ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI2241 good A KOG2217 RNA processing and modification U4/U6.U5 snRNP associated protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI2241 801263 799593 -557
(557 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR308c [A] KOG2217 U4/U6.U5 snRNP associated protein 142 2e-33
SPAC167.03c [A] KOG2217 U4/U6.U5 snRNP associated protein 70 6e-12
CE27372 [A] KOG2217 U4/U6.U5 snRNP associated protein 54 5e-07
Hs10863889 [A] KOG2217 U4/U6.U5 snRNP associated protein 50 9e-06
>YOR308c [A] KOG2217 U4/U6.U5 snRNP associated protein
Length = 587
Score = 142 bits (357), Expect = 2e-33
Identities = 151/602 (25%), Positives = 265/602 (43%), Gaps = 71/602 (11%)
Query: 1 MGKITSISLEETNRIRASLGLKTIPRPTETSTESKEGAS-DTNRKVRRSSQLSSRPSEKD 59
M K ++S+EETN IR LG+K IP E +T+ KE S + ++R S L P +++
Sbjct: 1 MNKTENLSIEETNEIREKLGMKPIPVFQEKNTDHKESLSIEETNELRASLGLKLIPPQQN 60
Query: 60 CASGKINGSAQDRDKKIGQLKQRI--YESKEAVDQRAT-----TDKESIDDWLSKIGK-P 111
S N KI +L+++I ++ K R T+ WL + P
Sbjct: 61 FNSSPPN---VHNTSKIDELREKITKFQKKANAPLRMAHLLEETNVNDDSSWLENMDAIP 117
Query: 112 ITH--KESSA---ENATVHDD-ITHHHIPVSDELKSIAEQKAVIFTLKETSVTXXXXXXX 165
+H K SS + AT D+ I H++ VS +++++ +K I TLKE+S+
Sbjct: 118 SSHESKRSSTLPRKGATKEDENIDLHNVQVSYNIEALSPKKDTILTLKESSI---FDDTD 174
Query: 166 XXXXXXXXKLVHDKQDAKNLKLRQLNKDRKLNK----ISIFDYSDEANDTNSRSDIPLH- 220
K + D + L+LRQ+NKDR+ K +S D +E + S H
Sbjct: 175 STEVLENVKAAEENADREKLRLRQMNKDRRQKKKILNVSSLDIEEE-EEGEKHSITTTHL 233
Query: 221 ----DSLVNDKPLPVHQSLPK-RVKIESDSDDEQPYQD---YAPVXXXXXXXXXXXXXXX 272
+ V P + P +VK+ DS + +D + P+
Sbjct: 234 IIGAEQGVMKAPNTISAKPPTGKVKVNFDSANNMSDEDGGDFKPLKIKKRKIKDPRSTKA 293
Query: 273 XXID-TARMVKVDLLEDDEDFSDRPIQAVVKVKQKR--SNEEITGIKN-SLELEKL--EG 326
T +M V L+++DE S + V + R SN E+ G ++ + E+EK E
Sbjct: 294 RKSKITDKMEIVKLVDEDESLSWMEEEQPVTIINPRTSSNNELKGPEDLAREIEKARDEE 353
Query: 327 QQRSKGIHMLNNSDKGILLDETDEFLDSLKANVLDREPVNDDLPIKIDLKPVQKDKTGTI 386
++R++ I + I++DE FL++L ++ +R + + + + + D T
Sbjct: 354 KRRTESILKMREISNSIVVDEKVTFLNTLDTSLSERSATENKVKVHGEGEKNIGDVTNGH 413
Query: 387 PATLVTDNDITQ---------------PDFSGGVAGMLNFLKERNIIKQDDRPNMGSDSK 431
+N +T+ P+F G+A L +L+++++ G
Sbjct: 414 TKEGSGNNTLTEAVNNEPNYEGDAENAPNFFSGLASTLGYLRKKSVF------TTGDVDL 467
Query: 432 KHGTDVRKEFEILNGIVNSLKTVDEKLDSNDVNFSEDELRKIKAAQAEEMALKIQRIQEE 491
K G DV + + N K + +++D+L ++ + + + +
Sbjct: 468 KPGKDVNNSESLRRDVRN-------KEHTGTGTYTKDKLHGLEQFTSSDSSNA--NTHSK 518
Query: 492 KLRDYNPTVELAYQDSEGNELKTKEAYKQLSQAWHGTKSSKKTLERQRKKMADRKKQLER 551
+ Y+P ++L Y+D +GN L TKEAYK+LSQ +HGTKS+KK + + ++ RK E
Sbjct: 519 RQDHYDPDIKLVYRDEKGNRLTTKEAYKKLSQKFHGTKSNKKKRAKMKSRIEARKNTPEN 578
Query: 552 DS 553
S
Sbjct: 579 GS 580
>SPAC167.03c [A] KOG2217 U4/U6.U5 snRNP associated protein
Length = 649
Score = 70.5 bits (171), Expect = 6e-12
Identities = 137/657 (20%), Positives = 249/657 (37%), Gaps = 129/657 (19%)
Query: 2 GKITSISLEETNRIRASLGLKTIPRPTETSTESKEGASDTNRKVRRSSQLSS--RPSEKD 59
G SIS+EETNRIR SLGLK + E + AS + V + Q + E++
Sbjct: 6 GASESISIEETNRIRISLGLKPLDISEEKPQKELSDASVKSSYVDQEQQAYENWKKQEQE 65
Query: 60 CASGKINGSAQDRDKKIGQLKQRIYESK-EAVDQRATTDKESIDD-----WLSKIGKPIT 113
+ K + + +K+ Q +R ++ + + + + + ID W+ K+
Sbjct: 66 EINRKKEEELKSKFEKLRQKNERRRRTQGKTLAETLAEEDDQIDANDTRAWILKMRNSSL 125
Query: 114 HKESSAEN----------ATVHDDITHHHIPVSDELKSIA-------EQKAVIFTLKETS 156
+K + N +T+H ++ S E IA + ++ TLK+
Sbjct: 126 NKNGNGLNFEAKNDAPRRSTLHSQNAGPNVNSSLEGMKIAHGFQDLNKNDGLVLTLKDAD 185
Query: 157 VTXXXXXXXXXXXXXXXKLVHDKQDAKNLKLRQLNKDRKLNKISIFDYSDEAND--TNSR 214
+ HD + + R+ +RK + Y DE ND S
Sbjct: 186 ILDEDN--------------HDLLENVEMVQRKTKNERKEDN-PYKPYEDE-NDFLQQSE 229
Query: 215 SDIPLHDSLVNDKPLPVHQSLPKRVKIESD-SDDEQPYQDYAPVXXXXXXXXXXXXXXXX 273
+D P D+ P SL IE SD+++ +
Sbjct: 230 ADQPSEDTFTTIGP---QNSLTVNSTIEKHKSDNKKTFGTLVSFVEPTITGSEQSDYRQI 286
Query: 274 XIDTA-----------RMVKVDLLEDDE---------------DFSDRPIQAVVKVK--- 304
I + R+V++D ++E D S A+ K +
Sbjct: 287 KIKKSKKKKSKSDRRKRLVELDAENENENDPSDFVLPNGQSNSDLSVEQDSAIFKEQKKM 346
Query: 305 --QKRSNEEIT-------GIKNSLELEKLEGQQRSKGI------HMLNNSDK-------- 341
QKR E T ++ S+ +++ Q+R+K + L N+++
Sbjct: 347 KIQKRMRELETQSFADDDDLQQSIAMQRRLAQKRAKILKPEDVAEQLQNAEEVTDMTDSD 406
Query: 342 ---GILLDETDEFLDSLKAN-------------VLDREPVNDDLPIK-----IDLKPVQK 380
G++ D+T F++S+K D + +ND I + + +
Sbjct: 407 TASGLIFDDTRAFVNSIKETENREALGSINEQAFEDSKDLNDTNSITGSSPTEESNALVE 466
Query: 381 DKTGTIPATLVT-----DNDITQPDFSGGVAGMLNFLKERNIIKQDD--RPNMGSDSKKH 433
D + I ATL +N +P S V +L+ L+ + +IK D + + + + +
Sbjct: 467 DTSVDISATLEEANTQQENAEDEPLVSDNVGAVLSLLRNKGVIKVSDEAKEKIQKEEEYN 526
Query: 434 GTDVRKE-FEILNGIVNSLKTVDEKLDSNDVNFSEDELRKIKAAQAEEMALKIQRIQEEK 492
RK+ + K ++L ++ E + + E KI I+ E+
Sbjct: 527 KWFARKQQARVELEEQRRKKKEQDRLSGKFEKMTQKEREQYAKKENERWDKKIAEIELEQ 586
Query: 493 LRDYNPTVELAYQDSEGNELKTKEAYKQ-LSQAWHGTKSSKKTLERQRKKMADRKKQ 548
DY P V++ Y D G EL KEAYK LS +HG S K E++ +++ +++++
Sbjct: 587 FHDYKPQVDIKYVDEFGVELGPKEAYKYLLSHQFHGKGSGKAKTEKRLRRIVEKERE 643
>CE27372 [A] KOG2217 U4/U6.U5 snRNP associated protein
Length = 829
Score = 54.3 bits (129), Expect = 5e-07
Identities = 36/158 (22%), Positives = 70/158 (43%), Gaps = 23/158 (14%)
Query: 420 QDDRPNMGSDSKKHGTDVRKEFEILNGIVNSLKTVDEK---LDSNDVNFSEDELRKIKAA 476
+++ +M D + + +E ++ G+ LK +K + N + S L +K
Sbjct: 636 EEEDEDMEEDKDDYAPALGQEVDVSKGVGRMLKLAGQKGYLQNPNAKSHSGPSLDHLKNK 695
Query: 477 QAEEM-----------ALKIQRIQEE---------KLRDYNPTVELAYQDSEGNELKTKE 516
+ + A K R+ + + DY P V ++Y D +G E+ K+
Sbjct: 696 TTQRIDGQRFDPDDRNAKKADRLNSQHRGPVMPFAEKHDYKPDVNISYVDRKGREMDAKD 755
Query: 517 AYKQLSQAWHGTKSSKKTLERQRKKMADRKKQLERDSY 554
AY++LS +HG KK LE++ + ++ L+ +SY
Sbjct: 756 AYRELSYKFHGRNPGKKQLEKRANRKDKEERMLKTNSY 793
>Hs10863889 [A] KOG2217 U4/U6.U5 snRNP associated protein
Length = 800
Score = 50.1 bits (118), Expect = 9e-06
Identities = 29/72 (40%), Positives = 41/72 (56%), Gaps = 4/72 (5%)
Query: 489 QEEKLRD-YNPTVELAYQDSEGNELKTKEAYKQLSQAWHGTKSSKKTLERQRKKMADR-- 545
Q+ K +D Y P V++ Y D G +L KEA++QLS +HG S K ER+ KK+ +
Sbjct: 696 QDFKEKDGYKPDVKIEYVDETGRKLTPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEAL 755
Query: 546 -KKQLERDSYLG 556
KK D+ LG
Sbjct: 756 LKKMSSSDTPLG 767
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.308 0.128 0.342
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,461,041
Number of Sequences: 60738
Number of extensions: 1330901
Number of successful extensions: 5507
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 5495
Number of HSP's gapped (non-prelim): 8
length of query: 557
length of database: 30,389,216
effective HSP length: 112
effective length of query: 445
effective length of database: 23,586,560
effective search space: 10496019200
effective search space used: 10496019200
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)