ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI1626 good U KOG3324 Intracellular trafficking, secretion, and vesicular transport Mitochondrial import inner membrane translocase, subunit TIM23
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI1626 579571 578915 -219
(219 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YNR017w [U] KOG3324 Mitochondrial import inner membrane transloc... 255 4e-68
SPCC16A11.09c [U] KOG3324 Mitochondrial import inner membrane tr... 140 1e-33
At1g17530 [U] KOG3324 Mitochondrial import inner membrane transl... 59 7e-09
At1g72750 [U] KOG3324 Mitochondrial import inner membrane transl... 55 7e-08
Hs5454122 [U] KOG3324 Mitochondrial import inner membrane transl... 52 8e-07
Hs22053344 [U] KOG3324 Mitochondrial import inner membrane trans... 49 4e-06
>YNR017w [U] KOG3324 Mitochondrial import inner membrane translocase subunit
TIM23
Length = 222
Score = 255 bits (651), Expect = 4e-68
Identities = 130/227 (57%), Positives = 157/227 (68%), Gaps = 15/227 (6%)
Query: 1 MSWLFGGKKSSTDS------QENTTPVEQNDDKLKQTLGFDPXXXXXXXXXXXXPGGL-- 52
MSWLFG K + D+ Q+ T P E + LKQ+LGF+P PGG+
Sbjct: 1 MSWLFGDKTPTDDANAAVGGQDTTKPKELS---LKQSLGFEPNINNIISG----PGGMHV 53
Query: 53 SPSQLHPLAGLERGVEYMDLEEEQLSSMEGSQGLIPSRGWTDDLCXXXXXXXXXXXXXXX 112
++LHPLAGL++GVEY+DLEEEQLSS+EGSQGLIPSRGWTDDLC
Sbjct: 54 DTARLHPLAGLDKGVEYLDLEEEQLSSLEGSQGLIPSRGWTDDLCYGTGAVYLLGLGIGG 113
Query: 113 XXXFIEGLKNIPPNSPGKLQLNTVLNHITRRGPFLGNNAGVLALTYNLINSVIDSFRGKH 172
++GL+NIPPNSPGKLQLNTVLNHIT+RGPFLGNNAG+LAL+YN+INS ID+ RGKH
Sbjct: 114 FSGMMQGLQNIPPNSPGKLQLNTVLNHITKRGPFLGNNAGILALSYNIINSTIDALRGKH 173
Query: 173 DVAGSVAAGALTGAIFRSSRGLKPMGYXXXXXXXXXXGWCGIKSVIL 219
D AGS+ AGALTGA+F+SS+GLKPMGY WC +K +L
Sbjct: 174 DTAGSIGAGALTGALFKSSKGLKPMGYSSAMVAAACAVWCSVKKRLL 220
>SPCC16A11.09c [U] KOG3324 Mitochondrial import inner membrane translocase subunit
TIM23
Length = 210
Score = 140 bits (354), Expect = 1e-33
Identities = 83/216 (38%), Positives = 109/216 (50%), Gaps = 23/216 (10%)
Query: 1 MSWLFGGKK-----SSTDSQENTTPVEQNDDKLKQTLGFDPXXXXXXXXXXXXPGGLSPS 55
MSWLF K S DS E P E + FDP +
Sbjct: 1 MSWLFTRNKEEEPTSKIDSSELQVPTEATASDILSGSEFDP------------------A 42
Query: 56 QLHPLAGLERGVEYMDLEEEQLSSMEGSQGLIPSRGWTDDLCXXXXXXXXXXXXXXXXXX 115
+LHPLA L++ ++Y+ +EE+ LS++ G IPSRGW DDLC
Sbjct: 43 KLHPLADLDKPLDYLLIEEDALSTLPGDSMAIPSRGWQDDLCYGTGTSYLSGLAIGGLWG 102
Query: 116 FIEGLKNIPPNSPGKLQLNTVLNHITRRGPFLGNNAGVLALTYNLINSVIDSFRGKHDVA 175
EG+K + +L+LN +LN +TRRGPF+GN+ GVLAL YN INS+I R KH
Sbjct: 103 LNEGMKKTKDITSTRLRLNGILNGVTRRGPFVGNSLGVLALVYNGINSLIGYKRQKHGWE 162
Query: 176 GSVAAGALTGAIFRSSRGLKPMGYXXXXXXXXXXGW 211
SVAAGALTGA+++S+RGL+ M W
Sbjct: 163 NSVAAGALTGALYKSTRGLRAMAISSSLVATAAGIW 198
>At1g17530 [U] KOG3324 Mitochondrial import inner membrane translocase subunit
TIM23
Length = 194
Score = 58.5 bits (140), Expect = 7e-09
Identities = 33/112 (29%), Positives = 51/112 (45%)
Query: 84 QGLIPSRGWTDDLCXXXXXXXXXXXXXXXXXXFIEGLKNIPPNSPGKLQLNTVLNHITRR 143
+ L R W ++L G+K+ KL++N +LN +
Sbjct: 47 ESLKQRRSWGENLTFYTGTGYLAGSVAGASAGIFSGIKSFENGDTTKLKINRILNSSGQA 106
Query: 144 GPFLGNNAGVLALTYNLINSVIDSFRGKHDVAGSVAAGALTGAIFRSSRGLK 195
G GN G++ L Y I S + + K DV SV AG TGA+FR++RG++
Sbjct: 107 GRTWGNRVGIVGLIYAGIESGVVAVTDKDDVWTSVVAGLGTGAVFRAARGVR 158
>At1g72750 [U] KOG3324 Mitochondrial import inner membrane translocase subunit
TIM23
Length = 188
Score = 55.1 bits (131), Expect = 7e-08
Identities = 33/112 (29%), Positives = 51/112 (45%)
Query: 84 QGLIPSRGWTDDLCXXXXXXXXXXXXXXXXXXFIEGLKNIPPNSPGKLQLNTVLNHITRR 143
+ L R W ++L I G+K+ KL++N +LN +
Sbjct: 48 EALRQRRSWGENLTFYTGTAYLGGSVAGASVGVITGVKSFESGDTTKLKINRILNSSGQT 107
Query: 144 GPFLGNNAGVLALTYNLINSVIDSFRGKHDVAGSVAAGALTGAIFRSSRGLK 195
G GN G++ L Y I S I + + DV SV AG TGA+ R++RG++
Sbjct: 108 GRTWGNRIGIIGLVYAGIESGIVAATDRDDVWTSVVAGLGTGAVCRAARGVR 159
>Hs5454122 [U] KOG3324 Mitochondrial import inner membrane translocase subunit
TIM23
Length = 209
Score = 51.6 bits (122), Expect = 8e-07
Identities = 27/77 (35%), Positives = 42/77 (54%)
Query: 119 GLKNIPPNSPGKLQLNTVLNHITRRGPFLGNNAGVLALTYNLINSVIDSFRGKHDVAGSV 178
GLK + K + +LN +TR+G N G LAL Y+ +I+ RG D +V
Sbjct: 99 GLKETQNMAWSKPRNVQILNMVTRQGALWANTLGSLALLYSAFGVIIEKTRGAEDDLNTV 158
Query: 179 AAGALTGAIFRSSRGLK 195
AAG +TG +++ + GL+
Sbjct: 159 AAGTMTGMLYKCTGGLR 175
>Hs22053344 [U] KOG3324 Mitochondrial import inner membrane translocase subunit
TIM23
Length = 178
Score = 49.3 bits (116), Expect = 4e-06
Identities = 26/75 (34%), Positives = 40/75 (52%)
Query: 119 GLKNIPPNSPGKLQLNTVLNHITRRGPFLGNNAGVLALTYNLINSVIDSFRGKHDVAGSV 178
GLK + K + +LN +TR+G N G LAL Y+ +I+ RG D +V
Sbjct: 99 GLKETQNMAWSKPRNVQILNMVTRQGALWANTLGSLALLYSAFGVIIEKTRGAEDDLNTV 158
Query: 179 AAGALTGAIFRSSRG 193
AAG +TG +++ + G
Sbjct: 159 AAGTMTGMLYKCTGG 173
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.137 0.410
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,398,315
Number of Sequences: 60738
Number of extensions: 402782
Number of successful extensions: 613
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 605
Number of HSP's gapped (non-prelim): 6
length of query: 219
length of database: 30,389,216
effective HSP length: 102
effective length of query: 117
effective length of database: 24,193,940
effective search space: 2830690980
effective search space used: 2830690980
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)