ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactVI1920   suspect: LH   OT    KOG0045  Posttranslational modification, protein turnover, chaperones   Cytosolic Ca2+-dependent cysteine protease (calpain), large subunit (EF-Hand protein superfamily)
r_klactVI1920   suspect: LH   OT    KOG0045  Signal transduction mechanisms                                 Cytosolic Ca2+-dependent cysteine protease (calpain), large subunit (EF-Hand protein superfamily)

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactVI1920 684265  686319 685  
         (685 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YMR154c [OT] KOG0045 Cytosolic Ca2+-dependent cysteine protease ...   173   7e-43

>YMR154c [OT] KOG0045 Cytosolic Ca2+-dependent cysteine protease (calpain)
           large subunit (EF-Hand protein superfamily)
          Length = 727

 Score =  173 bits (439), Expect = 7e-43
 Identities = 168/660 (25%), Positives = 284/660 (42%), Gaps = 57/660 (8%)

Query: 47  KSDDKQLIRFLIWFNDARKRSNFNDKFQWMTSFLYGRRYPPLGDDLVVKEPWNDLQQLDS 106
           KS+D   I  ++   +   + +   +F W+TS +  R YPP+         WN  +    
Sbjct: 35  KSEDSTFIEAVLVLKENVSKVDKQLRFLWLTSTINSRFYPPIPISEASPVSWNKTEYCAP 94

Query: 107 IIKYGNEPIDL-----VKMQPDFTTEERIVQNRRVNNCSLICSLTNAREEN-------QI 154
               G E +        K+Q +      I Q R V +CSL+ SL N R +N       QI
Sbjct: 95  ----GTEELQRRYPGRAKLQNEEDYSGGIEQCRDVPDCSLVASLINLRSKNLNLPLIKQI 150

Query: 155 KSYSVHNVFNVILHFNGSNRLVSVLRDSNFPKCSKDGAALTLFSHNIEDMLIELAYFEIK 214
            S   H    V L FNGSN+ +  +  S  P  S DG  L+L S++I D + ELA   + 
Sbjct: 151 SSTKYH----VNLSFNGSNKRLVTVDISQIPT-SVDGKQLSLKSNDISDKIGELALLLVS 205

Query: 215 NNRNYAYGGSNTATDTYLLTGWIPEILNLGDVSFSEIRKCYQFN-AMLALGTDSVKG--- 270
               Y+  GSN + DTY L+G++PEI  +    F ++ K ++ N  ++  GT +      
Sbjct: 206 KG-TYSTDGSNISIDTYRLSGFLPEITQVNSYPFEKLWKFHKSNLCLMGAGTGNRSNDMI 264

Query: 271 KEVTENHDFVVTSVNWKKEQFTILNPHGTENPTVYTWDDLIKEFKWLYVNWDPYSKYTQR 330
           K + ENHD+ +  + +      + +P  +      +++  +K FK LY+NW+    + + 
Sbjct: 265 KPLVENHDYSIIDITYDSRLVKLRDPRNSALNVEISYEQYLKNFKQLYLNWNQEKLFKRS 324

Query: 331 VKLHDRY-PDKFNAYPTWTQKPLMCIENKSNSIQNCHLYLERHLLDDSILESNLT--IGE 387
             LH RY   ++N +     KPL  + N S   +   L LE HL D+   E+     + E
Sbjct: 325 QVLHFRYDTSRYNKFSIVADKPLFHLVNNSKVTETVWLLLESHLQDEGSQENRSVSFLNE 384

Query: 388 IPRSGF----TAVSAEGNNSGFCNMQLRLNPHETRLIFYHSDKKCNLTFHLLSNSGLVSM 443
            P          V   GN+ G   ++LRL+    RL++ +S    N + H  S    +  
Sbjct: 385 APECIICPIEPPVECGGNHIGLQLVKLRLDAETERLLYCYSTTNNNFSIHSFSVVKEICF 444

Query: 444 SKCK-SDILTAESSWNI---TNNQWTIGTSEYFKSPVFKLKSDGFDD--TYLDFELVSQL 497
            + K +  L A+  ++       + +  T  +F++P F+L+     D    +D   +S  
Sbjct: 445 QRLKDTKSLFAKVLFSFPYEIEGKASFDTCNFFQNPTFELEVHSEQDYQVLMDAACISTS 504

Query: 498 PAK-VNFQIFESDDYALQRPIFKNDTYSPQIFSKHGVRLLKGREYFIVCSIAHHITSPFI 556
               +N Q++  +DY L +PI  ++ Y P    K  V +L   +Y IVCS      S   
Sbjct: 505 SHDLINIQVYYFNDYELIKPIMFDNHYQPGQGLKQDVPILTNVKYMIVCSTYGPPASTEF 564

Query: 557 FTLRNPETKQSKFELSPYSLGFGGLRYNEV-LRCKTR-------------ITISVSNFT- 601
             L +     S   +S  +L    L Y      C+ R             +T+    ++ 
Sbjct: 565 ELLASIRLSSSWRLISGITLRSVNLIYGTYPYHCRNRFHWKETSDKLKIQMTLPTKKYST 624

Query: 602 -RLFVLLFTSASKTNGRLTLRLYEKETEEILFEQIETRYDLSVKPFPIPNIELKPASYVL 660
            +LF+ +    S    R+   ++E E+   ++E  E R   S     IP++E+   + V+
Sbjct: 625 NKLFIRVVPVESSARLRMRCNIFEPESALCVYECQEYRTCPS-GGIVIPDLEVSRTNIVV 683


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.320    0.137    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,549,847
Number of Sequences: 60738
Number of extensions: 1976508
Number of successful extensions: 4132
Number of sequences better than 1.0e-05: 1
Number of HSP's better than  0.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 4124
Number of HSP's gapped (non-prelim): 1
length of query: 685
length of database: 30,389,216
effective HSP length: 113
effective length of query: 572
effective length of database: 23,525,822
effective search space: 13456770184
effective search space used: 13456770184
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)