ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI1990 good Q KOG0021 Secondary metabolites biosynthesis, transport and catabolism Glutathione synthetase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI1990 713070 711610 -487
(487 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOL049w [Q] KOG0021 Glutathione synthetase 521 e-148
SPAC13F10.04 [Q] KOG0021 Glutathione synthetase 328 1e-89
At5g27380 [Q] KOG0021 Glutathione synthetase 263 3e-70
Hs4504169 [Q] KOG0021 Glutathione synthetase 251 1e-66
CE18087 [Q] KOG0021 Glutathione synthetase 206 6e-53
7293404 [Q] KOG0021 Glutathione synthetase 70 9e-12
>YOL049w [Q] KOG0021 Glutathione synthetase
Length = 491
Score = 521 bits (1342), Expect = e-148
Identities = 263/479 (54%), Positives = 350/479 (72%), Gaps = 14/479 (2%)
Query: 22 LQSEIFRWALTNGLAMYPPNFK--PELATVAPITLFPTPLPNEAFQKAIDVQTIYNALYA 79
L E+ +WA+TNGL+MYPP F+ P A+V+P+T++PTP+P + F +A+ +Q ++N LYA
Sbjct: 14 LIQEVNQWAITNGLSMYPPKFEENPSNASVSPVTIYPTPIPRKCFDEAVQIQPVFNELYA 73
Query: 80 SISQNK---NDWLAKETEKLAKSDPEFTGRLWTLYK---RAKEYGIVQDLALGVFRSDYL 133
I+Q+ + +L K TE LA SD EFTG+LW+LY ++ +Y Q+ LG+FRSDYL
Sbjct: 74 RITQDMAQPDSYLHKTTEALALSDSEFTGKLWSLYLATLKSAQYK-KQNFRLGIFRSDYL 132
Query: 134 INSDNN--QIKQVEFNTVSVSFGGLSTKVGELHDYLNKSGNYSDNGEVFYDQDIPISPSA 191
I+ QIKQVEFNTVSVSF GLS KV LH YLN++ Y G ++ DQ++ IS S
Sbjct: 133 IDKKKGTEQIKQVEFNTVSVSFAGLSEKVDRLHSYLNRANKYDPKGPIYNDQNMVISDSG 192
Query: 192 ELLATGLATAINKY---QPADKNKRPIVAFIVQNGERNVFDQSILALNLLSKYDTKSVRL 248
LL+ LA A+ Y Q + PIVAFIVQ ERNVFDQ +L LNLL K+ TKSVRL
Sbjct: 193 YLLSKALAKAVESYKSQQSSSTTSDPIVAFIVQRNERNVFDQKVLELNLLEKFGTKSVRL 252
Query: 249 TIHEIHEATRLDPETKRLYHKTTGQEIGLVYFRSGYAPSDFVSEQDWENRLTLEISYAIK 308
T ++++ +D +T +L+ + T QEI +VY+R+GY +D+ SE+DWE RL LE S+AIK
Sbjct: 253 TFDDVNDKLFIDDKTGKLFIRDTEQEIAVVYYRTGYTTTDYTSEKDWEARLFLEKSFAIK 312
Query: 309 APNLLTQLSGTKKIQQLLTDPKILKEFLSDTESLDHLLPTFVKIYPLDSSVLGEIGKKLA 368
AP+LLTQLSG+KKIQQLLTD +L +++SD E LL TFVKIYPLD + LG GK+LA
Sbjct: 313 APDLLTQLSGSKKIQQLLTDEGVLGKYISDAEKKSSLLKTFVKIYPLDDTKLGREGKRLA 372
Query: 369 LTEPENYVLKPQREGGGNNIYKEDIPGFLESVDEDEWSAYILMELITPKSTEENFVIRGQ 428
L+EP YVLKPQREGGGNN+YKE+IP FL+ ++E W AYILMELI P+ E N ++R
Sbjct: 373 LSEPSKYVLKPQREGGGNNVYKENIPNFLKGIEERHWDAYILMELIEPELNENNIILRDN 432
Query: 429 ELFNVPILSELGIFGTILFDHNNIYSNEYAGWLLRSKFSSSNEGGVAAGFGCVDSVVLY 487
+ +N PI+SELGI+G +LF+ + SNE++G LLRSKF++SNEGGVAAGFGC+DS++LY
Sbjct: 433 KSYNEPIISELGIYGCVLFNDEQVLSNEFSGSLLRSKFNTSNEGGVAAGFGCLDSIILY 491
>SPAC13F10.04 [Q] KOG0021 Glutathione synthetase
Length = 498
Score = 328 bits (840), Expect = 1e-89
Identities = 187/464 (40%), Positives = 284/464 (60%), Gaps = 31/464 (6%)
Query: 46 LATVAPITLFPTPLPNEAFQKAIDVQTIYNALYASISQNKNDWLAKETEKLAKSDPEFTG 105
+AT PITLFP+ +P+ AF +A+ VQ YN LYA I+ N ++L + + K D EF
Sbjct: 41 IATQIPITLFPSVIPHGAFVEAVSVQKAYNKLYAKIA-NDYEFLRLHLQSITKYD-EFMN 98
Query: 106 RLWTLYKRAKEY------GIVQDLALGVFRSDYLINSDNNQI--KQVEFNTVSVSFGGLS 157
+LW LY++ +E Q L+LGVFRSDY+++ D++ I KQVEFNT+SVSFGG+S
Sbjct: 99 KLWNLYQKHREAVAHLKENQFQPLSLGVFRSDYMVHQDDSFIGCKQVEFNTISVSFGGVS 158
Query: 158 TKVGELHDYLNKSGNYSDNGEVFYDQDIPISPSAELLATGLATAINKYQP---------- 207
V LH Y ++SG Y Y + ++ S + TG++ A++ Y+
Sbjct: 159 KAVSNLHAYCSQSGLYRKPLTTNY---LTVNTSVSGICTGISNAVDAYRDYVKNITSKMN 215
Query: 208 -ADKNKRPIVAFIVQNGERNVFDQSILALNLLSKYDTKSVRLTIHEIHEATRLDPETKRL 266
A N +PIV F+V+ GERN+ DQ L LL+++ S R+ I E++ D + +L
Sbjct: 216 IASDNTKPIVLFVVKGGERNITDQRTLEYELLNRFHVISKRIDIAELNSLIH-DKSSNKL 274
Query: 267 YHKT--TGQEIGLVYFRSGYAPSDFVSEQDWENRLTLEISYAIKAPNLLTQLSGTKKIQQ 324
Y KT T E+ +VY+R GYA D+ S++ W+ RLT+E + AIK P++ T L+G+KKIQQ
Sbjct: 275 YMKTSFTTYEVAVVYYRVGYALDDYPSQEAWDMRLTIENTLAIKCPSISTHLAGSKKIQQ 334
Query: 325 LLTDPKILKEFLSDTESLDHLLPTFVKIYPLDSSVLGEIGKKLALTEPENYVLKPQREGG 384
+L + L+ FL E L + TF +YPLD + G+ G KLA +PE++VLKPQREGG
Sbjct: 335 VLAESNALERFLEGDE-LQAVRSTFADMYPLDDTPRGKEGIKLAFEKPEDFVLKPQREGG 393
Query: 385 GNNIYKEDIPGFLESVDEDEWSAYILMELITPKSTEENFVIRGQELFNVPILSELGIFGT 444
GNN Y +DIPG L + ++EW +YILM I + +N++++G+ ++ E+GI GT
Sbjct: 394 GNNTYGKDIPGLLSKMPQEEWDSYILMRYINAVPS-QNYILKGERPEKFDVVDEIGILGT 452
Query: 445 ILFD--HNNIYSNEYAGWLLRSKFSSSNEGGVAAGFGCVDSVVL 486
I+++ + + N +G++ R+K +NEGGVA G+ + S+ L
Sbjct: 453 IVWNIKTDEVVQNGQSGFICRTKPKKTNEGGVATGYASLSSIEL 496
>At5g27380 [Q] KOG0021 Glutathione synthetase
Length = 539
Score = 263 bits (673), Expect = 3e-70
Identities = 172/490 (35%), Positives = 270/490 (55%), Gaps = 32/490 (6%)
Query: 11 PAISTKKLQDGLQSEIFR----WALTNGLAMYPPNFK-----PELATV-APITLFPTPLP 60
P +KL D ++ W+ +GL + +++ P + + API L PT P
Sbjct: 67 PIFDLEKLDDEFVQKLVYDALVWSSLHGLVVGDKSYQKSGNVPGVGLMHAPIALLPTAFP 126
Query: 61 NEAFQKAIDVQTIYNALYASISQNKNDWLAKETEKLAKSDPEFTGRLWTLYKRAKEYGIV 120
+++A +V ++N L +S + +L + K D FT RL ++ + E
Sbjct: 127 EAYWKQACNVTPLFNELIDRVSLD-GKFLQDSLSRTKKVDV-FTSRLLDIHSKMLERNKK 184
Query: 121 QDLALGVFRSDYLINSDNNQIKQVEFNTVSVSFGGLSTKVGELHDYLNKSGNYSDNGEVF 180
+D+ LG+ R DY+++ + N + Q+E NT+S SF GLS V +LH L +S Y D ++
Sbjct: 185 EDIRLGLHRFDYMLDEETNSLLQIEMNTISCSFPGLSRLVSQLHQSLLRS--YGD--QIG 240
Query: 181 YDQD-IPISPSAELLATGLATAINKYQPADKNKRPIVAFIVQNGERNVFDQSILALNLLS 239
D + +PI+ S A LA A +Y N R +V IVQ ERN++DQ +L+ L
Sbjct: 241 IDSERVPINTSTIQFADALAKAWLEYS----NPRAVVMVIVQPEERNMYDQHLLSSILRE 296
Query: 240 KYDTKSVRLTIHEIHEATRLDPETKRLYHKTTGQEIGLVYFRSGYAPSDFVSEQDWENRL 299
K++ +R T+ E+ + + + + GQ + +VYFRSGY P+D SE +W RL
Sbjct: 297 KHNIVVIRKTLAEVEKEGSVQEDETLI---VGGQAVAVVYFRSGYTPNDHPSESEWNARL 353
Query: 300 TLEISYAIKAPNLLTQLSGTKKIQQLLTDPKILKEFLSDTESLDHLLPTFVKIYPLDSSV 359
+E S A+K P++ L+G+KKIQQ L P +L+ FL + E + L F ++ LD S
Sbjct: 354 LIEESSAVKCPSIAYHLTGSKKIQQELAKPGVLERFLDNKEDIAKLRKCFAGLWSLDDSE 413
Query: 360 LGEIGKKLALTEPENYVLKPQREGGGNNIYKEDIPG---FLESVDEDEWSAYILMELITP 416
+ K A+ +P +V+KPQREGGGNNIY +D+ L+ E+ +AYILM+ I P
Sbjct: 414 I----VKQAIEKPGLFVMKPQREGGGNNIYGDDVRENLLRLQKEGEEGNAAYILMQRIFP 469
Query: 417 KSTEENFVIRGQELFNVPILSELGIFGTILFDHNNIYSNEYAGWLLRSKFSSSNEGGVAA 476
K F++R +SELG++G L + + NE +G+L+R+K +SS+EGGVAA
Sbjct: 470 K-VSNMFLVREGVYHKHQAISELGVYGAYLRSKDEVIVNEQSGYLMRTKIASSDEGGVAA 528
Query: 477 GFGCVDSVVL 486
GFG +DS+ L
Sbjct: 529 GFGVLDSIYL 538
>Hs4504169 [Q] KOG0021 Glutathione synthetase
Length = 474
Score = 251 bits (642), Expect = 1e-66
Identities = 168/445 (37%), Positives = 252/445 (55%), Gaps = 23/445 (5%)
Query: 45 ELATVAPITLFPTPLPNEAFQKAIDVQTIYNALYASISQNKNDWLAKETEKLAKSDPEFT 104
E+ + AP TLFP+ +P+ ++A VQ +N L ++SQN +L + K D +FT
Sbjct: 43 EVVSYAPFTLFPSLVPSALLEQAYAVQMDFNLLVDAVSQNAA-FLEQTLSSTIKQD-DFT 100
Query: 105 GRLWTLYKRAKEYGIVQDLALGVFRSDYLINSDNN---QIKQVEFNTVSVSFGGLSTKVG 161
RL+ ++K+ + GI Q + LG+ RSDY+ + +KQ+E NT+S SFGGL+++
Sbjct: 101 ARLFDIHKQVLKEGIAQTVFLGLNRSDYMFQRSADGSPALKQIEINTISASFGGLASRTP 160
Query: 162 ELHDY-LNKSGNYSDNGEVFYDQDIPISPSAELLATGLATAINKYQPADKNKRPIVAFIV 220
+H + L+ + G++ + +PS L A G+A A Y + +V I
Sbjct: 161 AVHRHVLSVLSKTKEAGKILSN-----NPSKGL-ALGIAKAWELYG----SPNALVLLIA 210
Query: 221 QNGERNVFDQSILALNLLSKYDTKSVRLTIHEIHEATRLDPETKRLYHKTTGQEIGLVYF 280
Q ERN+FDQ + LL++ + +R T +I E LD + +RL+ GQEI +VYF
Sbjct: 211 QEKERNIFDQRAIENELLAR-NIHVIRRTFEDISEKGSLDQD-RRLF--VDGQEIAVVYF 266
Query: 281 RSGYAPSDFVSEQDWENRLTLEISYAIKAPNLLTQLSGTKKIQQLLTDPKILKEFL-SDT 339
R GY P + S Q+WE RL LE S+A K P++ TQL+GTKK+QQ L+ P +L+ L
Sbjct: 267 RDGYMPRQY-SLQNWEARLLLERSHAAKCPDIATQLAGTKKVQQELSRPGMLEMLLPGQP 325
Query: 340 ESLDHLLPTFVKIYPLDSSVLGEIGKKLALTEPENYVLKPQREGGGNNIYKEDIPGFLES 399
E++ L TF +Y LD G+ AL P +VLKPQREGGGNN+Y E++ L+
Sbjct: 326 EAVARLRATFAGLYSLDVGEEGDQAIAEALAAPSRFVLKPQREGGGNNLYGEEMVQALKQ 385
Query: 400 V-DEDEWSAYILMELITPKSTEENFVIRGQELFNVPILSELGIFGTILFDHNNIYSNEYA 458
+ D +E ++YILME I P+ E + G V +SELGIFG + + N++
Sbjct: 386 LKDSEERASYILMEKIEPEPFENCLLRPGSPARVVQCISELGIFGVYVRQEKTLVMNKHV 445
Query: 459 GWLLRSKFSSSNEGGVAAGFGCVDS 483
G LLR+K +GGVAAG +D+
Sbjct: 446 GHLLRTKAIEHADGGVAAGVAVLDN 470
>CE18087 [Q] KOG0021 Glutathione synthetase
Length = 490
Score = 206 bits (524), Expect = 6e-53
Identities = 147/491 (29%), Positives = 254/491 (50%), Gaps = 28/491 (5%)
Query: 8 PKFPAISTKKLQDGLQSEIFRWALTNGLAMYPPNFK--PELATVAPITLFPTPLPNEAFQ 65
P+ P + KL + L +++ WA NGL M K E+ P+TL P+P P F+
Sbjct: 14 PRLP-LEDDKLNE-LTADLHDWAHANGLVMRLSTDKLSSEVCQTTPLTLLPSPFPKNVFE 71
Query: 66 KAIDVQTIYNALYASISQNKNDWLAKETEKLAKSDPEFTGRLWTLYKRAKEYGIVQDLAL 125
+A+ +Q ++ +LY I+ + D+L + + K+D +FT + + K+ K G+ Q + L
Sbjct: 72 EAVHIQNLFASLYHFIAY-EFDFLIDIHKNVVKTD-DFTRNMVEILKKVKAQGLKQPVTL 129
Query: 126 GVFRSDYLINSDNNQ----IKQVEFNTVSVSFGGLSTKVGELHDYLNKSGNYSDNGEVFY 181
+ RSDY+ + D +KQ+E N ++ S G + ++ E H + K+ N SD+
Sbjct: 130 AIQRSDYMCHKDQYSAEYGLKQIEINNIASSMGAHALRLTEWHIRVLKALNISDD---VI 186
Query: 182 DQDIPISPSAELLATGLATAINKYQPADKNKRPIVAFIVQNGERNVFDQSILALNL--LS 239
+ IP + ++A L A + + N +V +V+N +N DQ + L L
Sbjct: 187 QRAIPENKPIPMIAEALFKAWSHFS----NPAAVVLVVVENVNQNQIDQRHVEYELEKLG 242
Query: 240 KYDTKSVRLTIHEIHEATRLDPETKRLYHKTTGQEIGLVYFRSGYAPSDFVSEQDWENRL 299
T +R + + +E L+ + + G+++ +VYFR+GY+P + S ++WE R
Sbjct: 243 VPMTCIIRRNLTQCYEQLSLNDRSDLMID---GRQVAIVYFRAGYSPDHYPSTKEWEARE 299
Query: 300 TLEISYAIKAPNLLTQLSGTKKIQQLLTDPKILKEFLSDTESLDHLLPTFVKIYPLDSSV 359
+E+S AIK P + Q++ TKK QQ+L++ +L+ F+ + +F ++ L+++
Sbjct: 300 RMELSTAIKTPWIGLQVANTKKTQQVLSEDGVLERFIGKPREARDIRASFAGMWALENT- 358
Query: 360 LGEIGKKL---ALTEPENYVLKPQREGGGNNIYKEDIPGFLESVDEDEWSAYILMELITP 416
E+ K+ A PE +VLKPQ EGG +++ L + E+E A+ILME + P
Sbjct: 359 -DEVTMKVVAGAQKHPEAFVLKPQTEGGAALHTGDEMVQMLRELPEEERGAFILMEKLKP 417
Query: 417 KSTEENFVIRGQELFNVPILSELGIFGTILFDHNNIYSNEYAGWLLRSKFSSSNEGGVAA 476
E V+ + + +SELG++G F + + AG LLR+K S+ GGVAA
Sbjct: 418 MIIENYLVLAKKPITFAKAVSELGVYG-YAFGRKDAPELKTAGHLLRTKPESTAMGGVAA 476
Query: 477 GFGCVDSVVLY 487
G VD+ LY
Sbjct: 477 GHAVVDTPFLY 487
>7293404 [Q] KOG0021 Glutathione synthetase
Length = 99
Score = 69.7 bits (169), Expect = 9e-12
Identities = 41/85 (48%), Positives = 56/85 (65%), Gaps = 2/85 (2%)
Query: 404 EWSAYILMELITPKSTEENFVIRGQELFN--VPILSELGIFGTILFDHNNIYSNEYAGWL 461
E SA+ILM+LI P T+ V G ++ V ++SELGIFG ++ D +I N AG +
Sbjct: 5 ERSAWILMDLIHPPLTKGYMVRPGGDMPPQIVDMVSELGIFGVVIGDAEHIVHNYQAGHM 64
Query: 462 LRSKFSSSNEGGVAAGFGCVDSVVL 486
LR+K S++NEGGVAAG G +DS L
Sbjct: 65 LRTKLSTANEGGVAAGLGALDSPYL 89
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.135 0.387
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,956,578
Number of Sequences: 60738
Number of extensions: 1335093
Number of successful extensions: 2613
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2576
Number of HSP's gapped (non-prelim): 6
length of query: 487
length of database: 30,389,216
effective HSP length: 110
effective length of query: 377
effective length of database: 23,708,036
effective search space: 8937929572
effective search space used: 8937929572
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)