ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactVI2313   suspect: LH   G     KOG2204  Carbohydrate transport and metabolism                          Mannosyl-oligosaccharide alpha-1,2-mannosidase and related glycosyl hydrolases

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactVI2313 826574  828682 703  
         (703 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YLR057w [G] KOG2204 Mannosyl-oligosaccharide alpha-12-mannosidas...   200   7e-51

>YLR057w [G] KOG2204 Mannosyl-oligosaccharide alpha-12-mannosidase and
           related glycosyl hydrolases
          Length = 849

 Score =  200 bits (508), Expect = 7e-51
 Identities = 201/773 (26%), Positives = 328/773 (42%), Gaps = 151/773 (19%)

Query: 4   RLLLHTWRKCRSILLLLITASVLFYYTFENEIEHLNSFAYTDSLPLIQNQDSHNAPVAGS 63
           RL+   +R+ RS+LLL IT S+LFYYTF+NEI+ LNS+A  DSLP I N + HN   +  
Sbjct: 5   RLVYSLFRRVRSVLLLFITISLLFYYTFQNEIDILNSYALNDSLPSINNYE-HNTEGSSK 63

Query: 64  ----------ADKITHDE----ISKELTDKSSLSEKNKYFPILLENSDEALKYRSE---- 105
                     +D+I  D+    ++ +L+D ++L EKNKYFP+LL+ S   +         
Sbjct: 64  LDPPDLSSTGSDRIATDKENGNVAVDLSDPATLREKNKYFPLLLKGSSHQIGSNLPISSL 123

Query: 106 -NYDSKEPFHPFHPFYEKKTVIPYKQISKQYPQLNRVSSIHSNALIKDELLYHWTARINR 164
             Y  K P   F       T I    + K  P +     +     IKD  +  W      
Sbjct: 124 LTYKEKYPVL-FEYSSPSLTSISQNDVHKIQPAMQLPPDVDMIKQIKDIFMKSWNQEQLL 182

Query: 165 ITDHAYLDLQTNISIL-STDSLLQRNESLLTVTAV--LNKFD--------DPMDQRVWSS 213
           +  +   +    I ++ S D+L    E+ L   +V  +  FD        + +D    ++
Sbjct: 183 LKSNLRRESTWPIDLIDSLDTLYLCGETKLFQDSVNIIEDFDFRVPPLAMEVIDIPDITT 242

Query: 214 EILGSLLAVIGNRKEKNPELLQLITIIGEMLLRSYDTPNYLPQIPFFWKTSVPNRYAFKK 273
            +L  LL+      +K   LL     + + +LRS+DTPN +P + +FWK+ + NR+  + 
Sbjct: 243 RVLEGLLSAYELSMDKR--LLNKAKHVADFILRSFDTPNRIPILKYFWKSDLRNRFPDRT 300

Query: 274 TKTSMLATNILEFLTLSHITNDPQFDSVVSHSLQTMSRSVLLFDIDYLFPSFVDASGCKP 333
             +  L T  L F+ LS +T   ++   V     T+ +S   FD++++ P  VDASGC+ 
Sbjct: 301 VPSGQLTTMALAFIRLSQLTRLNKYFDAVERVFTTIRQSYNEFDMEFMLPDVVDASGCQL 360

Query: 334 LSDEKIAKGDHLRESNVLKSM-QNGKYIHC----------VIKESLLPQHNVDGNPITLD 382
           L+ E+I  G HL+ S+++KS+ +N K++HC          +   SL  Q       I   
Sbjct: 361 LTQEEIENGAHLKGSSIMKSINENFKFVHCQQLGKFLNPPIDDNSLQEQSQYQAYRINEK 420

Query: 383 TIDLYLTVLK-------AYHLNNGN----------------------LFEKRE----AEK 409
           T+ +   + K       +Y + +G+                      + EKR      +K
Sbjct: 421 TVPILENLFKINDLFQSSYDILDGSSKNANAATMDPSIGSEVEAVDEIIEKRNFKDGTKK 480

Query: 410 SITKS-------------LLHSIKMINDTMLFQPWLPSDLED--LLLPTSIKTNIHFDVL 454
             TK+             L +SI  I   M F+P LP   E+       SI T   F   
Sbjct: 481 DSTKNTVGDKSLIDSQTFLTNSISNIFKFMTFRPMLPKQTENKKFNFLNSILTKSQFMPT 540

Query: 455 EDRNEVSIGKEYKVTPDGAKMSSLLSISSVL----------FNDSSLIEQAKAITKGYYE 504
            +  +V+I K Y V+    ++  +L +SS +             SSL+E ++ IT+  + 
Sbjct: 541 TNELDVTIRKSYDVSLYSCRLGGILGLSSRVPHRGGVNTKYILPSSLLEMSEIITESCFM 600

Query: 505 LYKLLD-QIPDVVALDRKLPTFG---MFDPTAKIALIKSGHYKS--NTMQQGVK------ 552
           L +  D  +P    LD           F+   K  +I +G Y++  N +  G+K      
Sbjct: 601 LMEEFDGLLPQKFELDPCTDETNGNCEFNGETKSRMIANGEYETFENDLDVGIKVSNYGK 660

Query: 553 ----------------ITET------TEDKSKHYTNTD-------------AHSFSPNVK 577
                           ITET      T   SK     D                  P++ 
Sbjct: 661 GGNDQKAKRNVLSKDGITETQNIKGDTVGSSKSIAEIDGDEVTQIRRVFTLGKDIKPHIT 720

Query: 578 DINEEQLCWKLH-TWPFYVNDMVVSTRPPFELIESLIYLYKITEDYQWKLIGE 629
             +     WK H  WPF+VN +         +IES+ Y+Y+I+ + +W+ +G+
Sbjct: 721 TDDTMGSQWKNHPDWPFWVNKVESRRLLDSNIIESIFYMYRISGEQKWRSMGK 773


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.318    0.135    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,512,067
Number of Sequences: 60738
Number of extensions: 1869414
Number of successful extensions: 5066
Number of sequences better than 1.0e-05: 1
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 5061
Number of HSP's gapped (non-prelim): 3
length of query: 703
length of database: 30,389,216
effective HSP length: 113
effective length of query: 590
effective length of database: 23,525,822
effective search space: 13880234980
effective search space used: 13880234980
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)