ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI1622 good O KOG1153 Posttranslational modification, protein turnover, chaperones Subtilisin-related protease/Vacuolar protease B
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI1622 576687 578210 508
(508 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YCR045c [O] KOG1153 Subtilisin-related protease/Vacuolar protease B 296 6e-80
YOR003w [O] KOG1153 Subtilisin-related protease/Vacuolar protease B 177 3e-44
SPAC1006.01 [O] KOG1153 Subtilisin-related protease/Vacuolar pro... 168 2e-41
SPAC4A8.04 [O] KOG1153 Subtilisin-related protease/Vacuolar prot... 166 8e-41
YEL060c [O] KOG1153 Subtilisin-related protease/Vacuolar protease B 159 7e-39
ECU01g1130 [O] KOG1153 Subtilisin-related protease/Vacuolar prot... 112 1e-24
ECU03g1180 [O] KOG1153 Subtilisin-related protease/Vacuolar prot... 66 1e-10
Hs4506775 [O] KOG4266 Subtilisin kexin isozyme-1/site 1 protease... 64 5e-10
At5g19660 [O] KOG4266 Subtilisin kexin isozyme-1/site 1 protease... 53 9e-07
>YCR045c [O] KOG1153 Subtilisin-related protease/Vacuolar protease B
Length = 491
Score = 296 bits (757), Expect = 6e-80
Identities = 168/467 (35%), Positives = 246/467 (51%), Gaps = 34/467 (7%)
Query: 22 EYLVSLKTPRALRYVFNFKIGGSSVKELIESKIHREFSFGKFNAISVDIPPVLADKLKDV 81
EYLV K P A + + S ++ I++KI ++FS G F +++++ L +KLK
Sbjct: 19 EYLVRFKNPTAFQQFTSNS--NRSWRQFIDNKIEKKFSIGSFRGVTMNLSKNLVNKLKKS 76
Query: 82 PFISDVVANVKISLFDEFPQIASGLNNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 141
P ++D+V N + F+ +N+
Sbjct: 77 PLVADIVPNFRFEAFE-----GDSVNSAESSYTFNATAKYSYEDVEEEQNITY------- 124
Query: 142 XXXXXXXXXXISQSRAPRHLARISRRAQLP--VIEKDDTSSGLKYHYHEEHQGEGVFVYV 199
Q APRHLARISR QLP V +KD S Y+Y ++QG+ V Y+
Sbjct: 125 ------------QPDAPRHLARISRHYQLPFDVGDKDRYKSWFNYYYEHDYQGQDVNAYI 172
Query: 200 LDSGVNHDHPDFEGRAMKGIDTTGEGSGDLVGHGTHVAGLIGSKTYGVAKKVNIVEVKVL 259
+D+G+ DHP+FE R ++GID T EG GD GHGTHVAGL+GSKTYG AK+VN+VEVKVL
Sbjct: 173 MDTGIFADHPEFEDRVIQGIDLTKEGFGDQNGHGTHVAGLVGSKTYGAAKRVNLVEVKVL 232
Query: 260 DSMGKGTVTNIIEGLEYVVNQCIGKKR----KCVINMSLGAPGTHSILDKXXXXXXXXXX 315
G G +N++ GLE++V C R KCV N+SLG+ I++
Sbjct: 233 GKDGSGEASNVLSGLEFIVEHCTKVSRPQGKKCVANLSLGS-FRSPIINMAVEGAIEEGI 291
Query: 316 XXXXXXXXXXXXXCWYSPAKVRKAITVAAMDDRTDLMAPFSNWGACIDIWAPGVNVKSLS 375
W SPA ITV A DD D +A FSNWG C++I+APGV ++SLS
Sbjct: 292 VFVAAAGNFNLDAYWASPASAENVITVGAFDDHIDTIAKFSNWGPCVNIFAPGVEIESLS 351
Query: 376 NKG-RGTFVLSGTSMASPIVAGAAAIYLDQGADPAEVRKILSKTATRGVFKKRAIAVRPR 434
+ T +LSGTSM++PIV G AAI L +G +P + + + +TR VF +R + +P
Sbjct: 352 HLNYNDTLILSGTSMSTPIVTGVAAILLSKGIEPEMIAQEIEYLSTRNVFHRRTLFFKPS 411
Query: 435 TPNKVLYTGNNQEDDEFAYAVYPMIDEIKFMDQLKQATSRVKPPSGE 481
TPN++LY G ++ DD + +P ++ +L++ + ++ P E
Sbjct: 412 TPNQILYNGVDKLDDPYDDETFPRLNIEAIAKELEEYNATLQTPMSE 458
>YOR003w [O] KOG1153 Subtilisin-related protease/Vacuolar protease B
Length = 478
Score = 177 bits (450), Expect = 3e-44
Identities = 122/321 (38%), Positives = 169/321 (52%), Gaps = 39/321 (12%)
Query: 154 QSRAPRHLARISRRAQLPVIEKDDTSSGLKYHYHEEHQGEGVFVYVLDSGVNHDHPDFEG 213
Q AP L R+S R EK L+Y Y E+ G+GV YVLD+G++ +H DFEG
Sbjct: 173 QEEAPWGLHRVSHR------EKPKYGQDLEYLY-EDAAGKGVTSYVLDTGIDTEHEDFEG 225
Query: 214 RAMKG-IDTTGEGSGDLVGHGTHVAGLIGSKTYGVAKKVNIVEVKVLDSMGKGTVTNIIE 272
RA G + + + DL GHGTH AG+IGSK +GVAK IV VKVL S G+GTV+++I+
Sbjct: 226 RAEWGAVIPANDEASDLNGHGTHCAGIIGSKHFGVAKNTKIVAVKVLRSNGEGTVSDVIK 285
Query: 273 GLEYVVNQCIGKKRK-------CVINMSLGAPGTHSILDKXXXXXXXXXXXXXXXXXXXX 325
G+EYV + I +K N+SLG+ + + ++
Sbjct: 286 GIEYVTKEHIESSKKKNKEFKGSTANLSLGSSKSLA-MEMAVNAAVDSGVHFAIAAGNED 344
Query: 326 XXXCWYSPAKVRKAITVAA---MDDRTDLMAPFSNWGACIDIWAPGVNVKSLSNKGR-GT 381
C SPA K+ITV A DDR A FSNWG C+D++APG+N+ S R T
Sbjct: 345 EDACLSSPAGAEKSITVGASTFSDDR----AFFSNWGTCVDVFAPGINIMSTYIGSRNAT 400
Query: 382 FVLSGTSMASPIVAGAAAIYL-----------DQGADPAEVRKILSKTATRGVFKKRAIA 430
LSGTSMASP VAG + +L + P E+++ + K +T+GV
Sbjct: 401 LSLSGTSMASPHVAGILSYFLSLQPAPDSEFFNDAPSPQELKEKVLKFSTQGVLGD---- 456
Query: 431 VRPRTPNKVLYTGNNQEDDEF 451
+ TPNK++Y G ++ D F
Sbjct: 457 IGDDTPNKLIYNGGGKKLDGF 477
>SPAC1006.01 [O] KOG1153 Subtilisin-related protease/Vacuolar protease B
Length = 451
Score = 168 bits (425), Expect = 2e-41
Identities = 118/304 (38%), Positives = 161/304 (52%), Gaps = 27/304 (8%)
Query: 154 QSRAPRHLARISRRAQLPVIEKDDTSSGLKYHYHEEHQGEGVFVYVLDSGVNHDHPDFEG 213
Q+ AP LAR+S R +L +Y Y+E GEGV YV+D+G+N +H DF+G
Sbjct: 165 QTFAPWGLARVSHRKKLGFFTMT------RYQYNET-AGEGVTAYVIDTGINIEHQDFQG 217
Query: 214 RAMKGIDT-TGEGSGDLVGHGTHVAGLIGSKTYGVAKKVNIVEVKVLDSMGKGTVTNIIE 272
RA G TGEG D GHGTHVAG I KT+GV+K +V VKV+ + G GTV++II+
Sbjct: 218 RATWGATIPTGEGEVDDHGHGTHVAGTIAGKTFGVSKNAKLVAVKVMRADGTGTVSDIIK 277
Query: 273 GLEYVVNQCIGKKRK----CVINMSLGAPGTHSILDKXXXXXXXXXXXXXXXXXXXXXXX 328
G+E+ Q KK K V+NMS+G + + LD
Sbjct: 278 GIEFAFKQ--SKKDKESIASVVNMSIGGDAS-TALDLAVNAAIAGGLFFAVAAGNDAEDA 334
Query: 329 CWYSPAKVRKAITVAAMDDRTDLMAPFSNWGACIDIWAPGVNVKS-LSNKGRGTFVLSGT 387
C SPA+V A+TV A D +A FSN G+C+DI+APG + S R + +LSGT
Sbjct: 335 CGTSPARVSNAMTVGA-STWNDQIASFSNIGSCVDIFAPGSLILSDWIGSNRASMILSGT 393
Query: 388 SMASPIVAGAAAIYLDQGAD----PAEVRKILSKTATRGVFKKRAIAVRPRTPNKVLYTG 443
SMASP VAG AA ++ P E++K + K A + + + TPN + +
Sbjct: 394 SMASPHVAGLAAYFISLDPSLANHPVELKKYMLKFALKDLLN----GIPEDTPNVLAF-- 447
Query: 444 NNQE 447
NN E
Sbjct: 448 NNYE 451
>SPAC4A8.04 [O] KOG1153 Subtilisin-related protease/Vacuolar protease B
Length = 467
Score = 166 bits (420), Expect = 8e-41
Identities = 120/297 (40%), Positives = 160/297 (53%), Gaps = 19/297 (6%)
Query: 153 SQSRAPRHLARISRRAQLPVIEKDDTSSGLKYHYHEEHQGEGVFVYVLDSGVNHDHPDFE 212
+QS AP LARIS ++ ++ DD KY Y + G+ + YV+D+GV+ H +FE
Sbjct: 181 TQSGAPWGLARISHKS----VKYDDIG---KYVY-DSSAGDNITAYVVDTGVSIHHVEFE 232
Query: 213 GRAMKGIDT-TGEGSGDLVGHGTHVAGLIGSKTYGVAKKVNIVEVKVLDSMGKGTVTNII 271
GRA G +G+ D GHGTHVAG I S+ YGVAKK IV VKVL S G GT+ ++I
Sbjct: 233 GRASWGATIPSGDVDEDNNGHGTHVAGTIASRAYGVAKKAEIVAVKVLRSSGSGTMADVI 292
Query: 272 EGLEYVV--NQCIGKKRKCVINMSLGAPGTHSILDKXXXXXXXXXXXXXXXXXXXXXXXC 329
G+E+ V ++ GKK V NMSLG G +LD C
Sbjct: 293 AGVEWTVRHHKSSGKKTS-VGNMSLGG-GNSFVLDMAVDSAVTNGVIYAVAAGNEYDDAC 350
Query: 330 WYSPAKVRKAITVAAMDDRTDLMAPFSNWGACIDIWAPGVNVKSL-SNKGRGTFVLSGTS 388
+ SPA +KAITV A D MA FSN+G+C+DI+APG+N+ S T +SGTS
Sbjct: 351 YSSPAASKKAITVGA-STINDQMAYFSNYGSCVDIFAPGLNILSTWIGSNTSTNTISGTS 409
Query: 389 MASPIVAGAAAIY--LDQGADPAEVRKILSKTATRGVFKKRAIAVRPRTPNKVLYTG 443
MA+P VAG +A Y L A +EV+ + K V +I V T N + + G
Sbjct: 410 MATPHVAGLSAYYLGLHPAASASEVKDAIIKMGIHDVL--LSIPVGSSTINLLAFNG 464
>YEL060c [O] KOG1153 Subtilisin-related protease/Vacuolar protease B
Length = 635
Score = 159 bits (403), Expect = 7e-39
Identities = 122/319 (38%), Positives = 160/319 (49%), Gaps = 30/319 (9%)
Query: 153 SQSRAPRHLARISRRAQLPVIEKDDTSSGLKYHYHEEHQGEGVFVYVLDSGVNHDHPDFE 212
+Q+ AP LARIS R +L + S KY Y ++ G GV YV+D+GVN +H DFE
Sbjct: 284 TQNSAPWGLARISHRERL------NLGSFNKYLYDDD-AGRGVTSYVIDTGVNINHKDFE 336
Query: 213 GRAMKGIDTTGEGSGDLVG--HGTHVAGLIGSKTYGVAKKVNIVEVKVLDSMGKGTVTNI 270
RA+ G T DL G HGTH AG I SK YGVAK N+V VKVL S G GT++++
Sbjct: 337 KRAIWG-KTIPLNDEDLDGNGHGTHCAGTIASKHYGVAKNANVVAVKVLRSNGSGTMSDV 395
Query: 271 IEGLEYV--VNQCIGKKRK-----CVINMSLGAPGTHSILDKXXXXXXXXXXXXXXXXXX 323
++G+EY +Q +++K NMSLG G LD
Sbjct: 396 VKGVEYAAKAHQKEAQEKKKGFKGSTANMSLGG-GKSPALDLAVNAAVEVGIHFAVAAGN 454
Query: 324 XXXXXCWYSPAKVRKAITVAAMDDRTDLMAPFSNWGACIDIWAPGVNVKS-LSNKGRGTF 382
C SPA KAITV A +D A FSNWG C+D++APG+N+ S T
Sbjct: 455 ENQDACNTSPASADKAITVGA-STLSDDRAYFSNWGKCVDVFAPGLNILSTYIGSDDATA 513
Query: 383 VLSGTSMASPIVAGAAAIYLD-QGADPAEVRKILSKTATRGVFKKRAIAVRPR------- 434
LSGTSMASP VAG +L Q +E ++ + T KK+ I +
Sbjct: 514 TLSGTSMASPHVAGLLTYFLSLQPGSDSEFFELGQDSLTPQQLKKKLIHYSTKDILFDIP 573
Query: 435 --TPNKVLYTGNNQEDDEF 451
TPN ++Y G Q+ F
Sbjct: 574 EDTPNVLIYNGGGQDLSAF 592
>ECU01g1130 [O] KOG1153 Subtilisin-related protease/Vacuolar protease B
Length = 465
Score = 112 bits (280), Expect = 1e-24
Identities = 85/267 (31%), Positives = 124/267 (45%), Gaps = 57/267 (21%)
Query: 190 HQGEGVFVYVLDSGVNHDHPDFEGRAMKGIDTTGEGSG-DLVGHGTHVAGLIGSKTYGVA 248
+ G+GV VYVLD+GV +HP+F GRA G + + D GHGTH AG+IG K +GV
Sbjct: 137 NSGKGVDVYVLDTGVEIEHPEFGGRARWGANFVPKSPDRDEHGHGTHCAGVIGGKNFGVT 196
Query: 249 KKVNIVEVKVLDSMGKGTVTNIIEGL--------------------EYVVN--------- 279
K+ +I+ VKVLD G G + +++G+ EY+ +
Sbjct: 197 KESSIIAVKVLDKYGSGMTSRLLQGVDFVIKEHEKKKDELYNAAADEYLSSGGSSDIEIE 256
Query: 280 ---------------------QCIGKKR---KCVINMSLGAPGTHSILDKXXXXXXXXXX 315
I +K K V+N+S+G ++ L+
Sbjct: 257 MDGSESFSFVQPETPSIQRLVDAISRKALQPKTVVNLSVGG-FRNAALNFAIEYASRLGI 315
Query: 316 XXXXXXXXXXXXXCWYSPAKVRKAITVAAMDDRTDLMAPFSNWGACIDIWAPGVNVKSLS 375
C +SP R AIT A R D +A FSN+G C++++APGV++ S S
Sbjct: 316 HFSTAAGNEHEDACDFSPGSSRAAITTGASTYR-DTVAFFSNFGKCVNVFAPGVDILS-S 373
Query: 376 NKGRGTFVLSGTSMASPIVAGAAAIYL 402
G ++SGTSMA+P +GA A YL
Sbjct: 374 WIGGTQKIVSGTSMAAPHTSGAIAAYL 400
>ECU03g1180 [O] KOG1153 Subtilisin-related protease/Vacuolar protease B
Length = 535
Score = 66.2 bits (160), Expect = 1e-10
Identities = 57/219 (26%), Positives = 96/219 (43%), Gaps = 24/219 (10%)
Query: 186 YHEEHQGEGVFVYVLDSGVNHDHPDFEGRAMKGIDTTGEGSGDLVGHGTHVAGLIGSKTY 245
Y H G+GV +++LD+ ++ +M G HG +A L+ KT
Sbjct: 240 YEYYHTGKGVNIFLLDTAIS---------SMDGACNLSGRLEACNAHGNVMAELLVGKTN 290
Query: 246 GVAKKVNIVEVKVLDSMGKGTVTNIIEGLEYVVNQCIGKKRKCVINMSLGAPGTHSI-LD 304
G AK + + V+D GK ++ +I GLE + ++ + G G +S L+
Sbjct: 291 GFAKDSRLSVLDVVDCDGKVALSEMIHGLEGL------RESGGPSILVFGVSGPYSASLN 344
Query: 305 KXXXXXXXXXXXXXXXXXXXXXXXCWYSPAKVRKAITVAAMDDRTDLMAPFSNWGACIDI 364
C YSP + I V ++D + + FSN G CI +
Sbjct: 345 SAVDRISSRGTVVVSPAGNSHDQSCNYSPGSSKSVINVGSVDKHAGI-SRFSNHGDCIRM 403
Query: 365 WAPGVNVKSLSNKGRGTFVLSGTSMASPIVAGAAAIYLD 403
+APG +V S+ L+GTS+++ IVA + A++L+
Sbjct: 404 FAPGEDVLQDSS-------LTGTSLSAAIVASSIALFLE 435
>Hs4506775 [O] KOG4266 Subtilisin kexin isozyme-1/site 1 protease subtilase
superfamily
Length = 1052
Score = 63.9 bits (154), Expect = 5e-10
Identities = 67/253 (26%), Positives = 103/253 (40%), Gaps = 28/253 (11%)
Query: 190 HQGEGVFVYVLDSGVNHDHPDFEGRAMKGIDTTGEGSGDLVGHGTHVAGLIGS--KTYGV 247
+ G V V V D+G++ HP F+ + T D +GHGT VAG+I S + G
Sbjct: 207 YTGANVRVAVFDTGLSEKHPHFKNVKERTNWTNERTLDDGLGHGTFVAGVIASMRECQGF 266
Query: 248 AKKVNIVEVKVLDSMGKGTVTNIIEGLEYVVNQCIGKKRKCVINMSLGAPG--THSILDK 305
A + +V + + ++ Y + K+ V+N+S+G P H +DK
Sbjct: 267 APDAELHIFRVFTNNQVSYTSWFLDAFNYAI-----LKKIDVLNLSIGGPDFMDHPFVDK 321
Query: 306 XXXXXXXXXXXXXXXXXXXXXXXCWYSPAKVRKAITVAAMDDRTDLMAPFSNWGACI--- 362
+PA I V +D D +A FS+ G
Sbjct: 322 VWELTANNVIMVSAIGNDGPLYGTLNNPADQMDVIGVGGIDFE-DNIARFSSRGMTTWEL 380
Query: 363 ---------DIWAPGVNVKSLSNKGRGTFVLSGTSMASPIVAGAAAIYLD-----QGADP 408
DI G V+ KG G LSGTS+ASP+VAGA + + + +P
Sbjct: 381 PGGYGRMKPDIVTYGAGVRGSGVKG-GCRALSGTSVASPVVAGAVTLLVSTVQKRELVNP 439
Query: 409 AEVRKILSKTATR 421
A +++ L +A R
Sbjct: 440 ASMKQALIASARR 452
>At5g19660 [O] KOG4266 Subtilisin kexin isozyme-1/site 1 protease subtilase
superfamily
Length = 1038
Score = 53.1 bits (126), Expect = 9e-07
Identities = 56/237 (23%), Positives = 92/237 (38%), Gaps = 24/237 (10%)
Query: 181 GLKYHYHEEHQGEGVFVYVLDSGVNHDHPDFEGRAMKGIDTTGEGSGDLVGHGTHVAGLI 240
G + + + + G V + + D+G+ DHP F + T + D +GHGT VAG+I
Sbjct: 192 GADHLWKKGYTGAKVKMAIFDTGIRADHPHFRKIKERTNWTNEDTLNDNLGHGTFVAGVI 251
Query: 241 GSKT---YGVAKKVNIVEVKVLDSMGKGTVTNIIEGLEYVVNQCIGKKRKCVINMSLGAP 297
+ G A I +V + ++ Y + + V+N+S+G P
Sbjct: 252 AGRNPECLGFASDTEIYAFRVFTDAQVSYTSWFLDAFNYAIATDMD-----VLNLSIGGP 306
Query: 298 GTHSI--LDKXXXXXXXXXXXXXXXXXXXXXXXCWYSPAKVRKAITVAAMDDRTDLMAPF 355
+ ++K +PA I V +D+ D +A F
Sbjct: 307 DYLDLPFVEKVWEITASNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGGIDN-DDHIASF 365
Query: 356 SNWGACI------------DIWAPGVNVKSLSNKGRGTFVLSGTSMASPIVAGAAAI 400
S+ G D+ A G ++ S G LSGTS+ASP+VAG +
Sbjct: 366 SSRGMSTWELPHGYGRVKPDVVAYGRDIMG-SKISTGCKSLSGTSVASPVVAGIVCL 421
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.137 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,375,063
Number of Sequences: 60738
Number of extensions: 1037956
Number of successful extensions: 2498
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 2463
Number of HSP's gapped (non-prelim): 11
length of query: 508
length of database: 30,389,216
effective HSP length: 111
effective length of query: 397
effective length of database: 23,647,298
effective search space: 9387977306
effective search space used: 9387977306
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)