ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI2306 good I KOG0872 Lipid transport and metabolism Sterol C5 desaturase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI2306 823595 824653 353
(353 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR056w [I] KOG0872 Sterol C5 desaturase 463 e-130
SPAC1687.16c [I] KOG0872 Sterol C5 desaturase 318 8e-87
Hs5902070 [I] KOG0872 Sterol C5 desaturase 274 2e-73
Hs20558831 [I] KOG0872 Sterol C5 desaturase 274 2e-73
At3g02590 [I] KOG0872 Sterol C5 desaturase 101 2e-21
At3g02580 [I] KOG0872 Sterol C5 desaturase 98 2e-20
7292910 [I] KOG0873 C-4 sterol methyl oxidase 68 2e-11
At1g14290 [I] KOG0874 Sphingolipid hydroxylase 65 2e-10
At1g69640 [I] KOG0874 Sphingolipid hydroxylase 62 1e-09
YDR297w [I] KOG0874 Sphingolipid hydroxylase 60 4e-09
CE03386 [I] KOG0873 C-4 sterol methyl oxidase 60 4e-09
7292879 [I] KOG0873 C-4 sterol methyl oxidase 59 8e-09
SPBC887.15c [I] KOG0874 Sphingolipid hydroxylase 59 1e-08
CE03385 [I] KOG0873 C-4 sterol methyl oxidase 57 3e-08
Hs5803157 [I] KOG0873 C-4 sterol methyl oxidase 57 5e-08
At4g22750_1 [I] KOG0873 C-4 sterol methyl oxidase 51 2e-06
>YLR056w [I] KOG0872 Sterol C5 desaturase
Length = 365
Score = 463 bits (1192), Expect = e-130
Identities = 213/357 (59%), Positives = 275/357 (76%), Gaps = 12/357 (3%)
Query: 1 MDLLLEVCDTYLLDYVYGKVFPVNMLPSSVTNSVGQFLDLNTGNLNGT----------AA 50
MDL+LEV D Y+LD +Y KV P + L +++ + L LN+G N T A
Sbjct: 1 MDLVLEVADHYVLDDLYAKVLPAS-LAANIPVKWQKLLGLNSGFSNSTILQETLNSKNAV 59
Query: 51 KPAGDVYGYEPFLLEFTDYTFQSIFPRYNLFRETLTMFVLMTIFGWALYFSVATMSYLFV 110
K YG PFL + + +F S+ PR ++ RE L+++V++TIFG LY A++SY+FV
Sbjct: 60 KECRRFYGQVPFLFDMSTTSFASLLPRSSILREFLSLWVIVTIFGLLLYLFTASLSYVFV 119
Query: 111 FDKAVFNHPRYLKNQMSLEMKQAFSAIPVMVLLTIPWFLFELNGYSKLYLDIDYKSWAGI 170
FDK++FNHPRYLKNQM++E+K A SAIP M +LT+PWF+ ELNG+SKLY+ IDY++ G+
Sbjct: 120 FDKSIFNHPRYLKNQMAMEIKLAVSAIPWMSMLTVPWFVMELNGHSKLYMKIDYENH-GV 178
Query: 171 RQLAFEVISFIMFTDCLIYLVHRWLHWPRVYRALHKPHHKWLVTTPYASHAFHPVDGYMQ 230
R+L E +FI FTDC +YL HRWLHWPRVYRALHKPHHKWLV TP+ASH+FHPVDG++Q
Sbjct: 179 RKLIIEYFTFIFFTDCGVYLAHRWLHWPRVYRALHKPHHKWLVCTPFASHSFHPVDGFLQ 238
Query: 231 SVPYHLYPMIFPLQKISYLILFTFVNFWTVMIHDGEYLANDPVINGAACHTVHHLYFNYN 290
S+ YH+YP+I PL K+SYLILFTFVNFWTVMIHDG+YL+N+P +NG ACHTVHHLYFNYN
Sbjct: 239 SISYHIYPLILPLHKVSYLILFTFVNFWTVMIHDGQYLSNNPAVNGTACHTVHHLYFNYN 298
Query: 291 YGQFTTLWDRLGGSYREPDQELFNKSLKKSKQTWEIQVEKMEAIKQEVEGDCDDRVY 347
YGQFTTLWDRLGGSYR PD LF+ L+ +K+TW+ QV+++E +EVEGD +DR+Y
Sbjct: 299 YGQFTTLWDRLGGSYRRPDDSLFDPKLRDAKETWDAQVKEVEHFIKEVEGDDNDRIY 355
>SPAC1687.16c [I] KOG0872 Sterol C5 desaturase
Length = 300
Score = 318 bits (815), Expect = 8e-87
Identities = 152/299 (50%), Positives = 212/299 (70%), Gaps = 13/299 (4%)
Query: 62 FLLEFTD-YTFQSIF------PRYNLFRETLTMFVLMTIFGWALYFSVATMSYLFVFDKA 114
+LL + D Y SI+ R N+ R+++++F L G LY + A++SY FVFDK+
Sbjct: 3 YLLNYADQYALDSIYNAVYPLARDNIVRQSISLFFLTWFGGMFLYLTFASLSYQFVFDKS 62
Query: 115 VFNHPRYLKNQMSLEMKQAFSAIPVMVLLTIPWFLFELNGYSKLYLDI-DYKSWAGIRQL 173
+ +HP++LKNQ+ +E+ A +P M LLT+PWFL EL+GYS LY +I DY G++
Sbjct: 63 LMDHPKFLKNQVFMEVLTALQNLPGMALLTVPWFLAELHGYSYLYDNISDY----GLKYF 118
Query: 174 AFEVISFIMFTDCLIYLVHRWLHWPRVYRALHKPHHKWLVTTPYASHAFHPVDGYMQSVP 233
+ F+MF+D IY HR+LH VY LHK HHKW++ TPYASHAF DG++QS+P
Sbjct: 119 LCSLPLFVMFSDFGIYWAHRFLHHRYVYPRLHKLHHKWIICTPYASHAFKSADGFLQSLP 178
Query: 234 YHLYPMIFPLQKISYLILFTFVNFWTVMIHDGEYLANDPVINGAACHTVHHLYFNYNYGQ 293
YHL+P FPL K++YL LFTFVNFW++MIHDG+Y++N+P+INGAA H HH+YFNYNYGQ
Sbjct: 179 YHLFPFFFPLHKLTYLALFTFVNFWSIMIHDGKYISNNPIINGAAHHNGHHIYFNYNYGQ 238
Query: 294 FTTLWDRLGGSYREPDQELFNKSLKKSKQTWEIQVEKMEAIKQEVEGDCDDRVYVTTKA 352
FTTL+DRLG S+R PD+ F+K L++++ +++ + EAI+ EVEGD DDR Y+ A
Sbjct: 239 FTTLFDRLGNSFRAPDEAWFDKDLRQNEDVLRVELMEYEAIRNEVEGD-DDREYIANSA 296
>Hs5902070 [I] KOG0872 Sterol C5 desaturase
Length = 299
Score = 274 bits (700), Expect = 2e-73
Identities = 120/246 (48%), Positives = 173/246 (69%), Gaps = 4/246 (1%)
Query: 66 FTDYTFQSIFPRYNLFRETLTMFVLMTIFGWALYFSVATMSYLFVFDKAVFNHPRYLKNQ 125
FT Y + + +P ++FR+ +++ ++ + + LYF AT+SY FVFD A+ HP++LKNQ
Sbjct: 13 FTPYVYPATWPEDDIFRQAISLLIVTNVGAYILYFFCATLSYYFVFDHALMKHPQFLKNQ 72
Query: 126 MSLEMKQAFSAIPVMVLLTIPWFLFELNGYSKLYLDIDYKSWAGIRQLAFEVISFIMFTD 185
+ E+K A+P + +LT+ FL E+ GYSKL+ D+ + G+ +L +ISF+ FTD
Sbjct: 73 VRREIKFTVQALPWISILTVALFLLEIRGYSKLHDDLGEFPY-GLFELVVSIISFLFFTD 131
Query: 186 CLIYLVHRWLHWPRVYRALHKPHHKWLVTTPYASHAFHPVDGYMQSVPYHLYPMIFPLQK 245
IY +HR LH VY+ LHKPHH W + TP+ASHAFHP+DG++QS+PYH+YP IFPL K
Sbjct: 132 MFIYWIHRGLHHRLVYKRLHKPHHIWKIPTPFASHAFHPIDGFLQSLPYHIYPFIFPLHK 191
Query: 246 ISYLILFTFVNFWTVMIHDGEYLAN---DPVINGAACHTVHHLYFNYNYGQFTTLWDRLG 302
+ YL L+ VN WT+ IHDG++ P ING+A HT HH++F+YNYGQ+ TLWDR+G
Sbjct: 192 VVYLSLYILVNIWTISIHDGDFRVPQILQPFINGSAHHTDHHMFFDYNYGQYFTLWDRIG 251
Query: 303 GSYREP 308
GS++ P
Sbjct: 252 GSFKNP 257
>Hs20558831 [I] KOG0872 Sterol C5 desaturase
Length = 299
Score = 274 bits (700), Expect = 2e-73
Identities = 120/246 (48%), Positives = 173/246 (69%), Gaps = 4/246 (1%)
Query: 66 FTDYTFQSIFPRYNLFRETLTMFVLMTIFGWALYFSVATMSYLFVFDKAVFNHPRYLKNQ 125
FT Y + + +P ++FR+ +++ ++ + + LYF AT+SY FVFD A+ HP++LKNQ
Sbjct: 13 FTPYVYPATWPEDDIFRQAISLLIVTNVGAYILYFFCATLSYYFVFDHALMKHPQFLKNQ 72
Query: 126 MSLEMKQAFSAIPVMVLLTIPWFLFELNGYSKLYLDIDYKSWAGIRQLAFEVISFIMFTD 185
+ E+K A+P + +LT+ FL E+ GYSKL+ D+ + G+ +L +ISF+ FTD
Sbjct: 73 VRREIKFTVQALPWISILTVALFLLEIRGYSKLHDDLGEFPY-GLFELVVSIISFLFFTD 131
Query: 186 CLIYLVHRWLHWPRVYRALHKPHHKWLVTTPYASHAFHPVDGYMQSVPYHLYPMIFPLQK 245
IY +HR LH VY+ LHKPHH W + TP+ASHAFHP+DG++QS+PYH+YP IFPL K
Sbjct: 132 MFIYWIHRGLHHRLVYKRLHKPHHIWKIPTPFASHAFHPIDGFLQSLPYHIYPFIFPLHK 191
Query: 246 ISYLILFTFVNFWTVMIHDGEYLAN---DPVINGAACHTVHHLYFNYNYGQFTTLWDRLG 302
+ YL L+ VN WT+ IHDG++ P ING+A HT HH++F+YNYGQ+ TLWDR+G
Sbjct: 192 VVYLSLYILVNIWTISIHDGDFRVPQILQPFINGSAHHTDHHMFFDYNYGQYFTLWDRIG 251
Query: 303 GSYREP 308
GS++ P
Sbjct: 252 GSFKNP 257
>At3g02590 [I] KOG0872 Sterol C5 desaturase
Length = 279
Score = 101 bits (251), Expect = 2e-21
Identities = 64/192 (33%), Positives = 104/192 (53%), Gaps = 12/192 (6%)
Query: 123 KNQMSLEMKQAFSAIPVMVLL-TIPWFLFELNGYSKLYLDIDYKSWAGIRQLAFEVIS-F 180
+ M L++ A A+P LL + ++ E +G++K Y +D+ +W L F I+ +
Sbjct: 82 RKAMLLQIYVAMKAMPWYTLLPAVSEYMIE-HGWTKCYSTLDHFNWF----LCFLYIALY 136
Query: 181 IMFTDCLIYLVHRWLHWPR-VYRALHKPHH---KWLVTTPYASHAFHPVDGYMQSVPYHL 236
++ + +IY VH+ LH + +Y+ LH HH K +P+A AFHP+DG +Q++P+ +
Sbjct: 137 LVLVEFMIYWVHKELHDIKFLYKHLHATHHMYNKQNTLSPFAGLAFHPLDGILQAIPHVI 196
Query: 237 YPMIFPLQKISYLILFTFVNFWTVMIHDGEYLANDPVINGAACHTVHHLYFNYNYGQFTT 296
I P+ I++L L WT IHD + N I GA HT+HH + +NYG +T
Sbjct: 197 ALFIVPIHLITHLSLLFLEGIWTASIHDCIH-GNIWPIMGAGYHTIHHTTYKHNYGHYTI 255
Query: 297 LWDRLGGSYREP 308
D + GS P
Sbjct: 256 WMDWMFGSLMVP 267
>At3g02580 [I] KOG0872 Sterol C5 desaturase
Length = 281
Score = 98.2 bits (243), Expect = 2e-20
Identities = 70/204 (34%), Positives = 109/204 (53%), Gaps = 14/204 (6%)
Query: 126 MSLEMKQAFSAIPVMVLL-TIPWFLFELNGYSKLYLDIDYKSWAGIRQLAFEVIS-FIMF 183
M L+M A A+P LL T+ + E G++K + I W L F I+ +++F
Sbjct: 84 MRLQMFVAMKAMPWYTLLPTVSESMIE-RGWTKCFASIGEFGWI----LYFVYIAIYLVF 138
Query: 184 TDCLIYLVHRWLHWPR-VYRALHKPHH---KWLVTTPYASHAFHPVDGYMQSVPYHLYPM 239
+ IY +HR LH + +Y+ LH HH K +P+A AFHPVDG +Q+VP+ +
Sbjct: 139 VEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPVDGILQAVPHVIALF 198
Query: 240 IFPLQKISYLILFTFVNFWTVMIHDGEYLANDPVINGAACHTVHHLYFNYNYGQFTTLWD 299
I P+ +++ L WT IHD + PV+ GA HT+HH + +NYG +T D
Sbjct: 199 IVPIHFTTHIGLLFMEAIWTANIHDCIHGNIWPVM-GAGYHTIHHTTYKHNYGHYTIWMD 257
Query: 300 RLGGSYREP--DQELFNKSLKKSK 321
+ GS R+P +++ S KK++
Sbjct: 258 WMFGSLRDPLLEEDDNKDSFKKAE 281
>7292910 [I] KOG0873 C-4 sterol methyl oxidase
Length = 278
Score = 67.8 bits (164), Expect = 2e-11
Identities = 56/223 (25%), Positives = 100/223 (44%), Gaps = 20/223 (8%)
Query: 115 VFNHPRYLKNQM-------SLEMKQAFSAIPVMVL-LTIPWFLFELNGYSKLYLDI---D 163
+ N PR+L+ + + + + A+ V++ LT+ FL Y +Y D
Sbjct: 58 ITNRPRFLRKYKIQPGQNEPVNLAKLWHAVKVVLFNLTVVNFLVSWVVYEFVYKSENSQD 117
Query: 164 YKSWAGIRQLAFEVISFIMFTDCLIYLVHRWLHWPRVYRALHKPHHKWLVTTPYASHAFH 223
+ ++ +++ F++ + + Y HR LH VY+ +HK HH+W + H
Sbjct: 118 IRVLPTFKRSLRDLVVFVVLEEIMFYYAHRLLHHRSVYKYVHKKHHEWTAPIAAITLYAH 177
Query: 224 PVDGYMQSVPYHLYPMIFPLQKISYLILFTFVNFWTVMIH---DGEYLANDPVINGAACH 280
PV + V +L P+ + + + +V F +I+ D + H
Sbjct: 178 PV----EHVLANLLPVATSIAILGTHVALAYVIFALAIINSMSDHTGYSFPWSAGSVRFH 233
Query: 281 TVHHLYFNYNYGQFTTLWDRLGGSYREP-DQELFNKSLKKSKQ 322
HH FNYNYG L D+L G+YR P +Q+ ++ +KK K+
Sbjct: 234 DYHHAKFNYNYGVLGFL-DKLHGTYRAPAEQKAPSQRIKKIKK 275
>At1g14290 [I] KOG0874 Sphingolipid hydroxylase
Length = 258
Score = 65.1 bits (157), Expect = 2e-10
Identities = 41/142 (28%), Positives = 70/142 (48%), Gaps = 9/142 (6%)
Query: 173 LAFEVISFIMFTDCLIYLVHRWLHWPR-VYRALHKPHHKWLVTTPYASHAFHPVDGYMQS 231
LA + I ++ D Y +HR++H + +Y+ +H HH+ +V Y + HP++G +
Sbjct: 93 LARQFIIAMLVIDTWQYFIHRYMHLNKFLYKHIHSQHHRLIVPYSYGALYNHPLEGLLLD 152
Query: 232 VPYHLYPMIFP-LQKISYLILFTFVNFWTVMIHDGEYLANDP----VINGAACHTVHHLY 286
+F + + + F+F TV H G +L +P N +A H VHH
Sbjct: 153 TIGGALSFLFSGMSPRTAIFFFSFATIKTVDDHCGLWLPGNPFHIFFSNNSAYHDVHHQL 212
Query: 287 F--NYNYGQ-FTTLWDRLGGSY 305
+ YN+ Q F +WDR+ G+Y
Sbjct: 213 YGTKYNFSQPFFVMWDRILGTY 234
>At1g69640 [I] KOG0874 Sphingolipid hydroxylase
Length = 258
Score = 62.4 bits (150), Expect = 1e-09
Identities = 39/142 (27%), Positives = 73/142 (50%), Gaps = 9/142 (6%)
Query: 173 LAFEVISFIMFTDCLIYLVHRWLHWPR-VYRALHKPHHKWLVTTPYASHAFHPVDG-YMQ 230
LA + ++ ++ D Y +HR++H + +Y+ +H HH+ +V Y + HPV+G +
Sbjct: 93 LARQFVTAMIVLDTWQYFMHRYMHQNKFLYKHIHSQHHRLIVPYAYGALYNHPVEGLLLD 152
Query: 231 SVPYHLYPMIFPLQKISYLILFTFVNFWTVMIHDGEYLAND----PVINGAACHTVHHLY 286
++ L ++ + + + F+F TV H G +L + N +A H +HH
Sbjct: 153 TIGGALSFLVSGMSPRTSIFFFSFATIKTVDDHCGLWLPGNLFHMVFKNNSAYHDIHHQL 212
Query: 287 F--NYNYGQ-FTTLWDRLGGSY 305
+ YN+ Q F +WDR+ G+Y
Sbjct: 213 YGTKYNFSQPFFVMWDRILGTY 234
>YDR297w [I] KOG0874 Sphingolipid hydroxylase
Length = 349
Score = 60.5 bits (145), Expect = 4e-09
Identities = 50/176 (28%), Positives = 82/176 (46%), Gaps = 14/176 (7%)
Query: 174 AFEVISFIMFTDCLIYLVHRWLHWPR-VYRALHKPHHKWLVTTPYASHAFHPVDGY-MQS 231
A ++ + +F D Y +HR +H + +Y+ H HH+ V Y + +PV+G+ + +
Sbjct: 158 ALKIFAGFLFVDTWQYFLHRLMHMNKTLYKWFHSVHHELYVPYAYGALFNNPVEGFLLDT 217
Query: 232 VPYHLYPMIFPLQKISYLILFTFVNFWTVMIHDGEYLANDP----VINGAACHTVHHLYF 287
+ + + L +ILFTF TV H G L DP N A H +HH F
Sbjct: 218 LGTGIAMTLTHLTHREQIILFTFATMKTVDDHCGYALPLDPFQWLFPNNAVYHDIHHQQF 277
Query: 288 NY--NYGQ-FTTLWDRLGGS----YREPDQELFNKSLKKSKQ-TWEIQVEKMEAIK 335
N+ Q F T WD L + + E ++ ++ K K+ E ++EK E +K
Sbjct: 278 GIKTNFAQPFFTFWDNLFQTNFKGFEEYQKKQRRVTIDKYKEFLQERELEKKEKLK 333
>CE03386 [I] KOG0873 C-4 sterol methyl oxidase
Length = 286
Score = 60.5 bits (145), Expect = 4e-09
Identities = 31/105 (29%), Positives = 51/105 (48%), Gaps = 4/105 (3%)
Query: 189 YLVHRWLHWPRVYRALHKPHHKWLVTTPYASHAFHPVDGYMQSV-PYHLYPMIFPLQKIS 247
Y HR H P++Y+ +HK HH+W +S HP++ + ++ P +L ++F +S
Sbjct: 126 YYTHRLFHHPKLYKHIHKKHHEWNAPVSISSIYAHPLEHAISNLSPIYLGAVLFRCHVVS 185
Query: 248 YLILFTFVNFWTVMIHDGEYLANDPVINGAACHTVHHLYFNYNYG 292
+ I ++ T H G + P + A H HH FN YG
Sbjct: 186 HYIFTSYAILTTTFHHSGYHF---PFMLSAEHHDFHHKVFNECYG 227
>7292879 [I] KOG0873 C-4 sterol methyl oxidase
Length = 406
Score = 59.3 bits (142), Expect = 8e-09
Identities = 50/186 (26%), Positives = 75/186 (39%), Gaps = 14/186 (7%)
Query: 120 RYLKNQMSLEMKQAFSAIPVMVLLTIPWFLFELNGYSKLYLDIDYKSWAGIRQLAFEVIS 179
R LK + Q F IP L + L E+ G S + D+ W + FE+
Sbjct: 202 RLLKVIWCVVCNQIFVGIP---LAYASYRLMEIRGLSDIR-DLPTFHW-----VCFELTV 252
Query: 180 FIMFTDCLIYLVHRWLHWPRVYRALHKPHHKWLVTTPYASHAFHPVDG-YMQSVPYHLYP 238
I+ + Y HR LH +Y+ +HK HH+W + HP++ + +P L
Sbjct: 253 CILMEELGFYYSHRLLHHKHIYKYIHKQHHEWTAPISVTAIYCHPIEHIFSNLLPPFLGV 312
Query: 239 MIFPLQKISYLILFTFVNFWTVMIHDGEYLANDPVINGAACHTVHHLYFNYNYGQFTTLW 298
+ + + F T+ H G +L P H HHL FN +G L
Sbjct: 313 FLMGSHVATAWLWFALAILSTLNAHSGYHL---PFFPSPEAHDFHHLKFNNCFGVLGVL- 368
Query: 299 DRLGGS 304
DRL G+
Sbjct: 369 DRLHGT 374
>SPBC887.15c [I] KOG0874 Sphingolipid hydroxylase
Length = 293
Score = 58.5 bits (140), Expect = 1e-08
Identities = 49/167 (29%), Positives = 73/167 (43%), Gaps = 19/167 (11%)
Query: 174 AFEVISFIMFTDCLIYLVHRWLHW-PRVYRALHKPHHKWLVTTPYASHAF--HPVDGYMQ 230
AF+ D Y HR+LH+ ++Y +H HH+ V PYA A HP +G +
Sbjct: 131 AFQYFFAFFIIDSWQYFWHRYLHYNKKLYNMIHAHHHRLQV--PYAMGALYNHPFEGLIL 188
Query: 231 SV----PYHLYPMIFPLQKISYLILFTFVNFWTVMIHDGEYLANDPV----INGAACHTV 282
+L + P Q + I FT TV H G DP+ N A H +
Sbjct: 189 DTFGAGVAYLAAGLSPQQAV---IFFTLSTLKTVDDHCGYVFPYDPLQMFFANNARYHDL 245
Query: 283 HHLYFNY--NYGQ-FTTLWDRLGGSYREPDQELFNKSLKKSKQTWEI 326
HH + + N+ Q F T WD + G+Y P E + +K+K ++
Sbjct: 246 HHQPYGFQKNFSQPFFTFWDHVLGTYMPPKSETPYEKKQKAKNAKKV 292
>CE03385 [I] KOG0873 C-4 sterol methyl oxidase
Length = 269
Score = 57.4 bits (137), Expect = 3e-08
Identities = 44/171 (25%), Positives = 75/171 (43%), Gaps = 13/171 (7%)
Query: 172 QLAFEVISFIMFTDCLIYLVHRWLHWPRVYRALHKPHHKWLVTTPYASHAFHPVDGYMQS 231
Q+ +++ I+ + Y HR H P++Y+ +HK HH+W S HP++ + +
Sbjct: 108 QIFRDIVISILCEEIGFYYSHRLFHHPKIYKYIHKKHHEWTAPVSITSIYCHPLEHAISN 167
Query: 232 V-PYHLYPMIFPLQKISYLILFTFVNFWTVMIHDGEYLANDPVINGAACHTVHHLYFNYN 290
+ P L P I I+ I + T H G + P + H HH FN
Sbjct: 168 LSPVLLGPTICGSHVITLWIWASIAILSTTCSHSGYHF---PFMLSPEPHDYHHKVFNEC 224
Query: 291 YGQFTTLWDRLGGSYREPDQELFNKSL--KKSKQTWEIQVEKMEAIKQEVE 339
+G T L D + G+ F KS+ K++ +W ++ K +Q+ E
Sbjct: 225 FG--TGLLDWIHGT-----DSTFRKSIEGKRNYMSWSMKPIKQIHPEQQKE 268
>Hs5803157 [I] KOG0873 C-4 sterol methyl oxidase
Length = 293
Score = 56.6 bits (135), Expect = 5e-08
Identities = 60/266 (22%), Positives = 101/266 (37%), Gaps = 27/266 (10%)
Query: 78 YNLFRETLTMFVLMT----IFGWALYFSVATMSYLFVFD------KAVFNHPRYLKNQMS 127
+N T F + T I ALYF +LF F K + P +NQ
Sbjct: 37 WNYMLNNYTKFQIATWGSLIVHEALYFLFCLPGFLFQFIPYMKKYKIQKDKPETWENQWK 96
Query: 128 LEMKQAFSAIPVMVLLTIPWFLFELNGYSKLYLDIDYKSWAGIRQLAF---EVISFIMFT 184
F+ + + L + F Y +I Y W + + F +
Sbjct: 97 CFKVLLFNHFCIQLPLICGTYYFTE------YFNIPY-DWERMPRWYFLLARCFGCAVIE 149
Query: 185 DCLIYLVHRWLHWPRVYRALHKPHHKWLVTTPYASHAFHPVDGYMQSVPYHLYPMIFPLQ 244
D Y +HR LH R+Y+ +HK HH++ + HP++ + + + ++
Sbjct: 150 DTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDH 209
Query: 245 KISYLILFTFVNFWTVMIHDGEYLANDPV-----INGAACHTVHHLYFNYNYGQFTTLWD 299
I T T+ +H G + +P+ G+ H HH+ F NY T WD
Sbjct: 210 VILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMNFIGNYASTFTWWD 269
Query: 300 RLGGSYREPDQELFNKSLKKSKQTWE 325
R+ G+ + +N+ KK ++ E
Sbjct: 270 RIFGT--DSQYNAYNEKRKKFEKKTE 293
>At4g22750_1 [I] KOG0873 C-4 sterol methyl oxidase
Length = 524
Score = 51.2 bits (121), Expect = 2e-06
Identities = 52/258 (20%), Positives = 98/258 (37%), Gaps = 28/258 (10%)
Query: 85 LTMFVLMTIFGWALYFSVATMSYLFVFDKAVFNHPRYLKNQMSLEMKQAFSAIPVMVLLT 144
L +F++ ++ L F ++ S +F++ + +KN S K + + +L+
Sbjct: 43 LFLFLIFSLVPLPLVFIESSQSTSDLFNR--YKIQPKVKNSFSSMFKCYKDVMKMFILVV 100
Query: 145 IPWFLFELNGYSKLYLDIDY---KSWAGIR---------QLAFEVISFIMFTDCLIYLVH 192
P +L Y + +D + K IR ++ +++ + + D Y VH
Sbjct: 101 GP---LQLVSYPSIQVDFVFRVLKQMIEIRSGLPLPSCMEIVAQLVVYFLVEDYTNYWVH 157
Query: 193 RWLHWPRVYRALHKPHHKWLVTTPYASHAFHPVDGYMQSVPYHLYPMIFPLQKISYLILF 252
R+ H Y H HH++ YA+ H + + +P L P I P I++ +
Sbjct: 158 RFFHCKWGYEKFHHIHHEYTAPIGYAAPYAHWAEVLLLGIPTFLGPAIAPGHMITFWLWI 217
Query: 253 TFVNFWTVMIHDG--------EYLANDPVINGAACHTVHHLYFNYNYGQFTTLWDRLGGS 304
+ H G +Y+ P GA H HH + F +++
Sbjct: 218 ALRQIEAIETHSGYDFPWSLTKYI---PFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYI 274
Query: 305 YREPDQELFNKSLKKSKQ 322
Y F K L + K+
Sbjct: 275 YGTDKGYRFQKKLLQQKK 292
Score = 51.2 bits (121), Expect = 2e-06
Identities = 28/134 (20%), Positives = 55/134 (40%), Gaps = 5/134 (3%)
Query: 170 IRQLAFEVISFIMFTDCLIYLVHRWLHWPRVYRALHKPHHKWLVTTPYASHAFHPVDGYM 229
+ ++ +++ + + D Y +HRW+H Y +H+ HH++ YAS H + +
Sbjct: 340 LMEIVAQLVVYFLIEDYTNYWIHRWMHCKWGYEKIHRIHHEYTSPIGYASPYAHWAEILI 399
Query: 230 QSVPYHLYPMIFPLQKISYLILFTFVNFWTVMIHDG-----EYLANDPVINGAACHTVHH 284
+P L P I P +++ + + F + H G P G H HH
Sbjct: 400 LGIPTFLGPAIAPGHIMTFWLWISLRQFEAIETHSGYDFPWSVTKLIPFYGGPEYHDYHH 459
Query: 285 LYFNYNYGQFTTLW 298
+ F +++
Sbjct: 460 YVGGQSQSNFASVF 473
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.327 0.141 0.455
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,010,774
Number of Sequences: 60738
Number of extensions: 962082
Number of successful extensions: 2976
Number of sequences better than 1.0e-05: 16
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 2945
Number of HSP's gapped (non-prelim): 19
length of query: 353
length of database: 30,389,216
effective HSP length: 107
effective length of query: 246
effective length of database: 23,890,250
effective search space: 5877001500
effective search space used: 5877001500
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)