ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI1591 good O KOG4400 Posttranslational modification, protein turnover, chaperones E3 ubiquitin ligase interacting with arginine methyltransferase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI1591 567054 566008 -349
(349 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YIL079c [O] KOG4400 E3 ubiquitin ligase interacting with arginin... 324 1e-88
YDL175c [O] KOG4400 E3 ubiquitin ligase interacting with arginin... 316 4e-86
SPBP35G2.08c [O] KOG4400 E3 ubiquitin ligase interacting with ar... 125 1e-28
Hs22045627_2 [O] KOG4400 E3 ubiquitin ligase interacting with ar... 71 2e-12
YNL255c [O] KOG4400 E3 ubiquitin ligase interacting with arginin... 61 2e-09
7294049 [O] KOG4400 E3 ubiquitin ligase interacting with arginin... 59 1e-08
ECU11g0650 [O] KOG4400 E3 ubiquitin ligase interacting with argi... 57 5e-08
At3g43590 [O] KOG4400 E3 ubiquitin ligase interacting with argin... 55 1e-07
7291506 [O] KOG4400 E3 ubiquitin ligase interacting with arginin... 54 3e-07
Hs4827071 [O] KOG4400 E3 ubiquitin ligase interacting with argin... 50 6e-06
SPAC13D6.02c [O] KOG4400 E3 ubiquitin ligase interacting with ar... 49 8e-06
>YIL079c [O] KOG4400 E3 ubiquitin ligase interacting with arginine
methyltransferase
Length = 360
Score = 324 bits (831), Expect = 1e-88
Identities = 165/344 (47%), Positives = 212/344 (60%), Gaps = 13/344 (3%)
Query: 1 MLSEVETADTLPFFKDSSPS-YEITDRDKIIAPSITEVDSDPEKLREIRGQGRYFGIEDG 59
+LSEVE+ DTLP+ KD++P+ + + +K++APSI +VD++PE+LR +RGQGRYFGI D
Sbjct: 4 LLSEVESIDTLPYVKDTTPTGSDSSSFNKLLAPSIEDVDANPEELRTLRGQGRYFGITDY 63
Query: 60 EDG--IVEPLPKCNNCSQRGHLKKNCPHVICSYCGVMDDHYSTHCPKTMRCSHCNEPGHY 117
+ I+E PKCNNCSQRGHLK+NCPHVIC+YCG MDDHYS HCPK + C++CN GHY
Sbjct: 64 DSNGAIMEAEPKCNNCSQRGHLKRNCPHVICTYCGFMDDHYSQHCPKAIICTNCNANGHY 123
Query: 118 RQHCPVKWKRVFCTLCNSSKHSRDRCPSIWRSYCL--KDSKQKRLMPLHLIFCYNCGGKG 175
+ CP KWK+VFCTLCNS +HSR+RCPSIWRSY L KD+ Q +FCYNCG G
Sbjct: 124 KSQCPHKWKKVFCTLCNSKRHSRERCPSIWRSYLLKTKDANQGD-FDFQTVFCYNCGNAG 182
Query: 176 HFGDDCMSARSSRVPNDDGSAFAGNNLPSSVRQDYGRNLKEYKERTANEXXXXXXXXXXX 235
HFGDDC RSSRVPN DGSAF G+NL + +Q Y LK+YK R A++
Sbjct: 183 HFGDDCAERRSSRVPNTDGSAFCGDNLATKFKQHYFNQLKDYK-REASQRQHFDNEHEFN 241
Query: 236 XFNYDGEDXXXXXXXXXXXQPAMDYGDAKPSNFYAPPYNKKSKRSNYNHSNG-SSNAPAP 294
+Y+ D + M + S NK S + Y SN +P
Sbjct: 242 LLDYEYNDDAYDLPGSRTYRDKMKWKGKVQST-----RNKNSSNNRYESSNNRKKKSPFS 296
Query: 295 TVKGKVLPPKPTRSHPLDFPRNPNVNIPRYGSSSYQPSNDRYRK 338
KV K ++HPLDFPR+ N SS + + D + K
Sbjct: 297 AQNYKVTKNKRVQTHPLDFPRSSQNNRTNDYSSQFSYNRDDFPK 340
>YDL175c [O] KOG4400 E3 ubiquitin ligase interacting with arginine
methyltransferase
Length = 344
Score = 316 bits (809), Expect = 4e-86
Identities = 160/356 (44%), Positives = 207/356 (57%), Gaps = 41/356 (11%)
Query: 9 DTLPFFKDSSPSYEITDRDKIIAPSITEVDSDPEKLREIRGQGRYFGI-EDGEDGIVEPL 67
+T PF D++P+ T DK++APSI EV+S+P +LR +RGQGRYFG+ +D +D I E
Sbjct: 4 NTAPFVVDTAPT---TPPDKLVAPSIEEVNSNPNELRALRGQGRYFGVSDDDKDAIKEAA 60
Query: 68 PKCNNCSQRGHLKKNCPHVICSYCGVMDDHYSTHCPKTMRCSHCNEPGHYRQHCPVKWKR 127
PKCNNCSQRGHLKK+CPH+ICSYCG DDHYS HCPK ++CS C+E GHYR CP KWK+
Sbjct: 61 PKCNNCSQRGHLKKDCPHIICSYCGATDDHYSRHCPKAIQCSKCDEVGHYRSQCPHKWKK 120
Query: 128 VFCTLCNSSKHSRDRCPSIWRSYCLKDSKQK---RLMPLHLIFCYNCGGKGHFGDDCMSA 184
V CTLC S KHS++RCPSIWR+Y L D +K +++P H I+CYNCGGKGHFGDDC
Sbjct: 121 VQCTLCKSKKHSKERCPSIWRAYILVDDNEKAKPKVLPFHTIYCYNCGGKGHFGDDCKEK 180
Query: 185 RSSRVPNDDGSAFAGNNLPSSVRQDYGRNLKEYKERTANEXXXXXXXXXXXXFNYDGEDX 244
RSSRVPN+DGSAF G+NL ++Q+Y Y+ N ++ D
Sbjct: 181 RSSRVPNEDGSAFTGSNLSVELKQEY------YRHMNRNSDENEDYQFSESIYDEDPLPR 234
Query: 245 XXXXXXXXXXQPAMDYGDAKPSNFYAPPYNKKSKRSNYNHSNGSSNAPAPTVKGKVLPPK 304
+ SNF+ PPY K SN PT++G+ L
Sbjct: 235 PSHKRHSQNDHSHSGRNKRRASNFHPPPYQK-------------SNVIQPTIRGETLSLN 281
Query: 305 PTRSHPLDFPRNPNVNI---------PRYGSSSYQPSN-----DRYRKYNSYSGFK 346
S + +N VN+ RY S+Y +N YR YNSY ++
Sbjct: 282 NNISKNSRY-QNTKVNVSSISENMYGSRYNPSTYVDNNSISNSSNYRNYNSYQPYR 336
>SPBP35G2.08c [O] KOG4400 E3 ubiquitin ligase interacting with arginine
methyltransferase
Length = 313
Score = 125 bits (313), Expect = 1e-28
Identities = 61/157 (38%), Positives = 80/157 (50%), Gaps = 10/157 (6%)
Query: 50 QGRYFGIEDGEDGIVEPLPKCNNCSQRGHLKKNCPHVICSYCGVMDDHYSTHCPKTMRCS 109
+GRYFG + E + C+NC GH+ K+CPHV+C+ CG +DDH S CP T +C
Sbjct: 75 RGRYFGSDPSESIV------CHNCKGNGHISKDCPHVLCTTCGAIDDHISVRCPWTKKCM 128
Query: 110 HCNEPGHYRQHCPVKWKR--VFCTLCNSSKHSRDRCPSIWRSYCLKDSKQKRLMPLHLIF 167
+C GH C KR C C++ H+ CP IWR Y K+ + + F
Sbjct: 129 NCGLLGHIAARCSEPRKRGPRVCRTCHTDTHTSSTCPLIWRYYVEKEHPVRIDVSEVRKF 188
Query: 168 CYNCGGKGHFGDDCMSARSSRVPNDDGSAFAGNNLPS 204
CYNC HFGDDC S P + +AF N PS
Sbjct: 189 CYNCASDEHFGDDCTLPSRSNYP--ESTAFCEANCPS 223
>Hs22045627_2 [O] KOG4400 E3 ubiquitin ligase interacting with arginine
methyltransferase
Length = 356
Score = 71.2 bits (173), Expect = 2e-12
Identities = 44/127 (34%), Positives = 56/127 (43%), Gaps = 21/127 (16%)
Query: 70 CNNCSQRGHLKKNCP-------HVICSYCGVMDDHYSTHCPKTMRCSHCNEPGHYRQHCP 122
C NC +RGHL KNCP +CS G H CP + C +C P C
Sbjct: 56 CRNCDKRGHLSKNCPLPRKVRRCFLCSRRG----HLLYSCPAPL-CEYCPVPKMLDHSCL 110
Query: 123 VK--WKRVFCTLCNSSKHSRDRCPSIWRSYCL--KDSKQKR----LMPLHLIFCYNCGGK 174
+ W + C C+ H D C IWR Y L K K+ P L +CY+C K
Sbjct: 111 FRHSWDKQ-CDRCHMLGHYTDACTEIWRQYHLTTKPGPPKKPKTPSRPSALAYCYHCAQK 169
Query: 175 GHFGDDC 181
GH+G +C
Sbjct: 170 GHYGHEC 176
>YNL255c [O] KOG4400 E3 ubiquitin ligase interacting with arginine
methyltransferase
Length = 153
Score = 61.2 bits (147), Expect = 2e-09
Identities = 36/118 (30%), Positives = 48/118 (40%), Gaps = 20/118 (16%)
Query: 70 CNNCSQRGHLKKNCPHVICSYCGVMDDHYSTHC--PKTM---RCSHCNEPGHYRQHCPVK 124
C C + GHL ++C Y H T C P+T+ +C +C E GH R C V+
Sbjct: 6 CYVCGKIGHLAEDCDSERLCYNCNKPGHVQTDCTMPRTVEFKQCYNCGETGHVRSECTVQ 65
Query: 125 WKRVFCTLCNSSKHSRDRCPSIWRSYCLKDSKQKRLMPLHLIFCYNCGGKGHFGDDCM 182
C CN + H CP + K+ + CY CGG H DCM
Sbjct: 66 R----CFNCNQTGHISRECP-----------EPKKTSRFSKVSCYKCGGPNHMAKDCM 108
Score = 58.2 bits (139), Expect = 2e-08
Identities = 39/137 (28%), Positives = 58/137 (41%), Gaps = 29/137 (21%)
Query: 70 CNNCSQRGHLKKNC--PHVI----CSYCGVMDDHYSTHCPKTMRCSHCNEPGHYRQHCPV 123
C NC++ GH++ +C P + C CG H + C RC +CN+ GH + CP
Sbjct: 25 CYNCNKPGHVQTDCTMPRTVEFKQCYNCGETG-HVRSECT-VQRCFNCNQTGHISRECPE 82
Query: 124 -----KWKRVFCTLCNSSKH-SRDRCPSIWRSYCLKDSKQKRLMPLHLIFCYNCGGKGHF 177
++ +V C C H ++D C+K+ L CY CG GH
Sbjct: 83 PKKTSRFSKVSCYKCGGPNHMAKD---------CMKEDGISGLK------CYTCGQAGHM 127
Query: 178 GDDCMSARSSRVPNDDG 194
DC + R N+ G
Sbjct: 128 SRDCQNDRLCYNCNETG 144
>7294049 [O] KOG4400 E3 ubiquitin ligase interacting with arginine
methyltransferase
Length = 1700
Score = 58.5 bits (140), Expect = 1e-08
Identities = 36/119 (30%), Positives = 47/119 (39%), Gaps = 12/119 (10%)
Query: 69 KCNNCSQRGHLKKNCPH----VICSYCGVMDDHYSTHCPKTMRCSHCNEPGH-YRQHCP- 122
KC+NC + GH++ CP ++C CG M H CP + C C Y Q C
Sbjct: 818 KCSNCFEMGHVRSKCPRPRKPLVCFICGTM-GHAEPRCPNAI-CFGCGSKQEIYVQQCNK 875
Query: 123 -VKWKRVFCTLCNSSKHSRDRCPSIWRSYCLKDSKQKRL---MPLHLIFCYNCGGKGHF 177
R+ C LC H D CP WR Y L + + C C G+ F
Sbjct: 876 CSFHSRLVCQLCKMRGHGVDHCPDKWRRYHSTTRSNTELDSRVQYRSVQCSYCAGRHSF 934
>ECU11g0650 [O] KOG4400 E3 ubiquitin ligase interacting with arginine
methyltransferase
Length = 220
Score = 56.6 bits (135), Expect = 5e-08
Identities = 37/119 (31%), Positives = 47/119 (39%), Gaps = 12/119 (10%)
Query: 70 CNNCSQRGHLKKNCPHV----ICSYCGVMDDHYSTHCPKTMRCSHCNEPGHYRQHC--PV 123
C C + GH + CP +C C D H S CP + C C GH C P
Sbjct: 83 CFRCGETGHGIRECPKAPGKDVCELCS-WDGHRSLCCPYRL-CPRCGRCGHSPDDCLEPE 140
Query: 124 KWKRV-FCTLCNSSKHSRDRCPSIWRSYCLKDSKQKRLMPLHLIFCYNCGGKGHFGDDC 181
R C C + HS + CP W+ Y L + KR + C C + HF DC
Sbjct: 141 SLDRSKMCEACPTGFHSTEDCPRTWKRYKLNRNTSKRTI---YKACPICLSRKHFIGDC 196
>At3g43590 [O] KOG4400 E3 ubiquitin ligase interacting with arginine
methyltransferase
Length = 551
Score = 55.5 bits (132), Expect = 1e-07
Identities = 51/184 (27%), Positives = 69/184 (36%), Gaps = 31/184 (16%)
Query: 35 TEVDSDPEKLREIRGQGRYFGIEDGEDGIVEPLPKCNNCSQRGHLKKNCPHVI-----CS 89
TE + L+++ RYF D G V C +C ++GH NCP C
Sbjct: 139 TEETASNLVLKKLLRGARYFDPPDA--GWVS----CYSCGEQGHTSFNCPTPTKRRKPCF 192
Query: 90 YCGVMDDHYSTHCPKTMRCSHCNEPGHYRQHCPVKWKR----VFCTLCNSSKHSRDRCPS 145
CG ++ H + C K C C + GH + CP K+K C C H C
Sbjct: 193 ICGSLE-HGAKQCSKGHDCYICKKTGHRAKDCPDKYKNGSKGAVCLRCGDFGHDMILCKY 251
Query: 146 IWRSYCLKDSKQKRLMPL-HL------------IFCYNCGGKGHFGDDCMSARSSRVPND 192
+ LKD + HL + CY CG GH G C R N+
Sbjct: 252 EYSKEDLKDVQCYICKSFGHLCCVEPGNSLSWAVSCYRCGQLGHSGLAC--GRHYEESNE 309
Query: 193 DGSA 196
+ SA
Sbjct: 310 NDSA 313
>7291506 [O] KOG4400 E3 ubiquitin ligase interacting with arginine
methyltransferase
Length = 124
Score = 54.3 bits (129), Expect = 3e-07
Identities = 34/122 (27%), Positives = 46/122 (36%), Gaps = 28/122 (22%)
Query: 69 KCNNCSQRGHLKKNCPHVI--CSYCGVMDDHYSTHCPK--TMRCSHCNEPGHYRQHCPVK 124
KC C+Q GH + CP C C + H S C + C CN+ GH+ ++CP
Sbjct: 15 KCYKCNQFGHFARACPEEAERCYRCNGI-GHISKDCTQADNPTCYRCNKTGHWVRNCPEA 73
Query: 125 WK-----RVFCTLCNSSKHSRDRCPSIWRSYCLKDSKQKRLMPLHLIFCYNCGGKGHFGD 179
V C CN + H CP ++ CY CG GH
Sbjct: 74 VNERGPTNVSCYKCNRTGHISKNCPETSKT------------------CYGCGKSGHLRR 115
Query: 180 DC 181
+C
Sbjct: 116 EC 117
>Hs4827071 [O] KOG4400 E3 ubiquitin ligase interacting with arginine
methyltransferase
Length = 177
Score = 49.7 bits (117), Expect = 6e-06
Identities = 39/147 (26%), Positives = 57/147 (38%), Gaps = 12/147 (8%)
Query: 45 REIRGQGRY-FGIEDGEDGIVEPLPK-CNNCSQRGHLKKNCP--HVICSYCGVMDDHYST 100
R +R +GR F + G + LP C C + GHL K+C C CG H +
Sbjct: 27 RGMRSRGRGGFTSDRGFQFVSSSLPDICYRCGESGHLAKDCDLQEDACYNCG-RGGHIAK 85
Query: 101 HCPKTMR-----CSHCNEPGHYRQHCPVKWKRVFCTLCNSSKHSRDRCPSIWRSYCLKDS 155
C + R C +C +PGH + C ++ C C H + C + C +
Sbjct: 86 DCKEPKREREQCCYNCGKPGHLARDCDHADEQK-CYSCGEFGHIQKDCTKVKCYRCGETG 144
Query: 156 KQK-RLMPLHLIFCYNCGGKGHFGDDC 181
+ CY CG GH +C
Sbjct: 145 HVAINCSKTSEVNCYRCGESGHLAREC 171
>SPAC13D6.02c [O] KOG4400 E3 ubiquitin ligase interacting with arginine
methyltransferase
Length = 179
Score = 49.3 bits (116), Expect = 8e-06
Identities = 30/116 (25%), Positives = 38/116 (31%), Gaps = 32/116 (27%)
Query: 66 PLPKCNNCSQRGHLKKNCPHVICSYCGVMDDHYSTHCPKTMRCSHCNEPGHYRQHCPVKW 125
P P+C NC + GH + C K C +CN+ GH C
Sbjct: 15 PGPRCYNCGENGHQARECT-------------------KGSICYNCNQTGHKASECTEPQ 55
Query: 126 KRVFCTLCNSSKHSRDRCPSIWRSYCLKDSKQKRLMPLHLIFCYNCGGKGHFGDDC 181
+ C C ++ H CPS P CY CG GH DC
Sbjct: 56 QEKTCYACGTAGHLVRDCPS-------------SPNPRQGAECYKCGRVGHIARDC 98
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.135 0.434
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,956,240
Number of Sequences: 60738
Number of extensions: 1230926
Number of successful extensions: 4427
Number of sequences better than 1.0e-05: 11
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 4306
Number of HSP's gapped (non-prelim): 55
length of query: 349
length of database: 30,389,216
effective HSP length: 107
effective length of query: 242
effective length of database: 23,890,250
effective search space: 5781440500
effective search space used: 5781440500
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)