ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI1722 good T KOG4143 Signal transduction mechanisms Sigma receptor and C-8 sterol isomerase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactVI1722 616447 615779 -223 
         (223 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YMR202w [T] KOG4143 Sigma receptor and C-8 sterol isomerase           350   9e-97
SPAC20G8.07c [T] KOG4143 Sigma receptor and C-8 sterol isomerase      197   1e-50
Hs5032117 [T] KOG4143 Sigma receptor and C-8 sterol isomerase          97   1e-20

>YMR202w [T] KOG4143 Sigma receptor and C-8 sterol isomerase
          Length = 222

 Score =  350 bits (898), Expect = 9e-97
 Identities = 168/223 (75%), Positives = 192/223 (85%), Gaps = 1/223 (0%)

Query: 1   MKLLKIALLLGTAYYLLNSLLYTWLPTNYIFDAKTLNEISNSVISKYDAESETFDVKVML 60
           MK   + LL+G   Y++N L  TWLPTNY+FD KTLNEI NSVISK++A +E    + +L
Sbjct: 1   MKFFPLLLLIGVVGYIMNVLFTTWLPTNYMFDPKTLNEICNSVISKHNA-AEGLSTEDLL 59

Query: 61  KDLRDALAKHYGDDYINQWVDEEWVFNNAGGAMGQMIILHASISEYLIIFGSAVGTEGHT 120
           +D+RDALA HYGD+YIN++V EEWVFNNAGGAMGQMIILHAS+SEYLI+FG+AVGTEGHT
Sbjct: 60  QDVRDALASHYGDEYINRYVKEEWVFNNAGGAMGQMIILHASVSEYLILFGTAVGTEGHT 119

Query: 121 GVHFADDHFTILYGEQRASLPYNMEPEIYKPGQTHHLKKGYAKQYSMPSGSFALELAQGW 180
           GVHFADD+FTIL+G Q A+LPY  E E+Y PG THHLKKGYAKQYSMP GSFALELAQGW
Sbjct: 120 GVHFADDYFTILHGTQIAALPYATEAEVYTPGMTHHLKKGYAKQYSMPGGSFALELAQGW 179

Query: 181 IPAMLPFGFLDTFSSTLDIYTLYRTVYLTARDMIKNLIQNHKF 223
           IP MLPFGFLDTFSSTLD+YTLYRTVYLTARDM KNL+QN KF
Sbjct: 180 IPCMLPFGFLDTFSSTLDLYTLYRTVYLTARDMGKNLLQNKKF 222


>SPAC20G8.07c [T] KOG4143 Sigma receptor and C-8 sterol isomerase
          Length = 219

 Score =  197 bits (500), Expect = 1e-50
 Identities = 113/225 (50%), Positives = 138/225 (61%), Gaps = 17/225 (7%)

Query: 1   MKLLK-----IALLLGTAYYLLNSLLYTWLPTNYIFDAKTLNEISNSVISKYDAESETFD 55
           MKL K     I  + G  YY+    L ++    Y FD   L E+S   I+ Y       D
Sbjct: 1   MKLTKFLTVFIPFIAGLIYYIQKYHLRSF----YQFDPAKLQELSKQSIALY-----AND 51

Query: 56  VKVMLKDLRDALAKHYGDDYINQWVDEEWVFNNAGGAMGQMIILHASISEYLIIFGSAVG 115
            K +L DL D L   YGD  I     +EWV NNAGGAMG M ILHAS SEYLI FG+ +G
Sbjct: 52  TKALLYDLSDRLVAEYGD-LITPVNQDEWVHNNAGGAMGTMFILHASFSEYLIFFGTPIG 110

Query: 116 TEGHTGVHFADDHFTILYGEQRASLPYNMEPEIYKPGQTHHLKKGYAKQYSMPSGS--FA 173
           TEGH+GVH ADD+FTIL G Q A+   ++E  +Y PG  H    G+  QYSMPSG   FA
Sbjct: 111 TEGHSGVHMADDYFTILRGRQLAASANDLEARVYLPGDQHVHPWGHTAQYSMPSGEPCFA 170

Query: 174 LELAQGWIPAMLPFGFLDTFSSTLDIYTLYRTVYLTARDMIKNLI 218
           LELAQGWI +MLPFGF+D   ST+D  TLY+TVY TA  M+K+++
Sbjct: 171 LELAQGWIVSMLPFGFMDGLFSTIDFGTLYKTVYFTAGRMLKSVL 215


>Hs5032117 [T] KOG4143 Sigma receptor and C-8 sterol isomerase
          Length = 223

 Score = 97.4 bits (241), Expect = 1e-20
 Identities = 65/208 (31%), Positives = 108/208 (51%), Gaps = 11/208 (5%)

Query: 7   ALLLGTAYYLLNSLLYTWLPT-NYIFDAKTLNEISNSVISKYDAESETFDVKVMLKDLRD 65
           ALLL  A  +L  +++ WL T +++F  + + +++     +Y           ++ +LR 
Sbjct: 13  ALLLAVAA-VLTQVVWLWLGTQSFVFQREEIAQLAR----QYAGLDHELAFSRLIVELRR 67

Query: 66  ALAKHYGDDYINQWVDEEWVFNNAGGAMGQMIILHASISEYLIIFGSAVGTEGHTGVHFA 125
               H   D      + +WVF NAGG MG M +LHAS+SEY+++FG+A+G+ GH+G ++A
Sbjct: 68  LHPGHVLPDE-----ELQWVFVNAGGWMGAMCLLHASLSEYVLLFGTALGSRGHSGRYWA 122

Query: 126 DDHFTILYGEQRASLPYNMEPEIYKPGQTHHLKKGYAKQYSMPSGSFALELAQGWIPAML 185
           +   TI+ G          + E++ PG+T     G A        ++ +E  +G IP+ L
Sbjct: 123 EISDTIISGTFHQWREGTTKSEVFYPGETVVHGPGEATAVEWGPNTWMVEYGRGVIPSTL 182

Query: 186 PFGFLDTFSSTLDIYTLYRTVYLTARDM 213
            F   DT  ST D  TL+ T+   AR +
Sbjct: 183 AFALADTVFSTQDFLTLFYTLRSYARGL 210


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.321    0.138    0.415 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,426,621
Number of Sequences: 60738
Number of extensions: 563512
Number of successful extensions: 1325
Number of sequences better than 1.0e-05: 3
Number of HSP's better than  0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1319
Number of HSP's gapped (non-prelim): 3
length of query: 223
length of database: 30,389,216
effective HSP length: 102
effective length of query: 121
effective length of database: 24,193,940
effective search space: 2927466740
effective search space used: 2927466740
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)