ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI2248 good S KOG4453 Function unknown Predicted ER membrane protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI2248 804683 803835 -283
(283 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR311c [S] KOG4453 Predicted ER membrane protein 398 e-111
SPBC3D6.05 [S] KOG4453 Predicted ER membrane protein 160 1e-39
At5g04490 [S] KOG4453 Predicted ER membrane protein 52 1e-06
>YOR311c [S] KOG4453 Predicted ER membrane protein
Length = 290
Score = 398 bits (1022), Expect = e-111
Identities = 193/273 (70%), Positives = 225/273 (81%), Gaps = 6/273 (2%)
Query: 14 ELKQRLSTPNGVNTKKQKETVVLGKSLS----DVHLPLTEIHLKSHEWFGDFITKHEIPR 69
E KQRLS+ + + K T+ + +S D H+P+TEIHLKSHEWFGDFITKHEIPR
Sbjct: 19 EAKQRLSSKS--HQVSAKVTIPAKEEISSSDDDAHVPVTEIHLKSHEWFGDFITKHEIPR 76
Query: 70 KLFHSSIGFITLYLYTQNVDYKKVKFPLIVGFTVVFILDFIRLRSPFFNKMYCRCVGALM 129
K+FHSSIGFITLYLYTQ ++YK V +PLI F ++FILD IRL PFFN +YCR VGALM
Sbjct: 77 KVFHSSIGFITLYLYTQGINYKNVLWPLIYAFIILFILDLIRLNWPFFNMLYCRTVGALM 136
Query: 130 RKKEIHTYNGVLWYLLGLTFAFSFFSKDIALISLFLLSWSDTAASTFGRKYGHLTPKLAR 189
RKKEIHTYNGVLWY+LGL F+F+FFSKD+ LISLFLLSWSDTAA+T GRKYGHLTPK+AR
Sbjct: 137 RKKEIHTYNGVLWYILGLIFSFNFFSKDVTLISLFLLSWSDTAAATIGRKYGHLTPKVAR 196
Query: 190 NKSLAGSIAAFSVGLITCYSFYGFFVPHYNWVNKPGEISWTEETSKXXXXXXXXXGGFVA 249
NKSLAGSIAAF+VG+ITC+ FYG+FVP Y++VNKPGEI W+ ETS+ GG VA
Sbjct: 197 NKSLAGSIAAFTVGVITCWVFYGYFVPAYSYVNKPGEIQWSPETSRLSLNMLSLLGGVVA 256
Query: 250 ALSEGIDLFNWDDNFTIPVLSAIFMTAVIRIFK 282
ALSEGIDLFNWDDNFTIPVLS++FM AVI+ FK
Sbjct: 257 ALSEGIDLFNWDDNFTIPVLSSLFMNAVIKTFK 289
>SPBC3D6.05 [S] KOG4453 Predicted ER membrane protein
Length = 218
Score = 160 bits (406), Expect = 1e-39
Identities = 89/217 (41%), Positives = 129/217 (59%), Gaps = 13/217 (5%)
Query: 64 KHEIPRKLFHSSIGFITLYLYTQNVDYKKVKF-PLI-VGFTVVFILDFIRLRSPFFNKMY 121
KHEIPRK H+SIGF L L Q Y + P+I +GF F D IR P F+++Y
Sbjct: 13 KHEIPRKALHTSIGFFALLL--QGCGYHAAQIIPVIEIGFIPAFTGDVIRFNWPAFSRLY 70
Query: 122 CRCVGALMRKKEIHTYNGVLWYLLGLTFAFSFFSKDIALISLFLLSWSDTAASTFGRKYG 181
R +G LMR+ E + +NGV++Y++G+ F ++IA++S+ LLSW DT AST GRK+G
Sbjct: 71 NRVIGPLMRESEKNAWNGVIFYMIGVWIVLKVFPEEIAVMSVLLLSWCDTTASTVGRKWG 130
Query: 182 HLTPKLARNKSLAGSIAAFSVGLITCYSFYGFFVPHYNWVNKPGEISWTEETSKXXXXXX 241
TPK+A+NKSLAGS+ AF G+ CY ++G F + G S + S+
Sbjct: 131 KYTPKIAKNKSLAGSLGAFVCGVFCCYVYWGLF--------RTGPDSLAAQ-SRIPFPWL 181
Query: 242 XXXGGFVAALSEGIDLFNWDDNFTIPVLSAIFMTAVI 278
GF+ A +E +D++ DDN IPV+SA + ++
Sbjct: 182 CLINGFIGAFAEAMDVWGLDDNLVIPVVSACLLYLIM 218
>At5g04490 [S] KOG4453 Predicted ER membrane protein
Length = 304
Score = 51.6 bits (122), Expect = 1e-06
Identities = 50/172 (29%), Positives = 75/172 (43%), Gaps = 22/172 (12%)
Query: 62 ITKHEIPRKLFHSSIGFITLYLY-----TQNVDYKKVKFPLIVGFTVVFILDFIRLRSPF 116
+ + + RKL H G + + + + Y PL+ G +V I S
Sbjct: 96 VIQQSLSRKLVHILSGLLFVLAWPIFSGSTEARYFAAFVPLVNGLRLV-----INGLSIS 150
Query: 117 FNKMYCRCVGALMRKKEIHTYNGVLWYLLGLTFAFSFFSKD--IALISLFLLSWSDTAAS 174
N M + V R +E+ G L+Y+L L F+ FF ++ I +ISL ++ D A
Sbjct: 151 PNSMLIKSVTREGRAEEL--LKGPLFYVLALLFSAVFFWRESPIGMISLAMMCGGDGIAD 208
Query: 175 TFGRKYGHLTPKLARNKSLAGSIAAF------SVGLITCYSFYGFFVPHYNW 220
GRK+G KS AGSI+ F S+ L+ YS G+ H NW
Sbjct: 209 IMGRKFGSTKIPYNPRKSWAGSISMFIFGFFISIALLYYYSSLGYL--HMNW 258
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.325 0.140 0.434
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,712,522
Number of Sequences: 60738
Number of extensions: 676914
Number of successful extensions: 1941
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1937
Number of HSP's gapped (non-prelim): 3
length of query: 283
length of database: 30,389,216
effective HSP length: 105
effective length of query: 178
effective length of database: 24,011,726
effective search space: 4274087228
effective search space used: 4274087228
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)