ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI2214 good A KOG2330 RNA processing and modification Splicing factor 3b, subunit 2
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI2214 792310 790862 -483
(483 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YMR240c [A] KOG2330 Splicing factor 3b subunit 2 296 6e-80
SPAC22F8.10c [A] KOG2330 Splicing factor 3b subunit 2 183 6e-46
Hs5803155 [A] KOG2330 Splicing factor 3b subunit 2 176 5e-44
7295859 [A] KOG2330 Splicing factor 3b subunit 2 174 3e-43
CE14540 [A] KOG2330 Splicing factor 3b subunit 2 165 1e-40
At4g21660 [A] KOG2330 Splicing factor 3b subunit 2 120 6e-27
ECU11g1490 [A] KOG2330 Splicing factor 3b subunit 2 92 2e-18
>YMR240c [A] KOG2330 Splicing factor 3b subunit 2
Length = 436
Score = 296 bits (757), Expect = 6e-80
Identities = 160/389 (41%), Positives = 240/389 (61%), Gaps = 26/389 (6%)
Query: 37 VSSENGNTSQQVINEPLVIKDESLNKVLQRFKASATGADED--DDSKREQAIVRKERADI 94
V++ G T++ V++ L ++ VLQRF+ ++ D K ++ E+ +
Sbjct: 44 VTNSKGKTNK-VVDAKL---EKEFKDVLQRFQVQENDTPKEITKDEKNNHVVI-VEKNPV 98
Query: 95 DDEDAEGSDSFEDQ-ADEVLQNISERQKRLANKPSIAVLKASVPYPELIEWFDCDAQWPY 153
+ D ED +D + +++S R++R KPS++ LK+ VPYP++IEW+DCDA++P
Sbjct: 99 MNRKHTAEDELEDTPSDGIEEHLSARKRRKTEKPSLSQLKSQVPYPQIIEWYDCDARYPG 158
Query: 154 FNAAIKTTHNTVGIPRHWQMKRGYLSGRSMMERKPFQLPEIIRQTDIETMRETVP-SGNE 212
A+IK T N + +P HWQ K+ YLSGRS++ ++PF+LP+II++T+IE MR T+P SG +
Sbjct: 159 LLASIKCTKNVIPVPSHWQSKKEYLSGRSLLGKRPFELPDIIKKTNIEQMRSTLPQSGLD 218
Query: 213 KQAEK-LKDITRAKVQPKLGVLDLDYKRLYEAFFTLGKNWKPE-LLSFGDLYYENRSLDS 270
Q EK LK+ +RA+VQPK+G LDLDYK+L++ FF +G NWKP+ LL FGD+YYENR+L
Sbjct: 219 GQDEKSLKEASRARVQPKMGALDLDYKKLHDVFFKIGANWKPDHLLCFGDVYYENRNLFE 278
Query: 271 ELEWKQIKTRYRPGYLSETLREALNLTEGMLPPWCHEMNRLGLPKSYPGMKVCGINWEIS 330
E WK++ RPG +S+ LR +NL EG LPPWC +M +GLP YP +K+ G+NW+I+
Sbjct: 279 ETNWKRMVDHKRPGRISQELRAIMNLPEGQLPPWCMKMKDIGLPTGYPDLKIAGLNWDIT 338
Query: 331 NLTGTQYG-YYPEDTLRIPKQ--NLFGAMFSFE------ELEDQDAHIDPDPLNESISD- 380
NL G YG P R KQ N FGA+ SFE ED A+ + ++ I D
Sbjct: 339 NLKGDVYGKIIPNHHSRSKKQGRNYFGALISFETPEFENSKEDTQANAENGRQDDKIDDE 398
Query: 381 -----DEVSSDANEAEATKTESETHENNN 404
D D +E + + + E +E +
Sbjct: 399 VEHKLDHFQEDISEVTSAEEKLERNEEES 427
>SPAC22F8.10c [A] KOG2330 Splicing factor 3b subunit 2
Length = 601
Score = 183 bits (464), Expect = 6e-46
Identities = 130/383 (33%), Positives = 190/383 (48%), Gaps = 55/383 (14%)
Query: 50 NEPLVIKDESLNKVLQRFKASATGADEDDDSKREQAIVRKERADIDDEDAEGSDSFEDQA 109
N+PL+ E V RFKA D +D K + E +EDA + Q
Sbjct: 121 NDPLI---EQFKDVFNRFKADGQEKDFEDTDKGQIMYSDDEILSEGEEDA-----LQKQQ 172
Query: 110 DEVLQNISERQKRLANKPSIAVLKASVPYPELIEWFDCDAQWPYFNAAIKTTHNTVGIPR 169
+E L S+++ R + ++A LK +++EW+D + P F +K NTV +PR
Sbjct: 173 EEKL---SKKKLRKLKRMTVAQLKMLSEKADVVEWWDVSSLDPLFLTHLKAYPNTVPVPR 229
Query: 170 HWQMKRGYLSGRSMMERKPFQLPEIIRQTDIETMRETVPSGNEKQAE-KLKDITRAKVQP 228
HW KR YLSG+ +ER+ F+LP IR T I MR V +E +A+ L+ R +VQP
Sbjct: 230 HWNQKRDYLSGQRGIERQLFELPSYIRATGIVQMRNAV---HENEADMPLRQKMRERVQP 286
Query: 229 KLGVLDLDYKRLYEAFFTLGKNWKPELLSFGDLYYENRSLDSELEWKQIKTRYRPGYLSE 288
K+G LD+DY++L++AFF KP L FG+ Y+E + L+++++ K RPG +SE
Sbjct: 287 KMGKLDIDYQKLHDAFFRY--QTKPVLTGFGECYFEGKELEADVKEK------RPGDISE 338
Query: 289 TLREALNLTEGMLPPWCHEMNRLGLPKSYPGMKVCGINWEISNLTGTQYGYYPEDTLRIP 348
LREAL + G PPW M R G P SYP +K+ G+N I TG Q+G++P + P
Sbjct: 339 ELREALGIAPGAPPPWLFAMQRYGPPPSYPDLKIPGVNCPIP--TGAQWGFHPGGWGKPP 396
Query: 349 ----KQNLFGAMFS------------------FEELEDQDAHIDPDPLNESISDDEVSSD 386
+ L+G +F + ELE+ + E S++E S D
Sbjct: 397 VDQFNRPLYGDVFGNVKPRIHAGTGSPVSTQHWGELEEFE--------EEESSEEEESED 448
Query: 387 ANEAEATKTESETHENNNSGLVP 409
TE ET E S P
Sbjct: 449 VEYPTEEITERETIEEYQSASEP 471
>Hs5803155 [A] KOG2330 Splicing factor 3b subunit 2
Length = 872
Score = 176 bits (447), Expect = 5e-44
Identities = 115/336 (34%), Positives = 180/336 (53%), Gaps = 35/336 (10%)
Query: 96 DEDAEGSDSFEDQADEVLQNISERQKRLANKPSIAVLKASVPYPELIEWFDCDAQWPYFN 155
D D + SD +++ E +S+++ R N+ ++A LK V P+++E D AQ P
Sbjct: 407 DSDDDSSDDEQEKKPEA-PKLSKKKLRRMNRFTVAELKQLVARPDVVEMHDVTAQDPKLL 465
Query: 156 AAIKTTHNTVGIPRHWQMKRGYLSGRSMMERKPFQLPEIIRQTDIETMRETVPSGNEKQA 215
+K T N+V +PRHW KR YL G+ +E+ PF+LP+ I++T I+ MRE + E++
Sbjct: 466 VHLKATRNSVPVPRHWCFKRKYLQGKRGIEKPPFELPDFIKRTGIQEMREALQEKEEQKT 525
Query: 216 EKLKDITRAKVQPKLGVLDLDYKRLYEAFFTLGKNW--KPELLSFGDLYYENRSLDSELE 273
K K R KV+PK+G +D+DY++L++AFF W KP+L GDLYYE + ++ L+
Sbjct: 526 MKSK--MREKVRPKMGKIDIDYQKLHDAFF----KWQTKPKLTIHGDLYYEGKEFETRLK 579
Query: 274 WKQIKTRYRPGYLSETLREALNLTEG-----MLPPWCHEMNRLGLPKSYPGMKVCGINWE 328
K +PG LS+ LR +L + G + PPW M R G P SYP +K+ G+N
Sbjct: 580 EK------KPGDLSDELRISLGMPVGPNAHKVPPPWLIAMQRYGPPPSYPNLKIPGLNSP 633
Query: 329 ISNLTGTQYGYY-------PEDTLRIPKQ-NLFGAMFSFEELEDQDAHIDPDPLNESISD 380
I +GY+ P D P ++FG + + + ++ ID P E
Sbjct: 634 IPE--SCSFGYHAGGWGKPPVDETGKPLYGDVFGTNAAEFQTKTEEEEIDRTPWGELEPS 691
Query: 381 DEVSSDANEAEATKTESETHENNNSGLV-PIDGGSV 415
DE SS+ E E ES+ + + +G + P D G +
Sbjct: 692 DEESSEEEEEE----ESDEDKPDETGFITPADSGLI 723
>7295859 [A] KOG2330 Splicing factor 3b subunit 2
Length = 734
Score = 174 bits (441), Expect = 3e-43
Identities = 126/396 (31%), Positives = 203/396 (50%), Gaps = 45/396 (11%)
Query: 43 NTSQQVINEPLVIKD-----ESLNKVLQRFKAS--ATGADEDDDSKREQAIVRKERA--- 92
N + + + E + I D +V + FK ++D S ++ R ++A
Sbjct: 203 NVTIEYVPEKITIADLAPMYRQFYRVFEIFKLENKPKPVEKDKSSHDSESHPRDKKATDK 262
Query: 93 DIDDEDAEGSDSFEDQADEVLQNISERQKRLANKPSIAVLKASVPYPELIEWFDCDAQWP 152
++DED +G D E + D+ + +S+R+ + + S+A LK V P+++E D A+ P
Sbjct: 263 QLEDEDDDGDDDEERKEDK--EKLSKRKLKKLTRLSVAELKQLVSRPDVVEMHDVTARDP 320
Query: 153 YFNAAIKTTHNTVGIPRHWQMKRGYLSGRSMMERKPFQLPEIIRQTDIETMRETVPSGNE 212
+K NTV +PRHW KR YL G+ +E+ PF LP I++T I MRE++
Sbjct: 321 KLLVQLKAYRNTVQVPRHWCFKRKYLQGKRGIEKPPFDLPAFIKKTGIMEMRESLQ--ER 378
Query: 213 KQAEKLKDITRAKVQPKLGVLDLDYKRLYEAFFTLGKNW--KPELLSFGDLYYENRSLDS 270
+ A+ LK R +V+PK+G +D+DY++L++AFF W KP + GDLYYE + ++
Sbjct: 379 EDAKTLKAKMRERVRPKMGKIDIDYQKLHDAFF----KWQTKPRMTIHGDLYYEGKEFET 434
Query: 271 ELEWKQIKTRYRPGYLSETLREALNLTEG-----MLPPWCHEMNRLGLPKSYPGMKVCGI 325
L+ K +PG LSE LR AL + G + PPW R G P SYP +K+ G+
Sbjct: 435 RLKEK------KPGDLSEELRIALGMPVGPNSHKIPPPWLIAQQRYGPPPSYPNLKIPGL 488
Query: 326 NWEISNLTGTQYGYYPEDTLRIPKQN----LFGAMFSFEELE----DQDAHIDPDPLNES 377
N I + GT +GY+ + P L+G +F L+ +A I+ + E
Sbjct: 489 NAPIPD--GTSFGYHAGGWGKPPVDENGKPLYGDVFGTNILDLDNGVDEADIERNQWGEL 546
Query: 378 ISDDEVSSDANEAEATKTESETHENNNSGLV-PIDG 412
S+ E SS+ E + E + + +GLV P++G
Sbjct: 547 ESESEESSEEEEEDG---EDLGDQQDETGLVTPVEG 579
>CE14540 [A] KOG2330 Splicing factor 3b subunit 2
Length = 602
Score = 165 bits (418), Expect = 1e-40
Identities = 117/369 (31%), Positives = 188/369 (50%), Gaps = 40/369 (10%)
Query: 67 FKASATGADEDDDSKREQAIVRKERAD----IDDEDAEGSDSFEDQADE-VLQNISERQK 121
F A D +D + + E+ D + + + E++A E + +S R+
Sbjct: 93 FDAFKISTDSEDGFSYGHHVSKGEKKDDLGKVGQAERIIQEEMEERAKENTEEKLSRRKL 152
Query: 122 RLANKPSIAVLKASVPYPELIEWFDCDAQWPYFNAAIKTTHNTVGIPRHWQMKRGYLSGR 181
R++ +PSIA LK + +++EW D ++ PY A+K+ N+V +PRHW KR YL+G+
Sbjct: 153 RISLQPSIAKLKETTLRADVVEWADVTSRDPYLLVAMKSYRNSVPVPRHWNAKRKYLAGK 212
Query: 182 SMMERKPFQLPEIIRQTDIETMRETVPSGNEKQAEKLKDITRAKVQPKLGVLDLDYKRLY 241
ER PF+LP+ I++T I+ MRE + E Q+ LK R + +PKLG +D+DY++L+
Sbjct: 213 RGFERPPFELPDFIKRTGIQDMREALLEKEESQS--LKSKMRERARPKLGKIDIDYQKLH 270
Query: 242 EAFFTLGKNW--KPELLSFGDLYYENRSLDSELEWKQIKTRYRPGYLSETLREALNLTEG 299
+AFF W KP + G+LYYE + +++ + K +PG +S+ LR AL + G
Sbjct: 271 DAFF----KWQTKPAMTKMGELYYEGKEMEAMMRDK------KPGEMSDELRIALGMPIG 320
Query: 300 -----MLPPWCHEMNRLGLPKSYPGMKVCGINWEISNLTGTQYGYY-------PEDTLRI 347
PPW M R G P S+P +K+ G+N I G +GY+ P D
Sbjct: 321 SNAFKFPPPWLIAMQRYGPPPSFPHIKIPGLNAPIPE--GCAFGYHAGGWGKPPVDEYGH 378
Query: 348 PKQ-NLFGAMFSFEELEDQDAHIDPDPLNESISDDEVSSDANEAEATKTESETHENNNSG 406
P ++FG E ED ++ I+ E SD+ SSD E+E + + + G
Sbjct: 379 PLYGDVFGLAAPAFEPED-ESQIERRYWGEIGSDE--SSDEEESEEEEHADDDDADVEGG 435
Query: 407 L---VPIDG 412
P++G
Sbjct: 436 FQTPAPVEG 444
>At4g21660 [A] KOG2330 Splicing factor 3b subunit 2
Length = 669
Score = 120 bits (300), Expect = 6e-27
Identities = 82/256 (32%), Positives = 128/256 (49%), Gaps = 28/256 (10%)
Query: 180 GRSMMERKPFQLPEIIRQTDIETMRET------VPSGNEKQ-AEKLKDITRAKVQPKLGV 232
G+ +E++PF LP+ I T IE +R+ + EK+ +KLK R ++QPK+G
Sbjct: 283 GKRGIEKQPFHLPDFIAATGIEKIRQVKVILYQYKAYIEKEDGKKLKQKQRERMQPKMGK 342
Query: 233 LDLDYKRLYEAFFTLGKNWKPELLSFGDLYYENRSLDSELEWKQIKTRYRPGYLSETLRE 292
+D+DY+ L++AFF KP+L + GDLY+E + + +L +PG+LS L+E
Sbjct: 343 MDIDYQVLHDAFFKY--QTKPKLSALGDLYFEGKEFEVKLR------ETKPGFLSNDLKE 394
Query: 293 ALNLTEGMLPPWCHEMNRLGLPKSYPGMKVCGINWEISNLTGTQYGYY-------PEDTL 345
AL + EG PPW M R G P SYP +K+ G+N I G +G++ P D
Sbjct: 395 ALGMPEGAPPPWLINMQRYGPPPSYPHLKIPGLNAPIP--IGASFGFHAGGWGKPPVDEY 452
Query: 346 RIPKQNLFGAMFSFEELEDQDAHIDPDPLNESISDDEVSSDANEAEATKTESETHENN-N 404
P L+G +F ++ + + +P ++ D E + E E + E E E
Sbjct: 453 GRP---LYGDVFGVQQQDQPNYEEEPIDKSKHWGDLEEEEEEEEEEEEEQEEEMDEEELE 509
Query: 405 SGLVPIDGGSVVNQHY 420
G +D S HY
Sbjct: 510 DGTESVDTLSRYCYHY 525
Score = 63.9 bits (154), Expect = 5e-10
Identities = 43/150 (28%), Positives = 73/150 (48%), Gaps = 13/150 (8%)
Query: 38 SSENGNTSQQVINEPLVIKDE-------SLNKVLQRFKASATGADEDDDSKREQAIVRKE 90
S EN + Q++ E + + E ++ ++F A E+D +K E +E
Sbjct: 65 SKENTDPQPQIVIEYVPEQAEFEDGFNDEFKEIFEKFNFREPLASEEDGTKDES----EE 120
Query: 91 RADIDDEDAEGSDSFEDQADEVLQN--ISERQKRLANKPSIAVLKASVPYPELIEWFDCD 148
+ D+ + SDS +D+ D + IS ++K+L + IA LK P+++E +D
Sbjct: 121 KEDVKKKVNSDSDSDDDEQDNQNKEKGISNKKKKLQRRMKIAELKQVSARPDVVEVWDAT 180
Query: 149 AQWPYFNAAIKTTHNTVGIPRHWQMKRGYL 178
+ P +K+ NTV +PRHW KR YL
Sbjct: 181 SADPKLLVFLKSYRNTVPVPRHWSQKRKYL 210
>ECU11g1490 [A] KOG2330 Splicing factor 3b subunit 2
Length = 333
Score = 92.0 bits (227), Expect = 2e-18
Identities = 74/228 (32%), Positives = 108/228 (46%), Gaps = 26/228 (11%)
Query: 119 RQKRLANKPSIAVLKASVPYPELIEWFDCDAQWPYFNAAIKTTHNTVGIPRHWQMKRGYL 178
++KR A + LK VPYPE+ E D P + +K V +PRHW+
Sbjct: 38 QRKRRALQHRYEELKLVVPYPEIFETEDATCPDPMSHNRMKGCSG-VPVPRHWRS----- 91
Query: 179 SGRSMMER---KP-FQLPEIIRQTDIETMRETVPSGNEKQAE-KLKDITRAKVQPKLGVL 233
S R M KP + P + T I +R + E++A L++ R K+ P++G
Sbjct: 92 SSRRMFPHGYHKPRYVTPRHVIGTGIPELRRMM---REREAGMSLRERIREKLHPRVGGS 148
Query: 234 DLDYKRLYEAFFTLGKNWKPELLSFGDLYYENRSLDSELEWKQIKTRYRPGYLSETLREA 293
+D + LYEAFF+LG +P L +G+ + +D E K PG +S L EA
Sbjct: 149 LVDQRILYEAFFSLGP--RPYLSKYGEFF---EPVDDYFEKKCF-----PGAISADLMEA 198
Query: 294 LNLTEGMLPPWCHEMNRLGLPKSYPGMKVCGINWEISNLTGTQYGYYP 341
L + PPW M + G+P SYP ++ G+N I G YGY P
Sbjct: 199 LGIDSSTPPPWLFNMQKHGMPPSYPDARIPGLNAPIPE--GCSYGYQP 244
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.311 0.131 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,781,504
Number of Sequences: 60738
Number of extensions: 1305149
Number of successful extensions: 6047
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 6014
Number of HSP's gapped (non-prelim): 9
length of query: 483
length of database: 30,389,216
effective HSP length: 110
effective length of query: 373
effective length of database: 23,708,036
effective search space: 8843097428
effective search space used: 8843097428
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)