ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI1609 good R KOG1541 General function prediction only Predicted protein carboxyl methylase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI1609 571896 572696 267
(267 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YCR047c [R] KOG1541 Predicted protein carboxyl methylase 419 e-117
SPAC26A3.06 [R] KOG1541 Predicted protein carboxyl methylase 277 1e-74
7298980 [R] KOG1541 Predicted protein carboxyl methylase 266 2e-71
At5g57280 [R] KOG1541 Predicted protein carboxyl methylase 259 3e-69
CE01171 [R] KOG1541 Predicted protein carboxyl methylase 230 1e-60
Hs8923714 [R] KOG1541 Predicted protein carboxyl methylase 193 2e-49
ECU05g0950 [R] KOG1541 Predicted protein carboxyl methylase 177 1e-44
>YCR047c [R] KOG1541 Predicted protein carboxyl methylase
Length = 275
Score = 419 bits (1077), Expect = e-117
Identities = 213/275 (77%), Positives = 242/275 (87%), Gaps = 8/275 (2%)
Query: 1 MSRPEDLAPPEVFYNDSESKKYSGSTRVQHIQAKMTLRALELLNLPHTSYILDIGCGSGL 60
MSRPE+LAPPE+FYNDSE+ KY+GSTRVQHIQAKMTLRALELLNL S+ILDIGCGSGL
Sbjct: 1 MSRPEELAPPEIFYNDSEAHKYTGSTRVQHIQAKMTLRALELLNLQPCSFILDIGCGSGL 60
Query: 61 SGEIITEEG-HIWCGLDISPSMLAEALEREVEGDLMLHDIGQGIPFRAGVFDAAISISVI 119
SGEI+T+EG H+WCGLDISPSMLA L RE+EGDLML D+G GIPFRAG FDAAISIS I
Sbjct: 61 SGEILTQEGDHVWCGLDISPSMLATGLSRELEGDLMLQDMGTGIPFRAGSFDAAISISAI 120
Query: 120 QWLCNADTSYNDPKRRLMRFFNTLFASLKKGGKFVAQFYPKNDDQIDQILGAAKVAGFSG 179
QWLCNADTSYNDPK+RLMRFFNTL+A+LKKGGKFVAQFYPKNDDQ+D IL +AKVAGFSG
Sbjct: 121 QWLCNADTSYNDPKQRLMRFFNTLYAALKKGGKFVAQFYPKNDDQVDDILQSAKVAGFSG 180
Query: 180 GLVIDDPESKKNKKYYLVLSSGSTEQ-NEQVNLDGVKMDAD-LMVKRERKKKL-----VE 232
GLV+DDPESKKNKKYYLVLSSG+ Q EQVNLDGV MD + + +K++ +++L E
Sbjct: 181 GLVVDDPESKKNKKYYLVLSSGAPPQGEEQVNLDGVTMDEENVNLKKQLRQRLKGGKDKE 240
Query: 233 SSKDYINRKKEVMKKRGRKVALDSKFTGRKRRPRF 267
S+K +I RKKE+MK+RGRKVA DSKFTGRKRR RF
Sbjct: 241 SAKSFILRKKELMKRRGRKVAKDSKFTGRKRRHRF 275
>SPAC26A3.06 [R] KOG1541 Predicted protein carboxyl methylase
Length = 268
Score = 277 bits (708), Expect = 1e-74
Identities = 143/271 (52%), Positives = 196/271 (71%), Gaps = 7/271 (2%)
Query: 1 MSRPEDLAPPEVFYNDSESKKYSGSTRVQHIQAKMTLRALELLNLPHTSYILDIGCGSGL 60
MSRPE +APPE+FYND E+ KYS +TR+Q IQ +M+ RALELL+ S+ILDIGCGSG+
Sbjct: 1 MSRPEHIAPPEIFYNDVEAGKYSTNTRIQSIQTEMSERALELLDAEGPSFILDIGCGSGI 60
Query: 61 SGEIITEEGHIWCGLDISPSMLAEALE-REVEGDLMLHDIGQGIPFRAGVFDAAISISVI 119
S +I +GH+ G+DISPSML+ ALE +E+EGDL+L D+G G+PFR G FD ISIS I
Sbjct: 61 STQIGESQGHVVVGMDISPSMLSVALESQEIEGDLLLCDMGTGVPFRPGTFDGVISISAI 120
Query: 120 QWLCNADTSYNDPKRRLMRFFNTLFASLKKGGKFVAQFYPKNDDQIDQILGAAKVAGFSG 179
QWL NAD + N P+RRL RFF TL+ S+K+GG+ V Q+YP+ + I+ A+ AGF+G
Sbjct: 121 QWLLNADKTCNVPQRRLNRFFQTLYISMKRGGRAVMQYYPETEKSQQMIMDTARKAGFAG 180
Query: 180 GLVIDDPESKKNKKYYLVLSSGSTEQNEQVNLDGVKMDADLMVKRER--KKKLVESSKDY 237
G+V+D PESK+ KKYYLVL +G T +++ + +D + ++R KKK S+++Y
Sbjct: 181 GIVVDHPESKRQKKYYLVLQAGGT---RTLDISSMTLDQEGTNAKQRKLKKKQDMSTREY 237
Query: 238 INRKKEVMKKRGR-KVALDSKFTGRKRRPRF 267
I KKE+ +KRGR V DSK++GR+R+ F
Sbjct: 238 IIHKKELNRKRGRLHVPKDSKYSGRRRKAAF 268
>7298980 [R] KOG1541 Predicted protein carboxyl methylase
Length = 276
Score = 266 bits (680), Expect = 2e-71
Identities = 142/278 (51%), Positives = 187/278 (67%), Gaps = 18/278 (6%)
Query: 3 RPEDLAPPEVFYNDSESKKYSGSTRVQHIQAKMTLRALELLNLP---HTSYILDIGCGSG 59
RPE APPE+FYND E+KKYS +TR+ IQ +M RALELL LP + ILDIGCGSG
Sbjct: 4 RPEHSAPPEIFYNDDEAKKYSTNTRIIEIQVEMAERALELLALPDDDESRLILDIGCGSG 63
Query: 60 LSGEIITEEGHIWCGLDISPSMLAEALEREVEGDLMLHDIGQGIPFRAGVFDAAISISVI 119
LSG ++ + H+W G+DIS SML A+EREV GD++L D+G+G+PF+ G FD AISIS +
Sbjct: 64 LSGSVLEDSEHMWIGIDISKSMLDIAVEREVAGDVILGDMGEGMPFKPGTFDGAISISAL 123
Query: 120 QWLCNADTSYNDPKRRLMRFFNTLFASLKKGGKFVAQFYPKNDDQIDQILGAAKVAGFSG 179
QWLCNAD SY++P +RL++FF TLF+ L + + V QFYP+N DQI+ + A AGF G
Sbjct: 124 QWLCNADKSYHNPHKRLLKFFTTLFSCLTRTARAVFQFYPENSDQIEMVTSQAMKAGFYG 183
Query: 180 GLVIDDPESKKNKKYYLVLSS----------GSTEQNEQVNLDGVKMDADLMVKRERKKK 229
GLV+D P S K KKYYLVL + GS E+ +VN K DA RE + K
Sbjct: 184 GLVVDYPNSAKAKKYYLVLMTGGSAELPQALGSPEEERRVNYI-KKRDA----CREARGK 238
Query: 230 LVESSKDYINRKKEVMKKRGRKVALDSKFTGRKRRPRF 267
+ S+D+I KKE +++G + D+K+T RKR +F
Sbjct: 239 APKKSRDWILAKKERRRRQGLETRPDTKYTARKRSGKF 276
>At5g57280 [R] KOG1541 Predicted protein carboxyl methylase
Length = 289
Score = 259 bits (662), Expect = 3e-69
Identities = 139/287 (48%), Positives = 191/287 (66%), Gaps = 21/287 (7%)
Query: 2 SRPEDLAPPEVFYNDSESKKYSGSTRVQHIQAKMTLRALELLNLPHTS---YILDIGCGS 58
+RPE LAPPE+FY+D+E++KY+ S+R+ IQAK++ RALELL LP ++LDIGCGS
Sbjct: 3 NRPELLAPPEIFYDDTEARKYTSSSRIVEIQAKLSERALELLALPEDGVPRFLLDIGCGS 62
Query: 59 GLSGEIITEEGHIWCGLDISPSMLAEALEREVEGDLMLHDIGQGIPFRAGVFDAAISISV 118
GLSGE ++E+GH W GLDIS SML A+EREVEGDL+L D+GQG+ R+GV D AISIS
Sbjct: 63 GLSGETLSEDGHHWIGLDISASMLHVAVEREVEGDLLLGDMGQGLGLRSGVIDGAISISA 122
Query: 119 IQWLCNADTSYNDPKRRLMRFFNTLFASLKKGGKFVAQFYPKNDDQIDQILGAAKVAGFS 178
+QWLCNAD S ++P+ RL FF +L+ L +G + V Q YP+N Q + IL A AGF
Sbjct: 123 VQWLCNADKSSHEPRLRLKAFFGSLYRCLSRGARAVFQVYPENIAQRELILRQALQAGFG 182
Query: 179 GGLVIDDPESKKNKKYYLVLSSGSTEQ-------------NEQVNLDGVKMDADLMVKRE 225
GGLV+D P S K +K +LVL+ G+ + +E N D + + + R
Sbjct: 183 GGLVVDYPHSTKKRKEFLVLTCGTVQTSIQTSKNEYDESCSEDDNSDDEESEEVGVSDRN 242
Query: 226 RKKKLVESS-----KDYINRKKEVMKKRGRKVALDSKFTGRKRRPRF 267
R +K ++ ++++ RKKE +++G+ V DSKFT RKRR RF
Sbjct: 243 RPRKRQRTNTKVKGREWVLRKKEQSRRKGKNVPADSKFTSRKRRTRF 289
>CE01171 [R] KOG1541 Predicted protein carboxyl methylase
Length = 283
Score = 230 bits (587), Expect = 1e-60
Identities = 119/277 (42%), Positives = 178/277 (63%), Gaps = 12/277 (4%)
Query: 3 RPEDLAPPEVFYNDSESKKYSGSTRVQHIQAKMTLRALELLNLPH--TSYILDIGCGSGL 60
+PE PP+++YN++E+ KY+ ++ + IQ +M RALELL LP + ++LDIGCG+G+
Sbjct: 7 KPEHTGPPDLYYNETEAAKYASNSHITAIQHEMAERALELLALPEGKSGFLLDIGCGTGM 66
Query: 61 SGEIITEEGHIWCGLDISPSMLAEALERE--VEGDLMLHDIGQGIPFRAGVFDAAISISV 118
S E+I + GH++ G+D+S ML A + E GD + D+G G+PFR G FD AISIS
Sbjct: 67 SSEVILDAGHMFVGVDVSRPMLEIARQDEDLESGDFIHQDMGLGMPFRPGSFDGAISISA 126
Query: 119 IQWLCNADTSYNDPKRRLMRFFNTLFASLKKGGKFVAQFYPKNDDQIDQILGAAKVAGFS 178
IQWLC+A+ S +P++RL+ FF +L+ L +G + V QFYP+ND+Q D I+G A AGF+
Sbjct: 127 IQWLCHANASDENPRKRLLFFFQSLYGCLGRGSRAVFQFYPENDEQCDLIMGQAHKAGFN 186
Query: 179 GGLVIDDPESKKNKKYYLVLSSGS--------TEQNEQVNLDGVKMDADLMVKRERKKKL 230
GGLV+D PE+ K KK YLVL +G TE E+ + + +K+
Sbjct: 187 GGLVVDFPEAAKRKKVYLVLMTGGVVQLPQALTEDGEESRTQIDNAGRRFVWNSRKNEKV 246
Query: 231 VESSKDYINRKKEVMKKRGRKVALDSKFTGRKRRPRF 267
+ SK +I K++ K+GR V +SK++GRKR+ +F
Sbjct: 247 AKGSKAWIEAKRQRQIKQGRDVRHESKYSGRKRKTKF 283
>Hs8923714 [R] KOG1541 Predicted protein carboxyl methylase
Length = 220
Score = 193 bits (490), Expect = 2e-49
Identities = 95/181 (52%), Positives = 128/181 (70%), Gaps = 3/181 (1%)
Query: 3 RPEDLAPPEVFYNDSESKKYSGSTRVQHIQAKMTLRALELLNLPHTS--YILDIGCGSGL 60
RPE PPE+FY+++E++KY ++R+ IQ +M RALELL LP Y+LDIGCG+GL
Sbjct: 7 RPEHGGPPELFYDETEARKYVRNSRMIDIQTRMAGRALELLYLPENKPCYLLDIGCGTGL 66
Query: 61 SGEIITEEGHIWCGLDISPSMLAEALEREVEGDLMLHDIGQGIPFRAGVFDAAISISVIQ 120
SG +++EGH W GLDISP+ML EA++RE+EGDL+L D+GQGIPF+ G FD ISIS +Q
Sbjct: 67 SGSYLSDEGHYWVGLDISPAMLDEAVDREIEGDLLLGDMGQGIPFKPGTFDGCISISAVQ 126
Query: 121 WLCNADTSYNDPKRRLMRFFNTLFASLKKGGKFVAQFYPKNDDQID-QILGAAKVAGFSG 179
WLCNA+ +P +RL FF +LF+ L +G + V Q YP+N +Q+ G A G S
Sbjct: 127 WLCNANKKSENPAKRLYCFFASLFSVLVRGSRAVLQLYPENSEQVSPSATGCAGELGDST 186
Query: 180 G 180
G
Sbjct: 187 G 187
>ECU05g0950 [R] KOG1541 Predicted protein carboxyl methylase
Length = 247
Score = 177 bits (449), Expect = 1e-44
Identities = 98/266 (36%), Positives = 155/266 (57%), Gaps = 19/266 (7%)
Query: 1 MSRPEDLAPPEVFYNDSESKKYSGSTRVQHIQAKMTLRALELLNLPHTSYILDIGCGSGL 60
M PE P E++Y++ +S +Y+ ++R+ +IQ ++T R LELL+ +LD+GCGSGL
Sbjct: 1 MGIPELSGPAELYYDEEQSLRYTQNSRIVYIQKELTQRCLELLDAKDGGLVLDVGCGSGL 60
Query: 61 SGEIITEEGHIWCGLDISPSMLAEALEREVEGDLMLHDIGQGIPFRAGVFDAAISISVIQ 120
SG +++E G+ W G+DIS ML +ER + D+G+G+ F+ G FD IS+S +Q
Sbjct: 61 SGSVLSESGYPWIGVDISMEMLKLGMERMEGAGYIRMDMGEGLQFQPGTFDGVISVSAVQ 120
Query: 121 WLCNADTSYNDPKRRLMRFFNTLFASLKKGGKFVAQFYPKNDDQIDQILGAAKVAGFSGG 180
WL ++ +S + P RR+ FF TL++ K + V QFY K+ QI+ + A AGF GG
Sbjct: 121 WLFHSYSSGDHPVRRIRTFFTTLYSVCKPDARCVLQFYLKSQGQIEMLKSEAIRAGFGGG 180
Query: 181 LVIDDPESKKNKKYYLVLSSGSTEQNEQVNLDGVKMDADLMVKRERKKKLVESSKDYINR 240
+ ID+ E +N K +LVL+ + +K+E+K+ D I R
Sbjct: 181 VQIDN-EGTRNVKSFLVLT----------------LSGGARIKKEKKRG--TGRIDRILR 221
Query: 241 KKEVMKKRGRKVALDSKFTGRKRRPR 266
+KE ++ RG V D+K+TG++R R
Sbjct: 222 RKEKLRLRGVCVPRDTKYTGKRRSKR 247
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.136 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,114,958
Number of Sequences: 60738
Number of extensions: 682014
Number of successful extensions: 2494
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2472
Number of HSP's gapped (non-prelim): 7
length of query: 267
length of database: 30,389,216
effective HSP length: 104
effective length of query: 163
effective length of database: 24,072,464
effective search space: 3923811632
effective search space used: 3923811632
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)