ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI2323 good R KOG2862 General function prediction only Alanine-glyoxylate aminotransferase AGT1
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI2323 832335 831235 -367
(367 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YFL030w [R] KOG2862 Alanine-glyoxylate aminotransferase AGT1 372 e-103
At2g13360 [R] KOG2862 Alanine-glyoxylate aminotransferase AGT1 129 5e-30
7290721 [R] KOG2862 Alanine-glyoxylate aminotransferase AGT1 124 2e-28
Hs4557289 [R] KOG2862 Alanine-glyoxylate aminotransferase AGT1 115 8e-26
CE23985 [R] KOG2862 Alanine-glyoxylate aminotransferase AGT1 114 3e-25
>YFL030w [R] KOG2862 Alanine-glyoxylate aminotransferase AGT1
Length = 385
Score = 372 bits (956), Expect = e-103
Identities = 199/373 (53%), Positives = 262/373 (69%), Gaps = 11/373 (2%)
Query: 3 DLLLIPGPIMVSSAVEEAASIQSLAHTSPEFVKIFQGALQKLRTVFQTSNAG-DQPIVIS 61
D LLIPGPI++S AV++A + SL HTSPEFV IFQ L+ R VF+++ A QP V++
Sbjct: 6 DTLLIPGPIILSGAVQKALDVPSLGHTSPEFVSIFQRVLKNTRAVFKSAAASKSQPFVLA 65
Query: 62 GSGTLGWDIVGNNLLKS---GDSALTISTGFFSDSFAECLKTYGADVDVLKS-EPGKVVA 117
GSGTLGWDI +N + S + L +STG FSD FA+CL++YGA VDV++ + G+ V
Sbjct: 66 GSGTLGWDIFASNFILSKAPNKNVLVVSTGTFSDRFADCLRSYGAQVDVVRPLKIGESVP 125
Query: 118 LDKIESQLKSKKYQLITLTHVDTSTATLLDLPSITSLVKKVSPETFIIVDGVCSIGCEEF 177
L+ I +L Y +T+THVDTSTA L DL +I+ +K+ SPETF +VD VCSIGCEEF
Sbjct: 126 LELITEKLSQNSYGAVTVTHVDTSTAVLSDLKAISQAIKQTSPETFFVVDAVCSIGCEEF 185
Query: 178 KFSEWSVDFVLTASQKALSCPSGLSVSLISERALSYALS---NESTHGFYANLKKWIPIM 234
+F EW VDF LTASQKA+ P+GLS+SL S R + YAL+ N HG++++L++W PIM
Sbjct: 186 EFDEWGVDFALTASQKAIGAPAGLSISLCSSRFMDYALNDSKNGHVHGYFSSLRRWTPIM 245
Query: 235 KAYEAGVGQYFATPSIQLVNALNVSLGEILSYGLEARIAKNKKTSDQFKNNL-KQLGLTL 293
+ YEAG G YFATP +QL+N+L+V+L EIL GL R +++ SD FK++L L LT
Sbjct: 246 ENYEAGKGAYFATPPVQLINSLDVALKEILEEGLHKRWDLHREMSDWFKDSLVNGLQLTS 305
Query: 294 VSE--DSNSAHGLTAVYVDQPGKVISSLKSKGIVIAGGILPGIKDKYIRIGHMGISAIDD 351
VS + SAHGLTAVYV P VI+ LKS G+VIAGGI I KYIRIGHMG++A +
Sbjct: 306 VSRYPSNMSAHGLTAVYVADPPDVIAFLKSHGVVIAGGIHKDIGPKYIRIGHMGVTACNK 365
Query: 352 NLHHIDKVFEAIK 364
NL ++ F+ IK
Sbjct: 366 NLPYMKNCFDLIK 378
>At2g13360 [R] KOG2862 Alanine-glyoxylate aminotransferase AGT1
Length = 401
Score = 129 bits (325), Expect = 5e-30
Identities = 96/348 (27%), Positives = 169/348 (47%), Gaps = 14/348 (4%)
Query: 5 LLIPGPIMVSSAVEEAASIQSLAHTSPEFVKIFQGALQKLRTVFQTSNAGDQPIVISGSG 64
L +PGP+ + V A + + + SP + + L+ ++ +F+T++ P + +G
Sbjct: 12 LFVPGPVNIPEPVIRAMNRNNEDYRSPAIPALTKTLLEDVKKIFKTTSG--TPFLFPTTG 69
Query: 65 TLGWDIVGNNLLKSGDSALTISTGFFSDSFAECLKTYGADVDVLKSEPGKVVALDKIESQ 124
T W+ N L GD ++ G FS + + K +VDV++S+ G+ L + S+
Sbjct: 70 TGAWESALTNTLSPGDRIVSFLIGQFSLLWIDQQKRLNFNVDVVESDWGQGANLQVLASK 129
Query: 125 LKSKK---YQLITLTHVDTSTATLLDLPSITSLVKKVSPETFIIVDGVCSIGCEEFKFSE 181
L + + I + H +T+T D+ ++ +L+ ++VDGV SI +F+ E
Sbjct: 130 LSQDENHTIKAICIVHNETATGVTNDISAVRTLLDHYKHPALLLVDGVSSICALDFRMDE 189
Query: 182 WSVDFVLTASQKALSCPSGLSVSLISERALSYALSNESTHGFYANLKKWIPIMKAYEAGV 241
W VD LT SQKALS P+GL + S +AL +++S F+ W +K Y+ G
Sbjct: 190 WGVDVALTGSQKALSLPTGLGIVCASPKALEATKTSKSLKVFF----DWNDYLKFYKLGT 245
Query: 242 GQYFATPSIQLVNALNVSLGEILSYGLEARIAKNKKTSDQFKNNLKQLGL-TLVSEDSNS 300
+ TPSIQL+ L +L I GLE IA++ + + ++ GL ++
Sbjct: 246 -YWPYTPSIQLLYGLRAALDLIFEEGLENIIARHARLGKATRLAVEAWGLKNCTQKEEWI 304
Query: 301 AHGLTAVYVD---QPGKVISSLKSKGIVIAGGILPGIKDKYIRIGHMG 345
++ +TAV V +++ + + G L + K RIGH+G
Sbjct: 305 SNTVTAVMVPPHIDGSEIVRRAWQRYNLSLGLGLNKVAGKVFRIGHLG 352
>7290721 [R] KOG2862 Alanine-glyoxylate aminotransferase AGT1
Length = 394
Score = 124 bits (312), Expect = 2e-28
Identities = 104/382 (27%), Positives = 186/382 (48%), Gaps = 34/382 (8%)
Query: 2 SDLLLIPGPIMVSSAVEEAASIQSLAHTSPEFVKIFQGALQKLRTVFQTSNAGDQPIVIS 61
S L+ PGP S V EA S L H PE ++I + ++ +FQT N D + IS
Sbjct: 17 SKTLMGPGPSNCSHRVLEAMSNPVLGHMHPECLQIMDEVKEGIKYIFQTLN--DATMCIS 74
Query: 62 GSGTLGWDIVGNNLLKSGDSALTISTGFFSDSFAECLKTYGADVDVLKSEPGKVVALDKI 121
G+G G + NL++ GD L TG + + + YGA+V +++ G+ ++ ++I
Sbjct: 75 GAGHSGMEAALCNLIEDGDVVLMGITGVWGHRAGDMARRYGAEVHYVEASFGRALSHEEI 134
Query: 122 ESQLKSKKYQLITLTHVDTSTATLL-DLPSITSLVKKVSPETFIIVDGVCSIGCEEFKFS 180
++ + ++ + D+ST + ++ + L +K + F+IVD V S+G EF
Sbjct: 135 TFAFEAHRPKVFFIAQGDSSTGIIQQNIRELGELCRKY--DCFLIVDTVASLGGTEFLMD 192
Query: 181 EWSVDFVLTASQKALSCPSGLSVSLISERALSYALSNESTHGFYANLKKWIPIMKAYEAG 240
EW VD T SQK+L P+GL+ S+RAL+ ++ Y + I+
Sbjct: 193 EWKVDVAYTGSQKSLGGPAGLTPISFSKRALTRIRKRKTKPKVY-----YFDILL----- 242
Query: 241 VGQY---FATPSI-------QLVNALNVSLGEILSYGLEARIAKNKKTSDQFKNNLKQLG 290
+GQY + TP I L+ L +L + GL+A + ++++ S + + +++LG
Sbjct: 243 IGQYWGCYGTPRIYHHTISSTLLYGLREALAHFCAVGLKAVVRRHQECSKRLQLGIEELG 302
Query: 291 LTL-VSEDSNSAHGLTAVY----VDQPGKVISSLKSKGIVIAGGILPGIKDKYIRIGHMG 345
L + VS + + + VD +++ + I+GG+ P ++ + RIG MG
Sbjct: 303 LEMFVSREEERLPTVNTIKVPFGVDWKKVAEYAMRKYSVEISGGLGPTVEHVF-RIGLMG 361
Query: 346 ISAIDDNLHHIDKVFEAIKESI 367
+A + +D V + E+I
Sbjct: 362 ENA---TVERVDMVLSILNEAI 380
>Hs4557289 [R] KOG2862 Alanine-glyoxylate aminotransferase AGT1
Length = 392
Score = 115 bits (289), Expect = 8e-26
Identities = 103/372 (27%), Positives = 170/372 (45%), Gaps = 15/372 (4%)
Query: 2 SDLLLIPGPIMVSSAVEEAASIQSLAHTSPEFVKIFQGALQKLRTVFQTSNAGDQPIVIS 61
+ LLL PGP + + A +Q + S + +I + ++ VFQT N +VIS
Sbjct: 22 NQLLLGPGPSNLPPRIMAAGGLQMIGSMSKDMYQIMDEIKEGIQYVFQTRNP--LTLVIS 79
Query: 62 GSGTLGWDIVGNNLLKSGDSALTISTGFFSDSFAECLKTYGADVDVLKSEPGKVVALDKI 121
GSG + N+L+ GDS L + G + + + GA V + +PG L ++
Sbjct: 80 GSGHCALEAALVNVLEPGDSFLVGANGIWGQRAVDIGERIGARVHPMTKDPGGHYTLQEV 139
Query: 122 ESQLKSKKYQLITLTHVDTSTATLLDLPSITSLVKKVSPETFIIVDGVCSIGCEEFKFSE 181
E L K L+ LTH ++ST L L L + + ++VD V S+G
Sbjct: 140 EEGLAQHKPVLLFLTHGESSTGVLQPLDGFGELCHRY--KCLLLVDSVASLGGTPLYMDR 197
Query: 182 WSVDFVLTASQKALSCPSGLSVSLISERALSYALSNES-THGFYANLKKWIPIMKAYEAG 240
+D + + SQKAL+ P G S+ S++A S ++ FY ++ KW+ +
Sbjct: 198 QGIDILYSGSQKALNAPPGTSLISFSDKAKKKMYSRKTKPFSFYLDI-KWLANFWGCDDQ 256
Query: 241 VGQYFATPSIQLVNALNVSLGEILSYGLEARIAKNKKTSDQFKNNLKQLGLTL-VSEDSN 299
Y T + + +L SL I GLE ++++ + L+ LGL L V + +
Sbjct: 257 PRMYHHTIPVISLYSLRESLALIAEQGLENSWRQHREAAAYLHGRLQALGLQLFVKDPAL 316
Query: 300 SAHGLTAVYV----DQPGKVISSLKSKGIVIAGGILPGIKDKYIRIGHMGISAIDDNLHH 355
+T V V D V + I I GG+ P K +RIG +G +A +N
Sbjct: 317 RLPTVTTVAVPAGYDWRDIVSYVIDHFDIEIMGGLGPS-TGKVLRIGLLGCNATREN--- 372
Query: 356 IDKVFEAIKESI 367
+D+V EA++ ++
Sbjct: 373 VDRVTEALRAAL 384
>CE23985 [R] KOG2862 Alanine-glyoxylate aminotransferase AGT1
Length = 405
Score = 114 bits (284), Expect = 3e-25
Identities = 101/365 (27%), Positives = 162/365 (43%), Gaps = 14/365 (3%)
Query: 8 PGPIMVSSAVEEAASIQSLAHTSPEFVKIFQGALQKLRTVFQTSNAGDQPIVISGSGTLG 67
PGP ++ ++ E S L H PEFV+I L+ VF+T N +SG+G G
Sbjct: 45 PGPSNMADSIAETQSRNLLGHLHPEFVQIMADVRLGLQYVFKTDNK--YTFAVSGTGHSG 102
Query: 68 WDIVGNNLLKSGDSALTISTGFFSDSFAECLKTYGADVDVLKSEPGKVVALDKIESQLKS 127
+ NLL+ GD L + G + A+ G +V + + G+ V ++ I +
Sbjct: 103 MECAMVNLLEPGDKFLVVEIGLWGQRAADLANRMGIEVKKITAPQGQAVPVEDIRKAIAD 162
Query: 128 KKYQLITLTHVDTSTATLLDLPSITSLVKKVSPETFIIVDGVCSIGCEEFKFSEWSVDFV 187
K L+ + D+ST L +I ++ +VD V S+G F + VD V
Sbjct: 163 YKPNLVFVCQGDSSTGVAQPLETIGDACREHG--ALFLVDTVASLGGTPFAADDLKVDCV 220
Query: 188 LTASQKALSCPSGLSVSLISERALSYALS-NESTHGFYANLKKWIPIMKAYEAGVGQYFA 246
+A+QK L+ P GL+ S+RA+ + + FY + + + + + +Y
Sbjct: 221 YSATQKVLNAPPGLAPISFSDRAMEKIRNRKQRVASFYFDAIE-LGNYWGCDGELKRYHH 279
Query: 247 TPSIQLVNALNVSLGEILSYGLEARIAKNKKTSDQFKNNLKQLGL-TLVSEDSNSAHGLT 305
T I V AL +L I G++ I ++K + LK+ GL V ++ LT
Sbjct: 280 TAPISTVYALRAALSAIAKEGIDESIQRHKDNAQVLYATLKKHGLEPFVVDEKLRLPCLT 339
Query: 306 AVYVDQP---GKVISSLKSKGIVIAGGILPGIKDKYIRIGHMGISAIDDNLHHIDKVFEA 362
V V + V + + G IAGG L K RIG GI++ N I+ V E
Sbjct: 340 TVKVPEGVDWKDVAGKMMTNGTEIAGG-LGATVGKIWRIGTFGINS---NSTKIENVVEL 395
Query: 363 IKESI 367
+ +SI
Sbjct: 396 LSKSI 400
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.133 0.369
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,513,722
Number of Sequences: 60738
Number of extensions: 827307
Number of successful extensions: 2378
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 2359
Number of HSP's gapped (non-prelim): 5
length of query: 367
length of database: 30,389,216
effective HSP length: 108
effective length of query: 259
effective length of database: 23,829,512
effective search space: 6171843608
effective search space used: 6171843608
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)