ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI1855 suspect: LH K KOG4522 Transcription RNA polymerase II transcription mediator
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI1855 665638 661673 -1322
(1322 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YCR081w [K] KOG4522 RNA polymerase II transcription mediator 839 0.0
SPAC688.08 [K] KOG4522 RNA polymerase II transcription mediator 62 6e-09
>YCR081w [K] KOG4522 RNA polymerase II transcription mediator
Length = 1427
Score = 839 bits (2168), Expect = 0.0
Identities = 500/1410 (35%), Positives = 757/1410 (53%), Gaps = 122/1410 (8%)
Query: 23 QIYPDFDPWIHTKEEDKILKEFVAKGYYTASRVNFETISARSALQGSLPKVSSLLGDELS 82
Q+YPDF PW HT ED+IL FVAKG+Y VNFE+ISARS++ SL S++L +
Sbjct: 28 QVYPDFKPWEHTAAEDQILANFVAKGFYHTPMVNFESISARSSVHESLVTQSNILSQQFD 87
Query: 83 KXXXXXXXXXXXXSTLDIEDSTRFTKWSGQDFHLPNRVTLTDQKRNLWLQELSSSNTSLR 142
K ++ S T G F LPNR+TLTD ++ WL ELSSS+TSL
Sbjct: 88 KIIKIREDH------INKIPSNSTTTLHGPGFQLPNRITLTDHRKETWLHELSSSHTSLV 141
Query: 143 KLTKSVPHGFRKRHILEQCYTQFVPIYRVIWLIKSCYAIEWKGMISKAKGETT--HEELL 200
K+ K +PHG ++R ++EQCY +F+P+ R IWLIK CY IEWK K + + +
Sbjct: 142 KIGKFIPHGLKRRQVIEQCYLKFIPLKRAIWLIKCCYFIEWKSNHKKKRSNAAGADDAIS 201
Query: 201 STLYKDWTDNMVLILEKLVFEVSKYYNDPGQLKYWKLRIAHYLKILGNCYEMELLNRSTL 260
L KDWTD V ILEKL+F+++ +YND QL+ WK +I+++LK+LGNCY + L+N+
Sbjct: 202 MHLLKDWTDTFVYILEKLIFDMTNHYNDSQQLRTWKRQISYFLKLLGNCYSLRLINKEIF 261
Query: 261 HQWLVDFLSKIESFEFYPMTLHILSIFWSGI--------LGTTEEDTEIESSFLIIKLSE 312
H WLV+F++K+E+FEF P++LHIL IFW+ I + T ++ E FL+ K+++
Sbjct: 262 HHWLVEFINKMENFEFLPLSLHILMIFWNDICQIDTNAPVAATITSSQKEPFFLVTKITD 321
Query: 313 ILLRKYHIISESKCMINDSKYIINDVQRNSKLKESVLLRLRQFISHIFHTQSLEAFIMPK 372
+LL KY+I+S SK MIND YIIND+++N+K+K ++L L I IF QSLE FI P
Sbjct: 322 MLLHKYYIVSSSKSMINDENYIINDIKKNNKIKLNILKILSSLILKIFQEQSLEVFIFPT 381
Query: 373 QNWNIYKNYLYQILLQNVSDEETPXXXXXXXXISYRNESLRLGS----LDVEPSRETSPF 428
NW IYK L++I+ N + ISYRNESL+ S + + S
Sbjct: 382 SNWEIYKPLLFEIV-SNADTNQNSDMKKKLELISYRNESLKNNSSIRNVIMSASNANDFQ 440
Query: 429 STELTLDNVFSGNILNLKNVSPEXXXXXXXXXXXXEWSLFIDQKITKMEQVIEIILWAIN 488
T +T + + L + + +W ++DQ M ++I++ILW+I+
Sbjct: 441 LTIVTCKQFPKLSCIQLNCIDTQFTKLLDDNPTEFDWPTYVDQNPLTMHKIIQLILWSIH 500
Query: 489 PSRKH-RYDSCHLVAKILVLKINS-QESFQEYSIEDVIWSLIFHFSKLSKLELQKIVWL- 545
PSR+ Y+S LVAK+L+L+INS E E+ IED IWSL+F +K + + + ++
Sbjct: 501 PSRQFDHYESNQLVAKLLLLRINSTDEDLHEFQIEDAIWSLVFQLAKNFSAQKRVVSYMM 560
Query: 546 PKLHQLLNVFIGYGIIKVPTYIRRLISSGVLYLPESENKNFHCKLLINLKISPIMKSQYN 605
P L++LLN+ I YGIIKVPTYIR+LISSG+LYL +S +K H +LLINLKISP+MKSQYN
Sbjct: 561 PSLYRLLNILITYGIIKVPTYIRKLISSGLLYLQDSNDKFVHVQLLINLKISPLMKSQYN 620
Query: 606 MVLKNVMEYAPHFYEKYNFDKLVSIFESLKPKLLSGDFERLE--EYPGSIRIMCSEWYLS 663
MVL+NVMEY FYE +NFD+LV I E +K ++LS D L+ + P SI+IM +EWYLS
Sbjct: 621 MVLRNVMEYDVKFYEIFNFDQLVEITEQIKMRILSNDITNLQLSKTPLSIKIMVAEWYLS 680
Query: 664 QICFNQGELQKVNNHKIIKTFQLFCINLGELHHFFGWAEYIVYHQLIDDLENLEVFTDIL 723
+C G L VN ++K F++FCI+L HHFF W E+IVYHQL+ D+E+LE DIL
Sbjct: 681 HLC--SGILSSVNRTVLLKIFKIFCIDLEVFHHFFKWIEFIVYHQLLSDIESLEALMDIL 738
Query: 724 LYLDKAFLLLINDHILFMKTLLHSYMRDLRIKDKAAFELINFKSFWKFFVKNCANMIEMD 783
L K F INDHILF KT + Y + L+ KD A+ + +F FWKFF+KN ++++D
Sbjct: 739 LCYQKLFSQFINDHILFTKTFIFIYKKVLKEKDVPAYNVTSFMPFWKFFMKNFPFVLKVD 798
Query: 784 SSLQNQIAEVFELERHRKDHFTKMPNETVSLYLEITGNKNTKFEEQNFPSVIQQNIKKVL 843
+ L+ ++ V+ E+ + + +E + +Y I + + NFP V Q NI+ +L
Sbjct: 799 NDLRIELQSVYNDEKLKTEKLKNDKSEVLKVYSMINNSNQAVGQTWNFPEVFQVNIRFLL 858
Query: 844 HNPE--------DESNCRKLLLLCKASHASEYNKFLSIFVKRGDFTVHEXXXXXXXXXXX 895
HN E R ++L A++ EYNKF+SIF+KR DFT
Sbjct: 859 HNSEIIDTNTSKQFQKARNNVMLLIATNLKEYNKFMSIFLKRKDFTNKNLIQLISLKLLT 918
Query: 896 FEVIQKTSHFDLLCEL----VSQVSFNYGLNFENEKNAFVKRNFKQITLQLFS------- 944
FEV Q + + L + +YGL + K F+K NF++I L +
Sbjct: 919 FEVTQNVLGLEYIIRLLPINLENNDGSYGLFLKYHKEQFIKSNFEKILLTCYELEKKYHG 978
Query: 945 ---RPTFRDTLVRMLVEYGPNSNLSEKSAQVVSHILRE--EKNMSIIRDMLIYGM----- 994
+ + L+++L+ YG + L S +++ +L + E + +I+ D+L Y
Sbjct: 979 NECEINYYEILLKILITYGSSPKLLATSTKIIMLLLNDSVENSSNILEDILYYSTCPSET 1038
Query: 995 ----------NIENEAI----------DNTID------LYRYLNFTNTWLFQILTEFNVK 1028
+N+ + D+T+D Y ++F N W+FQ T F +K
Sbjct: 1039 DLNDIPLGSGQPDNDTVVTNDDKSDDDDHTVDEIDHVEYYVMMDFANLWVFQAFTCFCIK 1098
Query: 1029 -----NSNA---EDLSDFFSSIVGAIGYNSLLPKIFSNISDKKQVSTLLTVIERQFLARV 1080
N A EDL +F I+ N L +IF + D + + + ++E+ F
Sbjct: 1099 KIMENNEPAMAMEDLKNFIFQIIEITNSNDLCSQIFDQLKDMQTIEMITQIVEKDFCTSC 1158
Query: 1081 LETEKSALVFLHIMMDTEIVLS------RHLVNMSSLGMDSEVLELFKNTIATFAKMPND 1134
L+ + +I++ EI+ S R M + M++ L I +++
Sbjct: 1159 LQNNNQKIDDNYIVVVIEIITSLSMRFQRETSGMIVISMEN--YHLLIKIIRQLSELNEG 1216
Query: 1135 TLKKYDENLNELLKIVIIHEKFILRHCFE--SIELRDHYMVDNFYTLFHNTG-XXXXXXX 1191
L K + ++ +LKI H+ I + S + +D+ LF
Sbjct: 1217 NLSKREIQIDAVLKIFSFHQDSIFQRIIADLSADKPTSPFIDSICKLFDKISFNLRLKLF 1276
Query: 1192 XXXXXXXXXXXXILNETKD-QALHS---IKMPSVLNQLPKFAISSFL------DSEGTED 1241
I + T D A H+ +++P L LP F +SSF+ + E+
Sbjct: 1277 LYEILSSLKSFAIYSSTIDAPAFHTSGKVELPKKLLNLPPFQVSSFVKETKLHSGDYGEE 1336
Query: 1242 EFLDKDEPSLIRLGICDKQAKTNSALKYFVFNKKSSQFDCIFKIEPFQYLVNYQEPLEGE 1301
E D++E + LGI + + K+ +++KK ++ C F +EP+ ++ NY +
Sbjct: 1337 EDADQEESFSLNLGIGIVEIAHENEQKWLIYDKKDHKYVCTFSMEPYHFISNYNTKYTDD 1396
Query: 1302 L----------NNTSLSLGLFDARLEKTNP 1321
+ N++ ++L LFDAR E+ NP
Sbjct: 1397 MATGSNDTTAFNDSCVNLSLFDARFERKNP 1426
>SPAC688.08 [K] KOG4522 RNA polymerase II transcription mediator
Length = 1233
Score = 62.0 bits (149), Expect = 6e-09
Identities = 64/243 (26%), Positives = 101/243 (41%), Gaps = 24/243 (9%)
Query: 45 VAKGYYTASRVNFETISARSALQGSLPKVSSLLGDELSKXXXXXXXXXXXXSTLDIEDST 104
++KG+ + V + SA+ LQ L K S+L + L + STL +
Sbjct: 64 ISKGHRYSPPVRSDRASAQGLLQQHLEKSSTL--ENLKRYS----------STLLGRQND 111
Query: 105 RFTKWSGQDFHLPNRVTLTDQKRNLWLQELSSSNTSLRKLTKSVPHGFRKRHILEQCYTQ 164
F P RVTL++ +R+ WLQ LS L L K++PHG IL
Sbjct: 112 AVIDEPFAPFRPPPRVTLSESRRDRWLQGLSDPMVPLSSLIKTIPHGLWGEDILRMLVKF 171
Query: 165 FVPIYRVIWLIKSCYAIEWKGMISKAKGETTHEELLSTLYKDWTDNMVLILEKLVFEVSK 224
VP R IW I+ E + + K +TT ++ K+WTD + L+ +S
Sbjct: 172 RVPFTRAIWFIRCAGVNEARSFLRKV--QTTD---ITEWVKNWTD---VAAGFLISFISS 223
Query: 225 YYNDPGQLKYWKLRIAHYLKILGNCYEMELLNRSTLHQWLVDFL--SKIESFEFYPMTLH 282
+ N + + + LK+ G EL++ +V F S ++SF + L
Sbjct: 224 FLN--ADIYSFADDYTYLLKLFGRLLAEELVSPKHFLLRIVSFSGDSSLKSFSLHFFALQ 281
Query: 283 ILS 285
S
Sbjct: 282 FFS 284
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.321 0.137 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 74,489,411
Number of Sequences: 60738
Number of extensions: 3132429
Number of successful extensions: 7942
Number of sequences better than 1.0e-05: 2
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 7923
Number of HSP's gapped (non-prelim): 2
length of query: 1322
length of database: 30,389,216
effective HSP length: 118
effective length of query: 1204
effective length of database: 23,222,132
effective search space: 27959446928
effective search space used: 27959446928
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)