDVAVL 256
GIADTVGCANPRQVYEL+RTLKSVVSCDIECHFHNDTGCAIANAYTALEGGARLIDV+VL
Sbjct: 181 GIADTVGCANPRQVYELIRTLKSVVSCDIECHFHNDTGCAIANAYTALEGGARLIDVSVL 240
Query: 257 GIGERNGITPLGGLMARMIVAAPEYTKSKYKLHKIRDIENLIAEAVEVNIPFNNPITGFC 316
GIGERNGITPLGGLMARMIVAAP+Y KSKYKLHKIRDIENL+A+AVEVNIPFNNPITGFC
Sbjct: 241 GIGERNGITPLGGLMARMIVAAPDYVKSKYKLHKIRDIENLVADAVEVNIPFNNPITGFC 300
Query: 317 AFTHKAGIHAKAILANPSTYEILDPHDFGMKRYIHFANRLTGWNAIKSRVDQLNLNLTDD 376
AFTHKAGIHAKAILANPSTYEILDPHDFGMKRYIHFANRLTGWNAIK+RVDQLNLNLTDD
Sbjct: 301 AFTHKAGIHAKAILANPSTYEILDPHDFGMKRYIHFANRLTGWNAIKARVDQLNLNLTDD 360
Query: 377 QVKEVTAKIKKLGDIRPLNIDDVDSIIKDFHAEVSTPQL 415
Q+KEVTAKIKKLGD+R LNIDDVDSIIK+FHAEVSTPQ+
Sbjct: 361 QIKEVTAKIKKLGDVRSLNIDDVDSIIKNFHAEVSTPQV 399
>SPBC1105.02c [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate synthase
Length = 418
Score = 617 bits (1590), Expect = e-176
Identities = 298/389 (76%), Positives = 340/389 (86%)
Query: 23 NPYGPNPADYLSNVNNYQLIDSTLREGEQFANAFFDTEKKIEIAKALDDFGVDYIELTSP 82
NPYGPNP+D+LS VNN+ +I+STLREGEQFANAFFDTEKKI+IAKALD+FGVDYIELTSP
Sbjct: 19 NPYGPNPSDFLSRVNNFSIIESTLREGEQFANAFFDTEKKIQIAKALDNFGVDYIELTSP 78
Query: 83 VASEQSRRDCEAICKLGLKAKILTHIRCHMDDARVAVETXXXXXXXXIGTSKFLRQYSHG 142
VASEQSR+DCEAICKLGLK KILTHIRCHMDDARVAVET IGTS++LR+YSHG
Sbjct: 79 VASEQSRQDCEAICKLGLKCKILTHIRCHMDDARVAVETGVDGVDVVIGTSQYLRKYSHG 138
Query: 143 KDMNYIAKSAIEVIEFVKSKGIEIRFSSEDSFRSDLVDLLNIYKTVDKIGVNRVGIADTV 202
KDM YI SA EVI FVKSKGIE+RFSSEDSFRSDLVDLL++YK VDKIGVNRVGIADTV
Sbjct: 139 KDMTYIIDSATEVINFVKSKGIEVRFSSEDSFRSDLVDLLSLYKAVDKIGVNRVGIADTV 198
Query: 203 GCANPRQVYELVRTLKSVVSCDIECHFHNDTGCAIANAYTALEGGARLIDVAVLGIGERN 262
GCA PRQVY+L+RTL+ VVSCDIECHFHNDTG AIANAY ALE GA ID ++LGIGERN
Sbjct: 199 GCATPRQVYDLIRTLRGVVSCDIECHFHNDTGMAIANAYCALEAGATHIDTSILGIGERN 258
Query: 263 GITPLGGLMARMIVAAPEYTKSKYKLHKIRDIENLIAEAVEVNIPFNNPITGFCAFTHKA 322
GITPLG L+ARM V EY KYKL+++R++ENL+A+AVEV IPFNN ITG CAFTHKA
Sbjct: 259 GITPLGALLARMYVTDREYITHKYKLNQLRELENLVADAVEVQIPFNNYITGMCAFTHKA 318
Query: 323 GIHAKAILANPSTYEILDPHDFGMKRYIHFANRLTGWNAIKSRVDQLNLNLTDDQVKEVT 382
GIHAKAILANPSTYEIL P DFGM RY+H +RLTGWNAIKSR +QLNL+LTD Q KE+T
Sbjct: 319 GIHAKAILANPSTYEILKPEDFGMSRYVHVGSRLTGWNAIKSRAEQLNLHLTDAQAKELT 378
Query: 383 AKIKKLGDIRPLNIDDVDSIIKDFHAEVS 411
+IKKL D+R L +DDVD +++++HA++S
Sbjct: 379 VRIKKLADVRTLAMDDVDRVLREYHADLS 407
>At5g23020 [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate synthase
Length = 503
Score = 154 bits (389), Expect = 3e-37
Identities = 123/441 (27%), Positives = 207/441 (46%), Gaps = 37/441 (8%)
Query: 2 SSNQDFQPVTESASSVTKFQQNPYGPNPADYLSNVNNYQLIDSTLREGEQFANAFFDTEK 61
+S D +P+ E ++ Y PN L + N +++D+TLR+GEQ A +
Sbjct: 59 TSATDLKPIME--------RRPEYIPNK---LPHKNYVRVLDTTLRDGEQSPGAALTPPQ 107
Query: 62 KIEIAKALDDFGVDYIELTSPVASEQSRRDCEAICKL---------GLKAKILTHIRCHM 112
K+EIA+ L VD +E+ PV+SE+ + I K G I RC
Sbjct: 108 KLEIARQLAKLRVDIMEVGFPVSSEEEFEAIKTIAKTVGNEVDEETGYVPVICGIARCKK 167
Query: 113 DDARVAVETXXXXXXXXI----GTSKFLRQYSHGKDMNYIAKSAIEVIEFVKSKGI-EIR 167
D E + TS+ +Y K + + A+ +++ KS G +I+
Sbjct: 168 RDIEATWEALKYAKRPRVMLFTSTSEIHMKYKLKKTKEEVIEMAVNSVKYAKSLGFKDIQ 227
Query: 168 FSSEDSFRSDLVDLLNIYKTVDKIGVNRVGIADTVGCANPRQVYELVR-TLKSVVSCD-- 224
F ED R++ + I K G VG ADTVG P++ ELV +++ D
Sbjct: 228 FGCEDGGRTEKDFICKILGESIKAGATTVGFADTVGINMPQEFGELVAYVIENTPGADDI 287
Query: 225 -IECHFHNDTGCAIANAYTALEGGARLIDVAVLGIGERNGITPLGGLMARMIVAAPEYTK 283
H HND G A AN + + GAR ++V + GIGER+G PL ++ +
Sbjct: 288 VFAIHCHNDLGVATANTISGICAGARQVEVTINGIGERSGNAPLEEVVMALKCRGESLMD 347
Query: 284 SKYKLHKIRDI---ENLIAEAVEVNIPFNNPITGFCAFTHKAGIHAKAILANPSTYEILD 340
Y R I ++ E + + + PI G F H++GIH IL N STYEIL
Sbjct: 348 GVYTKIDSRQIMATSKMVQEHTGMYVQPHKPIVGDNCFVHESGIHQDGILKNRSTYEILS 407
Query: 341 PHDFGMKRYIH---FANRLTGWNAIKSRVDQLNLNLTDDQVKEVTAKIKKL-GDIRPLNI 396
P D G+ + + +L+G +A+K R+ +L ++D++ ++ ++ ++L D + +
Sbjct: 408 PEDVGIVKSENSGIVLGKLSGRHAVKDRLKELGYEISDEKFNDIFSRYRELTKDKKRITD 467
Query: 397 DDVDSIIKDFHAEVSTPQLRA 417
D+ +++ + AE+S+ +L +
Sbjct: 468 ADLKALVVN-GAEISSEKLNS 487
>At5g23010 [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate synthase
Length = 506
Score = 153 bits (387), Expect = 4e-37
Identities = 124/411 (30%), Positives = 181/411 (43%), Gaps = 35/411 (8%)
Query: 2 SSNQDFQPVTESASSVTKFQQNPYGPNPADYLSNVNNYQLIDSTLREGEQFANAFFDTEK 61
+S+ D +PV E + Y PN L + N ++ D+TLR+GEQ +
Sbjct: 59 TSSTDLKPVVE--------RWPEYIPNK---LPDGNYVRVFDTTLRDGEQSPGGSLTPPQ 107
Query: 62 KIEIAKALDDFGVDYIELTSPVASEQSRRDCEAICKL---------GLKAKILTHIRCHM 112
K+EIA+ L VD +E+ P +SE+ + I K G I RC
Sbjct: 108 KLEIARQLAKLRVDIMEVGFPGSSEEELETIKTIAKTVGNEVDEETGYVPVICAIARCKH 167
Query: 113 DDARVAVETXXXXXXXXI----GTSKFLRQYSHGKDMNYIAKSAIEVIEFVKSKGI-EIR 167
D E I TS +Y K + + A+ I F KS G +I+
Sbjct: 168 RDIEATWEALKYAKRPRILVFTSTSDIHMKYKLKKTQEEVIEMAVSSIRFAKSLGFNDIQ 227
Query: 168 FSSEDSFRSDLVDLLNIYKTVDKIGVNRVGIADTVGCANPRQVYELVRTLKS----VVSC 223
F ED RSD L I K GV V I DTVG P + ELV LK+ +
Sbjct: 228 FGCEDGGRSDKDFLCKILGEAIKAGVTVVTIGDTVGINMPHEYGELVTYLKANTPGIDDV 287
Query: 224 DIECHFHNDTGCAIANAYTALEGGARLIDVAVLGIGERNGITPLGGLMARMIVAAPEYTK 283
+ H HND G A AN+ + GAR ++V + GIGER+G L ++ +
Sbjct: 288 VVAVHCHNDLGLATANSIAGIRAGARQVEVTINGIGERSGNASLEEVVMALKCRGAYVIN 347
Query: 284 SKYKLHKIRDI---ENLIAEAVEVNIPFNNPITGFCAFTHKAGIHAKAILANPSTYEILD 340
Y R I ++ E + + + PI G F H++GIH IL N STYEIL
Sbjct: 348 GVYTKIDTRQIMATSKMVQEYTGLYVQAHKPIVGANCFVHESGIHQDGILKNRSTYEILS 407
Query: 341 PHDFGMKRYIH---FANRLTGWNAIKSRVDQLNLNLTDDQVKEVTAKIKKL 388
P D G+ + + +L+G +A+K R+ +L L D+++ V + + L
Sbjct: 408 PEDIGIVKSQNSGLVLGKLSGRHAVKDRLKELGYELDDEKLNAVFSLFRDL 458
>At1g74040 [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate synthase
Length = 631
Score = 139 bits (351), Expect = 6e-33
Identities = 112/406 (27%), Positives = 184/406 (44%), Gaps = 27/406 (6%)
Query: 33 LSNVNNYQLIDSTLREGEQFANAFFDTEKKIEIAKALDDFGVDYIELTSPVASEQSRRDC 92
+S+ N ++ D+TLR+GEQ A +++K++IA+ L GVD IE P AS+
Sbjct: 81 ISDPNYVRIFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAASKDDFEAV 140
Query: 93 EAICKL--------GLKAKILTHIRCHMDDARVAVETXXXX----XXXXIGTSKFLRQYS 140
+ I + G I RC+ D A E I TS +Y
Sbjct: 141 KTIAETVGNTVDENGYVPVICGLSRCNKKDIETAWEAVKYAKRPRIHTFIATSDIHLKYK 200
Query: 141 HGKDMNYIAKSAIEVIEFVKSKGIE-IRFSSEDSFRSDLVDLLNIYKTVDKIGVNRVGIA 199
K + + A ++ F +S G E + FS ED+ RS+ L I V K G + I
Sbjct: 201 LKKSKEEVIEIARNMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKAGATTLNIP 260
Query: 200 DTVGCANPRQVYELVRTLKS----VVSCDIECHFHNDTGCAIANAYTALEGGARLIDVAV 255
DTVG P + +L+ +K+ + + I H ND G + AN + GAR ++V +
Sbjct: 261 DTVGITLPSEFGQLIADIKANTPGIQNVIISTHCQNDLGLSTANTLSGAHSGARQVEVTI 320
Query: 256 LGIGERNGITPLGGLMARMIVAAPEYTKSKYKLHKIRDI---ENLIAEAVEVNIPFNNPI 312
GIGER G L ++ + + R I ++ E + + I
Sbjct: 321 NGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAI 380
Query: 313 TGFCAFTHKAGIHAKAILANPSTYEILDPHDFGMKRYIH---FANRLTGWNAIKSRVDQL 369
G AF H++GIH +L + TYEI+ P + G++R +L+G +A+K R+++L
Sbjct: 381 VGANAFAHESGIHQDGMLKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGRHALKDRLNEL 440
Query: 370 NLNLTDDQVKEVTAKIKKLGDIRPLNID-DVDSIIKDFHAEVSTPQ 414
L D Q+ + + K + + + D D+ +++ D EV P+
Sbjct: 441 GYVLDDGQLSNLFWRFKAVAEQKKRVTDADLIALVSD---EVFQPE 483
>At1g18500 [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate synthase
Length = 660
Score = 132 bits (331), Expect = 1e-30
Identities = 107/377 (28%), Positives = 170/377 (44%), Gaps = 26/377 (6%)
Query: 24 PYGPNPA---DYLSNVNNYQLIDSTLREGEQFANAFFDTEKKIEIAKALDDFGVDYIELT 80
P P P + +S+ N ++ D+TLR+GEQ A +++K++IA+ L GVD IE
Sbjct: 71 PRRPRPEYIPNRISDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAG 130
Query: 81 SPVASEQSRRDCEAICKL--------GLKAKILTHIRCHMDDARVAVETXXXX----XXX 128
P AS+ + I + G I RC+ D A +
Sbjct: 131 FPAASKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIERAWDAVKYAKRPRIHT 190
Query: 129 XIGTSKFLRQYSHGKDMNYIAKSAIEVIEFVKSKGIE-IRFSSEDSFRSDLVDLLNIYKT 187
I TS +Y K + + A ++ F +S G E + FS ED+ RS+ L I
Sbjct: 191 FIATSDIHLEYKLKKTKAEVIEIARSMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGE 250
Query: 188 VDKIGVNRVGIADTVGCANPRQVYELVRTLKS----VVSCDIECHFHNDTGCAIANAYTA 243
V K G + I DTVG P + +L+ LK+ + + I H ND G + AN +
Sbjct: 251 VIKAGATTLNIPDTVGITLPSEFGQLITDLKANTPGIENVVISTHCQNDLGLSTANTLSG 310
Query: 244 LEGGARLIDVAVLGIGERNGITPLGGLMARMIVAAPEYTKSKYKLHKIRDI---ENLIAE 300
GAR ++V + GIGER G L ++ + + R I ++ E
Sbjct: 311 AHAGARQMEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEE 370
Query: 301 AVEVNIPFNNPITGFCAFTHKAGIHAKAILANPSTYEILDPHDFGMKRYIH---FANRLT 357
+ + I G AF H++GIH +L + TYEI+ P + G++R +L+
Sbjct: 371 YTGMQTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLS 430
Query: 358 GWNAIKSRVDQLNLNLT 374
G +A+K R+ +++ NLT
Sbjct: 431 GRHALKDRLTEVHSNLT 447
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.135 0.386
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,100,552
Number of Sequences: 60738
Number of extensions: 969728
Number of successful extensions: 2567
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 2547
Number of HSP's gapped (non-prelim): 7
length of query: 441
length of database: 30,389,216
effective HSP length: 109
effective length of query: 332
effective length of database: 23,768,774
effective search space: 7891232968
effective search space used: 7891232968
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)