ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI1692 good G KOG2944 Carbohydrate transport and metabolism Glyoxalase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI1692 606977 607990 338
(338 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YML004c [G] KOG2944 Glyoxalase 431 e-121
SPBC12C2.12c [G] KOG2944 Glyoxalase 283 2e-76
7304232 [G] KOG2944 Glyoxalase 145 1e-34
At1g08110 [G] KOG2944 Glyoxalase 141 1e-33
Hs5729842 [G] KOG2944 Glyoxalase 134 2e-31
At1g11840 [G] KOG2943 Predicted glyoxalase 101 1e-21
At1g67280 [G] KOG2943 Predicted glyoxalase 99 9e-21
>YML004c [G] KOG2944 Glyoxalase
Length = 326
Score = 431 bits (1108), Expect = e-121
Identities = 214/329 (65%), Positives = 248/329 (75%), Gaps = 13/329 (3%)
Query: 16 MSTETTAKYYPKIVESAQADQSLKLNHTCFRVKDPKVTVAFYQEQFGMKLLDHKKFPDMK 75
MST++T YP +E A D +L LNHTC RVKDP TV FY E FGMKLL K F + K
Sbjct: 1 MSTDSTR--YPIQIEKASNDPTLLLNHTCLRVKDPARTVKFYTEHFGMKLLSRKDFEEAK 58
Query: 76 FDLYFLSFPNKQFSNNSQGAIDVFRENGILELTHNYGTESDPAYKVNNGNEEPHRGFGHI 135
F LYFLSFP N G DVF +G+LELTHN+GTE +P YK+NNGNEEPHRGFGHI
Sbjct: 59 FSLYFLSFPKDDIPKNKNGEPDVFSAHGVLELTHNWGTEKNPDYKINNGNEEPHRGFGHI 118
Query: 136 CFSVSNLEAECERLESNGVKFKKRLTDGSQRNIAFALDPNGYWIELIQNNESGE------ 189
CFSVS++ CE LES GVKFKKRL++G Q++IAFAL P+GYWIELI + G+
Sbjct: 119 CFSVSDINKTCEELESQGVKFKKRLSEGRQKDIAFALGPDGYWIELITYSREGQEYPKGS 178
Query: 190 -GNNYKFNHTMVRVKDPIKSLEFYQNVLGMKILDVSDHSNAKFTLYFLGYENDQKGIARG 248
GN KFNHTM+R+K+P +SLEFYQNVLGMK+L S+H +AKFTLYFLGY K +
Sbjct: 179 VGN--KFNHTMIRIKNPTRSLEFYQNVLGMKLLRTSEHESAKFTLYFLGY-GVPKTDSVF 235
Query: 249 SRESILELTHNWGTENDPDFAYHTGNTEPQGYGHICISNKDPATLCAEIEKLYPD-IQWS 307
S ES+LELTHNWGTENDP+F YH GN+EPQGYGHICIS D LC EIE Y D IQWS
Sbjct: 236 SCESVLELTHNWGTENDPNFHYHNGNSEPQGYGHICISCDDAGALCKEIEVKYGDKIQWS 295
Query: 308 PKFNQGKMKNLAFIKDPDGYSIEVVPYGL 336
PKFNQG+MKN+AF+KDPDGYSIEVVP+GL
Sbjct: 296 PKFNQGRMKNIAFLKDPDGYSIEVVPHGL 324
>SPBC12C2.12c [G] KOG2944 Glyoxalase
Length = 302
Score = 283 bits (725), Expect = 2e-76
Identities = 145/298 (48%), Positives = 194/298 (64%), Gaps = 13/298 (4%)
Query: 39 KLNHTCFRVKDPKVTVAFYQEQFGMKLLDHKKFPDMKFDLYFLSFPNKQFSNNSQGAIDV 98
KLNHT RVKD ++ FY E FGMKL+D F + +F L FL+F N+ ++
Sbjct: 11 KLNHTMIRVKDLDKSLKFYTEVFGMKLIDQWVFEENEFSLSFLAFDGPGALNHG---VER 67
Query: 99 FRENGILELTHNYGTESDPAYKVNNGNEEPHRGFGHICFSVSNLEAECERLESNGVKFKK 158
+ GILELT+N+GTE NGN EP RGFGHICF+V N+E+ C LES GV FKK
Sbjct: 68 SKREGILELTYNFGTEKKEGPVYINGNTEPKRGFGHICFTVDNIESACAYLESKGVSFKK 127
Query: 159 RLTDGSQRNIAFALDPNGYWIELIQNNES---GEGNNYKFNHTMVRVKDPIKSLEFYQNV 215
+L+DG ++IAFALDP+ YWIEL+ +E+ +N++FNHTMVRVKDP S+ FY+
Sbjct: 128 KLSDGKMKHIAFALDPDNYWIELVSQSETKPKANISNFRFNHTMVRVKDPEPSIAFYEK- 186
Query: 216 LGMKILDVSDHSNAKFTLYFLGYENDQKGIARGSRESILELTHNWGTENDPDFAYHTGNT 275
LGMK++D +DH N KFT YFL Y +D + R RE +LELTHNWGTE + YH GN
Sbjct: 187 LGMKVIDKADHPNGKFTNYFLAYPSD---LPRHDREGLLELTHNWGTEKESGPVYHNGND 243
Query: 276 -EPQGYGHICISNKDPATLCAEIEKLYPDIQWSPKFNQGKMKNLAFIKDPDGYSIEVV 332
+ +GYGH+CIS + C++ E + + K G+MK++AF+ DPD Y +EV+
Sbjct: 244 GDEKGYGHVCISVDNINAACSKFEA--EGLPFKKKLTDGRMKDIAFLLDPDNYWVEVI 299
Score = 140 bits (353), Expect = 3e-33
Identities = 64/147 (43%), Positives = 98/147 (66%), Gaps = 10/147 (6%)
Query: 37 SLKLNHTCFRVKDPKVTVAFYQEQFGMKLLDHKKFPDMKFDLYFLSFPNKQFSNNSQGAI 96
+ + NHT RVKDP+ ++AFY E+ GMK++D P+ KF YFL++P+ ++ +G
Sbjct: 164 NFRFNHTMVRVKDPEPSIAFY-EKLGMKVIDKADHPNGKFTNYFLAYPSDLPRHDREG-- 220
Query: 97 DVFRENGILELTHNYGTESDPAYKVNNGNEEPHRGFGHICFSVSNLEAECERLESNGVKF 156
+LELTHN+GTE + +NGN+ +G+GH+C SV N+ A C + E+ G+ F
Sbjct: 221 -------LLELTHNWGTEKESGPVYHNGNDGDEKGYGHVCISVDNINAACSKFEAEGLPF 273
Query: 157 KKRLTDGSQRNIAFALDPNGYWIELIQ 183
KK+LTDG ++IAF LDP+ YW+E+I+
Sbjct: 274 KKKLTDGRMKDIAFLLDPDNYWVEVIE 300
Score = 121 bits (303), Expect = 2e-27
Identities = 65/146 (44%), Positives = 89/146 (60%), Gaps = 6/146 (4%)
Query: 191 NNYKFNHTMVRVKDPIKSLEFYQNVLGMKILDVSDHSNAKFTLYFLGYEND---QKGIAR 247
+ YK NHTM+RVKD KSL+FY V GMK++D +F+L FL ++ G+ R
Sbjct: 8 STYKLNHTMIRVKDLDKSLKFYTEVFGMKLIDQWVFEENEFSLSFLAFDGPGALNHGVER 67
Query: 248 GSRESILELTHNWGTENDPDFAYHTGNTEP-QGYGHICISNKDPATLCAEIEKLYPDIQW 306
RE ILELT+N+GTE Y GNTEP +G+GHIC + + + CA +E + +
Sbjct: 68 SKREGILELTYNFGTEKKEGPVYINGNTEPKRGFGHICFTVDNIESACAYLES--KGVSF 125
Query: 307 SPKFNQGKMKNLAFIKDPDGYSIEVV 332
K + GKMK++AF DPD Y IE+V
Sbjct: 126 KKKLSDGKMKHIAFALDPDNYWIELV 151
>7304232 [G] KOG2944 Glyoxalase
Length = 176
Score = 145 bits (365), Expect = 1e-34
Identities = 80/160 (50%), Positives = 98/160 (61%), Gaps = 11/160 (6%)
Query: 180 ELIQNNESGEGNNYKFNHTMVRVKDPIKSLEFYQNVLGMKILDVSDHSNAKFTLYFLGYE 239
EL Q +S ++ F TM R+KDP KSL FY VLGM +L D AKF+LYFLGYE
Sbjct: 14 ELCQKPDSST-KDFLFQQTMYRIKDPRKSLPFYTGVLGMTLLVKLDFPEAKFSLYFLGYE 72
Query: 240 N--------DQKGIARGSRESILELTHNWGTENDPDFAYHTGNTEPQGYGHICISNKDPA 291
N Q+ SR++ +ELTHNWGTE DPD YHTGNT+P+G+GHI I D
Sbjct: 73 NATDVPKDPKQRRSWALSRKATIELTHNWGTERDPDQNYHTGNTDPRGFGHIGIMVPDVY 132
Query: 292 TLCAEIEKLYPDIQWSPKFNQGKMKNLAFIKDPDGYSIEV 331
C ++L D P + G+MK LAFIKDPDGY IE+
Sbjct: 133 AACQRFQELGVDFVKKP--DDGRMKGLAFIKDPDGYWIEI 170
Score = 114 bits (286), Expect = 2e-25
Identities = 59/141 (41%), Positives = 80/141 (55%), Gaps = 3/141 (2%)
Query: 43 TCFRVKDPKVTVAFYQEQFGMKLLDHKKFPDMKFDLYFLSFPNKQF--SNNSQGAIDVFR 100
T +R+KDP+ ++ FY GM LL FP+ KF LYFL + N + Q
Sbjct: 31 TMYRIKDPRKSLPFYTGVLGMTLLVKLDFPEAKFSLYFLGYENATDVPKDPKQRRSWALS 90
Query: 101 ENGILELTHNYGTESDPAYKVNNGNEEPHRGFGHICFSVSNLEAECERLESNGVKFKKRL 160
+ELTHN+GTE DP + GN +P RGFGHI V ++ A C+R + GV F K+
Sbjct: 91 RKATIELTHNWGTERDPDQNYHTGNTDP-RGFGHIGIMVPDVYAACQRFQELGVDFVKKP 149
Query: 161 TDGSQRNIAFALDPNGYWIEL 181
DG + +AF DP+GYWIE+
Sbjct: 150 DDGRMKGLAFIKDPDGYWIEI 170
>At1g08110 [G] KOG2944 Glyoxalase
Length = 185
Score = 141 bits (356), Expect = 1e-33
Identities = 72/159 (45%), Positives = 98/159 (61%), Gaps = 11/159 (6%)
Query: 182 IQNNESGEGNNYKFNHTMVRVKDPIKSLEFYQNVLGMKILDVSDHSNAKFTLYFLGYEN- 240
+ N Y TM R+KDP SL+FY VLGM +L D S KF+LYFLGYE+
Sbjct: 15 LSTNRDEATKGYIMQQTMFRIKDPKASLDFYSRVLGMSLLKRLDFSEMKFSLYFLGYEDT 74
Query: 241 -------DQKGIARGSRESILELTHNWGTENDPDF-AYHTGNTEPQGYGHICISNKDPAT 292
++ + + + +ELTHNWGTE+DP+F YH GN+EP+G+GHI ++ D
Sbjct: 75 TTAPTDPTERTVWTFGQPATIELTHNWGTESDPEFKGYHNGNSEPRGFGHIGVTVDDVHK 134
Query: 293 LCAEIEKLYPDIQWSPKFNQGKMKNLAFIKDPDGYSIEV 331
C E+L ++++ K N GKMKN+AFIKDPDGY IE+
Sbjct: 135 ACERFEEL--GVEFAKKPNDGKMKNIAFIKDPDGYWIEI 171
Score = 135 bits (339), Expect = 1e-31
Identities = 68/152 (44%), Positives = 95/152 (61%), Gaps = 4/152 (2%)
Query: 33 QADQSLKLNHTCFRVKDPKVTVAFYQEQFGMKLLDHKKFPDMKFDLYFLSFPNKQFS--N 90
+A + + T FR+KDPK ++ FY GM LL F +MKF LYFL + + + +
Sbjct: 21 EATKGYIMQQTMFRIKDPKASLDFYSRVLGMSLLKRLDFSEMKFSLYFLGYEDTTTAPTD 80
Query: 91 NSQGAIDVFRENGILELTHNYGTESDPAYK-VNNGNEEPHRGFGHICFSVSNLEAECERL 149
++ + F + +ELTHN+GTESDP +K +NGN EP RGFGHI +V ++ CER
Sbjct: 81 PTERTVWTFGQPATIELTHNWGTESDPEFKGYHNGNSEP-RGFGHIGVTVDDVHKACERF 139
Query: 150 ESNGVKFKKRLTDGSQRNIAFALDPNGYWIEL 181
E GV+F K+ DG +NIAF DP+GYWIE+
Sbjct: 140 EELGVEFAKKPNDGKMKNIAFIKDPDGYWIEI 171
>Hs5729842 [G] KOG2944 Glyoxalase
Length = 184
Score = 134 bits (337), Expect = 2e-31
Identities = 70/149 (46%), Positives = 97/149 (64%), Gaps = 10/149 (6%)
Query: 192 NYKFNHTMVRVKDPIKSLEFYQNVLGMKILDVSDHSNAKFTLYFLGYEN--------DQK 243
++ TM+RVKDP KSL+FY VLGM ++ D KF+LYFL YE+ D+K
Sbjct: 29 DFLLQQTMLRVKDPKKSLDFYTRVLGMTLIQKCDFPIMKFSLYFLAYEDKNDIPKEKDEK 88
Query: 244 GIARGSRESILELTHNWGTENDPDFAYHTGNTEPQGYGHICISNKDPATLCAEIEKLYPD 303
SR++ LELTHNWGTE+D +YH GN++P+G+GHI I+ D + C E+L
Sbjct: 89 IAWALSRKATLELTHNWGTEDDATQSYHNGNSDPRGFGHIGIAVPDVYSACKRFEEL--G 146
Query: 304 IQWSPKFNQGKMKNLAFIKDPDGYSIEVV 332
+++ K + GKMK LAFI+DPDGY IE++
Sbjct: 147 VKFVKKPDDGKMKGLAFIQDPDGYWIEIL 175
Score = 127 bits (318), Expect = 3e-29
Identities = 68/160 (42%), Positives = 91/160 (56%), Gaps = 6/160 (3%)
Query: 32 AQADQSLK---LNHTCFRVKDPKVTVAFYQEQFGMKLLDHKKFPDMKFDLYFLSFPNKQF 88
+ AD S K L T RVKDPK ++ FY GM L+ FP MKF LYFL++ +K
Sbjct: 21 SDADPSTKDFLLQQTMLRVKDPKKSLDFYTRVLGMTLIQKCDFPIMKFSLYFLAYEDKND 80
Query: 89 --SNNSQGAIDVFRENGILELTHNYGTESDPAYKVNNGNEEPHRGFGHICFSVSNLEAEC 146
+ LELTHN+GTE D +NGN +P RGFGHI +V ++ + C
Sbjct: 81 IPKEKDEKIAWALSRKATLELTHNWGTEDDATQSYHNGNSDP-RGFGHIGIAVPDVYSAC 139
Query: 147 ERLESNGVKFKKRLTDGSQRNIAFALDPNGYWIELIQNNE 186
+R E GVKF K+ DG + +AF DP+GYWIE++ N+
Sbjct: 140 KRFEELGVKFVKKPDDGKMKGLAFIQDPDGYWIEILNPNK 179
>At1g11840 [G] KOG2943 Predicted glyoxalase
Length = 283
Score = 101 bits (252), Expect = 1e-21
Identities = 76/250 (30%), Positives = 111/250 (44%), Gaps = 45/250 (18%)
Query: 42 HTCFRVKDPKVTVAFYQEQFGMKLLDHKKFPDMKFDLYFLSFPNKQFSNNSQGAIDVFRE 101
H +RV D T+ FY E FGMKLL + P+ K+ FL F +
Sbjct: 20 HVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPE-------------TS 66
Query: 102 NGILELTHNYGTESDPAYKVNNGNEEPHRGFGHICFSVSNLEAECERLESNG--VKFKKR 159
N ++ELT+NYG S Y + GFGH S ++ E + + G V +
Sbjct: 67 NFVVELTYNYGVSS---YDIGT-------GFGHFAISTQDVSKLVENVRAKGGNVTREPG 116
Query: 160 LTDGSQRNIAFALDPNGYWIELIQNNESGEGNNYKFNHTMVRVKDPIKSLEFYQNVLGMK 219
G IAF DP+GY ELIQ + E F M+RV D ++++FY+ LGM+
Sbjct: 117 PVKGGGSVIAFVKDPDGYTFELIQRGPTPE----PFCQVMLRVGDLDRAIKFYEKALGMR 172
Query: 220 ILDVSDHSNAKFTLYFLGYENDQKGIARGSRESILELTHNWGTENDPDFAYHTGNTEPQG 279
+L + K+T+ +GY + + I +LELT+N+ T T+
Sbjct: 173 LLRKIERPEYKYTIGMMGYAEEYESI-------VLELTYNYDV---------TEYTKGNA 216
Query: 280 YGHICISNKD 289
Y I I D
Sbjct: 217 YAQIAIGTDD 226
Score = 74.3 bits (181), Expect = 2e-13
Identities = 45/145 (31%), Positives = 73/145 (50%), Gaps = 16/145 (11%)
Query: 191 NNYKFNHTMVRVKDPIKSLEFYQNVLGMKILDVSDHSNAKFTLYFLGYENDQKGIARGSR 250
+N +F H + RV D +++EFY V GMK+L D K++ FLG+ +
Sbjct: 14 DNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPETSNF----- 68
Query: 251 ESILELTHNWGTENDPDFAYHTGNTEPQGYGHICISNKDPATLCAEIEKLYPDIQWSPKF 310
++ELT+N+G + Y G G+GH IS +D + L + ++ P
Sbjct: 69 --VVELTYNYGVSS-----YDIGT----GFGHFAISTQDVSKLVENVRAKGGNVTREPGP 117
Query: 311 NQGKMKNLAFIKDPDGYSIEVVPYG 335
+G +AF+KDPDGY+ E++ G
Sbjct: 118 VKGGGSVIAFVKDPDGYTFELIQRG 142
>At1g67280 [G] KOG2943 Predicted glyoxalase
Length = 350
Score = 99.0 bits (245), Expect = 9e-21
Identities = 79/291 (27%), Positives = 125/291 (42%), Gaps = 45/291 (15%)
Query: 39 KLNHTCFRVKDPKVTVAFYQEQFGMKLLDHKKFPDMKFDLYFLSFPNKQFSNNSQGAIDV 98
++ H +RV D T+ FY E GMKLL + P+ K+ FL + +
Sbjct: 88 RMLHVVYRVGDMDRTIKFYTECLGMKLLRKRDIPEEKYTNAFLGYGPED----------- 136
Query: 99 FRENGILELTHNYGTESDPAYKVNNGNEEPHRGFGHICFSVSNLEAECERLESNGVKFKK 158
+ ++ELT+NYG + Y + GFGH +V ++ E +++ G K +
Sbjct: 137 --SHFVIELTYNYGVDK---YDIG-------AGFGHFGIAVDDVAKTVELVKAKGGKVSR 184
Query: 159 RL--TDGSQRNIAFALDPNGYWIELIQNNESGEGNNYKFNHTMVRVKDPIKSLEFYQNVL 216
G + IAF DP+GY EL++ + E M+RV D ++++FY+
Sbjct: 185 EPGPVKGGKTVIAFIEDPDGYKFELLERGPTPE----PLCQVMLRVGDLDRAIKFYEKAF 240
Query: 217 GMKILDVSDHSNAKFTLYFLGYENDQKGIARGSRESILELTHNWGTENDPDFAYHTGNTE 276
GM++L D+ K+T+ +GY + K +LELT+N+G Y GN
Sbjct: 241 GMELLRTRDNPEYKYTIAMMGYGPEDK-------FPVLELTYNYGVTE-----YDKGN-- 286
Query: 277 PQGYGHICISNKDPATLCAEIEKLYPDIQWSPKFNQGKMKNLAFIKDPDGY 327
Y I I D I+ I P G + DPDG+
Sbjct: 287 --AYAQIAIGTDDVYKTAEAIKLFGGKITREPGPLPGISTKITACLDPDGW 335
Score = 63.9 bits (154), Expect = 3e-10
Identities = 43/139 (30%), Positives = 66/139 (46%), Gaps = 16/139 (11%)
Query: 197 HTMVRVKDPIKSLEFYQNVLGMKILDVSDHSNAKFTLYFLGYENDQKGIARGSRESILEL 256
H + RV D ++++FY LGMK+L D K+T FLGY + ++EL
Sbjct: 91 HVVYRVGDMDRTIKFYTECLGMKLLRKRDIPEEKYTNAFLGYGPEDS-------HFVIEL 143
Query: 257 THNWGTENDPDFAYHTGNTEPQGYGHICISNKDPATLCAEIEKLYPDIQWSPKFNQGKMK 316
T+N+G + Y G G+GH I+ D A ++ + P +G
Sbjct: 144 TYNYGVDK-----YDIG----AGFGHFGIAVDDVAKTVELVKAKGGKVSREPGPVKGGKT 194
Query: 317 NLAFIKDPDGYSIEVVPYG 335
+AFI+DPDGY E++ G
Sbjct: 195 VIAFIEDPDGYKFELLERG 213
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.137 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,666,394
Number of Sequences: 60738
Number of extensions: 1041619
Number of successful extensions: 2183
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 2124
Number of HSP's gapped (non-prelim): 14
length of query: 338
length of database: 30,389,216
effective HSP length: 107
effective length of query: 231
effective length of database: 23,890,250
effective search space: 5518647750
effective search space used: 5518647750
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)