ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI1828 good R KOG3197 General function prediction only Predicted hydrolases of HD superfamily
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI1828 654159 653401 -253
(253 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YBR242w [R] KOG3197 Predicted hydrolases of HD superfamily 298 5e-81
YGL101w [R] KOG3197 Predicted hydrolases of HD superfamily 293 2e-79
SPCC4G3.17 [R] KOG3197 Predicted hydrolases of HD superfamily 180 1e-45
At2g23820 [R] KOG3197 Predicted hydrolases of HD superfamily 131 8e-31
At1g26160 [R] KOG3197 Predicted hydrolases of HD superfamily 129 5e-30
7297141 [R] KOG3197 Predicted hydrolases of HD superfamily 121 8e-28
Hs7705624 [R] KOG3197 Predicted hydrolases of HD superfamily 74 2e-13
CE10532 [R] KOG3197 Predicted hydrolases of HD superfamily 74 2e-13
>YBR242w [R] KOG3197 Predicted hydrolases of HD superfamily
Length = 238
Score = 298 bits (763), Expect = 5e-81
Identities = 138/213 (64%), Positives = 172/213 (79%)
Query: 41 SKMTMEWNPRDHVPAEVKELLSEPQPNYVLAFLHIVEQLKLQKRTGWIDHDIIPCESIAD 100
+++T EW P VP VK LS+P PNY+LAFL++V+QLK+Q+RTG++D I CESI+D
Sbjct: 20 TRLTTEWKPESQVPQYVKNELSKPHPNYILAFLNVVQQLKIQRRTGYLDLGIKECESISD 79
Query: 101 HMYRMGVTTMLIKDPNVDVKKCVRIALVHDIAESLVGDITPFDPVTKEEKHQRELDTITY 160
HMYR+ + TMLIKD V+ KCVRIALVHDIAESLVGDITP DP+ KEEKH+RE +TI Y
Sbjct: 80 HMYRLSIITMLIKDSRVNRDKCVRIALVHDIAESLVGDITPVDPIGKEEKHRREWETIKY 139
Query: 161 LCEEIIKPYNSVAADEILSDWLAYENISSLEARYVKDIDKFEMLVQCFEYEKRHSGTKDL 220
LC +IKPYN +AA EI+ DWLAYEN++SLEARYVKDIDK+EMLVQCFEYE+ + GTK+
Sbjct: 140 LCNALIKPYNEIAAKEIMDDWLAYENVTSLEARYVKDIDKYEMLVQCFEYEREYKGTKNF 199
Query: 221 SQFYGAVSSIKTEEVKSWVSHLVKRRTEFFQQL 253
F+GAV+SIKT+EVK W S LV +R ++F L
Sbjct: 200 DDFFGAVASIKTDEVKGWTSDLVVQRQKYFADL 232
>YGL101w [R] KOG3197 Predicted hydrolases of HD superfamily
Length = 215
Score = 293 bits (749), Expect = 2e-79
Identities = 134/207 (64%), Positives = 165/207 (78%)
Query: 47 WNPRDHVPAEVKELLSEPQPNYVLAFLHIVEQLKLQKRTGWIDHDIIPCESIADHMYRMG 106
W P D++P E+ +LS+P PNY LAFL+I++ LK Q+RTGW+DH I PCESI+DHMYRMG
Sbjct: 7 WKPEDNIPREILAILSKPHPNYQLAFLNIIQLLKTQRRTGWVDHGIDPCESISDHMYRMG 66
Query: 107 VTTMLIKDPNVDVKKCVRIALVHDIAESLVGDITPFDPVTKEEKHQRELDTITYLCEEII 166
+TTMLI D NVD KC+RIALVHD AESLVGDITP DP+TKEEKH+RE +T+ YLCE II
Sbjct: 67 LTTMLITDKNVDRNKCIRIALVHDFAESLVGDITPNDPMTKEEKHRREFETVKYLCESII 126
Query: 167 KPYNSVAADEILSDWLAYENISSLEARYVKDIDKFEMLVQCFEYEKRHSGTKDLSQFYGA 226
+P + A+ EIL DWLAYE + LE RYVKDIDK+EMLVQCFEYE++++G KDL QF GA
Sbjct: 127 RPCSESASREILDDWLAYEKQTCLEGRYVKDIDKYEMLVQCFEYEQKYNGKKDLKQFLGA 186
Query: 227 VSSIKTEEVKSWVSHLVKRRTEFFQQL 253
++ IKT+EVK W L++ R FF L
Sbjct: 187 INDIKTDEVKKWTQSLLEDRQAFFDSL 213
>SPCC4G3.17 [R] KOG3197 Predicted hydrolases of HD superfamily
Length = 198
Score = 180 bits (457), Expect = 1e-45
Identities = 87/182 (47%), Positives = 131/182 (71%)
Query: 69 VLAFLHIVEQLKLQKRTGWIDHDIIPCESIADHMYRMGVTTMLIKDPNVDVKKCVRIALV 128
++ FL + +LK RTGW+ H I ESIADHMYRMG+ TML DP+++ ++C++IA+V
Sbjct: 9 IVPFLDCLSRLKTTPRTGWLYHGIEKPESIADHMYRMGILTMLCNDPSINKERCLKIAVV 68
Query: 129 HDIAESLVGDITPFDPVTKEEKHQRELDTITYLCEEIIKPYNSVAADEILSDWLAYENIS 188
HD+AES+VGDITP + V+KEEKH+ E + + + +++I S+ A+EI +L YE+ S
Sbjct: 69 HDMAESIVGDITPHENVSKEEKHRMESEAMVSITQQLIPLNLSLQAEEIKELFLEYESAS 128
Query: 189 SLEARYVKDIDKFEMLVQCFEYEKRHSGTKDLSQFYGAVSSIKTEEVKSWVSHLVKRRTE 248
+ EA++VKDIDKFEM+ Q FEYE++ +G KDLSQF A I+ VK W++ +++ R +
Sbjct: 129 TPEAKFVKDIDKFEMIAQMFEYERKFNGEKDLSQFTWAGKLIQHPLVKGWLNDVLQEREQ 188
Query: 249 FF 250
F+
Sbjct: 189 FW 190
>At2g23820 [R] KOG3197 Predicted hydrolases of HD superfamily
Length = 243
Score = 131 bits (330), Expect = 8e-31
Identities = 75/193 (38%), Positives = 108/193 (55%), Gaps = 19/193 (9%)
Query: 55 AEVKELLSEPQPNYVLAFLHIVEQLKLQKRTGWIDHDIIPCESIADHMYRMGVTTMLIKD 114
A V S + + FL + +LK R GWI D+ ESIADHMYRMG+ ++ D
Sbjct: 65 AAVPSSSSSSSASSAIDFLSLCTRLKTTPRAGWIKRDVKDPESIADHMYRMGLMALISSD 124
Query: 115 -PNVDVKKCVRIALVHDIAESLVGDITPFDPVTKEEKHQRELDTITYLCEEIIKPYNSVA 173
P V+ KC+++A+VHDIAE++VGDITP ++KEEK++RE + + ++C+ +
Sbjct: 125 IPGVNRDKCMKMAIVHDIAEAIVGDITPSCGISKEEKNRRESEALEHMCKLL---GGGER 181
Query: 174 ADEILSDWLAYENISSLEARYVKDIDKFEMLVQCFEYEKRHSGTKDLSQFYGAVSSIKTE 233
A EI W YE SS EA+ VKD DK E+++Q EYE+ +T
Sbjct: 182 AKEIAELWREYEENSSPEAKVVKDFDKVELILQALEYEQ---------------GKFQTN 226
Query: 234 EVKSWVSHLVKRR 246
K+W S +V RR
Sbjct: 227 IGKAWASEIVSRR 239
>At1g26160 [R] KOG3197 Predicted hydrolases of HD superfamily
Length = 248
Score = 129 bits (323), Expect = 5e-30
Identities = 70/152 (46%), Positives = 93/152 (61%), Gaps = 5/152 (3%)
Query: 96 ESIADHMYRMGVTTMLIKD-PNVDVKKCVRIALVHDIAESLVGDITPFDPVTKEEKHQRE 154
ESIADHMYRM + ++ D VD ++C+++A+VHDIAE++VGDITP D V KEEK +RE
Sbjct: 92 ESIADHMYRMALMALIAGDLTGVDRERCIKMAIVHDIAEAIVGDITPSDGVPKEEKSRRE 151
Query: 155 LDTITYLCEEIIKPYNSVAADEILSDWLAYENISSLEARYVKDIDKFEMLVQCFEYEKRH 214
+ +CE + + A+EI WL YEN +SLEA VKD DK EM++Q EYE H
Sbjct: 152 TAALKEMCEVL---GGGLRAEEITELWLEYENNASLEANIVKDFDKVEMILQALEYEAEH 208
Query: 215 SGTKDLSQFYGAVSSIKTEEVKSWVSHLVKRR 246
D F +TE KSW + + RR
Sbjct: 209 GKVLD-EFFISTAGKFQTEIGKSWAAEINARR 239
>7297141 [R] KOG3197 Predicted hydrolases of HD superfamily
Length = 388
Score = 121 bits (304), Expect = 8e-28
Identities = 65/180 (36%), Positives = 109/180 (60%), Gaps = 7/180 (3%)
Query: 69 VLAFLHIVEQLKLQKRTGWIDHDIIPCESIADHMYRMGVTTMLIK-DPNVDVKKCVRIAL 127
+L F+ ++ LK KRTGW+ D+ CESI+ HMYRM + T L+ ++ +C+ +AL
Sbjct: 180 ILQFMELIGNLKHTKRTGWVLRDVNDCESISGHMYRMSMLTFLLDGSEGLNQIRCMELAL 239
Query: 128 VHDIAESLVGDITPFDPVTKEEKHQRELDTITYLCEEIIKPYNSVAADEILSDWLAYENI 187
VHD+AESLVGDITPF ++K++K E + +C ++I+P I+ + YE+
Sbjct: 240 VHDLAESLVGDITPFCGISKDDKRAMEFKAMEDIC-KLIEP----RGKRIMELFEEYEHG 294
Query: 188 SSLEARYVKDIDKFEMLVQCFEYEKRHSGTKDLSQFYGAV-SSIKTEEVKSWVSHLVKRR 246
+ E+++VKD+D+ +M++Q FEYEKR + +F+ + VK V+ + ++R
Sbjct: 295 QTAESKFVKDLDRLDMVMQAFEYEKRDNCLLKHQEFFDSTEGKFNHPFVKKLVNEIYEQR 354
>Hs7705624 [R] KOG3197 Predicted hydrolases of HD superfamily
Length = 170
Score = 73.9 bits (180), Expect = 2e-13
Identities = 54/180 (30%), Positives = 78/180 (43%), Gaps = 39/180 (21%)
Query: 69 VLAFLHIVEQLKLQKRTGWIDHDIIPCESIADHMYRMGVTTMLIKDPNVDVKKCVRIALV 128
+L FL +V QLK RTGW+ ++ ES++DHMYRM V M+IKD ++
Sbjct: 17 LLQFLRLVGQLKRVPRTGWVYRNVQRPESVSDHMYRMAVMAMVIKDDRLN---------- 66
Query: 129 HDIAESLVGDITPFDPVTKEEKHQRELDTITYLCEEIIKPYNSVAADEILSDWLAYENIS 188
K + IT L E ++ E+ W YE S
Sbjct: 67 ---------------------KDPEAMKQITQLLPEDLR-------KELYELWEEYETQS 98
Query: 189 SLEARYVKDIDKFEMLVQCFEYEKRHSGTKDLSQFYGAVS-SIKTEEVKSWVSHLVKRRT 247
S EA++VK +D+ EM++Q EYE L FY + + E+ VS L R+
Sbjct: 99 SAEAKFVKQLDQCEMILQASEYEDLEHKPGRLQDFYDSTAGKFNHPEIVQLVSELEAERS 158
>CE10532 [R] KOG3197 Predicted hydrolases of HD superfamily
Length = 158
Score = 73.9 bits (180), Expect = 2e-13
Identities = 54/181 (29%), Positives = 80/181 (43%), Gaps = 39/181 (21%)
Query: 69 VLAFLHIVEQLKLQKRTGWIDHDIIPCESIADHMYRMGVTTMLIKDP--NVDVKKCVRIA 126
+ L +++ LK KRTGW+ + E++A HMYRM V M ++ +D + V++A
Sbjct: 6 IFELLDVLDNLKHLKRTGWVKCGVPEPETVACHMYRMAVLAMALEGQIDGLDAIRTVKMA 65
Query: 127 LVHDIAESLVGDITPFDPVTKEEKHQRELDTITYLCEEIIKPYNSVAADEILSDWLAYEN 186
L + S V ++ +E W YE
Sbjct: 66 LAINTIASFVPNV----------------------------------GEEWTMLWKEYEE 91
Query: 187 ISSLEARYVKDIDKFEMLVQCFEYEKRHSGTKDLSQFY-GAVSSIKTEEVKSWVSHLVKR 245
SSL AR VK +DKF+M+VQ +YEK H DL QF+ V +K E +W L +
Sbjct: 92 ASSLTARVVKHLDKFDMIVQADKYEKTHE--IDLQQFFTSTVGVLKMEPFATWDRELREN 149
Query: 246 R 246
R
Sbjct: 150 R 150
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.135 0.402
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,471,540
Number of Sequences: 60738
Number of extensions: 641778
Number of successful extensions: 1629
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1614
Number of HSP's gapped (non-prelim): 9
length of query: 253
length of database: 30,389,216
effective HSP length: 104
effective length of query: 149
effective length of database: 24,072,464
effective search space: 3586797136
effective search space used: 3586797136
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)