ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactVI2089   suspect: LH   S     KOG4067  Function unknown                                               Uncharacterized conserved protein

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactVI2089 746451 745726 -242 
         (242 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YOL032w [S] KOG4067 Uncharacterized conserved protein                 312   3e-85
SPBC21H7.06c [S] KOG4067 Uncharacterized conserved protein             72   5e-13

>YOL032w [S] KOG4067 Uncharacterized conserved protein
          Length = 246

 Score =  312 bits (799), Expect = 3e-85
 Identities = 167/252 (66%), Positives = 195/252 (77%), Gaps = 16/252 (6%)

Query: 1   MFAAIASGNPLQLSTEVPNSNGLQHTIVLSSTKPKSYSHITLFILPNVTFPQEFLATVYF 60
           MFAAIASGNPLQLS EVPNSNGLQHTIVLS TKPK YSHITLFILPNVTFPQ+++ATVYF
Sbjct: 1   MFAAIASGNPLQLSVEVPNSNGLQHTIVLSRTKPKLYSHITLFILPNVTFPQDYIATVYF 60

Query: 61  KLNPAEDFKLFGYLGNEKPSAIFKVKLPQANVGNGVTANPSDGLGEIDMEDDVMT----- 115
           KL+P E+FKLFGYL +EKPSAIFKV++P +    G T   SDGLGEIDM+ D  +     
Sbjct: 61  KLSPQEEFKLFGYLSSEKPSAIFKVQIPSSKKDAGDT---SDGLGEIDMDVDDGSGAADP 117

Query: 116 -DDLNGANANHNISQLIIGISIEPRNDATAKLEQWKAEQXXXXXXXXXXXMVLSKNSVQI 174
             D NG+++N NIS+LIIGISIEPR     KLE+WKA             ++LS+ ++ I
Sbjct: 118 FTDTNGSSSN-NISELIIGISIEPREQGMMKLEEWKASM--NAEAQKNNSLILSRPNLGI 174

Query: 175 L----TAGALAKQYPVVTQELAAKIVQHAYNYLTGFVDAQGNVSIKRFDTWWEKFRVRLQ 230
           +    TAG LA+ YP +TQELAAKIVQHAYNYL+GF+DAQGNV IKRFDTWW+KFR RL 
Sbjct: 175 IRNITTAGQLAQVYPSLTQELAAKIVQHAYNYLSGFLDAQGNVPIKRFDTWWDKFRNRLA 234

Query: 231 NDATFLDEVTKD 242
           ND TFLDEVTK+
Sbjct: 235 NDGTFLDEVTKN 246


>SPBC21H7.06c [S] KOG4067 Uncharacterized conserved protein
          Length = 200

 Score = 72.4 bits (176), Expect = 5e-13
 Identities = 70/252 (27%), Positives = 101/252 (39%), Gaps = 72/252 (28%)

Query: 1   MFAAIASGNPLQLSTEVPNSNGLQHTIVLSSTKPKSYSHITLFILPNVTFPQEFLATVYF 60
           MF AI +G  +Q + +    N      V      +S +HI +F+LPN  FP    A VYF
Sbjct: 1   MFGAICAGRLVQTNLQQVADN----QFVFQLDSAESLNHIVVFLLPNSPFPVGMGAKVYF 56

Query: 61  KLNPAEDFKLFGYLGNEKPSAIFKVKLPQANVGNGVTANPSDGLGEIDMEDDVMTDDLNG 120
           +  P + F+  GYL NEKPSAIF++K            N    L E              
Sbjct: 57  QW-PGKPFQFLGYLTNEKPSAIFRLK------------NTIQTLSE-------------- 89

Query: 121 ANANHNISQLIIGISIEPRNDATAKLEQWKAEQXXXXXXXXXXXMVLSKNSVQILTAGAL 180
            N N      ++GIS+EP  + T                           +V    +  +
Sbjct: 90  -NENCVGITAMLGISVEPLTNFT------------------------ETPAVSTSASNVI 124

Query: 181 AKQYPVVTQELAAKIVQHAYNYLTGFVDAQ--------GN-------VSIKRFDTWWEKF 225
           AK  P VT  +A KI+ + YN+L  F  +Q        G+       + ++ F  W  KF
Sbjct: 125 AKPLPPVT-SVAQKILTNLYNFLASFATSQLPPNSIGLGDLRPNDTFIPLRVFQDWHAKF 183

Query: 226 RVRLQNDATFLD 237
             +L N+  FLD
Sbjct: 184 LNKLSNNPNFLD 195


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.316    0.133    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,581,760
Number of Sequences: 60738
Number of extensions: 547385
Number of successful extensions: 1176
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1171
Number of HSP's gapped (non-prelim): 2
length of query: 242
length of database: 30,389,216
effective HSP length: 103
effective length of query: 139
effective length of database: 24,133,202
effective search space: 3354515078
effective search space used: 3354515078
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)