ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI2229 good C KOG0756 Energy production and conversion Mitochondrial tricarboxylate/dicarboxylate carrier proteins
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI2229 796328 797251 308
(308 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YMR241w [C] KOG0756 Mitochondrial tricarboxylate/dicarboxylate c... 469 e-132
SPBC83.13 [C] KOG0756 Mitochondrial tricarboxylate/dicarboxylate... 291 1e-78
Hs13449279 [C] KOG0754 Mitochondrial oxodicarboxylate carrier pr... 57 3e-08
At1g14140 [C] KOG0753 Mitochondrial fatty acid anion carrier pro... 52 8e-07
YKL120w [C] KOG0755 Mitochondrial oxaloacetate carrier protein 51 2e-06
YJR095w [C] KOG0756 Mitochondrial tricarboxylate/dicarboxylate c... 51 2e-06
Hs22053628 [C] KOG0752 Mitochondrial solute carrier protein 50 4e-06
Hs21389315 [C] KOG0756 Mitochondrial tricarboxylate/dicarboxylat... 50 4e-06
>YMR241w [C] KOG0756 Mitochondrial tricarboxylate/dicarboxylate carrier
proteins
Length = 314
Score = 469 bits (1208), Expect = e-132
Identities = 230/302 (76%), Positives = 250/302 (82%), Gaps = 1/302 (0%)
Query: 8 LKKKPISFSNILLGAGLNMCEVTTLGQPLEVVKTTMAANRQFTFFQAINHIWSRGGVFGF 67
++KKP+SFSNILLGA LN+ EVTTLGQPLEVVKTTMAANR FTF +++ H+WSRGG+ G+
Sbjct: 13 IEKKPVSFSNILLGACLNLSEVTTLGQPLEVVKTTMAANRNFTFLESVKHVWSRGGILGY 72
Query: 68 YQGLIPWAWIEASTKGAVLLFVSAEAEYHFKKLGLNNFXXXXXXXXXXXXXQAYLTMGFC 127
YQGLIPWAWIEASTKGAVLLFVSAEAEY FK LGLNNF QAYLTMGFC
Sbjct: 73 YQGLIPWAWIEASTKGAVLLFVSAEAEYRFKSLGLNNFASGILGGVTGGVTQAYLTMGFC 132
Query: 128 TCMKTVEITXXXXXXXXXXXXXX-XXFKEIYNKEGIRGINKGVNAVAIRQMTNWGSRFGF 186
TCMKTVEIT FK IY KEGIRGINKGVNAVAIRQMTNWGSRFG
Sbjct: 133 TCMKTVEITRHKSASAGGVPQSSWSVFKNIYKKEGIRGINKGVNAVAIRQMTNWGSRFGL 192
Query: 187 SRLVEEGLRKFTGKTNPDDKLTALEKIFASAIGGGLSAWNQPIEVIRVEMQSKTNDPNRP 246
SRLVE+G+RK TGKTN DDKL EKI ASA+GGGLSAWNQPIEVIRVEMQSK DPNRP
Sbjct: 193 SRLVEDGIRKITGKTNKDDKLNPFEKIGASALGGGLSAWNQPIEVIRVEMQSKKEDPNRP 252
Query: 247 KDLTVGKAFRYIYQSNGVKGLYRGVTPRIGLGIWQTVFMVGCGDIAREFVGKLTGETPVA 306
K+LTVGK F+YIYQSNG+KGLYRGVTPRIGLGIWQTVFMVG GD+A+EFV ++TGETPVA
Sbjct: 253 KNLTVGKTFKYIYQSNGLKGLYRGVTPRIGLGIWQTVFMVGFGDMAKEFVARMTGETPVA 312
Query: 307 KH 308
KH
Sbjct: 313 KH 314
>SPBC83.13 [C] KOG0756 Mitochondrial tricarboxylate/dicarboxylate carrier
proteins
Length = 293
Score = 291 bits (744), Expect = 1e-78
Identities = 154/293 (52%), Positives = 188/293 (63%), Gaps = 3/293 (1%)
Query: 7 ELKKKPISFSNILLGAGLNMCEVTTLGQPLEVVKTTMAANRQFTFFQAINHIWSRGGVFG 66
+++KK S SN+LLGAGLN+ E ++LGQPLEV K MAANR T QAI I SRGG+ G
Sbjct: 3 KIEKKQASASNLLLGAGLNLFETSSLGQPLEVTKVQMAANRTQTMAQAIKAIMSRGGILG 62
Query: 67 FYQGLIPWAWIEASTKGAVLLFVSAEAEYHFKKLGLNNFXXXXXXXXXXXXXQAYLTMGF 126
FYQGLIPWAWIEASTKGAVLLF SAE EY ++LGL QAY TMGF
Sbjct: 63 FYQGLIPWAWIEASTKGAVLLFTSAELEYWGRRLGLGATSAGAIAGMGGGVAQAYATMGF 122
Query: 127 CTCMKTVEIT-XXXXXXXXXXXXXXXXFKEIYNKEGIRGINKGVNAVAIRQMTNWGSRFG 185
CTCMKT E+T F ++Y ++GIRGIN+GVNAVA+RQMTNWGSR
Sbjct: 123 CTCMKTAEVTRAKQAATGQEVKGTFRVFADLYKEKGIRGINRGVNAVALRQMTNWGSRLA 182
Query: 186 FSRLVEEGLRKFTGKTNPDDKLTALEKIFASAIGGGLSAWNQPIEVIRVEMQSKTNDPNR 245
SR +E+ +R FTG+T + LT ++ AS G LS WNQP+EV RVEMQS T
Sbjct: 183 LSRFLEKPIRYFTGRTEA-EPLTTGQRFVASVSAGALSCWNQPLEVARVEMQSLTKGIQH 241
Query: 246 PKDLTVGKAFRYIYQSNGVKGLYRGVTPRIGLGIWQTVFMVGCGDIAREFVGK 298
+ + IY++NG+KGLYRG PR+GLG +QT MV D R ++ K
Sbjct: 242 SSP-GIMQTIMSIYKNNGIKGLYRGAVPRMGLGAYQTFVMVFLADCVRGYLAK 293
>Hs13449279 [C] KOG0754 Mitochondrial oxodicarboxylate carrier protein
Length = 299
Score = 57.0 bits (136), Expect = 3e-08
Identities = 68/286 (23%), Positives = 112/286 (38%), Gaps = 23/286 (8%)
Query: 1 MSSQPVELKKKPISFSNILLGAGLNMCEVTTLGQPLEVVKTTMAANRQFT-------FFQ 53
MS++P E+ + I+ G + E+ + PL+VVKT R T
Sbjct: 1 MSAKP-EVSLVREASRQIVAGGSAGLVEICLM-HPLDVVKTRFQIQRCATDPNSYKSLVD 58
Query: 54 AINHIWSRGGVFGFYQGLIPWAWIEASTKGAVLLFVSAEAEYHFKKLGLNNFXXXXXXXX 113
+ I+ G+FGFY+G++P E + K AV F + + + L+
Sbjct: 59 SFRMIFQMEGLFGFYKGILPPILAE-TPKRAVKFFTFEQYKKLLGYVSLSPALTFAIAGL 117
Query: 114 XXXXXQAYLTMGFCTCMKTVEITXXXXXXXXXXXXXXXXF-KEIYNKEG--IRGINKGVN 170
+A + F + V++ + ++I KEG ++G+NKG+
Sbjct: 118 GSGLTEAIVVNPF----EVVKVGLQANRNTFAEQPSTVGYARQIIKKEGWGLQGLNKGLT 173
Query: 171 AVAIRQMTNWGSRFGFSRLVEEGLRKFTGKTNPDDKLTALEKIFASAIGGGL-SAWNQPI 229
A R FGF V+ + N D L K + G + S N P
Sbjct: 174 ATLGRHGVFNMVYFGFYYNVKNMI-----PVNKDPILEFWRKFGIGLLSGTIASVINIPF 228
Query: 230 EVIRVEMQSKTNDPNRPKDLTVGKAFRYIYQSNGVKGLYRGVTPRI 275
+V + +Q P K T K +YQ G+ LY+G+ P+I
Sbjct: 229 DVAKSRIQGPQPVPGEIKYRTCFKTMATVYQEEGILALYKGLLPKI 274
>At1g14140 [C] KOG0753 Mitochondrial fatty acid anion carrier
protein/Uncoupling protein
Length = 305
Score = 52.4 bits (124), Expect = 8e-07
Identities = 43/147 (29%), Positives = 68/147 (46%), Gaps = 8/147 (5%)
Query: 155 EIYNKEGIRGINKGVNAVAIRQMTNWGSRF-GFSRLVEEGLRKFTGKTNPDDKLTALEKI 213
EI KEG+ G+ KG++ IR + R G+ L +R +TN + L K
Sbjct: 62 EIARKEGVIGLYKGLSPAIIRHLFYTPIRIIGYENLKGLIVRS---ETNNSESLPLATKA 118
Query: 214 FASAIGGGLS-AWNQPIEVIRVEMQSK---TNDPNRPKDLTVGKAFRYIYQSNGVKGLYR 269
G ++ P ++++V MQ+ + +P+ +AF I QS GVKGL++
Sbjct: 119 LVGGFSGVIAQVVASPADLVKVRMQADGRLVSQGLKPRYSGPIEAFTKILQSEGVKGLWK 178
Query: 270 GVTPRIGLGIWQTVFMVGCGDIAREFV 296
GV P I + + C D A+ FV
Sbjct: 179 GVLPNIQRAFLVNMGELACYDHAKHFV 205
>YKL120w [C] KOG0755 Mitochondrial oxaloacetate carrier protein
Length = 324
Score = 51.2 bits (121), Expect = 2e-06
Identities = 37/131 (28%), Positives = 63/131 (47%), Gaps = 17/131 (12%)
Query: 156 IYNKEGIRGINKGVNAVAIRQMTNWGSRFGFSRLVEEGLRK-FTGKTNPDDKLTALEKIF 214
I+ EGI+G+ KG+NA I Q+ GSR GF + L + F P + +F
Sbjct: 74 IFKNEGIKGLQKGLNAAYIYQIGLNGSRLGFYEPIRSSLNQLFFPDQEPHKVQSVGVNVF 133
Query: 215 ASAIGGGLSA-WNQPIEVIRVEMQSKTNDPNRPKDLTVGKAFRY---------IYQSNGV 264
+ A G + A P+ +++ +QS + + + +G+ Y I+++ GV
Sbjct: 134 SGAASGIIGAVIGSPLFLVKTRLQSYS------EFIKIGEQTHYTGVWNGLVTIFKTEGV 187
Query: 265 KGLYRGVTPRI 275
KGL+RG+ I
Sbjct: 188 KGLFRGIDAAI 198
>YJR095w [C] KOG0756 Mitochondrial tricarboxylate/dicarboxylate carrier
proteins
Length = 322
Score = 50.8 bits (120), Expect = 2e-06
Identities = 35/126 (27%), Positives = 59/126 (46%), Gaps = 13/126 (10%)
Query: 156 IYNKEGIRGINKGVNAVAIRQMTNWGSRFGFSRLVEEGLRKFTGKTNPDDKLTALEKIFA 215
I +EG+ + +GV+ A RQ TN G+ F V L++F + D L + E
Sbjct: 165 IVKEEGVSALYRGVSLTAARQATNQGANF----TVYSKLKEFLQNYHQMDVLPSWETSCI 220
Query: 216 SAIGGGLSAW-NQPIEVIRVEMQ-----SKTNDPNRPKDLTVGKAFRYIYQSNGVKGLYR 269
I G + + N P++ I+ +Q S K +T+G + + G + LY+
Sbjct: 221 GLISGAIGPFSNAPLDTIKTRLQKDKSISLEKQSGMKKIITIG---AQLLKEEGFRALYK 277
Query: 270 GVTPRI 275
G+TPR+
Sbjct: 278 GITPRV 283
>Hs22053628 [C] KOG0752 Mitochondrial solute carrier protein
Length = 332
Score = 50.1 bits (118), Expect = 4e-06
Identities = 68/278 (24%), Positives = 105/278 (37%), Gaps = 38/278 (13%)
Query: 20 LGAGLNMCEVTTLGQPLEVVKTTMAAN----RQFTFFQAINHIWSRGGVFGFYQG----- 70
L G+ C T PL+ VK + A+ + F A+ + + G G Y+G
Sbjct: 41 LAGGIAGCCAKTTVAPLDRVKVLLQAHNHHYKHLGVFSALRAVPQKEGFLGLYKGNGAMM 100
Query: 71 --LIPWAWIEASTKGAVLLFVSAEAEYHFKKLGLNNFXXXXXXXXXXXXXQAYLTMGFCT 128
+ P+ I+ F++ E H+K L A +T CT
Sbjct: 101 IRIFPYGAIQ---------FMAFE---HYKTLITTKLGISGHVHRLMAGSMAGMTAVICT 148
Query: 129 C---MKTVEITXXXXXXXXXXXXXXXXFKEIYNKEG-IRGINKGVNAVAIRQMTNWG-SR 183
M V + FK IY KEG G +G+ + G S
Sbjct: 149 YPLDMVRVRLAFQVKGEHSYTGIIHA-FKTIYAKEGGFFGFYRGLMPTILGMAPYAGVSF 207
Query: 184 FGFSRLVEEGLRK---FTGKTNPDDKLTALEKIFASAIGGGLSAW-----NQPIEVIRVE 235
F F L GL G+ + D+ + K + + GG++ + P +V R
Sbjct: 208 FTFGTLKSVGLSHAPTLLGRPSSDNPNVLVLKTHVNLLCGGVAGAIAQTISYPFDVTRRR 267
Query: 236 MQSKTNDPNRPKDLTVGKAFRYIYQSNGV-KGLYRGVT 272
MQ T P K LT+ +Y+Y +G+ KGLYRG++
Sbjct: 268 MQLGTVLPEFEKCLTMRDTMKYVYGHHGIRKGLYRGLS 305
>Hs21389315 [C] KOG0756 Mitochondrial tricarboxylate/dicarboxylate carrier
proteins
Length = 311
Score = 50.1 bits (118), Expect = 4e-06
Identities = 35/124 (28%), Positives = 56/124 (44%), Gaps = 9/124 (7%)
Query: 154 KEIYNKEGIRGINKGVNAVAIRQMTNWGSRFGFSRLVEEGLRKFTGKTNPDDKLTALEKI 213
+EI ++G++G +G+ A ++Q +N RF V LR + NP+ + L
Sbjct: 169 REIVREQGLKGTYQGLTATVLKQGSNQAIRF----FVMTSLRNWYRGDNPNKPMNPLITG 224
Query: 214 FASAIGGGLSAW-NQPIEVIRVEMQSKTNDPNRPKDLTVGKAFRYIYQSNGVKGLYRGVT 272
AI G S + N P++VI+ MQ R I + G+K Y+G
Sbjct: 225 VFGAIAGAASVFGNTPLDVIKTRMQGLEAHKYR----NTWDCGLQILKKEGLKAFYKGTV 280
Query: 273 PRIG 276
PR+G
Sbjct: 281 PRLG 284
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.321 0.138 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,220,574
Number of Sequences: 60738
Number of extensions: 617834
Number of successful extensions: 1662
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1637
Number of HSP's gapped (non-prelim): 16
length of query: 308
length of database: 30,389,216
effective HSP length: 106
effective length of query: 202
effective length of database: 23,950,988
effective search space: 4838099576
effective search space used: 4838099576
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)